The Experts below are selected from a list of 2013 Experts worldwide ranked by ideXlab platform
Jose Pedrazachaverri - One of the best experts on this subject based on the ideXlab platform.
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signaling pathways activated by the phytochemical Nordihydroguaiaretic Acid contribute to a keap1 independent regulation of nrf2 stability role of glycogen synthase kinase 3
Free Radical Biology and Medicine, 2012Co-Authors: Jose Pedrazachaverri, Omar N Medinacampos, Ana I Rojo, Patricia Rada, Adverqueydi Zunigatoala, Areli Lopezgazcon, Sandra Espada, Antonio CuadradoAbstract:Abstract Defense against oxidative stress is executed by an antioxidant program that is tightly controlled by the transcription factor Nrf2. The stability of Nrf2 involves the interaction of two degradation domains, designated Neh2 and Neh6, with the E3 ubiquitin ligase adaptors, Keap1 and β-TrCP, respectively. The regulation of Nrf2 through the Neh6 degron remains largely unexplored but requires GSK-3 to form a phosphodegron. In this study, the cancer-chemopreventive agent Nordihydroguaiaretic Acid (NDGA) increased the level of Nrf2 protein and expression of heme oxygenase-1 (HO-1) in kidney-derived LLC-PK1 and HEK293T cells and in wild-type mouse embryo fibroblasts (MEFs). However, NDGA did not induce HO-1 in Nrf2−/− MEFs, indicating that Nrf2 is required for induction. The relevance of the Nrf2/HO-1 axis to antioxidant protection was further demonstrated by the finding that the HO-1 inhibitor stannous-mesoporphyrin abolished protection against hydrogen peroxide conferred by NDGA. NDGA increased Nrf2 and HO-1 protein levels in Keap1−/− MEFs, implying that Keap1-independent mechanisms regulate Nrf2 stability. Mutants of the Neh2 or Nrh6 domain and chimeric proteins comprising cyan fluorescent protein fused to Neh2 and green fluorescent protein fused to Neh6 exhibited longer half-lives in the presence of NDGA, demonstrating that NDGA targets both the Neh2 and the Neh6 degrons. In common with other chemopreventive agents, NDGA activated the ERK1/2, p38, JNK, and PI3K pathways. By using selective kinase inhibitors we found that PI3K, JNK, and p38 were responsible for the stabilization of Nrf2 and induction of HO-1 by NDGA. To explain how NDGA might up-regulate Nrf2 in a Keap1-independent manner we explored the participation of GSK-3β because it controls the Neh6 phosphodegron. Importantly, NDGA caused inhibitory phosphorylation of GSK-3β at Ser9 and at Thr390, and this was associated with a substantial reduction in Neh6 phosphorylation. Our study demonstrates that NDGA activates Nrf2 through multiple signaling cascades and identifies GSK-3β as an integrator of these signaling pathways and a gatekeeper of Nrf2 stability at the level of the Neh6 phosphodegron.
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Nordihydroguaiaretic Acid attenuates potassium dichromate induced oxidative stress and nephrotoxicity
Food and Chemical Toxicology, 2008Co-Authors: Paola Yamcanul, Cleva Villanueva, Dolores Javier Sanchezgonzalez, Yolanda I Chirino, Claudia Maria Martinezmartinez, Cristino Cruz, Jose PedrazachaverriAbstract:Abstract Larrea tridentata also known as Creosote bush, Larrea, chaparral, greasewood or gobernadora has been used in the folk medicine for the treatment of several illnesses. The primary product that is present at high concentrations in the leaves from this plant is Nordihydroguaiaretic Acid (NDGA) which is a powerful antioxidant. On the other hand, potassium dichromate (K 2 Cr 2 O 7 )-induced nephrotoxicity is associated with oxidative stress. The aim of this work was to study the effect of NDGA on K 2 Cr 2 O 7 -induced nephrotoxicity and oxidative stress. Nephrotoxicity was induced by a single injection of K 2 Cr 2 O 7 (15 mg/Kg). A group of K 2 Cr 2 O 7 -treated rats was administered NDGA by mini osmotic pumps (17 mg/Kg/day). The results show that NDGA was able to ameliorate the structural and functional renal damage evaluated by histopathological analysis and by measuring proteinuria, urinary excretion of N -acetyl-β- d -glucosaminidase, serum creatinine, and serum glutathione peroxidase activity. In addition, immunostaining of 4-hydroxy-2-nonenal and 3-nitrotyrosine, markers of oxidative and nitrosative stress, respectively, was ameliorated by the NDGA treatment. These data strongly suggest that the antioxidant properties of NDGA are involved in its renoprotective effect in K 2 Cr 2 O 7 -treated rats.
