The Experts below are selected from a list of 15 Experts worldwide ranked by ideXlab platform
Masayuki Masutani - One of the best experts on this subject based on the ideXlab platform.
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basic study on the chemosensitivity test by miniaturized improved Nucleic Acid Precursor incorporation assay mini assay
Gan to kagaku ryoho. Cancer & chemotherapy, 1990Co-Authors: M Mogi, M Motohashi, T Hirota, K Ohshima, Noriaki Takahashi, Yasushi Kinoshita, M Tanigawa, Takashi Yoshinobu, Y Koya, Masayuki MasutaniAbstract:UNLABELLED: Miniaturized improved Nucleic Acid Precursor incorporation assay (MINI assay) has been developed by Kern D. H. and Tanigawa N. et al since 1985. We investigated in vitro effects of mitomycin C (MMC), cisplatin (CDDP) and bleomycin (BLM) against A 549 lung cancer cells and HeLa cells by MINI assay. RESULTS: 3H-thymidine (3H-TdR) uptake in the positive control was 734.2 cpm +/- 10 cpm. The cut-off level for in vitro sensitivity was defined as more than 80% inhibition of 3H-TdR uptake in the drug treated cells compared to the positive controls. A 549 cells were sensitive to MMC (81.6%), CDDP (78.6%), but not to BLM (-57%). HeLa cells were sensitive to MMC (91%), CDDP (79.4%), but not to BLM (43%). MINI assay could be done with fewer cells than other methods and the results were obtained within 5 days. This method was considered to be useful for the chemosensitivity test with human tumors.
M Mogi - One of the best experts on this subject based on the ideXlab platform.
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basic study on the chemosensitivity test by miniaturized improved Nucleic Acid Precursor incorporation assay mini assay
Gan to kagaku ryoho. Cancer & chemotherapy, 1990Co-Authors: M Mogi, M Motohashi, T Hirota, K Ohshima, Noriaki Takahashi, Yasushi Kinoshita, M Tanigawa, Takashi Yoshinobu, Y Koya, Masayuki MasutaniAbstract:UNLABELLED: Miniaturized improved Nucleic Acid Precursor incorporation assay (MINI assay) has been developed by Kern D. H. and Tanigawa N. et al since 1985. We investigated in vitro effects of mitomycin C (MMC), cisplatin (CDDP) and bleomycin (BLM) against A 549 lung cancer cells and HeLa cells by MINI assay. RESULTS: 3H-thymidine (3H-TdR) uptake in the positive control was 734.2 cpm +/- 10 cpm. The cut-off level for in vitro sensitivity was defined as more than 80% inhibition of 3H-TdR uptake in the drug treated cells compared to the positive controls. A 549 cells were sensitive to MMC (81.6%), CDDP (78.6%), but not to BLM (-57%). HeLa cells were sensitive to MMC (91%), CDDP (79.4%), but not to BLM (43%). MINI assay could be done with fewer cells than other methods and the results were obtained within 5 days. This method was considered to be useful for the chemosensitivity test with human tumors.
M Motohashi - One of the best experts on this subject based on the ideXlab platform.
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basic study on the chemosensitivity test by miniaturized improved Nucleic Acid Precursor incorporation assay mini assay
Gan to kagaku ryoho. Cancer & chemotherapy, 1990Co-Authors: M Mogi, M Motohashi, T Hirota, K Ohshima, Noriaki Takahashi, Yasushi Kinoshita, M Tanigawa, Takashi Yoshinobu, Y Koya, Masayuki MasutaniAbstract:UNLABELLED: Miniaturized improved Nucleic Acid Precursor incorporation assay (MINI assay) has been developed by Kern D. H. and Tanigawa N. et al since 1985. We investigated in vitro effects of mitomycin C (MMC), cisplatin (CDDP) and bleomycin (BLM) against A 549 lung cancer cells and HeLa cells by MINI assay. RESULTS: 3H-thymidine (3H-TdR) uptake in the positive control was 734.2 cpm +/- 10 cpm. The cut-off level for in vitro sensitivity was defined as more than 80% inhibition of 3H-TdR uptake in the drug treated cells compared to the positive controls. A 549 cells were sensitive to MMC (81.6%), CDDP (78.6%), but not to BLM (-57%). HeLa cells were sensitive to MMC (91%), CDDP (79.4%), but not to BLM (43%). MINI assay could be done with fewer cells than other methods and the results were obtained within 5 days. This method was considered to be useful for the chemosensitivity test with human tumors.
T Hirota - One of the best experts on this subject based on the ideXlab platform.
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basic study on the chemosensitivity test by miniaturized improved Nucleic Acid Precursor incorporation assay mini assay
Gan to kagaku ryoho. Cancer & chemotherapy, 1990Co-Authors: M Mogi, M Motohashi, T Hirota, K Ohshima, Noriaki Takahashi, Yasushi Kinoshita, M Tanigawa, Takashi Yoshinobu, Y Koya, Masayuki MasutaniAbstract:UNLABELLED: Miniaturized improved Nucleic Acid Precursor incorporation assay (MINI assay) has been developed by Kern D. H. and Tanigawa N. et al since 1985. We investigated in vitro effects of mitomycin C (MMC), cisplatin (CDDP) and bleomycin (BLM) against A 549 lung cancer cells and HeLa cells by MINI assay. RESULTS: 3H-thymidine (3H-TdR) uptake in the positive control was 734.2 cpm +/- 10 cpm. The cut-off level for in vitro sensitivity was defined as more than 80% inhibition of 3H-TdR uptake in the drug treated cells compared to the positive controls. A 549 cells were sensitive to MMC (81.6%), CDDP (78.6%), but not to BLM (-57%). HeLa cells were sensitive to MMC (91%), CDDP (79.4%), but not to BLM (43%). MINI assay could be done with fewer cells than other methods and the results were obtained within 5 days. This method was considered to be useful for the chemosensitivity test with human tumors.
K Ohshima - One of the best experts on this subject based on the ideXlab platform.
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basic study on the chemosensitivity test by miniaturized improved Nucleic Acid Precursor incorporation assay mini assay
Gan to kagaku ryoho. Cancer & chemotherapy, 1990Co-Authors: M Mogi, M Motohashi, T Hirota, K Ohshima, Noriaki Takahashi, Yasushi Kinoshita, M Tanigawa, Takashi Yoshinobu, Y Koya, Masayuki MasutaniAbstract:UNLABELLED: Miniaturized improved Nucleic Acid Precursor incorporation assay (MINI assay) has been developed by Kern D. H. and Tanigawa N. et al since 1985. We investigated in vitro effects of mitomycin C (MMC), cisplatin (CDDP) and bleomycin (BLM) against A 549 lung cancer cells and HeLa cells by MINI assay. RESULTS: 3H-thymidine (3H-TdR) uptake in the positive control was 734.2 cpm +/- 10 cpm. The cut-off level for in vitro sensitivity was defined as more than 80% inhibition of 3H-TdR uptake in the drug treated cells compared to the positive controls. A 549 cells were sensitive to MMC (81.6%), CDDP (78.6%), but not to BLM (-57%). HeLa cells were sensitive to MMC (91%), CDDP (79.4%), but not to BLM (43%). MINI assay could be done with fewer cells than other methods and the results were obtained within 5 days. This method was considered to be useful for the chemosensitivity test with human tumors.
