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Shari Meyers - One of the best experts on this subject based on the ideXlab platform.

  • runx1 aml 1 and runx2 aml 3 cooperate with prostate derived ets factor to activate transcription from the psa upstream regulatory region
    Journal of Cellular Biochemistry, 2006
    Co-Authors: Marcie Fowler, Erkut Borazanci, Laura L Mcghee, Shannon Walls Pylant, Jill B Williams, Jonathan D Glass, Nathan J Davis, Shari Meyers
    Abstract:

    The RUNX transcription factors (RUNX1, RUNX2, and RUNX3) play essential roles in hematopoiesis and skeletal development. Consistent with these roles in differentiation and Cell cycle, the activity of both RUNX1 and RUNX3 is perturbed in cancer. To determine a role for the RUNX factors in prostate biology, we investigated the expression of RUNX factors in prostate epithelial Cell lines and normal prostate tissue. RUNX1, RUNX2, and RUNX3 were expressed in both normal prostate tissue and an immortalized, Non-Transformed Cell line. We found that prostate cancer-derived Cell lines expressed RUNX1 and RUNX2, but not RUNX3. Next, we sought to identify prostate-specific genes whose expression could be regulated by RUNX proteins. Four consensus RUNX sites are located within the prostate-specific antigen (PSA) regulatory region. Chromatin immunoprecipitation (ChIP) analysis showed that RUNX1 is specifically bound to the PSA regulatory region in LNCaP Cells. RUNX1 and RUNX2 activated the PSA regulatory region alone or cooperatively with prostate-derived ETS factor (PDEF) and RUNX1 physically associated with PDEF. Taken together, our results suggest that RUNX factors participate in prostate epithelial Cell function and cooperate with an Ets transcription factor to regulate PSA gene expression.

  • runx1 aml 1 and runx2 aml 3 cooperate with prostate derived ets factor to activate transcription from the psa upstream regulatory region
    Journal of Cellular Biochemistry, 2006
    Co-Authors: Marcie Fowler, Erkut Borazanci, Laura L Mcghee, Shannon Walls Pylant, Jill B Williams, Nathan J Davis, Shari Meyers, Jonathan Glass
    Abstract:

    The RUNX transcription factors (RUNX1, RUNX2, and RUNX3) play essential roles in hematopoiesis and skeletal development. Consistent with these roles in differentiation and Cell cycle, the activity of both RUNX1 and RUNX3 is perturbed in cancer. To determine a role for the RUNX factors in prostate biology, we investigated the expression of RUNX factors in prostate epithelial Cell lines and normal prostate tissue. RUNX1, RUNX2, and RUNX3 were expressed in both normal prostate tissue and an immortalized, Non-Transformed Cell line. We found that prostate cancer-derived Cell lines expressed RUNX1 and RUNX2, but not RUNX3. Next, we sought to identify prostate-specific genes whose expression could be regulated by RUNX proteins. Four consensus RUNX sites are located within the prostate-specific antigen (PSA) regulatory region. Chromatin immunoprecipitation (ChIP) analysis showed that RUNX1 is specifically bound to the PSA regulatory region in LNCaP Cells. RUNX1 and RUNX2 activated the PSA regulatory region alone or cooperatively with prostate-derived ETS factor (PDEF) and RUNX1 physically associated with PDEF. Taken together, our results suggest that RUNX factors participate in prostate epithelial Cell function and cooperate with an Ets transcription factor to regulate PSA gene expression. J. Cell. Biochem. © 2005 Wiley-Liss, Inc.

Marcie Fowler - One of the best experts on this subject based on the ideXlab platform.

