The Experts below are selected from a list of 84 Experts worldwide ranked by ideXlab platform
Huang Min - One of the best experts on this subject based on the ideXlab platform.
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Effects of Nitrogen Forms on Grain Nucleic Acid Metabolism and Quality in Strong Gluten Wheat Variety‘Zhengmai 9023'
Journal of Nuclear Agricultural Sciences, 2013Co-Authors: Huang MinAbstract:A field experiment was conducted from 2009 to 2011 to investigate the regulatory effects of nitrogen forms on grain Nucleic Acid Metabolism and quality of strong gluten wheat variety Zhengmai 9023 and to screen optimal nitrogen form for high grain quality of this variety. The results showed that application with the nitrate nitrogen had the highest promoting effects on the total Nucleic Acid content,DNA and RNA content,and the basal Metabolism in grains. Nitrate nitrogen treatment significantly enhanced the soluble protein content in grains of Zhengmai 9023 during later grain filling period,and improved the synthesis of protein,the content of globulin and glutamine,and glutenin / gliadin ratio,thus leading to stronger flour extensograph and farinograph characteristics,and higher grain qualities. Application with ammonium nitrogen also could improve the extensograph and farinograph characteristics of Zhengmai 9023.
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effects of nitrogen forms on grain Nucleic Acid Metabolism and quality in strong gluten wheat variety zhengmai 9023
Journal of Nuclear Agricultural Sciences, 2013Co-Authors: Huang MinAbstract:A field experiment was conducted from 2009 to 2011 to investigate the regulatory effects of nitrogen forms on grain Nucleic Acid Metabolism and quality of strong gluten wheat variety Zhengmai 9023 and to screen optimal nitrogen form for high grain quality of this variety. The results showed that application with the nitrate nitrogen had the highest promoting effects on the total Nucleic Acid content,DNA and RNA content,and the basal Metabolism in grains. Nitrate nitrogen treatment significantly enhanced the soluble protein content in grains of Zhengmai 9023 during later grain filling period,and improved the synthesis of protein,the content of globulin and glutamine,and glutenin / gliadin ratio,thus leading to stronger flour extensograph and farinograph characteristics,and higher grain qualities. Application with ammonium nitrogen also could improve the extensograph and farinograph characteristics of Zhengmai 9023.
Robert M Brosh - One of the best experts on this subject based on the ideXlab platform.
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interactive roles of dna helicases and translocases with the single stranded dna binding protein rpa in Nucleic Acid Metabolism
International Journal of Molecular Sciences, 2017Co-Authors: Sanket Awate, Robert M BroshAbstract:Helicases and translocases use the energy of nucleoside triphosphate binding and hydrolysis to unwind/resolve structured Nucleic Acids or move along a single-stranded or double-stranded polynucleotide chain, respectively. These molecular motors facilitate a variety of transactions including replication, DNA repair, recombination, and transcription. A key partner of eukaryotic DNA helicases/translocases is the single-stranded DNA binding protein Replication Protein A (RPA). Biochemical, genetic, and cell biological assays have demonstrated that RPA interacts with these human molecular motors physically and functionally, and their association is enriched in cells undergoing replication stress. The roles of DNA helicases/translocases are orchestrated with RPA in pathways of Nucleic Acid Metabolism. RPA stimulates helicase-catalyzed DNA unwinding, enlists translocases to sites of action, and modulates their activities in DNA repair, fork remodeling, checkpoint activation, and telomere maintenance. The dynamic interplay between DNA helicases/translocases and RPA is just beginning to be understood at the molecular and cellular levels, and there is still much to be learned, which may inform potential therapeutic strategies.
Rajani Kant Chittela - One of the best experts on this subject based on the ideXlab platform.
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Translin: A multifunctional protein involved in Nucleic Acid Metabolism.
