The Experts below are selected from a list of 5889 Experts worldwide ranked by ideXlab platform
Xosé Luis Otero - One of the best experts on this subject based on the ideXlab platform.
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soil processes and Nutrient Bioavailability in the rhizosphere of bolax gummifera in a subantarctic environment martial mountains ushuaia argentina
Catena, 2015Co-Authors: Xosé Luis Otero, A Gonzalezguzman, Valdomiro Severino De Souzajunior, Augusto Perezalberti, Felipe MacíasAbstract:Abstract The aim of this study was to explore the effects of plants on the underlying substrate and the possible environmental implications of such effects in an extreme environment. For this purpose, we sampled the rhizosphere soil of Bolax gummifera , which is one of the most characteristic vascular plants in the Martial Mountains (Tierra del Fuego, Argentina) where plant cover is typically sparse. Superficial (0–5 cm, Rh5) and subsuperficial (5–15 cm, Rh15) samples of B. gummifera rhizosphere and bulk soil were collected from a small homogeneous area, and the following parameters were analyzed: particle size, pH, total organic carbon (TOC), total nitrogen (TN), total Fe (TFe), Fe and Al associated with organic matter (soluble in sodium pyrophosphate, C pyro ), amorphous Fe oxyhydroxides (extracted with ammonium oxalate, Fe oxa ), crystalline Fe oxyhydroxides (extracted with sodium dithionite, Fe dit ), bioavailable plant Nutrients (soluble in Mehlich 3 extractant) and clay mineralogy. Several differences were found between rhizosphere soil (RS) and bulk soil. The TOC was 8 times higher and the TN and C pyro were 4 times higher in the rhizosphere than in the bulk soil. Mineralogical results suggest a slightly higher degree of chemical weathering (mainly affecting biotite and amphibole minerals) in the RS than in bulk soil, consistent with significantly higher Nutrient Bioavailability in RS (Ca, Mg, K, Co, and Fe). On the other hand, the higher concentrations of C and metals extracted with sodium pyrophosphate suggest the formation of more metal–organic complexes in the rhizosphere than in the bulk soil. This metal fraction is considered as readily mobilizable and available and can be leached from the soil to continental and coastal waters. This effect will be enhanced in cold climates by expansion of plant cover due to the increased temperature expected under climate change.
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Soil processes and Nutrient Bioavailability in the rhizosphere of Bolax gummifera in a subantarctic environment (Martial Mountains, Ushuaia—Argentina)
CATENA, 2015Co-Authors: Xosé Luis Otero, A. González-guzman, Valdomiro Severino De Souza-júnior, Augusto Pérez-alberti, Felipe MacíasAbstract:Abstract The aim of this study was to explore the effects of plants on the underlying substrate and the possible environmental implications of such effects in an extreme environment. For this purpose, we sampled the rhizosphere soil of Bolax gummifera , which is one of the most characteristic vascular plants in the Martial Mountains (Tierra del Fuego, Argentina) where plant cover is typically sparse. Superficial (0–5 cm, Rh5) and subsuperficial (5–15 cm, Rh15) samples of B. gummifera rhizosphere and bulk soil were collected from a small homogeneous area, and the following parameters were analyzed: particle size, pH, total organic carbon (TOC), total nitrogen (TN), total Fe (TFe), Fe and Al associated with organic matter (soluble in sodium pyrophosphate, C pyro ), amorphous Fe oxyhydroxides (extracted with ammonium oxalate, Fe oxa ), crystalline Fe oxyhydroxides (extracted with sodium dithionite, Fe dit ), bioavailable plant Nutrients (soluble in Mehlich 3 extractant) and clay mineralogy. Several differences were found between rhizosphere soil (RS) and bulk soil. The TOC was 8 times higher and the TN and C pyro were 4 times higher in the rhizosphere than in the bulk soil. Mineralogical results suggest a slightly higher degree of chemical weathering (mainly affecting biotite and amphibole minerals) in the RS than in bulk soil, consistent with significantly higher Nutrient Bioavailability in RS (Ca, Mg, K, Co, and Fe). On the other hand, the higher concentrations of C and metals extracted with sodium pyrophosphate suggest the formation of more metal–organic complexes in the rhizosphere than in the bulk soil. This metal fraction is considered as readily mobilizable and available and can be leached from the soil to continental and coastal waters. This effect will be enhanced in cold climates by expansion of plant cover due to the increased temperature expected under climate change.
