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Barbara Jane Christ - One of the best experts on this subject based on the ideXlab platform.

  • in vitro culture of the Obligate Parasite spongospora subterranea cercozoa plasmodiophorida associated with root inducing transferred dna transformed potato hairy roots
    Journal of Eukaryotic Microbiology, 2007
    Co-Authors: Xinshun Qu, Barbara Jane Christ
    Abstract:

    . Spongospora subterranea is a soil-borne, Obligate parasitic protist that causes powdery scab of potatoes. In this study, an in vitro culture system was developed for the maintenance and proliferation of the protist in potato hairy roots. The hairy roots of potato were induced in vitro with Agrobacterium rhizogenes. Cystosori of S. subterranea from potato scab lesions were surface disinfested and used to inoculate potato hairy roots. Plasmodia, zoosporangia, and cystosori were observed microscopically in the hairy roots within 6 wk after inoculation, indicating the completion of the life cycle of S. subterranea in vitro. This is the first in vitro culture system for S. subterranea, and will be a valuable tool to study fundamental and practical aspects of the biology of the Parasite.

  • In Vitro Culture of the Obligate Parasite Spongospora subterranea (Cercozoa; Plasmodiophorida) Associated with Root‐Inducing Transferred‐DNA Transformed Potato Hairy Roots
    Journal of Eukaryotic Microbiology, 2007
    Co-Authors: Xinshun Qu, Barbara Jane Christ
    Abstract:

    . Spongospora subterranea is a soil-borne, Obligate parasitic protist that causes powdery scab of potatoes. In this study, an in vitro culture system was developed for the maintenance and proliferation of the protist in potato hairy roots. The hairy roots of potato were induced in vitro with Agrobacterium rhizogenes. Cystosori of S. subterranea from potato scab lesions were surface disinfested and used to inoculate potato hairy roots. Plasmodia, zoosporangia, and cystosori were observed microscopically in the hairy roots within 6 wk after inoculation, indicating the completion of the life cycle of S. subterranea in vitro. This is the first in vitro culture system for S. subterranea, and will be a valuable tool to study fundamental and practical aspects of the biology of the Parasite.

Xinshun Qu - One of the best experts on this subject based on the ideXlab platform.

  • in vitro culture of the Obligate Parasite spongospora subterranea cercozoa plasmodiophorida associated with root inducing transferred dna transformed potato hairy roots
    Journal of Eukaryotic Microbiology, 2007
    Co-Authors: Xinshun Qu, Barbara Jane Christ
    Abstract:

    . Spongospora subterranea is a soil-borne, Obligate parasitic protist that causes powdery scab of potatoes. In this study, an in vitro culture system was developed for the maintenance and proliferation of the protist in potato hairy roots. The hairy roots of potato were induced in vitro with Agrobacterium rhizogenes. Cystosori of S. subterranea from potato scab lesions were surface disinfested and used to inoculate potato hairy roots. Plasmodia, zoosporangia, and cystosori were observed microscopically in the hairy roots within 6 wk after inoculation, indicating the completion of the life cycle of S. subterranea in vitro. This is the first in vitro culture system for S. subterranea, and will be a valuable tool to study fundamental and practical aspects of the biology of the Parasite.

  • In Vitro Culture of the Obligate Parasite Spongospora subterranea (Cercozoa; Plasmodiophorida) Associated with Root‐Inducing Transferred‐DNA Transformed Potato Hairy Roots
    Journal of Eukaryotic Microbiology, 2007
    Co-Authors: Xinshun Qu, Barbara Jane Christ
    Abstract:

    . Spongospora subterranea is a soil-borne, Obligate parasitic protist that causes powdery scab of potatoes. In this study, an in vitro culture system was developed for the maintenance and proliferation of the protist in potato hairy roots. The hairy roots of potato were induced in vitro with Agrobacterium rhizogenes. Cystosori of S. subterranea from potato scab lesions were surface disinfested and used to inoculate potato hairy roots. Plasmodia, zoosporangia, and cystosori were observed microscopically in the hairy roots within 6 wk after inoculation, indicating the completion of the life cycle of S. subterranea in vitro. This is the first in vitro culture system for S. subterranea, and will be a valuable tool to study fundamental and practical aspects of the biology of the Parasite.

Renaud Ioos - One of the best experts on this subject based on the ideXlab platform.

