The Experts below are selected from a list of 18870 Experts worldwide ranked by ideXlab platform
Jurgen Bendig - One of the best experts on this subject based on the ideXlab platform.
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7 dialkylamino coumarin 4 yl methyl caged compounds as ultrafast and effective long wavelength phototriggers of 8bromo substituted cyclic Nucleotides
ChemBioChem, 2003Co-Authors: Volker Hagen, Stephan Frings, Burkhard Wiesner, Siegrun Helm, Benjamin U Kaupp, Jurgen BendigAbstract:[7-(Dimethylamino)coumarin-4-yl]methyl (DMACM) and [7-(diethylamino)coumarin-4-yl]methyl (DEACM) esters of 8-bromoadenosine 3',5'-cyclic monophosphate (8-Br-cAMP) and 8-bromoguanosine 3',5'-cyclic monophosphate (8-Br-cGMP) are described as novel caged compounds for 8-bromo-substituted cyclic Nucleotides. Synthesis is accomplished by treatment of the free acids of the cyclic Nucleotides with the corresponding 7(dialkylamino)-substituted 4(diazomethyl)coumarins. Irradiation of the DMACM- and DEACM-caged cyclic Nucleotides with UV light stimulates the release of the cyclic Nucleotides within roughly a nanosecond. The new caged compounds are resistant to hydrolysis in aqueous buffers and exhibit long-wavelength absorption properties with maxima at 400 nm, high extinction coefficients, and high quantum yields (0.15-0.31). Their favorable properties render these compounds the most efficient and rapid phototriggers of 8-bromo-substituted cyclic Nucleotides known. The usefulness of the compounds for physiological studies under nondamaging light conditions was examined in HEK293 cells expressing the alpha subunit of the cyclic-nucleotide-gated (CNG) channel of cone photoreceptors (CNGA3) and of olfactory neurons (CNGA2) by using confocal laser scanning microscopy and the patch clamp technique.
Michal Hocek - One of the best experts on this subject based on the ideXlab platform.
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additions of thiols to 7 vinyl 7 deazaadenine nucleosides and Nucleotides Synthesis of hydrophobic derivatives of 2 deoxyadenosine datp and dna
Journal of Organic Chemistry, 2016Co-Authors: Michaela Slavickova, Radek Pohl, Michal HocekAbstract:Additions of alkyl- or arylthiols to 7-vinyl-7-deaza-2′-deoxyadenosine gave a series of 7-[2-(alkyl- or arylsulfanyl)ethyl]-7-deaza-2′-deoxyadenosines in 45–85% yields. The nucleosides were converted to 5′-O-mono-(dASRMP) or triphosphates (dASRTP) by phosphorylation. The modified triphosphates were also prepared by thiol addition to 7-vinyl-7-deaza-dATP. The triphosphates dASRTP were good substrates for DNA polymerases useful in the enzymatic Synthesis of base-modified oligoNucleotides (ONs) or DNA containing flexibly linked hydrophobic substituents in the major groove. Primer extension was used for the Synthesis of ONs with one or several modifications, PCR was used for the Synthesis of heavily modified DNA, whereas terminal deoxynucleotidyl transferase was used for a single-nucleotide labeling of the 3′-end.
Volker Hagen - One of the best experts on this subject based on the ideXlab platform.
