The Experts below are selected from a list of 63 Experts worldwide ranked by ideXlab platform
Uwe Roesler - One of the best experts on this subject based on the ideXlab platform.
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In vitro susceptibility of Staphylococcus aureus strains isolated from cows with subclinical mastitis to different antimicrobial agents.
Journal of veterinary science, 2012Co-Authors: Ayman El Behiry, Gerd Schlenker, Istvan Szabo, Uwe RoeslerAbstract:Sensitivity to commercial teat dips (Nonoxinol-9 iodine complex and chlorhexidine digluconate) of 56 Staphylococcus (S.) aureus strains isolated from quarter milk samples of various German dairy herds treated with different teat dipping schemes was investigated in this study. The minimum inhibitory concentration was determined using a broth macrodilution method according to the German Veterinary Association guidelines. The main objective of the current study was to induce in vitro resistance induction of S. aureus to chemical disinfectants. Ten different strains were repeatedly passed ten times in growth media with sub-lethal concentrations of disinfectants. Nine strains showed a significant reduction in susceptibility to the Nonoxinol-9 iodine complex but only one strain developed resistance to chlorhexidine digluconate. Stability of the acquired resistance was observed in all S. aureus strains adapted to the Nonoxinol-9 iodine complex and chlorhexidine digluconate. In contrast, simultaneous resistance to different antibiotics was not observed in any of the ten investigated S. aureus strains. However, the isolates exhibited a high degree of resistance to penicillin G. Based on these results, resistance of S. aureus to chemical disinfectants may be more likely to develop if the chemicals are used at concentrations lower than that required for an optimal biocidal effect.
Juan E. Suárez - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of newly isolated human vaginal lactobacilli and selection of probiotic candidates
International Microbiology, 2010Co-Authors: Rebeca Martín, Nora Soberon, Mario Vaneechoutte, Ana B. Florez, Fernando Vazquez, Juan E. SuárezAbstract:The probiotic relevant characteristics of 45 strains of vaginal Lactobacillus isolated from healthy women were analyzed. 21 of them were classified as L. crispatus, 17 as L. jensenii, 6 as L. gasseri and one as L. plantarum. The rate of acidification varied significantly between the strains as did the ability to form biofilms. None used glycogen as a fermentable carbohydrate. Generation of H2O2 was common, being especially frequent among L. jensenii isolates (88%). No bacteriocinogenic strains were detected. Most strains harboured plasmids (between 1 and 7) of various sizes, those in excess of 50 kb being frequent. One of these plasmids was found to be promiscuous since it hybridized with extrachromosomal bands of 15 isolates. All strains were resistant to metronidazole, ciprofloxacin, gentamicin, clindamycin, trimethoprim and sulfametoxazole and susceptible to a series of β-lactams, erythromycin, tetracycline and benzalkonium chloride. Furthermore, no amplification was found with primers that matched a series of tetracycline and erythromycin resistance genes. Almost half of the strains were highly resistant to Nonoxinol 9, which is commonly used as a spermicide. Based on these analysis strains of all three common species are proposed as new probiotic candidates
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Evaluation of newly isolated human vaginal lactobacilli and selection of probiotic candidates
International Microbiology, 2010Co-Authors: Rebeca Ibáñez Martín, Nora Soberon, Mario Vaneechoutte, Ana B. Florez, Fernando Vazquez, Juan E. SuárezAbstract:The probiotic relevant characteristics of 45 strains of vaginal Lactobacillus isolated from healthy women were analyzed. 21 of them were classified as L. crispatus , 17 as L. jensenii , 6 as L. gasseri and one as L. plantarum . The rate of acidification varied significantly between the strains as did the ability to form biofilms. None used glycogen as a fermentable carbohydrate. Generation of H2O2 was common, being especially frequent among L. jensenii isolates (88%). No bacteriocinogenic strains were detected. Most strains harboured plasmids (between 1 and 7) of various sizes, those in excess of 50 kb being frequent. One of these plasmids was found to be promiscuous since it hybridized with extrachromosomal bands of 15 isolates. All strains were resistant to metronidazole, ciprofloxacin, gentamicin, clindamycin, trimethoprim and sulfametoxazole and susceptible to a series of β-lactams, erythromycin, tetracycline and benzalkonium chloride. Furthermore, no amplification was found with primers that matched a series of tetracycline and erythromycin resistance genes. Almost half of the strains were highly resistant to Nonoxinol 9, which is commonly used as a spermicide. Based on these analysis strains of all three common species are proposed as new probiotic candidates.
