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Naoki Kawada - One of the best experts on this subject based on the ideXlab platform.
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original articleactivation of transient receptor potential vanilloid 3 regulates inflammatory actions of human epidermal keratinocytes
Journal of Investigative Dermatology, 2017Co-Authors: Attila Gabor Szollősi, Nikolett Vasas, Agnes Angyal, Kornel Kistamas, Peter P Nanasi, Johanna Mihaly, Gabriella Beke, Erika Herczeglisztes, Andrea Szegedi, Naoki KawadaAbstract:Transient receptor potential (TRP) ion channels were first characterized on neurons, where they are classically implicated in sensory functions; however, research in recent decades has shown that many of these channels are also expressed on Non-Neuronal Cell types. Emerging findings have highlighted the role of TRP channels in the skin, where they have been shown to be important in numerous cutaneous functions. Of particular interest is TRPV3, which was first described on keratinocytes. Its functional importance was supported when its gain-of-function mutation was linked to Olmsted syndrome, which is characterized by palmoplantar keratoderma, periorifacial hyperkeratosis, diffuse hypotrichosis and alopecia, as well as itch. In spite of these exciting results, we have no information about the role and functionality of TRPV3 on keratinocytes at the Cellular level. In our current study, we have identified TRPV3 expression both on human skin and cultured epidermal keratinocytes. TRPV3 stimulation was found to function as a Ca2+-permeable ion channel which suppresses proliferation of epidermal keratinocytes and induces Cell death. Stimulation of the channel also triggers a strong proinflammatory response via the NF-κB pathway. Collectively our data shows that TRPV3 is functionally expressed on human epidermal keratinocytes and that it plays a role in cutaneous inflammatory processes.
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activation of transient receptor potential vanilloid 3 regulates inflammatory actions of human epidermal keratinocytes
Journal of Investigative Dermatology, 2017Co-Authors: Attila Gabor Szollősi, Nikolett Vasas, Agnes Angyal, Kornel Kistamas, Peter P Nanasi, Johanna Mihaly, Gabriella Beke, Erika Herczeglisztes, Andrea Szegedi, Naoki KawadaAbstract:Abstract Transient receptor potential (TRP) ion channels were first characterized on neurons, where they are classically implicated in sensory functions; however, research in recent decades has shown that many of these channels are also expressed on Non-Neuronal Cell types. Emerging findings have highlighted the role of TRP channels in the skin, where they have been shown to be important in numerous cutaneous functions. Of particular interest is TRPV3, which was first described on keratinocytes. Its functional importance was supported when its gain-of-function mutation was linked to Olmsted syndrome, which is characterized by palmoplantar keratoderma, periorifacial hyperkeratosis, diffuse hypotrichosis and alopecia, as well as itch. In spite of these exciting results, we have no information about the role and functionality of TRPV3 on keratinocytes at the Cellular level. In our current study, we have identified TRPV3 expression both on human skin and cultured epidermal keratinocytes. TRPV3 stimulation was found to function as a Ca 2+ -permeable ion channel which suppresses proliferation of epidermal keratinocytes and induces Cell death. Stimulation of the channel also triggers a strong proinflammatory response via the NF-κB pathway. Collectively our data shows that TRPV3 is functionally expressed on human epidermal keratinocytes and that it plays a role in cutaneous inflammatory processes.
Ali Şahin - One of the best experts on this subject based on the ideXlab platform.
