The Experts below are selected from a list of 51 Experts worldwide ranked by ideXlab platform
RT Smolenski - One of the best experts on this subject based on the ideXlab platform.
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metabolism of 4 pyridone 3 carboxamide 1 beta d ribonucleoside triphosphate and its nucleoside precursor in the erythrocytes
Nucleosides Nucleotides & Nucleic Acids, 2008Co-Authors: Ewa M Slominska, Czeslawa Orlewska, A H Y Yuen, Lana Osman, Pawel Romaszko, E Sokolowska, Henryk Foks, H A Simmonds, M H Yacoub, RT SmolenskiAbstract:We recently discovered new Nucleotides (4-pyridone-3-carboxamide-1-β -D-ribonucleoside phosphates) in human erythrocytes. To establish the precursor compound and pathways of Nucleotide Derivative formation and breakdown, human erythrocytes were incubated for 3 hours with 0.3 mM 4-pyridone-3-carboxamide-1-β -D-ribonucleoside (4PYR) and erythrocyte concentrations of 4PYR and adenine Nucleotides were followed. 4PYR triphosphate increased from 16.1 ± 0.6 μ M to 74.9 ± 9.17 and 4PYR monophosphate increased from 5 μ M to 254.7 ± 13.9 μ M. Conversely, incubation with 0.3 mM 4-pyridone-3-carboxamide (4PY) did not lead to additional 4PYR Nucleotide formation. 4PYR Nucleotides were catabolized to 4PYR. We conclude that 4PYR Nucleotides are formed in erythrocytes by nucleoside kinase-mediated 4PYR phosphorylation and catabolized by 5′nucleotidase-mediated dephosphorylation.
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Metabolism of 4-pyridone-3-carboxamide-1-beta-D-ribonucleoside triphosphate and its nucleoside precursor in the erythrocytes.
Nucleosides Nucleotides Nucleic Acids, 2008Co-Authors: RT SmolenskiAbstract:We recently discovered new Nucleotides (4-pyridone-3-carboxamide-1-beta -D-ribonucleoside phosphates) in human erythrocytes. To establish the precursor compound and pathways of Nucleotide Derivative formation and breakdown, human erythrocytes were incubated for 3 hours with 0.3 mM 4-pyridone-3-carboxamide-1-beta-D-ribonucleoside (4PYR) and erythrocyte concentrations of 4PYR and adenine Nucleotides were followed. 4PYR triphosphate increased from 16.1 +/- 0.6 micro M to 74.9 +/- 9.17 and 4PYR monophosphate increased from 5 micro M to 254.7 +/- 13.9 micro M. Conversely, incubation with 0.3 mM 4-pyridone-3-carboxamide (4PY) did not lead to additional 4PYR Nucleotide formation. 4PYR Nucleotides were catabolized to 4PYR. We conclude that 4PYR Nucleotides are formed in erythrocytes by nucleoside kinase-mediated 4PYR phosphorylation and catabolized by 5'nucleotidase-mediated dephosphorylation.
Ewa M Slominska - One of the best experts on this subject based on the ideXlab platform.
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metabolism of 4 pyridone 3 carboxamide 1 beta d ribonucleoside triphosphate and its nucleoside precursor in the erythrocytes
Nucleosides Nucleotides & Nucleic Acids, 2008Co-Authors: Ewa M Slominska, Czeslawa Orlewska, A H Y Yuen, Lana Osman, Pawel Romaszko, E Sokolowska, Henryk Foks, H A Simmonds, M H Yacoub, RT SmolenskiAbstract:We recently discovered new Nucleotides (4-pyridone-3-carboxamide-1-β -D-ribonucleoside phosphates) in human erythrocytes. To establish the precursor compound and pathways of Nucleotide Derivative formation and breakdown, human erythrocytes were incubated for 3 hours with 0.3 mM 4-pyridone-3-carboxamide-1-β -D-ribonucleoside (4PYR) and erythrocyte concentrations of 4PYR and adenine Nucleotides were followed. 4PYR triphosphate increased from 16.1 ± 0.6 μ M to 74.9 ± 9.17 and 4PYR monophosphate increased from 5 μ M to 254.7 ± 13.9 μ M. Conversely, incubation with 0.3 mM 4-pyridone-3-carboxamide (4PY) did not lead to additional 4PYR Nucleotide formation. 4PYR Nucleotides were catabolized to 4PYR. We conclude that 4PYR Nucleotides are formed in erythrocytes by nucleoside kinase-mediated 4PYR phosphorylation and catabolized by 5′nucleotidase-mediated dephosphorylation.
Georg Reiser - One of the best experts on this subject based on the ideXlab platform.
