The Experts below are selected from a list of 8334 Experts worldwide ranked by ideXlab platform
Eric Peyrin - One of the best experts on this subject based on the ideXlab platform.
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rationally designed aptamer based fluorescence polarization sensor dedicated to the small target analysis
Biosensors and Bioelectronics, 2010Co-Authors: Sandrine Perrier, Corinne Ravelet, Jennifer Fize, Valerie Guieu, Christian Perigaud, Eric PeyrinAbstract:Abstract A direct fluorescence polarization (FP) assay strategy, dedicated to the small molecule sensing and based on the unique induced-fit binding mechanism of end-labelled Nucleic Acid aptamers, has been recently developed by our group. Small target binding has been successfully converted into a significant increase of the fluorescence anisotropy signal presumably produced by the reduction of the local motional freedom of the dye. In order to generalize the approach, a rational FP sensor methodology was established herein, by engineering instability in the secondary structure of an aptameric recognition element. The anti-adenosine DNA aptamer, labelled by a single fluorescein dye at its 3′ extremity, was employed as a model functional Nucleic Acid Probe. The terminal stem of the stem-loop structure was shortened to induce a destabilized/denatured conformation which promoted the local segmental mobility of the dye and then a significant depolarization process. Upon target binding, the structural change of the aptamer induced the formation of a stable stem-loop structure, leading to the reduction of the dye mobility and the increase in the fluorescence anisotropy signal. This reasoned approach was applied to the sensing of adenosine and adenosine monophosphate and their chiral analysis.
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Noncompetitive Fluorescence Polarization Aptamer-based Assay for Small Molecule Detection
Analytical Chemistry, 2009Co-Authors: Josephine Ruta, Sandrine Perrier, Corinne Ravelet, Jennifer Fize, Eric PeyrinAbstract:In this paper, a new fluorescence polarization (FP) assay strategy is described reporting the first demonstration of a noncompetitive FP technique dedicated to the small molecule sensing. This approach was based on the unique induced-fit binding mechanism of Nucleic Acid aptamers which was exploited to convert the small target binding event into a detectable fluorescence anisotropy signal. An anti-l-tyrosinamide DNA aptamer, labeled by a single fluorescent dye at its extremity, was employed as a model functional Nucleic Acid Probe. The DNA conformational change generated by the l-tyrosinamide binding was able to induce a significant increase in the fluorescence anisotropy signal. The method allowed enantioselective sensing of tyrosinamide and analysis in practical samples. The methodology was also applied to the l-argininamide detection, suggesting the potential generalizability of the direct FP-based strategy. Such aptamer-based assay appeared to be a sensitive analytical system of remarkable simplicity and ease of use.
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noncompetitive fluorescence polarization aptamer based assay for small molecule detection
Analytical Chemistry, 2009Co-Authors: Josephine Ruta, Sandrine Perrier, Corinne Ravelet, Jennifer Fize, Eric PeyrinAbstract:In this paper, a new fluorescence polarization (FP) assay strategy is described reporting the first demonstration of a noncompetitive FP technique dedicated to the small molecule sensing. This approach was based on the unique induced-fit binding mechanism of Nucleic Acid aptamers which was exploited to convert the small target binding event into a detectable fluorescence anisotropy signal. An anti-l-tyrosinamide DNA aptamer, labeled by a single fluorescent dye at its extremity, was employed as a model functional Nucleic Acid Probe. The DNA conformational change generated by the l-tyrosinamide binding was able to induce a significant increase in the fluorescence anisotropy signal. The method allowed enantioselective sensing of tyrosinamide and analysis in practical samples. The methodology was also applied to the l-argininamide detection, suggesting the potential generalizability of the direct FP-based strategy. Such aptamer-based assay appeared to be a sensitive analytical system of remarkable simplicity ...
Sandrine Perrier - One of the best experts on this subject based on the ideXlab platform.
