The Experts below are selected from a list of 85536 Experts worldwide ranked by ideXlab platform
Jong Chul Rhee - One of the best experts on this subject based on the ideXlab platform.
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high expression of intestinal Type mucin muc2 in intraductal papillary mucinous neoplasms coexisting with extrapancreatic gastrointestinal cancers
Pancreas, 2006Co-Authors: Sunyoung Lee, Dong Wook Choi, Keetaek Jang, Kyu Taek Lee, Seongho Choi, Jin Seok Heo, Jong Kyun Lee, Seung Woon Paik, Jong Chul RheeAbstract:Objectives: It is known that intraductal papillary mucinous neoplasm (IPMN) is associated with a high incidence of extrapancreatic neoplasms. We tried to uncover the characteristics and gene expressions of IPMNs that coexist with other extrapancreatic gastrointestinal cancers. Methods: We retrieved the surgical specimens from 54 IPMN patients, including 7 cases of IPMNs that coexisted with extrapancreatic gastrointestinal cancers. The immunohistochemical staining (p21, Bcl-2, p53, intestinal-Type secretory mucin [MUC2], and MUC5AC) and the pathological subTypes of the tumor papillae (the intestinal Type, pancreaticobiliary Type, Null Type, or unclassified Type) were analyzed. Results: MUC2 expression was noticed more frequently in the IPMN coexisting with extrapancreatic cancers (6/7) than in the IPMN without extrapancreatic cancers (18/47; P = 0.04). There were no differences in p21 (P = 0.12), p53 (P = 0.25), and MUC5AC (P = 1.0) expressions between the 2 groups. IPMN with extrapancreatic cancers was noticed in all subTypes of papillae. Conclusions: Regardless of the subTypes of papillae, transcription of the MUC2 might be related with the synchronous extrapancreatic gastrointestinal cancer development that is seen with IPMN. Therefore, careful systemic surveillance is needed to detect coexisting gastrointestinal cancer for all the subTypes of IPMN with MUC2 expression.
David S Klimstra - One of the best experts on this subject based on the ideXlab platform.
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pathologically and biologically distinct Types of epithelium in intraductal papillary mucinous neoplasms delineation of an intestinal pathway of carcinogenesis in the pancreas
The American Journal of Surgical Pathology, 2004Co-Authors: Volkan N Adsay, Kambiz Merati, Fazlul H Sarkar, Ralph H Hruban, Edi Levi, Olca Basturk, Christine A Iacobuziodonahue, Jeanette D Cheng, David S KlimstraAbstract:Although general characteristics of intraductal papillary mucinous neoplasms (IPMNs) and their delineation from other pancreatic tumors have been well established, several issues regarding their biology and management remain unresolved. It has been noted briefly by us and other authors that there are different Types of papillae in IPMNs; however, their frequency, biologic significance, and clinical relevance are unknown. In this study, the association of different papillary patterns with clinical, pathologic, and biologic parameters was studied in 74 IPMNs, and the expression profile of CDX2 (a specific marker and one of the key determinants of intestinal "programming," and a tumor suppressor) was determined immunohistochemically in addition to MUC1 (a marker of an "aggressive" phenoType in pancreatic neoplasia) and MUC2 ("intestinal Type mucin," a marker of the "indolent" phenoType, and a tumor suppressor). The patterns of papillae identified and their association with these parameters were as follows: 1) The intestinal-Type (Yonezawa's dark-cell Type), similar to villous adenomas, was seen in 26 of 74 (35%) cases. The majority harbored carcinoma in situ (85%) or borderline atypia (15%). They tended to be large (mean, 5.5 cm). Most expressed CDX2 (95%) and MUC2 (92%) but not MUC1 (8%). This Type was more commonly associated with colloid-Type invasion (14 of 16 invasive carcinomas were of colloid Type). 2) The pancreatobiliary Type, characterized by arborizing papillae lined by cuboidal cells resembling papillary neoplasms of the biliary tract, was present in 22% of the cases. These were mostly graded as carcinoma in situ (94%); they rarely expressed CDX2 (6%) or MUC2 (19%) but often showed MUC1 labeling (44%). This pattern was more commonly associated with the tubular Type of invasive carcinoma and had a slight tendency for a more aggressive clinical course. 3) The Null Type was characterized by abundant apical mucin and basally located nuclei, similar to the gastric foveolar epithelium. Thirty-one percent of IPMNs had this Type of papillae, but this pattern was also present in the background of other IPMNs and in the cystic components of most cases as well. Most pure Null-Type IPMNs were devoid of complexity and consequently classified as adenoma (48%). They tended to be small (mean, 2.6 cm), were often negative for CDX2, MUC1, and MUC2, and were rarely associated with invasive carcinoma. 4) Some IPMNs (12%) exhibited features that were difficult to classify, and 2 cases had a mixture of pancreatobiliary and intestinal Types of papillae. In conclusion, IPMNs include pathologically and biologically distinct epithelial patterns. CDX2 and MUC2 expression is relatively specific for the intestinal Type papillae, confirming that these IPMNs indeed exhibit intestinal differentiation. Their close association with colloid carcinoma, which also shows consistent MUC2 and CDX2 expression, supports the existence of an intestinal pathway of carcinogenesis. This "metaplastic" pathway may reflect different genetic events in the development of these IPMNs, and the presence of intestinal differentiation may potentially be used in prognostication and stratification of patients into appropriate treatment categories.