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AB0049 Evaluation of caspase-3, caspase-9, caspase-14 and pannexin-1 levels in behÇet’s disease
Annals of the Rheumatic Diseases, 2018Co-Authors: Abdullah Ilhan, Mehmet Emin Derin, I. Karadag, Halef Okan Doğan, A. C. Urhan, Mehtap Şahin, Ali ŞahinAbstract:Background Behcet’s disease (BD) is a rheumatic disease in which various systemic findings such as especially recurrent oral afts, and genital ulcers, eye involvement, skin lesions, gastrointestinal involvement, neurological involvement, vascular involvement and arthritis can be seen.1 The pathogenesis of the disease has not yet been fully understood. Caspases play a central role in apoptotic signal transduction. They are cysteine bound aspartate specific proteases.2 There are three main types of caspases, the initiator caspases, the effector caspases and the inflammatory caspases. Pannexin-1 is a high-conduction voltage-gated channel protein that is commonly found in many organs and tissues including sensory systems and both neuronal and Non-Neuronal Cell types.3 Objectives The aim of this study is to examine the role of initiator, effector and inflammatory caspases in BD and the levels of pannexin duct protein, which is thought to be active in inflammation in BD and to compare clinical findings with healthy individuals. Methods Between January 2017 – June 2017, fourty-six patients diagnosed with BD admitting to Cumhuriyet University Medical Faculty, Department of Internal Medicine Rheumatology and forty-four healthy volunteers without any rheumatic, systemic and metabolic diseases enrolled in this study. Clinical findings of all patients were recorded. The blood from a peripheral vein using suitable blood tubes was withdrew to measure serum caspase-3, caspase-9, caspase-14 and pannexin-1 levels. Blood tests were examined by Elisa method in Cumhuriyet University Department of Biochemistry. Results The mean serum caspase-3 level was measured as 12.04 (11.25–43.69) pg/ml in BD group and 12.1 (11.19–484.3) pg/ml in healthy control (HC) group. There was no statistically significant difference between two groups (p=0.143). The mean serum levels of caspase-9 in the BD group were measured as 22 (5.14–29.33) pg/ml and 22.01 (11.23–850) pg/ml in the HC group. There was no statistically significant difference between the two groups (p=0.593). The mean serum caspase-14 level was 6 (5.2–8.21) pg/ml in the BD group and 6.15 (5.7–353) pg/ml in the HC group. There was no statistically significant difference between the two groups (p=0.053). The mean serum pannexin-1 levels were 6.36 (4.21–527.2) pg/ml in the BD group and 255.8 (5.38–2000) pg/ml in the HC. Serum pannexin-1 levels were statistically significant higher in the HC group (p Conclusions Serum caspase-3, caspase-9 and caspase-14 levels were not statistically significant different between BD and HC groups. Serum pannexin-1 levels were statistically significant lower in the BD group. References [1] Davatchi F, Chams-Davatchi C, Shams H, Shahram F, Nadji A, Akhlaghi M, et al. Behcet’s disease: epidemiology, clinical manifestations, and diagnosis. Expert Rev Clin Immunol2017Jan 2;13(1):57–65. [2] Green DR, Llambi F. Cell Death Signaling. Cold Spring Harb Perspect Biol. 2015Dec 1;7(12):a006080. [3] Ishikawa M, Yamada Y. The Role of Pannexin 3 in Bone Biology. J Dent Res. 2017Apr 13;96(4):3729. Acknowledgements We would like to thank to ”Cumhuriyet University Scientifical Projects Unit (CUBAP)” for funding this project. Disclosure of Interest None declared
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ab0049 evaluation of caspase 3 caspase 9 caspase 14 and pannexin 1 levels in behcet s disease
Annals of the Rheumatic Diseases, 2018Co-Authors: Abdullah Ilhan, Mehmet Emin Derin, I. Karadag, Halef Okan Doğan, A. C. Urhan, Mehtap Şahin, Ali ŞahinAbstract:Background Behcet’s disease (BD) is a rheumatic disease in which various systemic findings such as especially recurrent oral afts, and genital ulcers, eye involvement, skin lesions, gastrointestinal involvement, neurological involvement, vascular involvement and arthritis can be seen.1 The pathogenesis of the disease has not yet been fully understood. Caspases play a central role in apoptotic signal transduction. They are cysteine bound aspartate specific proteases.2 There are three main types of caspases, the initiator caspases, the effector caspases and the inflammatory caspases. Pannexin-1 is a high-conduction voltage-gated channel protein