Felipe Macías - One of the best experts on this subject based on the ideXlab platform.
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soil processes and Nutrient Bioavailability in the rhizosphere of bolax gummifera in a subantarctic environment martial mountains ushuaia argentina
Catena, 2015Co-Authors: Xosé Luis Otero, A Gonzalezguzman, Valdomiro Severino De Souzajunior, Augusto Perezalberti, Felipe MacíasAbstract:Abstract The aim of this study was to explore the effects of plants on the underlying substrate and the possible environmental implications of such effects in an extreme environment. For this purpose, we sampled the rhizosphere soil of Bolax gummifera , which is one of the most characteristic vascular plants in the Martial Mountains (Tierra del Fuego, Argentina) where plant cover is typically sparse. Superficial (0–5 cm, Rh5) and subsuperficial (5–15 cm, Rh15) samples of B. gummifera rhizosphere and bulk soil were collected from a small homogeneous area, and the following parameters were analyzed: particle size, pH, total organic carbon (TOC), total nitrogen (TN), total Fe (TFe), Fe and Al associated with organic matter (soluble in sodium pyrophosphate, C pyro ), amorphous Fe oxyhydroxides (extracted with ammonium oxalate, Fe oxa ), crystalline Fe oxyhydroxides (extracted with sodium dithionite, Fe dit ), bioavailable plant Nutrients (soluble in Mehlich 3 extractant) and clay mineralogy. Several differences were found between rhizosphere soil (RS) and bulk soil. The TOC was 8 times higher and the TN and C pyro were 4 times higher in the rhizosphere than in the bulk soil. Mineralogical results suggest a slightly higher degree of chemical weathering (mainly affecting biotite and amphibole minerals) in the RS than in bulk soil, consistent with significantly higher Nutrient Bioavailability in RS (Ca, Mg, K, Co, and Fe). On the other hand, the higher concentrations of C and metals extracted with sodium pyrophosphate suggest the formation of more metal–organic complexes in the rhizosphere than in the bulk soil. This metal fraction is considered as readily mobilizable and available and can be leached from the soil to continental and coastal waters. This effect will be enhanced in cold climates by expansion of plant cover due to the increased temperature expected under climate change.
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Soil processes and Nutrient Bioavailability in the rhizosphere of Bolax gummifera in a subantarctic environment (Martial Mountains, Ushuaia—Argentina)
CATENA, 2015Co-Authors: Xosé Luis Otero, A. González-guzman, Valdomiro Severino De Souza-júnior, Augusto Pérez-alberti, Felipe MacíasAbstract:Abstract The aim of this study was to explore the effects of plants on the underlying substrate and the possible environmental implications of such effects in an extreme environment. For this purpose, we sampled the rhizosphere soil of Bolax gummifera , which is one of the most characteristic vascular plants in the Martial Mountains (Tierra del Fuego, Argentina) where plant cover is typically sparse. Superficial (0–5 cm, Rh5) and subsuperficial (5–15 cm, Rh15) samples of B. gummifera rhizosphere and bulk soil were collected from a small homogeneous area, and the following parameters were analyzed: particle size, pH, total organic carbon (TOC), total nitrogen (TN), total Fe (TFe), Fe and Al associated with organic matter (soluble in sodium pyrophosphate, C pyro ), amorphous Fe oxyhydroxides (extracted with ammonium oxalate, Fe oxa ), crystalline Fe oxyhydroxides (extracted with sodium dithionite, Fe dit ), bioavailable plant Nutrients (soluble in Mehlich 3 extractant) and clay mineralogy. Several differences were found between rhizosphere soil (RS) and bulk soil. The TOC was 8 times higher and the TN and C pyro were 4 times higher in the rhizosphere than in the bulk soil. Mineralogical results suggest a slightly higher degree of chemical weathering (mainly affecting biotite and amphibole minerals) in the RS than in bulk soil, consistent with significantly higher Nutrient Bioavailability in RS (Ca, Mg, K, Co, and Fe). On the other hand, the higher concentrations of C and metals extracted with sodium pyrophosphate suggest the formation of more metal–organic complexes in the rhizosphere than in the bulk soil. This metal fraction is considered as readily mobilizable and available and can be leached from the soil to continental and coastal waters. This effect will be enhanced in cold climates by expansion of plant cover due to the increased temperature expected under climate change.
