The Experts below are selected from a list of 303 Experts worldwide ranked by ideXlab platform
Bang Liu - One of the best experts on this subject based on the ideXlab platform.
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a multiplex real time pcr approach for identification and quantification of sheep goat fox and murine fractions in meats using Nuclear DNA sequences
Food Control, 2021Co-Authors: Wenjun Wang, Xinyi Wang, Tiean Wei, Qingde Zhang, Xiang Zhou, Bang LiuAbstract:Abstract Adulterating undeclared animal components into meats may cause health problems. In this study, Nuclear DNA sequences for specific detection of sheep/goat, fox and murine (mouse, rat and hamster) were selected to design primers and probes for determination of mutton adulteration. The inter-specific specificity and intra-specific conservation were strictly evaluated in different individuals of 19 animal species. These three Nuclear DNA sequences were confirmed to be fixed copy sequences by relative quantitative PCR. Furthermore, a multiplex qualitative and quantitative PCR system with sensitivity of 0.05 ng was developed to efficiently discriminate sheep/goat (237 bp), fox (211 bp) and murine (160 bp) in one reaction. Finally, several mixed DNA samples of goat, fox and rat were quantitatively analyzed by multiplex real-time PCR method, showing good accuracy (R.E. from 1.64% to 18.43%) and precision (R.S.D. from 0.51% to 12.70%). The method developed in this study will provide a powerful technology to combat meat adulteration and protect consumer health.
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a multiplex real time pcr approach for identification and quantification of sheep goat fox and murine fractions in meats using Nuclear DNA sequences
Food Control, 2021Co-Authors: Wenjun Wang, Xinyi Wang, Tiean Wei, Qingde Zhang, Xiang Zhou, Bang LiuAbstract:Abstract Adulterating undeclared animal components into meats may cause health problems. In this study, Nuclear DNA sequences for specific detection of sheep/goat, fox and murine (mouse, rat and hamster) were selected to design primers and probes for determination of mutton adulteration. The inter-specific specificity and intra-specific conservation were strictly evaluated in 19 different animal species and breeds, respectively. These three Nuclear DNA sequences were confirmed to be fixed copy sequences by relative quantitative PCR. Furthermore, a multiplex qualitative and quantitative PCR system with sensitivity of 0.05 ng was developed to efficiently discriminate sheep/goat (237 bp), fox (211 bp) and murine (160 bp) in one reaction. Finally, several mixed DNA samples of goat, fox and rat were quantitatively analyzed by multiplex real-time PCR method, showing good accuracy (R.E. from 1.64% to 18.43%) and precision (R.S.D. from 0.51% to 12.70%). The method developed in this study will provide a powerful technology to combat meat adulteration and protect consumer health.
Wenjun Wang - One of the best experts on this subject based on the ideXlab platform.
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a multiplex real time pcr approach for identification and quantification of sheep goat fox and murine fractions in meats using Nuclear DNA sequences
Food Control, 2021Co-Authors: Wenjun Wang, Xinyi Wang, Tiean Wei, Qingde Zhang, Xiang Zhou, Bang LiuAbstract:Abstract Adulterating undeclared animal components into meats may cause health problems. In this study, Nuclear DNA sequences for specific detection of sheep/goat, fox and murine (mouse, rat and hamster) were selected to design primers and probes for determination of mutton adulteration. The inter-specific specificity and intra-specific conservation were strictly evaluated in different individuals of 19 animal species. These three Nuclear DNA sequences were confirmed to be fixed copy sequences by relative quantitative PCR. Furthermore, a multiplex qualitative and quantitative PCR system with sensitivity of 0.05 ng was developed to efficiently discriminate sheep/goat (237 bp), fox (211 bp) and murine (160 bp) in one reaction. Finally, several mixed DNA samples of goat, fox and rat were quantitatively analyzed by multiplex real-time PCR method, showing good accuracy (R.E. from 1.64% to 18.43%) and precision (R.S.D. from 0.51% to 12.70%). The method developed in this study will provide a powerful technology to combat meat adulteration and protect consumer health.