that is commonly found in many organs and tissues including sensory systems and both neuronal and Non-Neuronal Cell types.3 Objectives The aim of this study is to examine the role of initiator, effector and inflammatory caspases in BD and the levels of pannexin duct protein, which is thought to be active in inflammation in BD and to compare clinical findings with healthy individuals. Methods Between January 2017 – June 2017, fourty-six patients diagnosed with BD admitting to Cumhuriyet University Medical Faculty, Department of Internal Medicine Rheumatology and forty-four healthy volunteers without any rheumatic, systemic and metabolic diseases enrolled in this study. Clinical findings of all patients were recorded. The blood from a peripheral vein using suitable blood tubes was withdrew to measure serum caspase-3, caspase-9, caspase-14 and pannexin-1 levels. Blood tests were examined by Elisa method in Cumhuriyet University Department of Biochemistry. Results The mean serum caspase-3 level was measured as 12.04 (11.25–43.69) pg/ml in BD group and 12.1 (11.19–484.3) pg/ml in healthy control (HC) group. There was no statistically significant difference between two groups (p=0.143). The mean serum levels of caspase-9 in the BD group were measured as 22 (5.14–29.33) pg/ml and 22.01 (11.23–850) pg/ml in the HC group. There was no statistically significant difference between the two groups (p=0.593). The mean serum caspase-14 level was 6 (5.2–8.21) pg/ml in the BD group and 6.15 (5.7–353) pg/ml in the HC group. There was no statistically significant difference between the two groups (p=0.053). The mean serum pannexin-1 levels were 6.36 (4.21–527.2) pg/ml in the BD group and 255.8 (5.38–2000) pg/ml in the HC. Serum pannexin-1 levels were statistically significant higher in the HC group (p Conclusions Serum caspase-3, caspase-9 and caspase-14 levels were not statistically significant different between BD and HC groups. Serum pannexin-1 levels were statistically significant lower in the BD group. References [1] Davatchi F, Chams-Davatchi C, Shams H, Shahram F, Nadji A, Akhlaghi M, et al. Behcet’s disease: epidemiology, clinical manifestations, and diagnosis. Expert Rev Clin Immunol2017Jan 2;13(1):57–65. [2] Green DR, Llambi F. Cell Death Signaling. Cold Spring Harb Perspect Biol. 2015Dec 1;7(12):a006080. [3] Ishikawa M, Yamada Y. The Role of Pannexin 3 in Bone Biology. J Dent Res. 2017Apr 13;96(4):3729. Acknowledgements We would like to thank to ”Cumhuriyet University Scientifical Projects Unit (CUBAP)” for funding this project. Disclosure of Interest None declared
Attila Gabor Szollősi - One of the best experts on this subject based on the ideXlab platform.
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original articleactivation of transient receptor potential vanilloid 3 regulates inflammatory actions of human epidermal keratinocytes
Journal of Investigative Dermatology, 2017Co-Authors: Attila Gabor Szollősi, Nikolett Vasas, Agnes Angyal, Kornel Kistamas, Peter P Nanasi, Johanna Mihaly, Gabriella Beke, Erika Herczeglisztes, Andrea Szegedi, Naoki KawadaAbstract:Transient receptor potential (TRP) ion channels were first characterized on neurons, where they are classically implicated in sensory functions; however, research in recent decades has shown that many of these channels are also expressed on Non-Neuronal Cell types. Emerging findings have highlighted the role of TRP channels in the skin, where they have been shown to be important in numerous cutaneous functions. Of particular interest is TRPV3, which was first described on keratinocytes. Its functional importance was supported when its gain-of-function mutation was linked to Olmsted syndrome, which is characterized by palmoplantar keratoderma, periorifacial hyperkeratosis, diffuse hypotrichosis and alopecia, as well as itch. In spite of these exciting results, we have no information about the role and functionality of TRPV3 on keratinocytes at the Cellular level. In our current study, we have identified TRPV3 expression both on human skin and cultured epidermal keratinocytes. TRPV3 stimulation was found to function as a Ca2+-permeable ion channel which suppresses proliferation of epidermal keratinocytes and induces Cell death. Stimulation of the channel also triggers a strong proinflammatory response via the NF-κB pathway. Collectively our data shows that TRPV3 is functionally expressed on human epidermal keratinocytes and that it plays a role in cutaneous inflammatory processes.