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rationally designed aptamer based fluorescence polarization sensor dedicated to the small target analysis
Biosensors and Bioelectronics, 2010Co-Authors: Sandrine Perrier, Corinne Ravelet, Jennifer Fize, Valerie Guieu, Christian Perigaud, Eric PeyrinAbstract:Abstract A direct fluorescence polarization (FP) assay strategy, dedicated to the small molecule sensing and based on the unique induced-fit binding mechanism of end-labelled Nucleic Acid aptamers, has been recently developed by our group. Small target binding has been successfully converted into a significant increase of the fluorescence anisotropy signal presumably produced by the reduction of the local motional freedom of the dye. In order to generalize the approach, a rational FP sensor methodology was established herein, by engineering instability in the secondary structure of an aptameric recognition element. The anti-adenosine DNA aptamer, labelled by a single fluorescein dye at its 3′ extremity, was employed as a model functional Nucleic Acid Probe. The terminal stem of the stem-loop structure was shortened to induce a destabilized/denatured conformation which promoted the local segmental mobility of the dye and then a significant depolarization process. Upon target binding, the structural change of the aptamer induced the formation of a stable stem-loop structure, leading to the reduction of the dye mobility and the increase in the fluorescence anisotropy signal. This reasoned approach was applied to the sensing of adenosine and adenosine monophosphate and their chiral analysis.
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Noncompetitive Fluorescence Polarization Aptamer-based Assay for Small Molecule Detection
Analytical Chemistry, 2009Co-Authors: Josephine Ruta, Sandrine Perrier, Corinne Ravelet, Jennifer Fize, Eric PeyrinAbstract:In this paper, a new fluorescence polarization (FP) assay strategy is described reporting the first demonstration of a noncompetitive FP technique dedicated to the small molecule sensing. This approach was based on the unique induced-fit binding mechanism of Nucleic Acid aptamers which was exploited to convert the small target binding event into a detectable fluorescence anisotropy signal. An anti-l-tyrosinamide DNA aptamer, labeled by a single fluorescent dye at its extremity, was employed as a model functional Nucleic Acid Probe. The DNA conformational change generated by the l-tyrosinamide binding was able to induce a significant increase in the fluorescence anisotropy signal. The method allowed enantioselective sensing of tyrosinamide and analysis in practical samples. The methodology was also applied to the l-argininamide detection, suggesting the potential generalizability of the direct FP-based strategy. Such aptamer-based assay appeared to be a sensitive analytical system of remarkable simplicity and ease of use.
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noncompetitive fluorescence polarization aptamer based assay for small molecule detection
Analytical Chemistry, 2009Co-Authors: Josephine Ruta, Sandrine Perrier, Corinne Ravelet, Jennifer Fize, Eric PeyrinAbstract:In this paper, a new fluorescence polarization (FP) assay strategy is described reporting the first demonstration of a noncompetitive FP technique dedicated to the small molecule sensing. This approach was based on the unique induced-fit binding mechanism of Nucleic Acid aptamers which was exploited to convert the small target binding event into a detectable fluorescence anisotropy signal. An anti-l-tyrosinamide DNA aptamer, labeled by a single fluorescent dye at its extremity, was employed as a model functional Nucleic Acid Probe. The DNA conformational change generated by the l-tyrosinamide binding was able to induce a significant increase in the fluorescence anisotropy signal. The method allowed enantioselective sensing of tyrosinamide and analysis in practical samples. The methodology was also applied to the l-argininamide detection, suggesting the potential generalizability of the direct FP-based strategy. Such aptamer-based assay appeared to be a sensitive analytical system of remarkable simplicity ...
Jennifer Fize - One of the best experts on this subject based on the ideXlab platform.
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rationally designed aptamer based fluorescence polarization sensor dedicated to the small target analysis
Biosensors and Bioelectronics, 2010Co-Authors: Sandrine Perrier, Corinne Ravelet, Jennifer Fize, Valerie Guieu, Christian Perigaud, Eric PeyrinAbstract:Abstract A direct fluorescence polarization (FP) assay strategy, dedicated to the small molecule sensing and based on the unique induced-fit binding mechanism of end-labelled Nucleic Acid aptamers, has been recently developed by our group. Small target binding has been successfully converted into a significant increase of the fluorescence anisotropy signal presumably produced by the reduction of the local motional freedom of the dye. In order to generalize the approach, a rational FP sensor methodology was established herein, by engineering instability in the secondary structure of an aptameric recognition element. The anti-adenosine DNA aptamer, labelled by a single fluorescein dye at its 3′ extremity, was employed as a model functional Nucleic Acid Probe. The terminal stem of the stem-loop structure was shortened to induce a destabilized/denatured conformation which promoted the local segmental mobility of the dye and then a significant depolarization process. Upon target binding, the structural change of the aptamer induced the formation of a stable stem-loop structure, leading to the reduction of the dye mobility and the increase in the fluorescence anisotropy signal. This reasoned approach was applied to the sensing of adenosine and adenosine monophosphate and their chiral analysis.