Bin Yao - One of the best experts on this subject based on the ideXlab platform.
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Expression of an extremely acidic β-1,4-glucanase from thermoacidophilic Alicyclobacillus sp. A4 in Pichia pastoris is improved by truncating the gene sequence
Microbial cell factories, 2010Co-Authors: Yingguo Bai, Jianshe Wang, Zhifang Zhang, Pengjun Shi, Huiying Luo, Huoqing Huang, Chunliang Luo, Bin YaoAbstract:Background Alicyclobacillus sp. A4 is thermoacidophilic and produces many glycoside hydrolases. An extremely acidic β-1,4-glucanase (CelA4) has been isolated from Alicyclobacillus sp. A4 and purified. This glucanase with a molecular mass of 48.6 kDa decreases the viscosity of barley-soybean feed under simulated gastric conditions. Therefore, it has the potential to improve the Nutrient Bioavailability of pig feed. For the study reported herein, the full-length gene, CelA4, of this glucanase (CelA4) was identified using the sequences of six peptides and cloned from strain A4. The gene fragment (CelA4F) encoding the mature protein was expressed in Pichia pastoris. Sequence truncation and glycosylation were found for recombinant CelA4F, both of which affected the expression efficiency. The physical properties of various forms of CelA4 as they affected enzymatic activity were characterized.
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from thermoacidophilic Alicyclobacillus sp. A4 in Pichia pastoris is improved by truncating the gene sequence
2010Co-Authors: Yingguo Bai, Jianshe Wang, Zhifang Zhang, Pengjun Shi, Huiying Luo, Huoqing Huang, Chunliang Luo, Bin YaoAbstract:Background: Alicyclobacillus sp. A4 is thermoacidophilic and produces many glycoside hydrolases. An extremely acidic β-1,4-glucanase (CelA4) has been isolated from Alicyclobacillus sp. A4 and purified. This glucanase with a molecular mass of 48.6 kDa decreases the viscosity of barley-soybean feed under simulated gastric conditions. Therefore, it has the potential to improve the Nutrient Bioavailability of pig feed. For the study reported herein, the full-length gene, CelA4, of this glucanase (CelA4) was identified using the sequences of six peptides and cloned from strain A4. The gene fragment (CelA4F) encoding the mature protein was expressed in Pichia pastoris. Sequence truncation and glycosylation were found for recombinant CelA4 F , both of which affected the expression efficiency. The physical
Yingguo Bai - One of the best experts on this subject based on the ideXlab platform.
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Expression of an extremely acidic β-1,4-glucanase from thermoacidophilic Alicyclobacillus sp. A4 in Pichia pastoris is improved by truncating the gene sequence
Microbial cell factories, 2010Co-Authors: Yingguo Bai, Jianshe Wang, Zhifang Zhang, Pengjun Shi, Huiying Luo, Huoqing Huang, Chunliang Luo, Bin YaoAbstract:Background Alicyclobacillus sp. A4 is thermoacidophilic and produces many glycoside hydrolases. An extremely acidic β-1,4-glucanase (CelA4) has been isolated from Alicyclobacillus sp. A4 and purified. This glucanase with a molecular mass of 48.6 kDa decreases the viscosity of barley-soybean feed under simulated gastric conditions. Therefore, it has the potential to improve the Nutrient Bioavailability of pig feed. For the study reported herein, the full-length gene, CelA4, of this glucanase (CelA4) was identified using the sequences of six peptides and cloned from strain A4. The gene fragment (CelA4F) encoding the mature protein was expressed in Pichia pastoris. Sequence truncation and glycosylation were found for recombinant CelA4F, both of which affected the expression efficiency. The physical properties of various forms of CelA4 as they affected enzymatic activity were characterized.