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activation of transient receptor potential vanilloid 3 regulates inflammatory actions of human epidermal keratinocytes
Journal of Investigative Dermatology, 2017Co-Authors: Attila Gabor Szollősi, Nikolett Vasas, Agnes Angyal, Kornel Kistamas, Peter P Nanasi, Johanna Mihaly, Gabriella Beke, Erika Herczeglisztes, Andrea Szegedi, Naoki KawadaAbstract:Abstract Transient receptor potential (TRP) ion channels were first characterized on neurons, where they are classically implicated in sensory functions; however, research in recent decades has shown that many of these channels are also expressed on Non-Neuronal Cell types. Emerging findings have highlighted the role of TRP channels in the skin, where they have been shown to be important in numerous cutaneous functions. Of particular interest is TRPV3, which was first described on keratinocytes. Its functional importance was supported when its gain-of-function mutation was linked to Olmsted syndrome, which is characterized by palmoplantar keratoderma, periorifacial hyperkeratosis, diffuse hypotrichosis and alopecia, as well as itch. In spite of these exciting results, we have no information about the role and functionality of TRPV3 on keratinocytes at the Cellular level. In our current study, we have identified TRPV3 expression both on human skin and cultured epidermal keratinocytes. TRPV3 stimulation was found to function as a Ca 2+ -permeable ion channel which suppresses proliferation of epidermal keratinocytes and induces Cell death. Stimulation of the channel also triggers a strong proinflammatory response via the NF-κB pathway. Collectively our data shows that TRPV3 is functionally expressed on human epidermal keratinocytes and that it plays a role in cutaneous inflammatory processes.
Abdullah Ilhan - One of the best experts on this subject based on the ideXlab platform.
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AB0049 Evaluation of caspase-3, caspase-9, caspase-14 and pannexin-1 levels in behÇet’s disease
Annals of the Rheumatic Diseases, 2018Co-Authors: Abdullah Ilhan, Mehmet Emin Derin, I. Karadag, Halef Okan Doğan, A. C. Urhan, Mehtap Şahin, Ali ŞahinAbstract:Background Behcet’s disease (BD) is a rheumatic disease in which various systemic findings such as especially recurrent oral afts, and genital ulcers, eye involvement, skin lesions, gastrointestinal involvement, neurological involvement, vascular involvement and arthritis can be seen.1 The pathogenesis of the disease has not yet been fully understood. Caspases play a central role in apoptotic signal transduction. They are cysteine bound aspartate specific proteases.2 There are three main types of caspases, the initiator caspases, the effector caspases and the inflammatory caspases. Pannexin-1 is a high-conduction voltage-gated channel protein that is commonly found in many organs and tissues including sensory systems and both neuronal and Non-Neuronal Cell types.3 Objectives The aim of this study is to examine the role of initiator, effector and inflammatory caspases in BD and the levels of pannexin duct protein, which is thought to be active in inflammation in BD and to compare clinical findings with healthy individuals. Methods Between January 2017 – June 2017, fourty-six patients diagnosed with BD admitting to Cumhuriyet University Medical Faculty, Department of Internal Medicine Rheumatology and forty-four healthy volunteers without any rheumatic, systemic and metabolic diseases enrolled in this study. Clinical findings of all patients were recorded. The blood from a peripheral vein using suitable blood tubes was withdrew to measure serum caspase-3, caspase-9, caspase-14 and pannexin-1 levels. Blood tests were examined by Elisa method in Cumhuriyet University Department of Biochemistry. Results The mean serum caspase-3 level was measured as 12.04 (11.25–43.69) pg/ml in BD group and 12.1 (11.19–484.3) pg/ml in healthy control (HC) group. There was no statistically significant difference between two groups (p=0.143). The mean serum levels of caspase-9 in the BD group were measured as 22 (5.14–29.33) pg/ml and 22.01 (11.23–850) pg/ml in the HC group. There was no statistically significant difference between the two groups (p=0.593). The mean serum caspase-14 level was 6 (5.2–8.21) pg/ml in the BD group and 6.15 (5.7–353) pg/ml in the HC group. There was no statistically significant difference between the two groups (p=0.053). The mean serum pannexin-1 levels were 6.36 (4.21–527.2) pg/ml in the BD group and 