The Experts below are selected from a list of 9480 Experts worldwide ranked by ideXlab platform
Eduard Gershburg - One of the best experts on this subject based on the ideXlab platform.
-
the wild type autographa californica Nuclear Polyhedrosis Virus induces apoptosis of spodoptera littoralis cells
Virology, 1995Co-Authors: Nor Chejanovsky, Eduard GershburgAbstract:Abstract Spodoptera littoralis cells infected with the Autographa californica multiple Nuclear Polyhedrosis Virus (AcMNPV) yielded significantly lower budded Virus titers than Spodoptera frugiperda -infected cells and produced very low levels of polyhedrin. Relative to AcMNPV-infected S. frugiperda SF9 cells vital DNA replication was severely reduced in Spodoptera littoralis SL2 cells. Microscopic examination of SL2-infected cells revealed progressive cell blebbing starting at 6-8 hr postinfection and culminating in total cell destruction at 24 hr postinfection. The data suggested that AcMNPV-infected SL2 cells undergo apoptosis. The occurrence of an active apoptotic process in the infected cells was confirmed by: (1) observation of fragmentation of the cell nuclei stained with the specific fluorescent dye DAPI (4′,6′-diamidino-2-phenylindole) and (2) the presence of low-molecular-weight DNA oligomers. Neither SL2 cells infected with S. littoralis Nuclear Polyhedrosis Virus (SINPV) nor SF9 cells infected with AcMNPV, respectively, showed Nuclear fragmentation or oligonucleosomal ladder formation.
Susumu Maeda - One of the best experts on this subject based on the ideXlab platform.
-
characteristically distinct isolates of the Nuclear Polyhedrosis Virus from spodoptera litura
Journal of General Virology, 1990Co-Authors: Susumu Maeda, Yukuo Mukohara, Atsushi KondoAbstract:More than 100 isolates were plaque-purified to examine the genetic variations in four wild stocks of Spodoptera litura. Nuclear Polyhedrosis Virus (NPV) collected in Japan. These isolates were characterized by their in vitro host range in three established insect cell lines, growth characteristics, polyhedral protein, DNA restriction endonuclease pattern and DNA hybridization. The isolates were separated into four distinct groups: (I) isolates corresponding to Autographa californica NPV, (II and IV) two different groups of isolates of S. littoralis NPV which had been previously characterized and (III) isolates with no correspondence to any reported Virus group. Of the S. litura NPV wild stocks, two were mixtures of more than two different groups of NPVs. We have discussed the advantage of having a mixture of different NPV groups in the same wild Virus stocks.
-
molecular cloning and physical mapping of the genome of bombyx mori Nuclear Polyhedrosis Virus
Journal of General Virology, 1990Co-Authors: Susumu Maeda, Kei MajimaAbstract:A restriction fragment library which covered the entire genome of Bombyx mori Nuclear Polyhedrosis Virus (BmNPV) was constructed using plasmid vectors. By analysis of cloned and viral DNA by double digestion with endonucleases and by hybridization techniques, a complete physical map of BmNPV was constructed for BamHI, EcoRI, HindIII, KpnI, PstI and SmaI. Five regions of repeated sequences containing EcoRI sites were also found and mapped.
Atsushi Kondo - One of the best experts on this subject based on the ideXlab platform.
-
characteristically distinct isolates of the Nuclear Polyhedrosis Virus from spodoptera litura
Journal of General Virology, 1990Co-Authors: Susumu Maeda, Yukuo Mukohara, Atsushi KondoAbstract:More than 100 isolates were plaque-purified to examine the genetic variations in four wild stocks of Spodoptera litura. Nuclear Polyhedrosis Virus (NPV) collected in Japan. These isolates were characterized by their in vitro host range in three established insect cell lines, growth characteristics, polyhedral protein, DNA restriction endonuclease pattern and DNA hybridization. The isolates were separated into four distinct groups: (I) isolates corresponding to Autographa californica NPV, (II and IV) two different groups of isolates of S. littoralis NPV which had been previously characterized and (III) isolates with no correspondence to any reported Virus group. Of the S. litura NPV wild stocks, two were mixtures of more than two different groups of NPVs. We have discussed the advantage of having a mixture of different NPV groups in the same wild Virus stocks.
Robert D Possee - One of the best experts on this subject based on the ideXlab platform.
-
the complete dna sequence of autographa californica Nuclear Polyhedrosis Virus
Virology, 1994Co-Authors: Martin D Ayres, John Kuzio, Stephen C Howard, M Lopezferber, Robert D PosseeAbstract:The complete nucleotide sequence of the genome of clone 6 of the baculoVirus Autographa californica Nuclear Polyhedrosis Virus (AcNPV) has been determined. The molecule comprises 133,894 base pairs and has an overall A + T content of 59%. Our analysis suggests that the Virus encodes some 154 methionine-initiated, and potentially expressed, open reading frames (ORFs) of 150 nucleotides or greater. These ORFs are distributed evenly throughout the Virus genome on either strand. The ORFs are arranged as adjacent, nonoverlapping reading frames separated by short intergenic regions. Based on the primary nucleotide sequence, predictions have been made concerning the functions of certain genes, the sites for initiation of viral DNA replication, the regulation of early and late gene transcription, and factors that may affect the AcNPV gene translational efficiency. The genome sequence data confirm, with minor differences, the information obtained for other AcNPV clones. It is proposed that clone C6 is considered the archetype AcNPV for comparison purposes.
Michelle M Berner - One of the best experts on this subject based on the ideXlab platform.
-
identification of baculoVirus gene that promotes autographa californica Nuclear Polyhedrosis Virus replication in a nonpermissive insect cell line
Journal of Virology, 1996Co-Authors: Suzanne M Thiem, Martha E Quentin, Michelle M BernerAbstract:A gene that promotes Autographa californica M Nuclear Polyhedrosis Virus (AcMNPV) replication in IPLB-Ld652Y cells, a cell line that is nonpermissive for AcMNPV, was identified in Lymantria dispar M Nuclear Polyhedrosis Virus (LdMNPV). Cotransfection of AcMNPV DNA and a plasmid carrying the LdMNPV gene into IPLB-Ld652Y cells results in AcMNPV replication. The gene maps between 43.3 and 43.8 map units on the 162-kbp genome of LdMNPV. It comprises a 218-codon open reading frame and encodes a polypeptide with a predicted molecular mass of 25.7 kDa. The predicted polypeptide is glutamic acid and valine rich and negatively charged, with a pI of 4.61. No protein sequence motifs were identified, and no matches with known nucleotide or peptide sequences were found in the AcMNPV genome or database searches that suggest how this gene might function. A recombinant AcMNPV bearing the LdMNPV gene overcomes a block in protein synthesis observed in AcMNPV-infected IPLB-Ld652Y cells. Using Southern blotting techniques, we were unable to identify a homolog in Orgyia pseudotsugata M Nuclear Polyhedrosis Virus, a baculoVirus that is routinely propagated in IPLB-Ld652Y cells. This suggests that the LdMNPV host range is unique among the baculoViruses studied to date. We named this gene hrf-1 (for host range factor 1).