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7 dialkylamino coumarin 4 yl methyl caged compounds as ultrafast and effective long wavelength phototriggers of 8bromo substituted cyclic Nucleotides
ChemBioChem, 2003Co-Authors: Volker Hagen, Stephan Frings, Burkhard Wiesner, Siegrun Helm, Benjamin U Kaupp, Jurgen BendigAbstract:[7-(Dimethylamino)coumarin-4-yl]methyl (DMACM) and [7-(diethylamino)coumarin-4-yl]methyl (DEACM) esters of 8-bromoadenosine 3',5'-cyclic monophosphate (8-Br-cAMP) and 8-bromoguanosine 3',5'-cyclic monophosphate (8-Br-cGMP) are described as novel caged compounds for 8-bromo-substituted cyclic Nucleotides. Synthesis is accomplished by treatment of the free acids of the cyclic Nucleotides with the corresponding 7(dialkylamino)-substituted 4(diazomethyl)coumarins. Irradiation of the DMACM- and DEACM-caged cyclic Nucleotides with UV light stimulates the release of the cyclic Nucleotides within roughly a nanosecond. The new caged compounds are resistant to hydrolysis in aqueous buffers and exhibit long-wavelength absorption properties with maxima at 400 nm, high extinction coefficients, and high quantum yields (0.15-0.31). Their favorable properties render these compounds the most efficient and rapid phototriggers of 8-bromo-substituted cyclic Nucleotides known. The usefulness of the compounds for physiological studies under nondamaging light conditions was examined in HEK293 cells expressing the alpha subunit of the cyclic-nucleotide-gated (CNG) channel of cone photoreceptors (CNGA3) and of olfactory neurons (CNGA2) by using confocal laser scanning microscopy and the patch clamp technique.
Michaela Slavickova - One of the best experts on this subject based on the ideXlab platform.
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additions of thiols to 7 vinyl 7 deazaadenine nucleosides and Nucleotides Synthesis of hydrophobic derivatives of 2 deoxyadenosine datp and dna
Journal of Organic Chemistry, 2016Co-Authors: Michaela Slavickova, Radek Pohl, Michal HocekAbstract:Additions of alkyl- or arylthiols to 7-vinyl-7-deaza-2′-deoxyadenosine gave a series of 7-[2-(alkyl- or arylsulfanyl)ethyl]-7-deaza-2′-deoxyadenosines in 45–85% yields. The nucleosides were converted to 5′-O-mono-(dASRMP) or triphosphates (dASRTP) by phosphorylation. The modified triphosphates were also prepared by thiol addition to 7-vinyl-7-deaza-dATP. The triphosphates dASRTP were good substrates for DNA polymerases useful in the enzymatic Synthesis of base-modified oligoNucleotides (ONs) or DNA containing flexibly linked hydrophobic substituents in the major groove. Primer extension was used for the Synthesis of ONs with one or several modifications, PCR was used for the Synthesis of heavily modified DNA, whereas terminal deoxynucleotidyl transferase was used for a single-nucleotide labeling of the 3′-end.
Benjamin U Kaupp - One of the best experts on this subject based on the ideXlab platform.
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7 dialkylamino coumarin 4 yl methyl caged compounds as ultrafast and effective long wavelength phototriggers of 8bromo substituted cyclic Nucleotides
ChemBioChem, 2003Co-Authors: Volker Hagen, Stephan Frings, Burkhard Wiesner, Siegrun Helm, Benjamin U Kaupp, Jurgen BendigAbstract:[7-(Dimethylamino)coumarin-4-yl]methyl (DMACM) and [7-(diethylamino)coumarin-4-yl]methyl (DEACM) esters of 8-bromoadenosine 3',5'-cyclic monophosphate (8-Br-cAMP) and 8-bromoguanosine 3',5'-cyclic monophosphate (8-Br-cGMP) are described as novel caged compounds for 8-bromo-substituted cyclic Nucleotides. Synthesis is accomplished by treatment of the free acids of the cyclic Nucleotides with the corresponding 7(dialkylamino)-substituted 4(diazomethyl)coumarins. Irradiation of the DMACM- and DEACM-caged cyclic Nucleotides with UV light stimulates the release of the cyclic Nucleotides within roughly a nanosecond. The new caged compounds are resistant to hydrolysis in aqueous buffers and exhibit long-wavelength absorption properties with maxima at 400 nm, high extinction coefficients, and high quantum yields (0.15-0.31). Their favorable properties render these compounds the most efficient and rapid phototriggers of 8-bromo-substituted cyclic Nucleotides known. The usefulness of the compounds for physiological studies under nondamaging light conditions was examined in HEK293 cells expressing the alpha subunit of the cyclic-nucleotide-gated (CNG) channel of cone photoreceptors (CNGA3) and of olfactory neurons (CNGA2) by using confocal laser scanning microscopy and the patch clamp technique.