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Nordihydroguaiaretic Acid is a potent in vitro scavenger of peroxynitrite singlet oxygen hydroxyl radical superoxide anion and hypochlorous Acid and prevents in vivo ozone induced tyrosine nitration in lungs
Free Radical Research, 2006Co-Authors: Esau Florianosanchez, Cleva Villanueva, Omar N Medinacampos, Diana Rocha, Dolores Javier Sanchezgonzalez, Noemi Cardenasrodriguez, Jose PedrazachaverriAbstract:The antioxidant Nordihydroguaiaretic Acid (NDGA) has recently become well known as a putative anticancer drug. In this paper, it was evaluated the in vitro peroxynitrite (ONOO− ), singlet oxygen (1O2), hydroxyl radical (OH√), hydrogen peroxide (H2O2), superoxide anion and hypochlorous Acid (HOCl) scavenging capacity of NDGA. It was found that NDGA scavenges: (a) ONOO− (IC50 = 4 ± 0.94 μM) as efficiently as uric Acid; (b) 1O2 (IC50 = 151 ± 20 μM) more efficiently than dimethyl thiourea, lipoic Acid, N-acetyl-cysteine and glutathione; (c) OH√ (IC50 = 0.15 ± 0.02 μM) more efficiently than dimethyl thiourea, uric Acid, trolox, dimethyl sulfoxide and mannitol, (d) (IC50 = 15 ± 1 μM) more efficiently than N-acetyl-cysteine, glutathione, tempol and deferoxamine and (e) HOCl (IC50 = 622 ± 42 μM) as efficiently as lipoic Acid and N-acetyl-cysteine. NDGA was unable to scavenge H2O2. In an in vivo study in rats, NDGA was able to prevent ozone-induced tyrosine nitration in lungs. It is concluded that NDGA is a potent...
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Nordihydroguaiaretic Acid is a potent in vitro scavenger of peroxynitrite singlet oxygen hydroxyl radical superoxide anion and hypochlorous Acid and prevents in vivo ozone induced tyrosine nitration in lungs
Free Radical Research, 2006Co-Authors: Esau Florianosanchez, Cleva Villanueva, Omar N Medinacampos, Diana Rocha, Dolores Javier Sanchezgonzalez, Noemi Cardenasrodriguez, Jose PedrazachaverriAbstract:The antioxidant Nordihydroguaiaretic Acid (NDGA) has recently become well known as a putative anticancer drug. In this paper, it was evaluated the in vitro peroxynitrite (ONOO(-)), singlet oxygen ((1)O(2)), hydroxyl radical (OH(v)), hydrogen peroxide (H(2)O(2)), superoxide anion and hypochlorous Acid (HOCl) scavenging capacity of NDGA. It was found that NDGA scavenges: (a) ONOO(-) (IC(50) = 4 +/- 0.94 microM) as efficiently as uric Acid; (b) (1)O(2) (IC(50) = 151 +/- 20 microM) more efficiently than dimethyl thiourea, lipoic Acid, N-acetyl-cysteine and glutathione; (c) OH(v) (IC(50) = 0.15 +/- 0.02 microM) more efficiently than dimethyl thiourea, uric Acid, trolox, dimethyl sulfoxide and mannitol, (d) (IC(50) = 15 +/- 1 microM) more efficiently than N-acetyl-cysteine, glutathione, tempol and deferoxamine and (e) HOCl (IC(50) = 622 +/- 42 microM) as efficiently as lipoic Acid and N-acetyl-cysteine. NDGA was unable to scavenge H(2)O(2). In an in vivo study in rats, NDGA was able to prevent ozone-induced tyrosine nitration in lungs. It is concluded that NDGA is a potent in vitro scavenger of ONOO(-), (1)O(2), OH(v), and HOCl and is able to prevent lung tyrosine nitration in vivo.
Omar N Medinacampos - One of the best experts on this subject based on the ideXlab platform.