  • An Optimized Duplex Real-Time PCR Tool for Sensitive Detection of the Quarantine Oomycete Plasmopara halstedii in Sunflower Seeds
    Phytopathology, 2016
    Co-Authors: Renaud Ioos, Celine Fourrier, Kathryn Webb, Veronique Wilson, Jean-Luc Schereffer, Denis Tourvieille De Labrouhe
    Abstract:

    Ioos, R., Fourrier, C., Wilson, V.. Webb, K., Schereffer, J.-L., and Tourvieille de Labrouhe, D. 2012. An optimized duplex real-time PCR tool for sensitive detection of the quarantine oomycete Plasmopara halstedii in sunflower seeds. Phytopathology 102:908-917. Plasmopara halstedii, the causal agent of downy mildew of sunflower, is an oomycete listed as a quarantine pathogen. This Obligate Parasite resides in a quiescent state in seeds of sunflower and can be spread from seed production areas to areas of crop production by international seed trade. To prevent the spread or the introduction of potentially new genotypes or fungicide-tolerant strains, an efficient method to detect P halstedii in sunflower seed is required. This work reports the optimization of a real-time detection tool that targets the pathogen within sunflower seeds, and provides statistically validated data for that tool. The tool proved to be specific and inclusive, based on computer simulation and in vitro assessments, and could detect as few as 45 copies of target DNA. A fully optimized DNA extraction protocol was also developed starting from a sample of 1,000 sunflower seeds, and enabled the detection of

  • an optimized duplex real time pcr tool for sensitive detection of the quarantine oomycete plasmopara halstedii in sunflower seeds
    Phytopathology, 2012
    Co-Authors: Renaud Ioos, Celine Fourrier, Kathryn Webb, Veronique Wilson, Jean-Luc Schereffer, Denis Tourvieille De Labrouhe
    Abstract:

    Plasmopara halstedii, the causal agent of downy mildew of sunflower, is an oomycete listed as a quarantine pathogen. This Obligate Parasite resides in a quiescent state in seeds of sunflower and can be spread from seed production areas to areas of crop production by international seed trade. To prevent the spread or the introduction of potentially new genotypes or fungicide-tolerant strains, an efficient method to detect P. halstedii in sunflower seed is required. This work reports the optimization of a real-time detection tool that targets the pathogen within sunflower seeds, and provides statistically validated data for that tool. The tool proved to be specific and inclusive, based on computer simulation and in vitro assessments, and could detect as few as 45 copies of target DNA. A fully optimized DNA extraction protocol was also developed starting from a sample of 1,000 sunflower seeds, and enabled the detection of <1 infected seed/1,000 seeds. To ensure reliability of the results, a set of controls was used systematically during the assays, including a plant-specific probe used in a duplex quantitative polymerase chain reaction that enabled the assessment of the quality of each DNA extract.

  • An optimized duplex real-time PCR tool for sensitive detection of the quarantine oomycete Plasmopara halstedii in sunflower seeds.
    Phytopathology, 2012
    Co-Authors: Renaud Ioos, Celine Fourrier, Kathryn Webb, Veronique Wilson, Jean-Luc Schereffer, Denis Tourvieille De Labrouhe
    Abstract:

    ABSTRACT Plasmopara halstedii, the causal agent of downy mildew of sunflower, is an oomycete listed as a quarantine pathogen. This Obligate Parasite resides in a quiescent state in seeds of sunflower and can be spread from seed production areas to areas of crop production by international seed trade. To prevent the spread or the introduction of potentially new genotypes or fungicide-tolerant strains, an efficient method to detect P. halstedii in sunflower seed is required. This work reports the optimization of a real-time detection tool that targets the pathogen within sunflower seeds, and provides statistically validated data for that tool. The tool proved to be specific and inclusive, based on computer simulation and in vitro assessments, and could detect as few as 45 copies of target DNA. A fully optimized DNA extraction protocol was also developed starting from a sample of 1,000 sunflower seeds, and enabled the detection of

Denis Tourvieille De Labrouhe - One of the best experts on this subject based on the ideXlab platform.

