The Experts below are selected from a list of 165 Experts worldwide ranked by ideXlab platform
Ingming Chiu - One of the best experts on this subject based on the ideXlab platform.
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Cloning and interspecies comparisons of three newt (Notophthalmus viridescens) fibroblast growth factor receptor sequences
Molecular and Cellular Biochemistry, 1997Co-Authors: Matthew L Poulin, Mary Jane Botelho, Ingming ChiuAbstract:We report the nucleotide sequences of two fibroblast growth factor receptor (FGFR) cDNAs, FGFR1 and FGFR3, from the newt species Notophthalmus viridescens. These two cDNA sequences and a previously published newt FGFR cDNA, FGFR2, were used to derive the amino acid sequences which were then compared with their homologues from other species. This comparison shows that the intracellular tyrosine kinase domain is highly conserved across the species examined with the second half of the domain slightly more conserved than the first half. The 3′ portion of the carboxyl terminal tail is not very highly conserved. The comparison of the extracellular portion of FGFR2 shows a high degree of conservation among the Ig-like domains and a low degree of conservation in the region that links the third Ig-like domain with the transmembrane domain. (Mol Cell Biochem 175: 11–19, 1997)
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re programming of expression of the kgfr and bek variants of fibroblast growth factor receptor 2 during limb regeneration in newts Notophthalmus viridescens
Developmental Dynamics, 1995Co-Authors: Matthew L Poulin, Ingming ChiuAbstract:We have previously shown, by in situ hybridization, that fibroblast growth factor receptor 2 (FGFRB) is present in the basal layer of wound epithelium during limb regeneration in newts (Notophthalmus viridescens). In contrast, FGFRl expression is observed throughout the blastema mesenchyme but is distinctly absent from the wound epithelium (Poulin et al. (1993) Development 1193534361). Sequence analysis re- vealed that we have isolated both the KGFR and bek variants of FGFR2. These two variants differ only in the second half of the last of their three (or two) Ig-like domains. In this report, we show the expression patterns of FGFRB variants during limb regeneration by in situ hybridization. During the pre-blastema stages of regeneration, FGFRB expression was observed in the basal layer of the wound epithelium and in the cells of the perios- bum. The wound epithelial hybridization was ob-
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nucleotide sequences of two newt Notophthalmus viridescens fibroblast growth factor receptor 2 variants
Biochimica et Biophysica Acta, 1994Co-Authors: Matthew L Poulin, Ingming ChiuAbstract:Abstract The FGF receptor tyrosine kinase family consists of four members. We report the sequence of two newt (Notophthalmus viridescens) FGFR2 cDNAs which were isolated from a forelimb blastema cDNA library and represent the newt cognates of two different isoforms of FGFR2, one homologous to bek the other to the KGFR.
Matthew L Poulin - One of the best experts on this subject based on the ideXlab platform.
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Cloning and interspecies comparisons of three newt (Notophthalmus viridescens) fibroblast growth factor receptor sequences
Molecular and Cellular Biochemistry, 1997Co-Authors: Matthew L Poulin, Mary Jane Botelho, Ingming ChiuAbstract:We report the nucleotide sequences of two fibroblast growth factor receptor (FGFR) cDNAs, FGFR1 and FGFR3, from the newt species Notophthalmus viridescens. These two cDNA sequences and a previously published newt FGFR cDNA, FGFR2, were used to derive the amino acid sequences which were then compared with their homologues from other species. This comparison shows that the intracellular tyrosine kinase domain is highly conserved across the species examined with the second half of the domain slightly more conserved than the first half. The 3′ portion of the carboxyl terminal tail is not very highly conserved. The comparison of the extracellular portion of FGFR2 shows a high degree of conservation among the Ig-like domains and a low degree of conservation in the region that links the third Ig-like domain with the transmembrane domain. (Mol Cell Biochem 175: 11–19, 1997)
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re programming of expression of the kgfr and bek variants of fibroblast growth factor receptor 2 during limb regeneration in newts Notophthalmus viridescens
Developmental Dynamics, 1995Co-Authors: Matthew L Poulin, Ingming ChiuAbstract:We have previously shown, by in situ hybridization, that fibroblast growth factor receptor 2 (FGFRB) is present in the basal layer of wound epithelium during limb regeneration in newts (Notophthalmus viridescens). In contrast, FGFRl expression is observed throughout the blastema mesenchyme but is distinctly absent from the wound epithelium (Poulin et al. (1993) Development 1193534361). Sequence analysis re- vealed that we have isolated both the KGFR and bek variants of FGFR2. These two variants differ only in the second half of the last of their three (or two) Ig-like domains. In this report, we show the expression patterns of FGFRB variants during limb regeneration by in situ hybridization. During the pre-blastema stages of regeneration, FGFRB expression was observed in the basal layer of the wound epithelium and in the cells of the perios- bum. The wound epithelial hybridization was ob-
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nucleotide sequences of two newt Notophthalmus viridescens fibroblast growth factor receptor 2 variants
Biochimica et Biophysica Acta, 1994Co-Authors: Matthew L Poulin, Ingming ChiuAbstract:Abstract The FGF receptor tyrosine kinase family consists of four members. We report the sequence of two newt (Notophthalmus viridescens) FGFR2 cDNAs which were isolated from a forelimb blastema cDNA library and represent the newt cognates of two different isoforms of FGFR2, one homologous to bek the other to the KGFR.