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Noncompetitive Fluorescence Polarization Aptamer-based Assay for Small Molecule Detection
Analytical Chemistry, 2009Co-Authors: Josephine Ruta, Sandrine Perrier, Corinne Ravelet, Jennifer Fize, Eric PeyrinAbstract:In this paper, a new fluorescence polarization (FP) assay strategy is described reporting the first demonstration of a noncompetitive FP technique dedicated to the small molecule sensing. This approach was based on the unique induced-fit binding mechanism of Nucleic Acid aptamers which was exploited to convert the small target binding event into a detectable fluorescence anisotropy signal. An anti-l-tyrosinamide DNA aptamer, labeled by a single fluorescent dye at its extremity, was employed as a model functional Nucleic Acid Probe. The DNA conformational change generated by the l-tyrosinamide binding was able to induce a significant increase in the fluorescence anisotropy signal. The method allowed enantioselective sensing of tyrosinamide and analysis in practical samples. The methodology was also applied to the l-argininamide detection, suggesting the potential generalizability of the direct FP-based strategy. Such aptamer-based assay appeared to be a sensitive analytical system of remarkable simplicity and ease of use.
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noncompetitive fluorescence polarization aptamer based assay for small molecule detection
Analytical Chemistry, 2009Co-Authors: Josephine Ruta, Sandrine Perrier, Corinne Ravelet, Jennifer Fize, Eric PeyrinAbstract:In this paper, a new fluorescence polarization (FP) assay strategy is described reporting the first demonstration of a noncompetitive FP technique dedicated to the small molecule sensing. This approach was based on the unique induced-fit binding mechanism of Nucleic Acid aptamers which was exploited to convert the small target binding event into a detectable fluorescence anisotropy signal. An anti-l-tyrosinamide DNA aptamer, labeled by a single fluorescent dye at its extremity, was employed as a model functional Nucleic Acid Probe. The DNA conformational change generated by the l-tyrosinamide binding was able to induce a significant increase in the fluorescence anisotropy signal. The method allowed enantioselective sensing of tyrosinamide and analysis in practical samples. The methodology was also applied to the l-argininamide detection, suggesting the potential generalizability of the direct FP-based strategy. Such aptamer-based assay appeared to be a sensitive analytical system of remarkable simplicity ...
Corinne Ravelet - One of the best experts on this subject based on the ideXlab platform.
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rationally designed aptamer based fluorescence polarization sensor dedicated to the small target analysis
Biosensors and Bioelectronics, 2010Co-Authors: Sandrine Perrier, Corinne Ravelet, Jennifer Fize, Valerie Guieu, Christian Perigaud, Eric PeyrinAbstract:Abstract A direct fluorescence polarization (FP) assay strategy, dedicated to the small molecule sensing and based on the unique induced-fit binding mechanism of end-labelled Nucleic Acid aptamers, has been recently developed by our group. Small target binding has been successfully converted into a significant increase of the fluorescence anisotropy signal presumably produced by the reduction of the local motional freedom of the dye. In order to generalize the approach, a rational FP sensor methodology was established herein, by engineering instability in the secondary structure of an aptameric recognition element. The anti-adenosine DNA aptamer, labelled by a single fluorescein dye at its 3′ extremity, was employed as a model functional Nucleic Acid Probe. The terminal stem of the stem-loop structure was shortened to induce a destabilized/denatured conformation which promoted the local segmental mobility of the dye and then a significant depolarization process. Upon target binding, the structural change of the aptamer induced the formation of a stable stem-loop structure, leading to the reduction of the dye mobility and the increase in the fluorescence anisotropy signal. This reasoned approach was applied to the sensing of adenosine and adenosine monophosphate and their chiral analysis.