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signaling pathways activated by the phytochemical Nordihydroguaiaretic Acid contribute to a keap1 independent regulation of nrf2 stability role of glycogen synthase kinase 3
Free Radical Biology and Medicine, 2012Co-Authors: Jose Pedrazachaverri, Omar N Medinacampos, Ana I Rojo, Patricia Rada, Adverqueydi Zunigatoala, Areli Lopezgazcon, Sandra Espada, Antonio CuadradoAbstract:Abstract Defense against oxidative stress is executed by an antioxidant program that is tightly controlled by the transcription factor Nrf2. The stability of Nrf2 involves the interaction of two degradation domains, designated Neh2 and Neh6, with the E3 ubiquitin ligase adaptors, Keap1 and β-TrCP, respectively. The regulation of Nrf2 through the Neh6 degron remains largely unexplored but requires GSK-3 to form a phosphodegron. In this study, the cancer-chemopreventive agent Nordihydroguaiaretic Acid (NDGA) increased the level of Nrf2 protein and expression of heme oxygenase-1 (HO-1) in kidney-derived LLC-PK1 and HEK293T cells and in wild-type mouse embryo fibroblasts (MEFs). However, NDGA did not induce HO-1 in Nrf2−/− MEFs, indicating that Nrf2 is required for induction. The relevance of the Nrf2/HO-1 axis to antioxidant protection was further demonstrated by the finding that the HO-1 inhibitor stannous-mesoporphyrin abolished protection against hydrogen peroxide conferred by NDGA. NDGA increased Nrf2 and HO-1 protein levels in Keap1−/− MEFs, implying that Keap1-independent mechanisms regulate Nrf2 stability. Mutants of the Neh2 or Nrh6 domain and chimeric proteins comprising cyan fluorescent protein fused to Neh2 and green fluorescent protein fused to Neh6 exhibited longer half-lives in the presence of NDGA, demonstrating that NDGA targets both the Neh2 and the Neh6 degrons. In common with other chemopreventive agents, NDGA activated the ERK1/2, p38, JNK, and PI3K pathways. By using selective kinase inhibitors we found that PI3K, JNK, and p38 were responsible for the stabilization of Nrf2 and induction of HO-1 by NDGA. To explain how NDGA might up-regulate Nrf2 in a Keap1-independent manner we explored the participation of GSK-3β because it controls the Neh6 phosphodegron. Importantly, NDGA caused inhibitory phosphorylation of GSK-3β at Ser9 and at Thr390, and this was associated with a substantial reduction in Neh6 phosphorylation. Our study demonstrates that NDGA activates Nrf2 through multiple signaling cascades and identifies GSK-3β as an integrator of these signaling pathways and a gatekeeper of Nrf2 stability at the level of the Neh6 phosphodegron.
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Nordihydroguaiaretic Acid is a potent in vitro scavenger of peroxynitrite singlet oxygen hydroxyl radical superoxide anion and hypochlorous Acid and prevents in vivo ozone induced tyrosine nitration in lungs
Free Radical Research, 2006Co-Authors: Esau Florianosanchez, Cleva Villanueva, Omar N Medinacampos, Diana Rocha, Dolores Javier Sanchezgonzalez, Noemi Cardenasrodriguez, Jose PedrazachaverriAbstract:The antioxidant Nordihydroguaiaretic Acid (NDGA) has recently become well known as a putative anticancer drug. In this paper, it was evaluated the in vitro peroxynitrite (ONOO− ), singlet oxygen (1O2), hydroxyl radical (OH√), hydrogen peroxide (H2O2), superoxide anion and hypochlorous Acid (HOCl) scavenging capacity of NDGA. It was found that NDGA scavenges: (a) ONOO− (IC50 = 4 ± 0.94 μM) as efficiently as uric Acid; (b) 1O2 (IC50 = 151 ± 20 μM) more efficiently than dimethyl thiourea, lipoic Acid, N-acetyl-cysteine and glutathione; (c) OH√ (IC50 = 0.15 ± 0.02 μM) more efficiently than dimethyl thiourea, uric Acid, trolox, dimethyl sulfoxide and mannitol, (d) (IC50 = 15 ± 1 μM) more efficiently than N-acetyl-cysteine, glutathione, tempol and deferoxamine and (e) HOCl (IC50 = 622 ± 42 μM) as efficiently as lipoic Acid and N-acetyl-cysteine. NDGA was unable to scavenge H2O2. In an in vivo study in rats, NDGA was able to prevent ozone-induced tyrosine nitration in lungs. It is concluded that NDGA is a potent...