  • an optimized duplex real time pcr tool for sensitive detection of the quarantine oomycete plasmopara halstedii in sunflower seeds
    Phytopathology, 2012
    Co-Authors: Renaud Ioos, Celine Fourrier, Kathryn Webb, Veronique Wilson, Jean-Luc Schereffer, Denis Tourvieille De Labrouhe
    Abstract:

    Plasmopara halstedii, the causal agent of downy mildew of sunflower, is an oomycete listed as a quarantine pathogen. This Obligate Parasite resides in a quiescent state in seeds of sunflower and can be spread from seed production areas to areas of crop production by international seed trade. To prevent the spread or the introduction of potentially new genotypes or fungicide-tolerant strains, an efficient method to detect P. halstedii in sunflower seed is required. This work reports the optimization of a real-time detection tool that targets the pathogen within sunflower seeds, and provides statistically validated data for that tool. The tool proved to be specific and inclusive, based on computer simulation and in vitro assessments, and could detect as few as 45 copies of target DNA. A fully optimized DNA extraction protocol was also developed starting from a sample of 1,000 sunflower seeds, and enabled the detection of <1 infected seed/1,000 seeds. To ensure reliability of the results, a set of controls was used systematically during the assays, including a plant-specific probe used in a duplex quantitative polymerase chain reaction that enabled the assessment of the quality of each DNA extract.

  • An optimized duplex real-time PCR tool for sensitive detection of the quarantine oomycete Plasmopara halstedii in sunflower seeds.
    Phytopathology, 2012
    Co-Authors: Renaud Ioos, Celine Fourrier, Kathryn Webb, Veronique Wilson, Jean-Luc Schereffer, Denis Tourvieille De Labrouhe
    Abstract:

    ABSTRACT Plasmopara halstedii, the causal agent of downy mildew of sunflower, is an oomycete listed as a quarantine pathogen. This Obligate Parasite resides in a quiescent state in seeds of sunflower and can be spread from seed production areas to areas of crop production by international seed trade. To prevent the spread or the introduction of potentially new genotypes or fungicide-tolerant strains, an efficient method to detect P. halstedii in sunflower seed is required. This work reports the optimization of a real-time detection tool that targets the pathogen within sunflower seeds, and provides statistically validated data for that tool. The tool proved to be specific and inclusive, based on computer simulation and in vitro assessments, and could detect as few as 45 copies of target DNA. A fully optimized DNA extraction protocol was also developed starting from a sample of 1,000 sunflower seeds, and enabled the detection of

Denis Tourvieille De Labrouhe - One of the best experts on this subject based on the ideXlab platform.

  • An Optimized Duplex Real-Time PCR Tool for Sensitive Detection of the Quarantine Oomycete Plasmopara halstedii in Sunflower Seeds
    Phytopathology, 2016
    Co-Authors: Renaud Ioos, Celine Fourrier, Kathryn Webb, Veronique Wilson, Jean-Luc Schereffer, Denis Tourvieille De Labrouhe
    Abstract:

    Ioos, R., Fourrier, C., Wilson, V.. Webb, K., Schereffer, J.-L., and Tourvieille de Labrouhe, D. 2012. An optimized duplex real-time PCR tool for sensitive detection of the quarantine oomycete Plasmopara halstedii in sunflower seeds. Phytopathology 102:908-917. Plasmopara halstedii, the causal agent of downy mildew of sunflower, is an oomycete listed as a quarantine pathogen. This Obligate Parasite resides in a quiescent state in seeds of sunflower and can be spread from seed production areas to areas of crop production by international seed trade. To prevent the spread or the introduction of potentially new genotypes or fungicide-tolerant strains, an efficient method to detect P halstedii in sunflower seed is required. This work reports the optimization of a real-time detection tool that targets the pathogen within sunflower seeds, and provides statistically validated data for that tool. The tool proved to be specific and inclusive, based on computer simulation and in vitro assessments, and could detect as few as 45 copies of target DNA. A fully optimized DNA extraction protocol was also developed starting from a sample of 1,000 sunflower seeds, and enabled the detection of

  • Variation in form and size of Plasmopara halstedii (sunflower downy mildew) zoosporangia
    Mycological Progress, 2015
    Co-Authors: Nachaat Sakr, Mireille Ducher, Jeanne Tourvieille, Pascal Walser, Denis Tourvieille De Labrouhe
    Abstract:

    Zoosporangia form and size were studied on a collection of 94 strains of Plasmopara halstedii (sunflower downy mildew). Both oval and round forms were present in all strains analysed. The proportion of two forms varied significantly according to strain and plant age but more especially to host plant genotype. Whatever the strain or host genotype, oval zoosporangia were larger than round ones, but there was no relation between the proportion of the oval form and mean zoosporangia size. There was no relation between zoosporangia form or size and race virulence profiles or aggressiveness criteria, with the possible exception of zoosporangia size and sporulation density. It is concluded that, for this Obligate Parasite, although form and size of zoosporangia depend on pathogen strain, these characters also vary according to growth conditions of Plasmopara halstedii, in particular to the genotype of the plant host.