Dietrich Mebs - One of the best experts on this subject based on the ideXlab platform.
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tetrodotoxin does not protect red spotted newts Notophthalmus viridescens from intestinal parasites
Toxicon, 2012Co-Authors: Dietrich Mebs, Mari Yotsuyamashita, Hanns M Seitz, Osamu ArakawaAbstract:Abstract Tetrodotoxin (TTX) and its analogue 6- epi TTX had been detected in the red-spotted newt, Notophthalmus viridescens . Thirty specimens of a population from Pennsylvania, USA were histologically examined for the presence of intestinal parasites. More than 50% were found to be infected with nematodes (Trichocephalidae), trematodes or cestodes (Pseudophyllidae). The mean values of TTX and 6- epi TTX in parasitized and in non-parasitized newts were not significantly different. Using a monoclonal antibody-based immunoenzymatic technique, TTX was localized in the intestinal tissue as well as in the parasites indicating that they accumulate the toxin and suggesting that TTX and 6- epi TTX are not providing protection from parasites to the newts.
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tissue distribution of tetrodotoxin in the red spotted newt Notophthalmus viridescens
Toxicon, 2010Co-Authors: Dietrich Mebs, Osamu Arakawa, Mari YotsuyamashitaAbstract:High levels of tetrodotoxin (TTX) and of its analogue 6-epi-tetrodotoxin have been detected in skin and liver extracts of the red-spotted newt Notophthalmus viridescens assayed by a post-column fluorescent-HPLC system. Using a monoclonal antibody-based immunoenzymatic technique, TTX was localized in the granular glands of the epidermis as well as in the muscle layer between the muscle fibres, but also in most organs suggesting that TTX is stored in the whole body of the newt and is secreted by skin glands.
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occurrence of 11 oxotetrodotoxin in the red spotted newt Notophthalmus viridescens and further studies on the levels of tetrodotoxin and its analogues in the newt s efts
Toxicon, 2003Co-Authors: Mari Yotsuyamashita, Dietrich MebsAbstract:An analogue of tetrodotoxin (TTX), 11-oxoTTX, was semi-purified from the red-spotted newt, Notophthalmus viridescens, and identified by ESI-MS and 1H NMR spectra. The levels of TTX, 11-oxoTTX and 6-epiTTX in early stage of development (efts) of this newt were investigated by a post-column fluorescent-HPLC system, and compared with those of adult newts. The level of 11-oxoTTX in both of adults and efts were remarkably high, almost close to those of TTX, while 6-epiTTX was a minor component in both stages. The level of 6-epiTTX in efts (1.8±1.3 μg/g, SD, n=10) was significantly larger than that in adults (0.51±0.26 μg/g, SD, n=12) (p 0.05).
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the levels of tetrodotoxin and its analogue 6 epitetrodotoxin in the red spotted newt Notophthalmus viridescens
Toxicon, 2001Co-Authors: Mari Yotsuyamashita, Dietrich MebsAbstract:Abstract Tetrodotoxin (TTX) and its analogue 6- epi TTX were detected in 11–12 specimens of the red-spotted newt, Notophthalmus viridescens , by a post-column fluorescent-HPLC system and by LC/MS in selected ion monitoring mode. TTX levels varied considerably among individuals from low (less than 0.15 μg TTX/g newt) to high concentrations (23.5 μg TTX/g newt), while 6- epi TTX was found to be a minor constituent in all specimens.
Mari Yotsuyamashita - One of the best experts on this subject based on the ideXlab platform.