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Nordihydroguaiaretic Acid is a potent in vitro scavenger of peroxynitrite singlet oxygen hydroxyl radical superoxide anion and hypochlorous Acid and prevents in vivo ozone induced tyrosine nitration in lungs
Free Radical Research, 2006Co-Authors: Esau Florianosanchez, Cleva Villanueva, Omar N Medinacampos, Diana Rocha, Dolores Javier Sanchezgonzalez, Noemi Cardenasrodriguez, Jose PedrazachaverriAbstract:The antioxidant Nordihydroguaiaretic Acid (NDGA) has recently become well known as a putative anticancer drug. In this paper, it was evaluated the in vitro peroxynitrite (ONOO(-)), singlet oxygen ((1)O(2)), hydroxyl radical (OH(v)), hydrogen peroxide (H(2)O(2)), superoxide anion and hypochlorous Acid (HOCl) scavenging capacity of NDGA. It was found that NDGA scavenges: (a) ONOO(-) (IC(50) = 4 +/- 0.94 microM) as efficiently as uric Acid; (b) (1)O(2) (IC(50) = 151 +/- 20 microM) more efficiently than dimethyl thiourea, lipoic Acid, N-acetyl-cysteine and glutathione; (c) OH(v) (IC(50) = 0.15 +/- 0.02 microM) more efficiently than dimethyl thiourea, uric Acid, trolox, dimethyl sulfoxide and mannitol, (d) (IC(50) = 15 +/- 1 microM) more efficiently than N-acetyl-cysteine, glutathione, tempol and deferoxamine and (e) HOCl (IC(50) = 622 +/- 42 microM) as efficiently as lipoic Acid and N-acetyl-cysteine. NDGA was unable to scavenge H(2)O(2). In an in vivo study in rats, NDGA was able to prevent ozone-induced tyrosine nitration in lungs. It is concluded that NDGA is a potent in vitro scavenger of ONOO(-), (1)O(2), OH(v), and HOCl and is able to prevent lung tyrosine nitration in vivo.
Esau Florianosanchez - One of the best experts on this subject based on the ideXlab platform.
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Nordihydroguaiaretic Acid is a potent in vitro scavenger of peroxynitrite singlet oxygen hydroxyl radical superoxide anion and hypochlorous Acid and prevents in vivo ozone induced tyrosine nitration in lungs
Free Radical Research, 2006Co-Authors: Esau Florianosanchez, Cleva Villanueva, Omar N Medinacampos, Diana Rocha, Dolores Javier Sanchezgonzalez, Noemi Cardenasrodriguez, Jose PedrazachaverriAbstract:The antioxidant Nordihydroguaiaretic Acid (NDGA) has recently become well known as a putative anticancer drug. In this paper, it was evaluated the in vitro peroxynitrite (ONOO− ), singlet oxygen (1O2), hydroxyl radical (OH√), hydrogen peroxide (H2O2), superoxide anion and hypochlorous Acid (HOCl) scavenging capacity of NDGA. It was found that NDGA scavenges: (a) ONOO− (IC50 = 4 ± 0.94 μM) as efficiently as uric Acid; (b) 1O2 (IC50 = 151 ± 20 μM) more efficiently than dimethyl thiourea, lipoic Acid, N-acetyl-cysteine and glutathione; (c) OH√ (IC50 = 0.15 ± 0.02 μM) more efficiently than dimethyl thiourea, uric Acid, trolox, dimethyl sulfoxide and mannitol, (d) (IC50 = 15 ± 1 μM) more efficiently than N-acetyl-cysteine, glutathione, tempol and deferoxamine and (e) HOCl (IC50 = 622 ± 42 μM) as efficiently as lipoic Acid and N-acetyl-cysteine. NDGA was unable to scavenge H2O2. In an in vivo study in rats, NDGA was able to prevent ozone-induced tyrosine nitration in lungs. It is concluded that NDGA is a potent...