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a multiplex real time pcr approach for identification and quantification of sheep goat fox and murine fractions in meats using Nuclear DNA sequences
Food Control, 2021Co-Authors: Wenjun Wang, Xinyi Wang, Tiean Wei, Qingde Zhang, Xiang Zhou, Bang LiuAbstract:Abstract Adulterating undeclared animal components into meats may cause health problems. In this study, Nuclear DNA sequences for specific detection of sheep/goat, fox and murine (mouse, rat and hamster) were selected to design primers and probes for determination of mutton adulteration. The inter-specific specificity and intra-specific conservation were strictly evaluated in 19 different animal species and breeds, respectively. These three Nuclear DNA sequences were confirmed to be fixed copy sequences by relative quantitative PCR. Furthermore, a multiplex qualitative and quantitative PCR system with sensitivity of 0.05 ng was developed to efficiently discriminate sheep/goat (237 bp), fox (211 bp) and murine (160 bp) in one reaction. Finally, several mixed DNA samples of goat, fox and rat were quantitatively analyzed by multiplex real-time PCR method, showing good accuracy (R.E. from 1.64% to 18.43%) and precision (R.S.D. from 0.51% to 12.70%). The method developed in this study will provide a powerful technology to combat meat adulteration and protect consumer health.
Xinyi Wang - One of the best experts on this subject based on the ideXlab platform.
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a multiplex real time pcr approach for identification and quantification of sheep goat fox and murine fractions in meats using Nuclear DNA sequences
Food Control, 2021Co-Authors: Wenjun Wang, Xinyi Wang, Tiean Wei, Qingde Zhang, Xiang Zhou, Bang LiuAbstract:Abstract Adulterating undeclared animal components into meats may cause health problems. In this study, Nuclear DNA sequences for specific detection of sheep/goat, fox and murine (mouse, rat and hamster) were selected to design primers and probes for determination of mutton adulteration. The inter-specific specificity and intra-specific conservation were strictly evaluated in different individuals of 19 animal species. These three Nuclear DNA sequences were confirmed to be fixed copy sequences by relative quantitative PCR. Furthermore, a multiplex qualitative and quantitative PCR system with sensitivity of 0.05 ng was developed to efficiently discriminate sheep/goat (237 bp), fox (211 bp) and murine (160 bp) in one reaction. Finally, several mixed DNA samples of goat, fox and rat were quantitatively analyzed by multiplex real-time PCR method, showing good accuracy (R.E. from 1.64% to 18.43%) and precision (R.S.D. from 0.51% to 12.70%). The method developed in this study will provide a powerful technology to combat meat adulteration and protect consumer health.
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a multiplex real time pcr approach for identification and quantification of sheep goat fox and murine fractions in meats using Nuclear DNA sequences
Food Control, 2021Co-Authors: Wenjun Wang, Xinyi Wang, Tiean Wei, Qingde Zhang, Xiang Zhou, Bang LiuAbstract:Abstract Adulterating undeclared animal components into meats may cause health problems. In this study, Nuclear DNA sequences for specific detection of sheep/goat, fox and murine (mouse, rat and hamster) were selected to design primers and probes for determination of mutton adulteration. The inter-specific specificity and intra-specific conservation were strictly evaluated in 19 different animal species and breeds, respectively. These three Nuclear DNA sequences were confirmed to be fixed copy sequences by relative quantitative PCR. Furthermore, a multiplex qualitative and quantitative PCR system with sensitivity of 0.05 ng was developed to efficiently discriminate sheep/goat (237 bp), fox (211 bp) and murine (160 bp) in one reaction. Finally, several mixed DNA samples of goat, fox and rat were quantitatively analyzed by multiplex real-time PCR method, showing good accuracy (R.E. from 1.64% to 18.43%) and precision (R.S.D. from 0.51% to 12.70%). The method developed in this study will provide a powerful technology to combat meat adulteration and protect consumer health.
Tiean Wei - One of the best experts on this subject based on the ideXlab platform.
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a multiplex real time pcr approach for identification and quantification of sheep goat fox and murine fractions in meats using Nuclear DNA sequences
Food Control, 2021Co-Authors: Wenjun Wang, Xinyi Wang, Tiean Wei, Qingde Zhang, Xiang Zhou, Bang LiuAbstract:Abstract Adulterating undeclared animal components into meats may cause health problems. In this study, Nuclear DNA sequences for specific detection of sheep/goat, fox and murine (mouse, rat and hamster) were selected to design primers and probes for determination of mutton adulteration. The inter-specific specificity and intra-specific conservation were strictly evaluated in different individuals of 19 animal species. These three Nuclear DNA sequences were confirmed to be fixed copy sequences by relative quantitative PCR. Furthermore, a multiplex qualitative and quantitative PCR system with sensitivity of 0.05 ng was developed to efficiently discriminate sheep/goat (237 bp), fox (211 bp) and murine (160 bp) in one reaction. Finally, several mixed DNA samples of goat, fox and rat were quantitatively analyzed by multiplex real-time PCR method, showing good accuracy (R.E. from 1.64% to 18.43%) and precision (R.S.D. from 0.51% to 12.70%). The method developed in this study will provide a powerful technology to combat meat adulteration and protect consumer health.