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Characterization of indigenous vaginal lactobacilli from healthy women as probiotic candidates
International Microbiology, 2009Co-Authors: Rebeca Martín, Nora Soberon, Mario Vaneechoutte, Ana B. Florez, Fernando Vazquez, Juan E. SuárezAbstract:The probiotic relevant characteristics of 45 strains of vaginal Lactobocillus isolated from healthy women were analyzed. Of these, 21 strains were classified as L. crispatus, 17 as L. jensenii, six as L. gasseri, and one as L. plantarum. The rate of acidification varied significantly between the strains as did their ability to form biofilms. None used glycogen as a fermentable carbohydrate. H(2)O(2) generation was common, especially among L. jensenii isolates (88%). No bacteriocinogenic strains were detected. Most strains harbored plasmids (from 1 to 7) of various sizes, those in excess of 50 kb being frequent. One of these plasmids was found to be promiscuous since it hybridized with extrachromosomal bands of 15 isolates. All strains were resistant to metronidazole, ciprofloxacin, gentamicin, clindamycin, trimethoprim, and sulfametoxazole and susceptible to a series of beta-lactams, erythromycin, tetracycline, and benzalkonium chloride. Almost half of the strains were highly resistant to Nonoxinol 9, which is commonly used as a spermicide. Based on these analyses, strains of all three common species are proposed as new probiotic candidates.
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Characterization of indigenous vaginal lactobacilli from healthy women as probiotic candidates.
International microbiology : the official journal of the Spanish Society for Microbiology, 2008Co-Authors: Rebeca Martín, Nora Soberon, Mario Vaneechoutte, Ana B. Florez, Fernando Vazquez, Juan E. SuárezAbstract:Summary. The probiotic relevant characteristics of 45 strains of vaginal Lactobacillus isolated from healthy women were analyzed. Of these, 21 strains were classified as L. crispatus, 17 as L. jensenii, six as L. gasseri, and one as L. plantarum. The rate of acidification varied significantly between the strains as did their ability to form biofilms. None used glycogen as a fermentable carbohydrate. H2O2 generation was common, especially among L. jensenii isolates (88%). No bacteriocinogenic strains were detected. Most strains harbored plasmids (from 1 to 7) of various sizes, those in excess of 50 kb being frequent. One of these plasmids was found to be promiscuous since it hybridized with extrachromosomal bands of 15 isolates. All strains were resistant to metronidazole, ciprofloxacin, gentamicin, clindamycin, trimethoprim, and sulfametoxazole and susceptible to a series of β-lactams, erythromycin, tetracycline, and benzalkonium chloride. Almost half of the strains were highly resistant to Nonoxinol 9, which is commonly used as a spermicide. Based on these analyses, strains of all three common species are proposed as new probiotic candidates. [Int Microbiol 2008; 11(4):261-266]
Ayman El Behiry - One of the best experts on this subject based on the ideXlab platform.
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In vitro susceptibility of Staphylococcus aureus strains isolated from cows with subclinical mastitis to different antimicrobial agents.