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5 ome uridine 5 o α boranodiphosphate a novel Nucleotide Derivative highly active at the human p2y6 receptor protects against death receptor mediated glial apoptosis
Neuroscience Letters, 2014Co-Authors: Michael Haas, Tamar Ginsburgshmuel, Bilha Fischer, Georg ReiserAbstract:Abstract P2Y receptors are activated by Nucleotides and involved in numerous physiological/pathophysiological processes. However, investigations of specific P2Y receptor functions have been hampered by lack of suitable receptor agonists–antagonists. Recently, we identified the Nucleotide 5-OMe-UDP as potent and selective agonist for human P2Y 6 receptors. We studied a series of Derivatives of this analog with a Pα-borano group substituting a non-bridging oxygen and found increased potency and receptor specificity. R p -5-OMe-UDPαB (R p -5-OMe-uridine 5′- O -α-boranodiphosphate) was most potent and selective in inducing intracellular calcium signaling in 1321N1 astrocytoma cells expressing the human P2Y 6 receptor. Here, we investigated whether R p -5-OMe-UDPαB evokes cell protection through human P2Y 6 receptors. We tested a well-established model, tumor necrosis factor α (TNFα)-induced cell death in 1321N1 astrocytoma cells. R p -5-OMe-UDPαB inhibited TNFα-induced cell death even stronger than UDP. These first data of a neuro-protective activity of the human P2Y 6 receptor emphasize the potential of the stable, selective, and potent R p -5-OMe-UDPαB analog for exploiting P2Y 6 receptor-mediated cellular functions, like cytoprotection in human tissues, with suitability for future neuro-protective drug development.
Pawel Romaszko - One of the best experts on this subject based on the ideXlab platform.
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metabolism of 4 pyridone 3 carboxamide 1 beta d ribonucleoside triphosphate and its nucleoside precursor in the erythrocytes
Nucleosides Nucleotides & Nucleic Acids, 2008Co-Authors: Ewa M Slominska, Czeslawa Orlewska, A H Y Yuen, Lana Osman, Pawel Romaszko, E Sokolowska, Henryk Foks, H A Simmonds, M H Yacoub, RT SmolenskiAbstract:We recently discovered new Nucleotides (4-pyridone-3-carboxamide-1-β -D-ribonucleoside phosphates) in human erythrocytes. To establish the precursor compound and pathways of Nucleotide Derivative formation and breakdown, human erythrocytes were incubated for 3 hours with 0.3 mM 4-pyridone-3-carboxamide-1-β -D-ribonucleoside (4PYR) and erythrocyte concentrations of 4PYR and adenine Nucleotides were followed. 4PYR triphosphate increased from 16.1 ± 0.6 μ M to 74.9 ± 9.17 and 4PYR monophosphate increased from 5 μ M to 254.7 ± 13.9 μ M. Conversely, incubation with 0.3 mM 4-pyridone-3-carboxamide (4PY) did not lead to additional 4PYR Nucleotide formation. 4PYR Nucleotides were catabolized to 4PYR. We conclude that 4PYR Nucleotides are formed in erythrocytes by nucleoside kinase-mediated 4PYR phosphorylation and catabolized by 5′nucleotidase-mediated dephosphorylation.
Czeslawa Orlewska - One of the best experts on this subject based on the ideXlab platform.
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metabolism of 4 pyridone 3 carboxamide 1 beta d ribonucleoside triphosphate and its nucleoside precursor in the erythrocytes
Nucleosides Nucleotides & Nucleic Acids, 2008Co-Authors: Ewa M Slominska, Czeslawa Orlewska, A H Y Yuen, Lana Osman, Pawel Romaszko, E Sokolowska, Henryk Foks, H A Simmonds, M H Yacoub, RT SmolenskiAbstract:We recently discovered new Nucleotides (4-pyridone-3-carboxamide-1-β -D-ribonucleoside phosphates) in human erythrocytes. To establish the precursor compound and pathways of Nucleotide Derivative formation and breakdown, human erythrocytes were incubated for 3 hours with 0.3 mM 4-pyridone-3-carboxamide-1-β -D-ribonucleoside (4PYR) and erythrocyte concentrations of 4PYR and adenine Nucleotides were followed. 4PYR triphosphate increased from 16.1 ± 0.6 μ M to 74.9 ± 9.17 and 4PYR monophosphate increased from 5 μ M to 254.7 ± 13.9 μ M. Conversely, incubation with 0.3 mM 4-pyridone-3-carboxamide (4PY) did not lead to additional 4PYR Nucleotide formation. 4PYR Nucleotides were catabolized to 4PYR. We conclude that 4PYR Nucleotides are formed in erythrocytes by nucleoside kinase-mediated 4PYR phosphorylation and catabolized by 5′nucleotidase-mediated dephosphorylation.
