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Jinxin Wang - One of the best experts on this subject based on the ideXlab platform.

  • acetolactate synthase overexpression in mesosulfuron methyl resistant shortawn foxtail alopecurus aequalis sobol reference gene selection and herbicide target gene expression analysis
    Journal of Agricultural and Food Chemistry, 2018
    Co-Authors: Ning Zhao, Yanyan Yan, Hengzhi Wang, Shuang Bai, Qian Wang, Weitang Liu, Jinxin Wang
    Abstract:

    Severe infestations of shortawn foxtail ( Alopecurus aequalis Sobol.), a Noxious Weed in wheat and canola cropping systems in China, remain standing even after the application of the herbicides, fenoxaprop- P-ethyl and mesosulfuron-methyl. Analysis of gene expression in Weed plants subjected to herbicide treatment is a key step toward more mechanistic studies. Since such an analysis often involves quantitative real-time PCR (qRT-PCR), endogenous reference genes with stable expression are required. Herein, we identified specific gene sets, suitable as references for qRT-PCR data normalization in A. aequalis plants under different experimental conditions, using geNorm, NormFinder, BestKeeper, and RefFinder software. Additionally, the reliability of reference genes was verified by analyzing the expression of genes encoding two major herbicide target enzymes: acetyl-CoA carboxylase (ACCase) and acetolactate synthase (ALS). Furthermore, functional ALS gene amplification was likely present in resistant plants, although it may make no contribution to the resistant phenotypes.

  • Acetolactate Synthase Overexpression in Mesosulfuron-Methyl-Resistant Shortawn Foxtail (Alopecurus aequalis Sobol.): Reference Gene Selection and Herbicide Target Gene Expression Analysis
    2018
    Co-Authors: Ning Zhao, Yanyan Yan, Hengzhi Wang, Shuang Bai, Qian Wang, Weitang Liu, Jinxin Wang
    Abstract:

    Severe infestations of shortawn foxtail (Alopecurus aequalis Sobol.), a Noxious Weed in wheat and canola cropping systems in China, remain standing even after the application of the herbicides, fenoxaprop-P-ethyl and mesosulfuron-methyl. Analysis of gene expression in Weed plants subjected to herbicide treatment is a key step toward more mechanistic studies. Since such an analysis often involves quantitative real-time PCR (qRT-PCR), endogenous reference genes with stable expression are required. Herein, we identified specific gene sets, suitable as references for qRT-PCR data normalization in A. aequalis plants under different experimental conditions, using geNorm, NormFinder, BestKeeper, and RefFinder software. Additionally, the reliability of reference genes was verified by analyzing the expression of genes encoding two major herbicide target enzymes: acetyl-CoA carboxylase (ACCase) and acetolactate synthase (ALS). Furthermore, functional ALS gene amplification was likely present in resistant plants, although it may make no contribution to the resistant phenotypes

Ning Zhao - One of the best experts on this subject based on the ideXlab platform.

  • acetolactate synthase overexpression in mesosulfuron methyl resistant shortawn foxtail alopecurus aequalis sobol reference gene selection and herbicide target gene expression analysis
    Journal of Agricultural and Food Chemistry, 2018
    Co-Authors: Ning Zhao, Yanyan Yan, Hengzhi Wang, Shuang Bai, Qian Wang, Weitang Liu, Jinxin Wang
    Abstract:

    Severe infestations of shortawn foxtail ( Alopecurus aequalis Sobol.), a Noxious Weed in wheat and canola cropping systems in China, remain standing even after the application of the herbicides, fenoxaprop- P-ethyl and mesosulfuron-methyl. Analysis of gene expression in Weed plants subjected to herbicide treatment is a key step toward more mechanistic studies. Since such an analysis often involves quantitative real-time PCR (qRT-PCR), endogenous reference genes with stable expression are required. Herein, we identified specific gene sets, suitable as references for qRT-PCR data normalization in A. aequalis plants under different experimental conditions, using geNorm, NormFinder, BestKeeper, and RefFinder software. Additionally, the reliability of reference genes was verified by analyzing the expression of genes encoding two major herbicide target enzymes: acetyl-CoA carboxylase (ACCase) and acetolactate synthase (ALS). Furthermore, functional ALS gene amplification was likely present in resistant plants, although it may make no contribution to the resistant phenotypes.