255.8 (5.38–2000) pg/ml in the HC. Serum pannexin-1 levels were statistically significant higher in the HC group (p Conclusions Serum caspase-3, caspase-9 and caspase-14 levels were not statistically significant different between BD and HC groups. Serum pannexin-1 levels were statistically significant lower in the BD group. References [1] Davatchi F, Chams-Davatchi C, Shams H, Shahram F, Nadji A, Akhlaghi M, et al. Behcet’s disease: epidemiology, clinical manifestations, and diagnosis. Expert Rev Clin Immunol2017Jan 2;13(1):57–65. [2] Green DR, Llambi F. Cell Death Signaling. Cold Spring Harb Perspect Biol. 2015Dec 1;7(12):a006080. [3] Ishikawa M, Yamada Y. The Role of Pannexin 3 in Bone Biology. J Dent Res. 2017Apr 13;96(4):3729. Acknowledgements We would like to thank to ”Cumhuriyet University Scientifical Projects Unit (CUBAP)” for funding this project. Disclosure of Interest None declared
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ab0049 evaluation of caspase 3 caspase 9 caspase 14 and pannexin 1 levels in behcet s disease
Annals of the Rheumatic Diseases, 2018Co-Authors: Abdullah Ilhan, Mehmet Emin Derin, I. Karadag, Halef Okan Doğan, A. C. Urhan, Mehtap Şahin, Ali ŞahinAbstract:Background Behcet’s disease (BD) is a rheumatic disease in which various systemic findings such as especially recurrent oral afts, and genital ulcers, eye involvement, skin lesions, gastrointestinal involvement, neurological involvement, vascular involvement and arthritis can be seen.1 The pathogenesis of the disease has not yet been fully understood. Caspases play a central role in apoptotic signal transduction. They are cysteine bound aspartate specific proteases.2 There are three main types of caspases, the initiator caspases, the effector caspases and the inflammatory caspases. Pannexin-1 is a high-conduction voltage-gated channel protein that is commonly found in many organs and tissues including sensory systems and both neuronal and Non-Neuronal Cell types.3 Objectives The aim of this study is to examine the role of initiator, effector and inflammatory caspases in BD and the levels of pannexin duct protein, which is thought to be active in inflammation in BD and to compare clinical findings with healthy individuals. Methods Between January 2017 – June 2017, fourty-six patients diagnosed with BD admitting to Cumhuriyet University Medical Faculty, Department of Internal Medicine Rheumatology and forty-four healthy volunteers without any rheumatic, systemic and metabolic diseases enrolled in this study. Clinical findings of all patients were recorded. The blood from a peripheral vein using suitable blood tubes was withdrew to measure serum caspase-3, caspase-9, caspase-14 and pannexin-1 levels. Blood tests were examined by Elisa method in Cumhuriyet University Department of Biochemistry. Results The mean serum caspase-3 level was measured as 12.04 (11.25–43.69) pg/ml in BD group and 12.1 (11.19–484.3) pg/ml in healthy control (HC) group. There was no statistically significant difference between two groups (p=0.143). The mean serum levels of caspase-9 in the BD group were measured as 22 (5.14–29.33) pg/ml and 22.01 (11.23–850) pg/ml in the HC group. There was no statistically significant difference between the two groups (p=0.593). The mean serum caspase-14 level was 6 (5.2–8.21) pg/ml in the BD group and 6.15 (5.7–353) pg/ml in the HC group. There was no statistically significant difference between the two groups (p=0.053). The mean serum pannexin-1 levels were 6.36 (4.21–527.2) pg/ml in the BD group and 255.8 (5.38–2000) pg/ml in the HC. Serum pannexin-1 levels were statistically significant higher in the HC group (p Conclusions Serum caspase-3, caspase-9 and caspase-14 levels were not statistically significant different between BD and HC groups. Serum pannexin-1 levels were statistically significant lower in the BD group. References [1] Davatchi F, Chams-Davatchi C, Shams H, Shahram F, Nadji A, Akhlaghi M, et al. Behcet’s disease: epidemiology, clinical manifestations, and diagnosis. Expert Rev Clin Immunol2017Jan 2;13(1):57–65. [2] Green DR, Llambi F. Cell Death Signaling. Cold Spring Harb Perspect Biol. 2015Dec 1;7(12):a006080. [3] Ishikawa M, Yamada Y. The Role of Pannexin 3 in Bone Biology. J Dent Res. 2017Apr 13;96(4):3729. Acknowledgements We would like to thank to ”Cumhuriyet University Scientifical Projects Unit (CUBAP)” for funding this project. Disclosure of Interest None declared
Jennifer C Peleshok - One of the best experts on this subject based on the ideXlab platform.