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Nordihydroguaiaretic Acid is a potent in vitro scavenger of peroxynitrite singlet oxygen hydroxyl radical superoxide anion and hypochlorous Acid and prevents in vivo ozone induced tyrosine nitration in lungs
Free Radical Research, 2006Co-Authors: Esau Florianosanchez, Cleva Villanueva, Omar N Medinacampos, Diana Rocha, Dolores Javier Sanchezgonzalez, Noemi Cardenasrodriguez, Jose PedrazachaverriAbstract:The antioxidant Nordihydroguaiaretic Acid (NDGA) has recently become well known as a putative anticancer drug. In this paper, it was evaluated the in vitro peroxynitrite (ONOO(-)), singlet oxygen ((1)O(2)), hydroxyl radical (OH(v)), hydrogen peroxide (H(2)O(2)), superoxide anion and hypochlorous Acid (HOCl) scavenging capacity of NDGA. It was found that NDGA scavenges: (a) ONOO(-) (IC(50) = 4 +/- 0.94 microM) as efficiently as uric Acid; (b) (1)O(2) (IC(50) = 151 +/- 20 microM) more efficiently than dimethyl thiourea, lipoic Acid, N-acetyl-cysteine and glutathione; (c) OH(v) (IC(50) = 0.15 +/- 0.02 microM) more efficiently than dimethyl thiourea, uric Acid, trolox, dimethyl sulfoxide and mannitol, (d) (IC(50) = 15 +/- 1 microM) more efficiently than N-acetyl-cysteine, glutathione, tempol and deferoxamine and (e) HOCl (IC(50) = 622 +/- 42 microM) as efficiently as lipoic Acid and N-acetyl-cysteine. NDGA was unable to scavenge H(2)O(2). In an in vivo study in rats, NDGA was able to prevent ozone-induced tyrosine nitration in lungs. It is concluded that NDGA is a potent in vitro scavenger of ONOO(-), (1)O(2), OH(v), and HOCl and is able to prevent lung tyrosine nitration in vivo.
Cleva Villanueva - One of the best experts on this subject based on the ideXlab platform.
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Nordihydroguaiaretic Acid attenuates potassium dichromate induced oxidative stress and nephrotoxicity
Food and Chemical Toxicology, 2008Co-Authors: Paola Yamcanul, Cleva Villanueva, Dolores Javier Sanchezgonzalez, Yolanda I Chirino, Claudia Maria Martinezmartinez, Cristino Cruz, Jose PedrazachaverriAbstract:Abstract Larrea tridentata also known as Creosote bush, Larrea, chaparral, greasewood or gobernadora has been used in the folk medicine for the treatment of several illnesses. The primary product that is present at high concentrations in the leaves from this plant is Nordihydroguaiaretic Acid (NDGA) which is a powerful antioxidant. On the other hand, potassium dichromate (K 2 Cr 2 O 7 )-induced nephrotoxicity is associated with oxidative stress. The aim of this work was to study the effect of NDGA on K 2 Cr 2 O 7 -induced nephrotoxicity and oxidative stress. Nephrotoxicity was induced by a single injection of K 2 Cr 2 O 7 (15 mg/Kg). A group of K 2 Cr 2 O 7 -treated rats was administered NDGA by mini osmotic pumps (17 mg/Kg/day). The results show that NDGA was able to ameliorate the structural and functional renal damage evaluated by histopathological analysis and by measuring proteinuria, urinary excretion of N -acetyl-β- d -glucosaminidase, serum creatinine, and serum glutathione peroxidase activity. In addition, immunostaining of 4-hydroxy-2-nonenal and 3-nitrotyrosine, markers of oxidative and nitrosative stress, respectively, was ameliorated by the NDGA treatment. These data strongly suggest that the antioxidant properties of NDGA are involved in its renoprotective effect in K 2 Cr 2 O 7 -treated rats.