  • runx1 aml 1 and runx2 aml 3 cooperate with prostate derived ets factor to activate transcription from the psa upstream regulatory region
    Journal of Cellular Biochemistry, 2006
    Co-Authors: Marcie Fowler, Erkut Borazanci, Laura L Mcghee, Shannon Walls Pylant, Jill B Williams, Jonathan D Glass, Nathan J Davis, Shari Meyers
    Abstract:

    The RUNX transcription factors (RUNX1, RUNX2, and RUNX3) play essential roles in hematopoiesis and skeletal development. Consistent with these roles in differentiation and Cell cycle, the activity of both RUNX1 and RUNX3 is perturbed in cancer. To determine a role for the RUNX factors in prostate biology, we investigated the expression of RUNX factors in prostate epithelial Cell lines and normal prostate tissue. RUNX1, RUNX2, and RUNX3 were expressed in both normal prostate tissue and an immortalized, Non-Transformed Cell line. We found that prostate cancer-derived Cell lines expressed RUNX1 and RUNX2, but not RUNX3. Next, we sought to identify prostate-specific genes whose expression could be regulated by RUNX proteins. Four consensus RUNX sites are located within the prostate-specific antigen (PSA) regulatory region. Chromatin immunoprecipitation (ChIP) analysis showed that RUNX1 is specifically bound to the PSA regulatory region in LNCaP Cells. RUNX1 and RUNX2 activated the PSA regulatory region alone or cooperatively with prostate-derived ETS factor (PDEF) and RUNX1 physically associated with PDEF. Taken together, our results suggest that RUNX factors participate in prostate epithelial Cell function and cooperate with an Ets transcription factor to regulate PSA gene expression.

  • runx1 aml 1 and runx2 aml 3 cooperate with prostate derived ets factor to activate transcription from the psa upstream regulatory region
    Journal of Cellular Biochemistry, 2006
    Co-Authors: Marcie Fowler, Erkut Borazanci, Laura L Mcghee, Shannon Walls Pylant, Jill B Williams, Nathan J Davis, Shari Meyers, Jonathan Glass
    Abstract:

    The RUNX transcription factors (RUNX1, RUNX2, and RUNX3) play essential roles in hematopoiesis and skeletal development. Consistent with these roles in differentiation and Cell cycle, the activity of both RUNX1 and RUNX3 is perturbed in cancer. To determine a role for the RUNX factors in prostate biology, we investigated the expression of RUNX factors in prostate epithelial Cell lines and normal prostate tissue. RUNX1, RUNX2, and RUNX3 were expressed in both normal prostate tissue and an immortalized, Non-Transformed Cell line. We found that prostate cancer-derived Cell lines expressed RUNX1 and RUNX2, but not RUNX3. Next, we sought to identify prostate-specific genes whose expression could be regulated by RUNX proteins. Four consensus RUNX sites are located within the prostate-specific antigen (PSA) regulatory region. Chromatin immunoprecipitation (ChIP) analysis showed that RUNX1 is specifically bound to the PSA regulatory region in LNCaP Cells. RUNX1 and RUNX2 activated the PSA regulatory region alone or cooperatively with prostate-derived ETS factor (PDEF) and RUNX1 physically associated with PDEF. Taken together, our results suggest that RUNX factors participate in prostate epithelial Cell function and cooperate with an Ets transcription factor to regulate PSA gene expression. J. Cell. Biochem. © 2005 Wiley-Liss, Inc.

William Eustace Johnson - One of the best experts on this subject based on the ideXlab platform.

  • combined bezafibrate medroxyprogesterone acetate and valproic acid treatment inhibits osteosarcoma Cell growth without adversely affecting normal mesenchymal stem Cells
    Bioscience Reports, 2021
    Co-Authors: Jonathan J Sheard, Andrew D Southam, Hannah L Mackay, Max A Ellington, Martyn D Snow, Farhat Khanim, Christopher M Bunce, William Eustace Johnson
    Abstract:

    Drug repurposing is a cost effective means of targeting new therapies for cancer. We have examined the effects of the repurposed drugs, bezafibrate, medroxyprogesterone acetate and valproic acid on human osteosarcoma Cells, i.e., SAOS2 and MG63 compared with their normal Cell counterparts, i.e. mesenchymal stem/stromal Cells (MSCs). Cell growth, viability and migration were measured by biochemical assay and live Cell imaging, whilst levels of lipid-synthesising enzymes were measured by immunoblotting Cell extracts. These drug treatments inhibited the growth and survival of SAOS2 and MG63 Cells most effectively when used in combination (termed V-BAP). In contrast, V-BAP treated MSCs remained viable with only moderately reduced Cell proliferation. V-BAP treatment also inhibited migratory Cell phenotypes. MG63 and SAOS2 Cells expressed much greater levels of fatty acid synthase and stearoyl CoA desaturase 1 than MSCs, but these elevated enzyme levels significantly decreased in the V-BAP treated osteosarcoma Cells prior to Cell death. Hence, we have identified a repurposed drug combination that selectively inhibits the growth and survival of human osteosarcoma Cells in association with altered lipid metabolism without adversely affecting their Non-Transformed Cell counterparts.

Jill B Williams - One of the best experts on this subject based on the ideXlab platform.

  • runx1 aml 1 and runx2 aml 3 cooperate with prostate derived ets factor to activate transcription from the psa upstream regulatory region
    Journal of Cellular Biochemistry, 2006
    Co-Authors: Marcie Fowler, Erkut Borazanci, Laura L Mcghee, Shannon Walls Pylant, Jill B Williams, Jonathan D Glass, Nathan J Davis, Shari Meyers
    Abstract:

    The RUNX transcription factors (RUNX1, RUNX2, and RUNX3) play essential roles in hematopoiesis and skeletal development. Consistent with these roles in differentiation and Cell cycle, the activity of both RUNX1 and RUNX3 is perturbed in cancer. To determine a role for the RUNX factors in prostate biology, we investigated the expression of RUNX factors in prostate epithelial Cell lines and normal prostate tissue. RUNX1, RUNX2, and RUNX3 were expressed in both normal prostate tissue and an immortalized, Non-Transformed Cell line. We found that prostate cancer-derived Cell lines expressed RUNX1 and RUNX2, but not RUNX3. Next, we sought to identify prostate-specific genes whose expression could be regulated by RUNX proteins. Four consensus RUNX sites are located within the prostate-specific antigen (PSA) regulatory region. Chromatin immunoprecipitation (ChIP) analysis showed that RUNX1 is specifically bound to the PSA regulatory region in LNCaP Cells. RUNX1 and RUNX2 activated the PSA regulatory region alone or cooperatively with prostate-derived ETS factor (PDEF) and RUNX1 physically associated with PDEF. Taken together, our results suggest that RUNX factors participate in prostate epithelial Cell function and cooperate with an Ets transcription factor to regulate PSA gene expression.

  • runx1 aml 1 and runx2 aml 3 cooperate with prostate derived ets factor to activate transcription from the psa upstream regulatory region
    Journal of Cellular Biochemistry, 2006
    Co-Authors: Marcie Fowler, Erkut Borazanci, Laura L Mcghee, Shannon Walls Pylant, Jill B Williams, Nathan J Davis, Shari Meyers, Jonathan Glass
    Abstract:

    The RUNX transcription factors (RUNX1, RUNX2, and RUNX3) play essential roles in hematopoiesis and skeletal development. Consistent with these roles in differentiation and Cell cycle, the activity of both RUNX1 and RUNX3 is perturbed in cancer. To determine a role for the RUNX factors in prostate biology, we investigated the expression of RUNX factors in prostate epithelial Cell lines and normal prostate tissue. RUNX1, RUNX2, and RUNX3 were expressed in both normal prostate tissue and an immortalized, Non-Transformed Cell line. We found that prostate cancer-derived Cell lines expressed RUNX1 and RUNX2, but not RUNX3. Next, we sought to identify prostate-specific genes whose expression could be regulated by RUNX proteins. Four consensus RUNX sites are located within the prostate-specific antigen (PSA) regulatory region. Chromatin immunoprecipitation (ChIP) analysis showed that RUNX1 is specifically bound to the PSA regulatory region in LNCaP Cells. RUNX1 and RUNX2 activated the PSA regulatory region alone or cooperatively with prostate-derived ETS factor (PDEF) and RUNX1 physically associated with PDEF. Taken together, our results suggest that RUNX factors participate in prostate epithelial Cell function and cooperate with an Ets transcription factor to regulate PSA gene expression. J. Cell. Biochem. © 2005 Wiley-Liss, Inc.