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neurotrophic factor changes in the rat thick skin following chronic constriction injury of the sciatic nerve
Molecular Pain, 2012Co-Authors: Jennifer C Peleshok, Alfredo RibeirodasilvaAbstract:Cutaneous peripheral neuropathies have been associated with changes of the sensory fiber innervation in the dermis and epidermis. These changes are mediated in part by the increase in local expression of trophic factors. Increase in target tissue nerve growth factor has been implicated in the promotion of peptidergic afferent and sympathetic efferent sprouting following nerve injury. The primary source of nerve growth factor is Cells found in the target tissue, namely the skin. Recent evidence regarding the release and extraCellular maturation of nerve growth factor indicate that it is produced in its precursor form and matured in the extraCellular space. It is our hypothesis that the precursor form of nerve growth factor should be detectable in those Cell types producing it. To date, limitations in available immunohistochemical tools have restricted efforts in obtaining an accurate distribution of nerve growth factor in the skin of naive animals and those with neuropathic pain lesions. It is the objective of this study to delineate the distribution of the precursor form of nerve growth factor to those Cell types expressing it, as well as to describe its distribution with respect to those nerve fibers responsive to it. We observed a decrease in peptidergic fiber innervation at 1 week after the application of a chronic constriction injury (CCI) to the sciatic nerve, followed by a recovery, correlating with TrkA protein levels. ProNGF expression in CCI animals was significantly higher than in sham-operated controls from 1-4 weeks post-CCI. ProNGF immunoreactivity was increased in mast Cells at 1 week post-CCI and, at later time points, in keratinocytes. P75 expression within the dermis and epidermis was significantly higher in CCI-operated animals than in controls and these changes were localized to neuronal and Non-Neuronal Cell populations using specific markers for each. We describe proNGF expression by Non-Neuronal Cells over time after nerve injury as well as the association of NGF-responsive fibers to proNGF-expressing target tissues. ProNGF expression increases following nerve injury in those Cell types previously suggested to express it.
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Neurotrophic factor changes in the rat thick skin following chronic constriction injury of the sciatic nerve
Molecular Pain, 2012Co-Authors: Jennifer C Peleshok, Alfredo Ribeiro-da-silvaAbstract:Background Cutaneous peripheral neuropathies have been associated with changes of the sensory fiber innervation in the dermis and epidermis. These changes are mediated in part by the increase in local expression of trophic factors. Increase in target tissue nerve growth factor has been implicated in the promotion of peptidergic afferent and sympathetic efferent sprouting following nerve injury. The primary source of nerve growth factor is Cells found in the target tissue, namely the skin. Recent evidence regarding the release and extraCellular maturation of nerve growth factor indicate that it is produced in its precursor form and matured in the extraCellular space. It is our hypothesis that the precursor form of nerve growth factor should be detectable in those Cell types producing it. To date, limitations in available immunohistochemical tools have restricted efforts in obtaining an accurate distribution of nerve growth factor in the skin of naïve animals and those with neuropathic pain lesions. It is the objective of this study to delineate the distribution of the precursor form of nerve growth factor to those Cell types expressing it, as well as to describe its distribution with respect to those nerve fibers responsive to it. Results We observed a decrease in peptidergic fiber innervation at 1 week after the application of a chronic constriction injury (CCI) to the sciatic nerve, followed by a recovery, correlating with TrkA protein levels. ProNGF expression in CCI animals was significantly higher than in sham-operated controls from 1-4 weeks post-CCI. ProNGF immunoreactivity was increased in mast Cells at 1 week post-CCI and, at later time points, in keratinocytes. P75 expression within the dermis and epidermis was significantly higher in CCI-operated animals than in controls and these changes were localized to neuronal and Non-Neuronal Cell populations using specific markers for each. Conclusions We describe proNGF expression by Non-Neuronal Cells over time after nerve injury as well as the association of NGF-responsive fibers to proNGF-expressing target tissues. ProNGF expression increases following nerve injury in those Cell types previously suggested to express it.