  • Acetolactate Synthase Overexpression in Mesosulfuron-Methyl-Resistant Shortawn Foxtail (Alopecurus aequalis Sobol.): Reference Gene Selection and Herbicide Target Gene Expression Analysis
    2018
    Co-Authors: Ning Zhao, Yanyan Yan, Hengzhi Wang, Shuang Bai, Qian Wang, Weitang Liu, Jinxin Wang
    Abstract:

    Severe infestations of shortawn foxtail (Alopecurus aequalis Sobol.), a Noxious Weed in wheat and canola cropping systems in China, remain standing even after the application of the herbicides, fenoxaprop-P-ethyl and mesosulfuron-methyl. Analysis of gene expression in Weed plants subjected to herbicide treatment is a key step toward more mechanistic studies. Since such an analysis often involves quantitative real-time PCR (qRT-PCR), endogenous reference genes with stable expression are required. Herein, we identified specific gene sets, suitable as references for qRT-PCR data normalization in A. aequalis plants under different experimental conditions, using geNorm, NormFinder, BestKeeper, and RefFinder software. Additionally, the reliability of reference genes was verified by analyzing the expression of genes encoding two major herbicide target enzymes: acetyl-CoA carboxylase (ACCase) and acetolactate synthase (ALS). Furthermore, functional ALS gene amplification was likely present in resistant plants, although it may make no contribution to the resistant phenotypes

Tracy M Sterling - One of the best experts on this subject based on the ideXlab platform.

  • Inheritance of Resistance to Clopyralid and Picloram in Yellow Starthistle (Centaurea solstitialis L.) Is Controlled by a Single Nuclear Recessive Gene
    2015
    Co-Authors: Robert P Sabba, Norman K Lownds, I. M. Ray, Tracy M Sterling
    Abstract:

    The Noxious Weed yellow starthistle (Centaurea solstitialis L.) can be controlled effectively at the seedling stage with foliar application of the auxinic herbicides picloram or clopyralid. Although resistance to these herbicides is rare, a yellow starthistle biotype resistant to picloram and cross-resistant to clopyralid was observed in 1989 near Dayton, WA, in a pasture that had been subjected to intensive picloram selective pressure. Our objective was to determine the mode of inheritance for this resistance trait. Transmission of the resistant phenotype was monitored in reciprocal F1 crosses between susceptible (SCI) and resistant (RDW) plants, their testcross and pseudo-F2 progeny. Progeny from all crosses, as well as RDW and SCI seedlings of original populations, were sprayed with picloram or clopyralid to distinguish between susceptible and resistant individuals. All F1 progeny wer

  • inheritance of resistance to clopyralid and picloram in yellow starthistle centaurea solstitialis l is controlled by a single nuclear recessive gene
    Journal of Heredity, 2003
    Co-Authors: Robert P Sabba, Norman K Lownds, Tracy M Sterling
    Abstract:

    The Noxious Weed yellow starthistle (Centaurea solstitialis L.) can be controlled effectively at the seedling stage with foliar application of the auxinic herbicides picloram or clopyralid. Although resistance to these herbicides is rare, a yellow starthistle biotype resistant to picloram and cross-resistant to clopyralid was observed in 1989 near Dayton, WA, in a pasture that had been subjected to intensive picloram selective pressure. Our objective was to determine the mode of inheritance for this resistance trait. Transmission of the resistant phenotype was monitored in reciprocal F1 crosses between susceptible (SCI) and resistant (RDW) plants, their testcross and pseudo-F2 progeny. Progeny from all crosses, as well as RDW and SCI seedlings of original populations, were sprayed with picloram or clopyralid to distinguish between susceptible and resistant individuals. All F1 progeny were susceptible to both herbicides, indicating that the resistance trait was of nuclear origin and recessive in nature. Segregation of the resistant phenotype among pseudo-F2 and testcross progeny of F1 genotypes demonstrated monofactorial inheritance (P ..25)for resistance to both herbicides. The conclusion that resistanceis conferred by a single recessive allele is consistent with the observation that no other picloramresistant yellow starthistle populations have been identified in the area since picloram selection pressure was abated. Yellow starthistle (Centaurea solstitialis L.) is a highly competitive diploid winter annual of Eurasian origin that is advancing steadily on western rangelands of the United States. This Noxious Weed can be controlled effectively at the seedling stage by foliar application of the pyridinecarboxylic acid herbicides picloram or clopyralid. These compounds are

Yanyan Yan - One of the best experts on this subject based on the ideXlab platform.

  • acetolactate synthase overexpression in mesosulfuron methyl resistant shortawn foxtail alopecurus aequalis sobol reference gene selection and herbicide target gene expression analysis
    Journal of Agricultural and Food Chemistry, 2018
    Co-Authors: Ning Zhao, Yanyan Yan, Hengzhi Wang, Shuang Bai, Qian Wang, Weitang Liu, Jinxin Wang
    Abstract:

    Severe infestations of shortawn foxtail ( Alopecurus aequalis Sobol.), a Noxious Weed in wheat and canola cropping systems in China, remain standing even after the application of the herbicides, fenoxaprop- P-ethyl and mesosulfuron-methyl. Analysis of gene expression in Weed plants subjected to herbicide treatment is a key step toward more mechanistic studies. Since such an analysis often involves quantitative real-time PCR (qRT-PCR), endogenous reference genes with stable expression are required. Herein, we identified specific gene sets, suitable as references for qRT-PCR data normalization in A. aequalis plants under different experimental conditions, using geNorm, NormFinder, BestKeeper, and RefFinder software. Additionally, the reliability of reference genes was verified by analyzing the expression of genes encoding two major herbicide target enzymes: acetyl-CoA carboxylase (ACCase) and acetolactate synthase (ALS). Furthermore, functional ALS gene amplification was likely present in resistant plants, although it may make no contribution to the resistant phenotypes.