Parikshaa Gupta - One of the best experts on this subject based on the ideXlab platform.
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morphologic and immunocytochemical features of high grade serous carcinoma of ovary in ascitic fluid effusion and fine needle aspiration cytology
American Journal of Clinical Pathology, 2020Co-Authors: Akriti Bansal, Radhika Srinivasan, Manish Rohilla, Archana Sundaram, Arvind Rajwanshi, Vanita Suri, Subhash Chandra Saha, Nalini Gupta, Parikshaa GuptaAbstract:OBJECTIVES: High-grade serous carcinoma (HGSC) is the most common ovarian malignancy. The role of cytopathology in obtaining tissue diagnosis before institution of neoadjuvant chemotherapy (NACT) was evaluated. METHODS: All histopathology-proven HGSC specimens between 2015 and 2018 with prior cytopathologic diagnosis by ascitic fluid evaluation or fine-needle aspiration (FNA) of ovarian mass were reviewed with cell block immunocytochemistry for CK7, CK20, PAX8, WT1, and p53. RESULTS: Of 288 cases of HGSC, pre-NACT cytology diagnosis was established in 32% (93/288), with specific HGSC diagnoses made on ascitic fluid in 88% (82/93) and by ovarian mass FNA in 12% (11/93). The ascitic fluid showed moderate/high cellularity with papillary clusters in 76% (71/93) cases. Cell block immunocytochemistry showed tumor cells positive for CK7, PAX8, and WT1. p53 showed mutant or Null-Type positivity in 65% (33/51) and 33% (17/51) of cases, respectively, with 100% concordance with subsequent histopathology specimens. Poor/intermediate response to chemotherapy was shown in 75% of cases. CONCLUSIONS: Combined assessment of cytomorphology, cell block histomorphology, and ancillary immunohistochemical testing, including PAX8, WT1, and p53, allows for specific pre-NACT diagnoses of HGSC in ascitic fluid and ovarian FNA cytology. This practice allows for initiation of chemotherapy and diminution of disease burden prior to definitive surgical therapy.
Sunyoung Lee - One of the best experts on this subject based on the ideXlab platform.
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high expression of intestinal Type mucin muc2 in intraductal papillary mucinous neoplasms coexisting with extrapancreatic gastrointestinal cancers
Pancreas, 2006Co-Authors: Sunyoung Lee, Dong Wook Choi, Keetaek Jang, Kyu Taek Lee, Seongho Choi, Jin Seok Heo, Jong Kyun Lee, Seung Woon Paik, Jong Chul RheeAbstract:Objectives: It is known that intraductal papillary mucinous neoplasm (IPMN) is associated with a high incidence of extrapancreatic neoplasms. We tried to uncover the characteristics and gene expressions of IPMNs that coexist with other extrapancreatic gastrointestinal cancers. Methods: We retrieved the surgical specimens from 54 IPMN patients, including 7 cases of IPMNs that coexisted with extrapancreatic gastrointestinal cancers. The immunohistochemical staining (p21, Bcl-2, p53, intestinal-Type secretory mucin [MUC2], and MUC5AC) and the pathological subTypes of the tumor papillae (the intestinal Type, pancreaticobiliary Type, Null Type, or unclassified Type) were analyzed. Results: MUC2 expression was noticed more frequently in the IPMN coexisting with extrapancreatic cancers (6/7) than in the IPMN without extrapancreatic cancers (18/47; P = 0.04). There were no differences in p21 (P = 0.12), p53 (P = 0.25), and MUC5AC (P = 1.0) expressions between the 2 groups. IPMN with extrapancreatic cancers was noticed in all subTypes of papillae. Conclusions: Regardless of the subTypes of papillae, transcription of the MUC2 might be related with the synchronous extrapancreatic gastrointestinal cancer development that is seen with IPMN. Therefore, careful systemic surveillance is needed to detect coexisting gastrointestinal cancer for all the subTypes of IPMN with MUC2 expression.
Ryuichi Yatani - One of the best experts on this subject based on the ideXlab platform.
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genetic polymorphisms in cytochrome p450 cyp 1a1 cyp1a2 cyp2e1 glutathione s transferase gst m1 and gstt1 and susceptibility to prostate cancer in the japanese population
Cancer Letters, 2001Co-Authors: Mariko Murata, Masatoshi Watanabe, Mikio Yamanaka, Yoshinobu Kubota, Haruo Ito, Minako Nagao, Takahiko Katoh, Tetsuya Kamataki, Juichi Kawamura, Ryuichi YataniAbstract:Abstract Associations between genetic polymorphisms of CYP1A1, CYP1A2, CYP2E1, GSTM1 and GSTT1 and prostate cancer (PCa) were analyzed in a case-control study of 315 individuals. The frequency of valine (Val)/valine (Val) genoTypes for CYP1A1 was 11.3% in cases compared with 5.5% in controls, this polymorphism thus being associated with a significantly increased risk of PCa (odds ratio=2.4, 95% confidence interval (CI)=1.01–5.57). No links were detected between PCa and polymorphisms in other enzymes. However, the combination of CYP1A1 (Ile/Val and/or Val/Val) polymorphisms with the GSTM1 Null Type resulted in an OR of 2.2 (CI=1.10–4.57, 1.12–4.20, respectively). This study suggests that the CYP1A1 polymorphism and its combination with GSTM1 may be associated with PCa susceptibility in the Japanese population.