Journal of veterinary science, 2012Co-Authors: Ayman El Behiry, Gerd Schlenker, Istvan Szabo, Uwe RoeslerAbstract:Sensitivity to commercial teat dips (Nonoxinol-9 iodine complex and chlorhexidine digluconate) of 56 Staphylococcus (S.) aureus strains isolated from quarter milk samples of various German dairy herds treated with different teat dipping schemes was investigated in this study. The minimum inhibitory concentration was determined using a broth macrodilution method according to the German Veterinary Association guidelines. The main objective of the current study was to induce in vitro resistance induction of S. aureus to chemical disinfectants. Ten different strains were repeatedly passed ten times in growth media with sub-lethal concentrations of disinfectants. Nine strains showed a significant reduction in susceptibility to the Nonoxinol-9 iodine complex but only one strain developed resistance to chlorhexidine digluconate. Stability of the acquired resistance was observed in all S. aureus strains adapted to the Nonoxinol-9 iodine complex and chlorhexidine digluconate. In contrast, simultaneous resistance to different antibiotics was not observed in any of the ten investigated S. aureus strains. However, the isolates exhibited a high degree of resistance to penicillin G. Based on these results, resistance of S. aureus to chemical disinfectants may be more likely to develop if the chemicals are used at concentrations lower than that required for an optimal biocidal effect.
Istvan Szabo - One of the best experts on this subject based on the ideXlab platform.
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In vitro susceptibility of Staphylococcus aureus strains isolated from cows with subclinical mastitis to different antimicrobial agents.
Journal of veterinary science, 2012Co-Authors: Ayman El Behiry, Gerd Schlenker, Istvan Szabo, Uwe RoeslerAbstract:Sensitivity to commercial teat dips (Nonoxinol-9 iodine complex and chlorhexidine digluconate) of 56 Staphylococcus (S.) aureus strains isolated from quarter milk samples of various German dairy herds treated with different teat dipping schemes was investigated in this study. The minimum inhibitory concentration was determined using a broth macrodilution method according to the German Veterinary Association guidelines. The main objective of the current study was to induce in vitro resistance induction of S. aureus to chemical disinfectants. Ten different strains were repeatedly passed ten times in growth media with sub-lethal concentrations of disinfectants. Nine strains showed a significant reduction in susceptibility to the Nonoxinol-9 iodine complex but only one strain developed resistance to chlorhexidine digluconate. Stability of the acquired resistance was observed in all S. aureus strains adapted to the Nonoxinol-9 iodine complex and chlorhexidine digluconate. In contrast, simultaneous resistance to different antibiotics was not observed in any of the ten investigated S. aureus strains. However, the isolates exhibited a high degree of resistance to penicillin G. Based on these results, resistance of S. aureus to chemical disinfectants may be more likely to develop if the chemicals are used at concentrations lower than that required for an optimal biocidal effect.
Gerd Schlenker - One of the best experts on this subject based on the ideXlab platform.
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In vitro susceptibility of Staphylococcus aureus strains isolated from cows with subclinical mastitis to different antimicrobial agents.
Journal of veterinary science, 2012Co-Authors: Ayman El Behiry, Gerd Schlenker, Istvan Szabo, Uwe RoeslerAbstract:Sensitivity to commercial teat dips (Nonoxinol-9 iodine complex and chlorhexidine digluconate) of 56 Staphylococcus (S.) aureus strains isolated from quarter milk samples of various German dairy herds treated with different teat dipping schemes was investigated in this study. The minimum inhibitory concentration was determined using a broth macrodilution method according to the German Veterinary Association guidelines. The main objective of the current study was to induce in vitro resistance induction of S. aureus to chemical disinfectants. Ten different strains were repeatedly passed ten times in growth media with sub-lethal concentrations of disinfectants. Nine strains showed a significant reduction in susceptibility to the Nonoxinol-9 iodine complex but only one strain developed resistance to chlorhexidine digluconate. Stability of the acquired resistance was observed in all S. aureus strains adapted to the Nonoxinol-9 iodine complex and chlorhexidine digluconate. In contrast, simultaneous resistance to different antibiotics was not observed in any of the ten investigated S. aureus strains. However, the isolates exhibited a high degree of resistance to penicillin G. Based on these results, resistance of S. aureus to chemical disinfectants may be more likely to develop if the chemicals are used at concentrations lower than that required for an optimal biocidal effect.