Journal of biosciences, 2019Co-Authors: Alka Gupta, Vinayaki S. Pillai, Rajani Kant ChittelaAbstract:Translin, a highly conserved, DNA/RNA binding protein, is abundantly expressed in brain, testis and in certain malignancies. It was discovered initially in the quest to find proteins that bind to alternating polypurines-polypyrimidines repeats. It has been implicated to have a role in RNA Metabolism (tRNA processing, RNAi, RNA transport, etc.), transcription, DNA damage response, etc. Studies from human, mice, drosophila and yeast have revealed that it forms an octameric ring, which is important for its function. Translin is a cytoplasmic protein, but under genotoxic stress, it migrates into the nucleus, binds to the break point hot spots and therefore, thought to be involved in chromosomal translocation events as well as DNA damage related response. Its structure is known and DNA binding regions, GTP binding region and regions responsible for homotypic and heterotypic interaction are known. It forms a ball like structure with open central channel for accommodating the substrate Nucleic Acids. Besides this, translin protein binds to 3' and 5' UTR of certain mRNAs and probably regulates their availability for translation. It is also involved in mRNA transport and cell cycle progression. It forms a heteromeric complex with translin associated factor-X (TRAX) to form C3PO complex which is involved in RNA silencing process. Recently, it has been shown that translin is upregulated under starvation conditions in Drosophila and is involved in the integration of sleep and metabolic rate of the flies. Earlier studies classified translin as a DNA repair protein; however subsequent studies showed that it is a multifunctional protein. With this background, in this review we have summarized the translin biochemical activities, cellular function as well as structural properties of this important protein.
Ping Cheng - One of the best experts on this subject based on the ideXlab platform.
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hepatitis b virus x protein hbx induced abnormalities of Nucleic Acid Metabolism revealed by 1 h nmr based metabonomics
Scientific Reports, 2016Co-Authors: Dan Yue, Yuwei Zhang, Liuliu Cheng, Liping Yang, Bin Shao, Anliang Huang, Rong Xiang, Ping ChengAbstract:Hepatitis B virus X protein (HBx) plays an important role in HBV-related hepatocarcinogenesis; however, mechanisms underlying HBx-mediated carcinogenesis remain unclear. In this study, an NMR-based metabolomics approach was applied to systematically investigate the effects of HBx on cell Metabolism. EdU incorporation assay was conducted to examine the effects of HBx on DNA synthesis, an important feature of Nucleic Acid Metabolism. The results revealed that HBx disrupted Metabolism of glucose, lipids, and amino Acids, especially Nucleic Acids. To understand the potential mechanism of HBx-induced abnormalities of Nucleic Acid Metabolism, gene expression profiles of HepG2 cells expressing HBx were investigated. The results showed that 29 genes involved in DNA damage and DNA repair were differentially expressed in HBx-expressing HepG2 cells. HBx-induced DNA damage was further demonstrated by karyotyping, comet assay, Western blotting, immunofluorescence and immunohistochemistry analyses. Many studies have previously reported that DNA damage can induce abnormalities of Nucleic Acid Metabolism. Thus, our results implied that HBx initially induces DNA damage, and then disrupts Nucleic Acid Metabolism, which in turn blocks DNA repair and induces the occurrence of hepatocellular carcinoma (HCC). These findings further contribute to our understanding of the occurrence of HCC.
James A. Borowiec - One of the best experts on this subject based on the ideXlab platform.
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Replication Protein A (RPA): The Eukaryotic SSB
Critical reviews in biochemistry and molecular biology, 1999Co-Authors: Cristina Iftode, Yaron Daniely, James A. BorowiecAbstract:Replication protein A (RPA) is a heterotrimeric single-stranded DNA-binding protein that is highly conserved in eukaryotes. RPA plays essential roles in many aspects of Nucleic Acid Metabolism, including DNA replication, nucleotide excision repair, and homologous recombination. In this review, we provide a comprehensive overview of RPA structure and function and highlight the more recent developments in these areas. The last few years have seen major advances in our understanding of the mechanism of RPA binding to DNA, including the structural characterization of the primary DNA-binding domains (DBD) and the identification of two secondary DBDs. Moreover, evidence indicates that RPA utilizes a multistep pathway to bind single-stranded DNA involving a particular molecular polarity of RPA, a mechanism that is apparently used to facilitate origin denaturation. In addition to its mechanistic roles, RPA interacts with many key factors in Nucleic Acid Metabolism, and we discuss the critical nature of many of th...