  • Acetolactate Synthase Overexpression in Mesosulfuron-Methyl-Resistant Shortawn Foxtail (Alopecurus aequalis Sobol.): Reference Gene Selection and Herbicide Target Gene Expression Analysis
    2018
    Co-Authors: Ning Zhao, Yanyan Yan, Hengzhi Wang, Shuang Bai, Qian Wang, Weitang Liu, Jinxin Wang
    Abstract:

    Severe infestations of shortawn foxtail (Alopecurus aequalis Sobol.), a Noxious Weed in wheat and canola cropping systems in China, remain standing even after the application of the herbicides, fenoxaprop-P-ethyl and mesosulfuron-methyl. Analysis of gene expression in Weed plants subjected to herbicide treatment is a key step toward more mechanistic studies. Since such an analysis often involves quantitative real-time PCR (qRT-PCR), endogenous reference genes with stable expression are required. Herein, we identified specific gene sets, suitable as references for qRT-PCR data normalization in A. aequalis plants under different experimental conditions, using geNorm, NormFinder, BestKeeper, and RefFinder software. Additionally, the reliability of reference genes was verified by analyzing the expression of genes encoding two major herbicide target enzymes: acetyl-CoA carboxylase (ACCase) and acetolactate synthase (ALS). Furthermore, functional ALS gene amplification was likely present in resistant plants, although it may make no contribution to the resistant phenotypes

Hengzhi Wang - One of the best experts on this subject based on the ideXlab platform.

  • acetolactate synthase overexpression in mesosulfuron methyl resistant shortawn foxtail alopecurus aequalis sobol reference gene selection and herbicide target gene expression analysis
    Journal of Agricultural and Food Chemistry, 2018
    Co-Authors: Ning Zhao, Yanyan Yan, Hengzhi Wang, Shuang Bai, Qian Wang, Weitang Liu, Jinxin Wang
    Abstract:

    Severe infestations of shortawn foxtail ( Alopecurus aequalis Sobol.), a Noxious Weed in wheat and canola cropping systems in China, remain standing even after the application of the herbicides, fenoxaprop- P-ethyl and mesosulfuron-methyl. Analysis of gene expression in Weed plants subjected to herbicide treatment is a key step toward more mechanistic studies. Since such an analysis often involves quantitative real-time PCR (qRT-PCR), endogenous reference genes with stable expression are required. Herein, we identified specific gene sets, suitable as references for qRT-PCR data normalization in A. aequalis plants under different experimental conditions, using geNorm, NormFinder, BestKeeper, and RefFinder software. Additionally, the reliability of reference genes was verified by analyzing the expression of genes encoding two major herbicide target enzymes: acetyl-CoA carboxylase (ACCase) and acetolactate synthase (ALS). Furthermore, functional ALS gene amplification was likely present in resistant plants, although it may make no contribution to the resistant phenotypes.

  • Acetolactate Synthase Overexpression in Mesosulfuron-Methyl-Resistant Shortawn Foxtail (Alopecurus aequalis Sobol.): Reference Gene Selection and Herbicide Target Gene Expression Analysis
    2018
    Co-Authors: Ning Zhao, Yanyan Yan, Hengzhi Wang, Shuang Bai, Qian Wang, Weitang Liu, Jinxin Wang
    Abstract:

    Severe infestations of shortawn foxtail (Alopecurus aequalis Sobol.), a Noxious Weed in wheat and canola cropping systems in China, remain standing even after the application of the herbicides, fenoxaprop-P-ethyl and mesosulfuron-methyl. Analysis of gene expression in Weed plants subjected to herbicide treatment is a key step toward more mechanistic studies. Since such an analysis often involves quantitative real-time PCR (qRT-PCR), endogenous reference genes with stable expression are required. Herein, we identified specific gene sets, suitable as references for qRT-PCR data normalization in A. aequalis plants under different experimental conditions, using geNorm, NormFinder, BestKeeper, and RefFinder software. Additionally, the reliability of reference genes was verified by analyzing the expression of genes encoding two major herbicide target enzymes: acetyl-CoA carboxylase (ACCase) and acetolactate synthase (ALS). Furthermore, functional ALS gene amplification was likely present in resistant plants, although it may make no contribution to the resistant phenotypes

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