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tetrodotoxin does not protect red spotted newts Notophthalmus viridescens from intestinal parasites
Toxicon, 2012Co-Authors: Dietrich Mebs, Mari Yotsuyamashita, Hanns M Seitz, Osamu ArakawaAbstract:Abstract Tetrodotoxin (TTX) and its analogue 6- epi TTX had been detected in the red-spotted newt, Notophthalmus viridescens . Thirty specimens of a population from Pennsylvania, USA were histologically examined for the presence of intestinal parasites. More than 50% were found to be infected with nematodes (Trichocephalidae), trematodes or cestodes (Pseudophyllidae). The mean values of TTX and 6- epi TTX in parasitized and in non-parasitized newts were not significantly different. Using a monoclonal antibody-based immunoenzymatic technique, TTX was localized in the intestinal tissue as well as in the parasites indicating that they accumulate the toxin and suggesting that TTX and 6- epi TTX are not providing protection from parasites to the newts.
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tissue distribution of tetrodotoxin in the red spotted newt Notophthalmus viridescens
Toxicon, 2010Co-Authors: Dietrich Mebs, Osamu Arakawa, Mari YotsuyamashitaAbstract:High levels of tetrodotoxin (TTX) and of its analogue 6-epi-tetrodotoxin have been detected in skin and liver extracts of the red-spotted newt Notophthalmus viridescens assayed by a post-column fluorescent-HPLC system. Using a monoclonal antibody-based immunoenzymatic technique, TTX was localized in the granular glands of the epidermis as well as in the muscle layer between the muscle fibres, but also in most organs suggesting that TTX is stored in the whole body of the newt and is secreted by skin glands.
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occurrence of 11 oxotetrodotoxin in the red spotted newt Notophthalmus viridescens and further studies on the levels of tetrodotoxin and its analogues in the newt s efts
Toxicon, 2003Co-Authors: Mari Yotsuyamashita, Dietrich MebsAbstract:An analogue of tetrodotoxin (TTX), 11-oxoTTX, was semi-purified from the red-spotted newt, Notophthalmus viridescens, and identified by ESI-MS and 1H NMR spectra. The levels of TTX, 11-oxoTTX and 6-epiTTX in early stage of development (efts) of this newt were investigated by a post-column fluorescent-HPLC system, and compared with those of adult newts. The level of 11-oxoTTX in both of adults and efts were remarkably high, almost close to those of TTX, while 6-epiTTX was a minor component in both stages. The level of 6-epiTTX in efts (1.8±1.3 μg/g, SD, n=10) was significantly larger than that in adults (0.51±0.26 μg/g, SD, n=12) (p 0.05).
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the levels of tetrodotoxin and its analogue 6 epitetrodotoxin in the red spotted newt Notophthalmus viridescens
Toxicon, 2001Co-Authors: Mari Yotsuyamashita, Dietrich MebsAbstract:Abstract Tetrodotoxin (TTX) and its analogue 6- epi TTX were detected in 11–12 specimens of the red-spotted newt, Notophthalmus viridescens , by a post-column fluorescent-HPLC system and by LC/MS in selected ion monitoring mode. TTX levels varied considerably among individuals from low (less than 0.15 μg TTX/g newt) to high concentrations (23.5 μg TTX/g newt), while 6- epi TTX was found to be a minor constituent in all specimens.
Rickard Sandberg - One of the best experts on this subject based on the ideXlab platform.
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a reference transcriptome and inferred proteome for the salamander Notophthalmus viridescens
Experimental Cell Research, 2013Co-Authors: Ilgar Abdullayev, Matthew Kirkham, Asa K Bjorklund, Andras Simon, Rickard SandbergAbstract:Abstract Salamanders have a remarkable capacity to regenerate complex tissues, such as limbs and brain, and are therefore an important comparative model system for regenerative medicine. Despite these unique properties among adult vertebrates, the genomic information for amphibians in general, and salamanders in particular, is scarce. Here, we used massive parallel sequencing to reconstruct a de novo reference transcriptome of the red spotted newt ( Notophthalmus viridescens ) containing 118,893 transcripts with a N50 length of 2016 nts. Comparisons to other vertebrates revealed a newt transcriptome that is comparable in size and characteristics to well-annotated vertebrate transcriptomes. Identification of putative open reading frames (ORFs) enabled us to infer a comprehensive proteome, including the annotation of 19,903 newt proteins. We used the identified domain architectures (DAs) to assign ORFs phylogenetic positions, which also revealed putative salamander specific proteins. The reference transcriptome and inferred proteome of the red spotted newt will facilitate the use of systematic genomic technologies for regeneration studies in salamanders and enable evolutionary analyses of vertebrate regeneration at the molecular level.