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Nordihydroguaiaretic Acid is a potent in vitro scavenger of peroxynitrite singlet oxygen hydroxyl radical superoxide anion and hypochlorous Acid and prevents in vivo ozone induced tyrosine nitration in lungs
Free Radical Research, 2006Co-Authors: Esau Florianosanchez, Cleva Villanueva, Omar N Medinacampos, Diana Rocha, Dolores Javier Sanchezgonzalez, Noemi Cardenasrodriguez, Jose PedrazachaverriAbstract:The antioxidant Nordihydroguaiaretic Acid (NDGA) has recently become well known as a putative anticancer drug. In this paper, it was evaluated the in vitro peroxynitrite (ONOO− ), singlet oxygen (1O2), hydroxyl radical (OH√), hydrogen peroxide (H2O2), superoxide anion and hypochlorous Acid (HOCl) scavenging capacity of NDGA. It was found that NDGA scavenges: (a) ONOO− (IC50 = 4 ± 0.94 μM) as efficiently as uric Acid; (b) 1O2 (IC50 = 151 ± 20 μM) more efficiently than dimethyl thiourea, lipoic Acid, N-acetyl-cysteine and glutathione; (c) OH√ (IC50 = 0.15 ± 0.02 μM) more efficiently than dimethyl thiourea, uric Acid, trolox, dimethyl sulfoxide and mannitol, (d) (IC50 = 15 ± 1 μM) more efficiently than N-acetyl-cysteine, glutathione, tempol and deferoxamine and (e) HOCl (IC50 = 622 ± 42 μM) as efficiently as lipoic Acid and N-acetyl-cysteine. NDGA was unable to scavenge H2O2. In an in vivo study in rats, NDGA was able to prevent ozone-induced tyrosine nitration in lungs. It is concluded that NDGA is a potent...
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Nordihydroguaiaretic Acid is a potent in vitro scavenger of peroxynitrite singlet oxygen hydroxyl radical superoxide anion and hypochlorous Acid and prevents in vivo ozone induced tyrosine nitration in lungs
Free Radical Research, 2006Co-Authors: Esau Florianosanchez, Cleva Villanueva, Omar N Medinacampos, Diana Rocha, Dolores Javier Sanchezgonzalez, Noemi Cardenasrodriguez, Jose PedrazachaverriAbstract:The antioxidant Nordihydroguaiaretic Acid (NDGA) has recently become well known as a putative anticancer drug. In this paper, it was evaluated the in vitro peroxynitrite (ONOO(-)), singlet oxygen ((1)O(2)), hydroxyl radical (OH(v)), hydrogen peroxide (H(2)O(2)), superoxide anion and hypochlorous Acid (HOCl) scavenging capacity of NDGA. It was found that NDGA scavenges: (a) ONOO(-) (IC(50) = 4 +/- 0.94 microM) as efficiently as uric Acid; (b) (1)O(2) (IC(50) = 151 +/- 20 microM) more efficiently than dimethyl thiourea, lipoic Acid, N-acetyl-cysteine and glutathione; (c) OH(v) (IC(50) = 0.15 +/- 0.02 microM) more efficiently than dimethyl thiourea, uric Acid, trolox, dimethyl sulfoxide and mannitol, (d) (IC(50) = 15 +/- 1 microM) more efficiently than N-acetyl-cysteine, glutathione, tempol and deferoxamine and (e) HOCl (IC(50) = 622 +/- 42 microM) as efficiently as lipoic Acid and N-acetyl-cysteine. NDGA was unable to scavenge H(2)O(2). In an in vivo study in rats, NDGA was able to prevent ozone-induced tyrosine nitration in lungs. It is concluded that NDGA is a potent in vitro scavenger of ONOO(-), (1)O(2), OH(v), and HOCl and is able to prevent lung tyrosine nitration in vivo.
Dolores Javier Sanchezgonzalez - One of the best experts on this subject based on the ideXlab platform.
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Nordihydroguaiaretic Acid attenuates potassium dichromate induced oxidative stress and nephrotoxicity
Food and Chemical Toxicology, 2008Co-Authors: Paola Yamcanul, Cleva Villanueva, Dolores Javier Sanchezgonzalez, Yolanda I Chirino, Claudia Maria Martinezmartinez, Cristino Cruz, Jose PedrazachaverriAbstract:Abstract Larrea tridentata also known as Creosote bush, Larrea, chaparral, greasewood or gobernadora has been used in the folk medicine for the treatment of several illnesses. The primary product that is present at high concentrations in the leaves from this plant is Nordihydroguaiaretic Acid (NDGA) which is a powerful antioxidant. On the other hand, potassium dichromate (K 2 Cr 2 O 7 )-induced nephrotoxicity is associated with oxidative stress. The aim of this work was to study the effect of NDGA on K 2 Cr 2 O 7 -induced nephrotoxicity and oxidative stress. Nephrotoxicity was induced by a single injection of K 2 Cr 2 O 7 (15 mg/Kg). A group of K 2 Cr 2 O 7 -treated rats was administered NDGA by mini osmotic pumps (17 mg/Kg/day). The results show that NDGA was able to ameliorate the structural and functional renal damage evaluated by histopathological analysis and by measuring proteinuria, urinary excretion of N -acetyl-β- d -glucosaminidase, serum creatinine, and serum glutathione peroxidase activity. In addition, immunostaining of 4-hydroxy-2-nonenal and 3-nitrotyrosine, markers of oxidative and nitrosative stress, respectively, was ameliorated by the NDGA treatment. These data strongly suggest that the antioxidant properties of NDGA are involved in its renoprotective effect in K 2 Cr 2 O 7 -treated rats.