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from thermoacidophilic Alicyclobacillus sp. A4 in Pichia pastoris is improved by truncating the gene sequence
2010Co-Authors: Yingguo Bai, Jianshe Wang, Zhifang Zhang, Pengjun Shi, Huiying Luo, Huoqing Huang, Chunliang Luo, Bin YaoAbstract:Background: Alicyclobacillus sp. A4 is thermoacidophilic and produces many glycoside hydrolases. An extremely acidic β-1,4-glucanase (CelA4) has been isolated from Alicyclobacillus sp. A4 and purified. This glucanase with a molecular mass of 48.6 kDa decreases the viscosity of barley-soybean feed under simulated gastric conditions. Therefore, it has the potential to improve the Nutrient Bioavailability of pig feed. For the study reported herein, the full-length gene, CelA4, of this glucanase (CelA4) was identified using the sequences of six peptides and cloned from strain A4. The gene fragment (CelA4F) encoding the mature protein was expressed in Pichia pastoris. Sequence truncation and glycosylation were found for recombinant CelA4 F , both of which affected the expression efficiency. The physical
Huoqing Huang - One of the best experts on this subject based on the ideXlab platform.
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Expression of an extremely acidic β-1,4-glucanase from thermoacidophilic Alicyclobacillus sp. A4 in Pichia pastoris is improved by truncating the gene sequence
Microbial cell factories, 2010Co-Authors: Yingguo Bai, Jianshe Wang, Zhifang Zhang, Pengjun Shi, Huiying Luo, Huoqing Huang, Chunliang Luo, Bin YaoAbstract:Background Alicyclobacillus sp. A4 is thermoacidophilic and produces many glycoside hydrolases. An extremely acidic β-1,4-glucanase (CelA4) has been isolated from Alicyclobacillus sp. A4 and purified. This glucanase with a molecular mass of 48.6 kDa decreases the viscosity of barley-soybean feed under simulated gastric conditions. Therefore, it has the potential to improve the Nutrient Bioavailability of pig feed. For the study reported herein, the full-length gene, CelA4, of this glucanase (CelA4) was identified using the sequences of six peptides and cloned from strain A4. The gene fragment (CelA4F) encoding the mature protein was expressed in Pichia pastoris. Sequence truncation and glycosylation were found for recombinant CelA4F, both of which affected the expression efficiency. The physical properties of various forms of CelA4 as they affected enzymatic activity were characterized.
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from thermoacidophilic Alicyclobacillus sp. A4 in Pichia pastoris is improved by truncating the gene sequence
2010Co-Authors: Yingguo Bai, Jianshe Wang, Zhifang Zhang, Pengjun Shi, Huiying Luo, Huoqing Huang, Chunliang Luo, Bin YaoAbstract:Background: Alicyclobacillus sp. A4 is thermoacidophilic and produces many glycoside hydrolases. An extremely acidic β-1,4-glucanase (CelA4) has been isolated from Alicyclobacillus sp. A4 and purified. This glucanase with a molecular mass of 48.6 kDa decreases the viscosity of barley-soybean feed under simulated gastric conditions. Therefore, it has the potential to improve the Nutrient Bioavailability of pig feed. For the study reported herein, the full-length gene, CelA4, of this glucanase (CelA4) was identified using the sequences of six peptides and cloned from strain A4. The gene fragment (CelA4F) encoding the mature protein was expressed in Pichia pastoris. Sequence truncation and glycosylation were found for recombinant CelA4 F , both of which affected the expression efficiency. The physical