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a multiplex real time pcr approach for identification and quantification of sheep goat fox and murine fractions in meats using Nuclear DNA sequences
Food Control, 2021Co-Authors: Wenjun Wang, Xinyi Wang, Tiean Wei, Qingde Zhang, Xiang Zhou, Bang LiuAbstract:Abstract Adulterating undeclared animal components into meats may cause health problems. In this study, Nuclear DNA sequences for specific detection of sheep/goat, fox and murine (mouse, rat and hamster) were selected to design primers and probes for determination of mutton adulteration. The inter-specific specificity and intra-specific conservation were strictly evaluated in 19 different animal species and breeds, respectively. These three Nuclear DNA sequences were confirmed to be fixed copy sequences by relative quantitative PCR. Furthermore, a multiplex qualitative and quantitative PCR system with sensitivity of 0.05 ng was developed to efficiently discriminate sheep/goat (237 bp), fox (211 bp) and murine (160 bp) in one reaction. Finally, several mixed DNA samples of goat, fox and rat were quantitatively analyzed by multiplex real-time PCR method, showing good accuracy (R.E. from 1.64% to 18.43%) and precision (R.S.D. from 0.51% to 12.70%). The method developed in this study will provide a powerful technology to combat meat adulteration and protect consumer health.
Qingde Zhang - One of the best experts on this subject based on the ideXlab platform.
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a multiplex real time pcr approach for identification and quantification of sheep goat fox and murine fractions in meats using Nuclear DNA sequences
Food Control, 2021Co-Authors: Wenjun Wang, Xinyi Wang, Tiean Wei, Qingde Zhang, Xiang Zhou, Bang LiuAbstract:Abstract Adulterating undeclared animal components into meats may cause health problems. In this study, Nuclear DNA sequences for specific detection of sheep/goat, fox and murine (mouse, rat and hamster) were selected to design primers and probes for determination of mutton adulteration. The inter-specific specificity and intra-specific conservation were strictly evaluated in different individuals of 19 animal species. These three Nuclear DNA sequences were confirmed to be fixed copy sequences by relative quantitative PCR. Furthermore, a multiplex qualitative and quantitative PCR system with sensitivity of 0.05 ng was developed to efficiently discriminate sheep/goat (237 bp), fox (211 bp) and murine (160 bp) in one reaction. Finally, several mixed DNA samples of goat, fox and rat were quantitatively analyzed by multiplex real-time PCR method, showing good accuracy (R.E. from 1.64% to 18.43%) and precision (R.S.D. from 0.51% to 12.70%). The method developed in this study will provide a powerful technology to combat meat adulteration and protect consumer health.
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a multiplex real time pcr approach for identification and quantification of sheep goat fox and murine fractions in meats using Nuclear DNA sequences
Food Control, 2021Co-Authors: Wenjun Wang, Xinyi Wang, Tiean Wei, Qingde Zhang, Xiang Zhou, Bang LiuAbstract:Abstract Adulterating undeclared animal components into meats may cause health problems. In this study, Nuclear DNA sequences for specific detection of sheep/goat, fox and murine (mouse, rat and hamster) were selected to design primers and probes for determination of mutton adulteration. The inter-specific specificity and intra-specific conservation were strictly evaluated in 19 different animal species and breeds, respectively. These three Nuclear DNA sequences were confirmed to be fixed copy sequences by relative quantitative PCR. Furthermore, a multiplex qualitative and quantitative PCR system with sensitivity of 0.05 ng was developed to efficiently discriminate sheep/goat (237 bp), fox (211 bp) and murine (160 bp) in one reaction. Finally, several mixed DNA samples of goat, fox and rat were quantitatively analyzed by multiplex real-time PCR method, showing good accuracy (R.E. from 1.64% to 18.43%) and precision (R.S.D. from 0.51% to 12.70%). The method developed in this study will provide a powerful technology to combat meat adulteration and protect consumer health.