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Nordihydroguaiaretic Acid is a potent in vitro scavenger of peroxynitrite singlet oxygen hydroxyl radical superoxide anion and hypochlorous Acid and prevents in vivo ozone induced tyrosine nitration in lungs
Free Radical Research, 2006Co-Authors: Esau Florianosanchez, Cleva Villanueva, Omar N Medinacampos, Diana Rocha, Dolores Javier Sanchezgonzalez, Noemi Cardenasrodriguez, Jose PedrazachaverriAbstract:The antioxidant Nordihydroguaiaretic Acid (NDGA) has recently become well known as a putative anticancer drug. In this paper, it was evaluated the in vitro peroxynitrite (ONOO− ), singlet oxygen (1O2), hydroxyl radical (OH√), hydrogen peroxide (H2O2), superoxide anion and hypochlorous Acid (HOCl) scavenging capacity of NDGA. It was found that NDGA scavenges: (a) ONOO− (IC50 = 4 ± 0.94 μM) as efficiently as uric Acid; (b) 1O2 (IC50 = 151 ± 20 μM) more efficiently than dimethyl thiourea, lipoic Acid, N-acetyl-cysteine and glutathione; (c) OH√ (IC50 = 0.15 ± 0.02 μM) more efficiently than dimethyl thiourea, uric Acid, trolox, dimethyl sulfoxide and mannitol, (d) (IC50 = 15 ± 1 μM) more efficiently than N-acetyl-cysteine, glutathione, tempol and deferoxamine and (e) HOCl (IC50 = 622 ± 42 μM) as efficiently as lipoic Acid and N-acetyl-cysteine. NDGA was unable to scavenge H2O2. In an in vivo study in rats, NDGA was able to prevent ozone-induced tyrosine nitration in lungs. It is concluded that NDGA is a potent...
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Nordihydroguaiaretic Acid is a potent in vitro scavenger of peroxynitrite singlet oxygen hydroxyl radical superoxide anion and hypochlorous Acid and prevents in vivo ozone induced tyrosine nitration in lungs
Free Radical Research, 2006Co-Authors: Esau Florianosanchez, Cleva Villanueva, Omar N Medinacampos, Diana Rocha, Dolores Javier Sanchezgonzalez, Noemi Cardenasrodriguez, Jose PedrazachaverriAbstract:The antioxidant Nordihydroguaiaretic Acid (NDGA) has recently become well known as a putative anticancer drug. In this paper, it was evaluated the in vitro peroxynitrite (ONOO(-)), singlet oxygen ((1)O(2)), hydroxyl radical (OH(v)), hydrogen peroxide (H(2)O(2)), superoxide anion and hypochlorous Acid (HOCl) scavenging capacity of NDGA. It was found that NDGA scavenges: (a) ONOO(-) (IC(50) = 4 +/- 0.94 microM) as efficiently as uric Acid; (b) (1)O(2) (IC(50) = 151 +/- 20 microM) more efficiently than dimethyl thiourea, lipoic Acid, N-acetyl-cysteine and glutathione; (c) OH(v) (IC(50) = 0.15 +/- 0.02 microM) more efficiently than dimethyl thiourea, uric Acid, trolox, dimethyl sulfoxide and mannitol, (d) (IC(50) = 15 +/- 1 microM) more efficiently than N-acetyl-cysteine, glutathione, tempol and deferoxamine and (e) HOCl (IC(50) = 622 +/- 42 microM) as efficiently as lipoic Acid and N-acetyl-cysteine. NDGA was unable to scavenge H(2)O(2). In an in vivo study in rats, NDGA was able to prevent ozone-induced tyrosine nitration in lungs. It is concluded that NDGA is a potent in vitro scavenger of ONOO(-), (1)O(2), OH(v), and HOCl and is able to prevent lung tyrosine nitration in vivo.