Nathan J Davis - One of the best experts on this subject based on the ideXlab platform.

  • runx1 aml 1 and runx2 aml 3 cooperate with prostate derived ets factor to activate transcription from the psa upstream regulatory region
    Journal of Cellular Biochemistry, 2006
    Co-Authors: Marcie Fowler, Erkut Borazanci, Laura L Mcghee, Shannon Walls Pylant, Jill B Williams, Jonathan D Glass, Nathan J Davis, Shari Meyers
    Abstract:

    The RUNX transcription factors (RUNX1, RUNX2, and RUNX3) play essential roles in hematopoiesis and skeletal development. Consistent with these roles in differentiation and Cell cycle, the activity of both RUNX1 and RUNX3 is perturbed in cancer. To determine a role for the RUNX factors in prostate biology, we investigated the expression of RUNX factors in prostate epithelial Cell lines and normal prostate tissue. RUNX1, RUNX2, and RUNX3 were expressed in both normal prostate tissue and an immortalized, Non-Transformed Cell line. We found that prostate cancer-derived Cell lines expressed RUNX1 and RUNX2, but not RUNX3. Next, we sought to identify prostate-specific genes whose expression could be regulated by RUNX proteins. Four consensus RUNX sites are located within the prostate-specific antigen (PSA) regulatory region. Chromatin immunoprecipitation (ChIP) analysis showed that RUNX1 is specifically bound to the PSA regulatory region in LNCaP Cells. RUNX1 and RUNX2 activated the PSA regulatory region alone or cooperatively with prostate-derived ETS factor (PDEF) and RUNX1 physically associated with PDEF. Taken together, our results suggest that RUNX factors participate in prostate epithelial Cell function and cooperate with an Ets transcription factor to regulate PSA gene expression.

  • runx1 aml 1 and runx2 aml 3 cooperate with prostate derived ets factor to activate transcription from the psa upstream regulatory region
    Journal of Cellular Biochemistry, 2006
    Co-Authors: Marcie Fowler, Erkut Borazanci, Laura L Mcghee, Shannon Walls Pylant, Jill B Williams, Nathan J Davis, Shari Meyers, Jonathan Glass
    Abstract:

    The RUNX transcription factors (RUNX1, RUNX2, and RUNX3) play essential roles in hematopoiesis and skeletal development. Consistent with these roles in differentiation and Cell cycle, the activity of both RUNX1 and RUNX3 is perturbed in cancer. To determine a role for the RUNX factors in prostate biology, we investigated the expression of RUNX factors in prostate epithelial Cell lines and normal prostate tissue. RUNX1, RUNX2, and RUNX3 were expressed in both normal prostate tissue and an immortalized, Non-Transformed Cell line. We found that prostate cancer-derived Cell lines expressed RUNX1 and RUNX2, but not RUNX3. Next, we sought to identify prostate-specific genes whose expression could be regulated by RUNX proteins. Four consensus RUNX sites are located within the prostate-specific antigen (PSA) regulatory region. Chromatin immunoprecipitation (ChIP) analysis showed that RUNX1 is specifically bound to the PSA regulatory region in LNCaP Cells. RUNX1 and RUNX2 activated the PSA regulatory region alone or cooperatively with prostate-derived ETS factor (PDEF) and RUNX1 physically associated with PDEF. Taken together, our results suggest that RUNX factors participate in prostate epithelial Cell function and cooperate with an Ets transcription factor to regulate PSA gene expression. J. Cell. Biochem. © 2005 Wiley-Liss, Inc.