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Noncompetitive Fluorescence Polarization Aptamer-based Assay for Small Molecule Detection
Analytical Chemistry, 2009Co-Authors: Josephine Ruta, Sandrine Perrier, Corinne Ravelet, Jennifer Fize, Eric PeyrinAbstract:In this paper, a new fluorescence polarization (FP) assay strategy is described reporting the first demonstration of a noncompetitive FP technique dedicated to the small molecule sensing. This approach was based on the unique induced-fit binding mechanism of Nucleic Acid aptamers which was exploited to convert the small target binding event into a detectable fluorescence anisotropy signal. An anti-l-tyrosinamide DNA aptamer, labeled by a single fluorescent dye at its extremity, was employed as a model functional Nucleic Acid Probe. The DNA conformational change generated by the l-tyrosinamide binding was able to induce a significant increase in the fluorescence anisotropy signal. The method allowed enantioselective sensing of tyrosinamide and analysis in practical samples. The methodology was also applied to the l-argininamide detection, suggesting the potential generalizability of the direct FP-based strategy. Such aptamer-based assay appeared to be a sensitive analytical system of remarkable simplicity and ease of use.
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noncompetitive fluorescence polarization aptamer based assay for small molecule detection
Analytical Chemistry, 2009Co-Authors: Josephine Ruta, Sandrine Perrier, Corinne Ravelet, Jennifer Fize, Eric PeyrinAbstract:In this paper, a new fluorescence polarization (FP) assay strategy is described reporting the first demonstration of a noncompetitive FP technique dedicated to the small molecule sensing. This approach was based on the unique induced-fit binding mechanism of Nucleic Acid aptamers which was exploited to convert the small target binding event into a detectable fluorescence anisotropy signal. An anti-l-tyrosinamide DNA aptamer, labeled by a single fluorescent dye at its extremity, was employed as a model functional Nucleic Acid Probe. The DNA conformational change generated by the l-tyrosinamide binding was able to induce a significant increase in the fluorescence anisotropy signal. The method allowed enantioselective sensing of tyrosinamide and analysis in practical samples. The methodology was also applied to the l-argininamide detection, suggesting the potential generalizability of the direct FP-based strategy. Such aptamer-based assay appeared to be a sensitive analytical system of remarkable simplicity ...
Josephine Ruta - One of the best experts on this subject based on the ideXlab platform.
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Noncompetitive Fluorescence Polarization Aptamer-based Assay for Small Molecule Detection
Analytical Chemistry, 2009Co-Authors: Josephine Ruta, Sandrine Perrier, Corinne Ravelet, Jennifer Fize, Eric PeyrinAbstract:In this paper, a new fluorescence polarization (FP) assay strategy is described reporting the first demonstration of a noncompetitive FP technique dedicated to the small molecule sensing. This approach was based on the unique induced-fit binding mechanism of Nucleic Acid aptamers which was exploited to convert the small target binding event into a detectable fluorescence anisotropy signal. An anti-l-tyrosinamide DNA aptamer, labeled by a single fluorescent dye at its extremity, was employed as a model functional Nucleic Acid Probe. The DNA conformational change generated by the l-tyrosinamide binding was able to induce a significant increase in the fluorescence anisotropy signal. The method allowed enantioselective sensing of tyrosinamide and analysis in practical samples. The methodology was also applied to the l-argininamide detection, suggesting the potential generalizability of the direct FP-based strategy. Such aptamer-based assay appeared to be a sensitive analytical system of remarkable simplicity and ease of use.
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noncompetitive fluorescence polarization aptamer based assay for small molecule detection
Analytical Chemistry, 2009Co-Authors: Josephine Ruta, Sandrine Perrier, Corinne Ravelet, Jennifer Fize, Eric PeyrinAbstract:In this paper, a new fluorescence polarization (FP) assay strategy is described reporting the first demonstration of a noncompetitive FP technique dedicated to the small molecule sensing. This approach was based on the unique induced-fit binding mechanism of Nucleic Acid aptamers which was exploited to convert the small target binding event into a detectable fluorescence anisotropy signal. An anti-l-tyrosinamide DNA aptamer, labeled by a single fluorescent dye at its extremity, was employed as a model functional Nucleic Acid Probe. The DNA conformational change generated by the l-tyrosinamide binding was able to induce a significant increase in the fluorescence anisotropy signal. The method allowed enantioselective sensing of tyrosinamide and analysis in practical samples. The methodology was also applied to the l-argininamide detection, suggesting the potential generalizability of the direct FP-based strategy. Such aptamer-based assay appeared to be a sensitive analytical system of remarkable simplicity ...
