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Andreas F Widmer - One of the best experts on this subject based on the ideXlab platform.
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antimicrobial efficacy of 3 oral antiseptics containing Octenidine polyhexamethylene biguanide or citroxx can chlorhexidine be replaced
Infection Control and Hospital Epidemiology, 2010Co-Authors: Nadine Rohrer, Roland Weiger, Andreas F Widmer, Tuomas Waltimo, Eva M Kulik, Elisabeth Filipuzzijenny, Clemens WalterAbstract:background. Use of oral antiseptics decreases the bacterial load in the oral cavity. objective. To compare the antimicrobial activity of 3 novel oral antiseptics with that of chlorhexidine, which is considered the “gold standard” of oral hygiene. design. Comparative in vitro study. methods. Four common oral microorganisms (Streptococcus sanguinis, Streptococcus mutans, Candida albicans, and Fusobacterium nucleatum) were tested under standard conditions and at different concentrations, by use of a broth dilution assay and an agar diffusion assay and by calculating the log10 reduction factor (RF). The antimicrobial activity of each antiseptic was assessed by counting the difference in bacterial densities (ie, the log10 number of colony-forming units of bacteria) before and after the disinfection process. results. The oral antiseptics containing Octenidine (with an RF in the range of 7.1–8.24 CFU/mL) and polyhexamethylene biguanide (with an RF in the range of 7.1–8.24 CFU/mL) demonstrated antimicrobial activity comparable to that of chlorhexidine (with an RF in the range of 1.03–8.24 CFU/mL), whereas the mouth rinse containing Citroxx (Citroxx Biosciences; with an RF in the range of 0.22–1.36 CFU/mL) showed significantly weaker antimicrobial efficacy. Overall, Octenidine and polyhexamethylene biguanide were more active at lower concentrations. conclusion. Oral antiseptics containing the antimicrobial agent Octenidine or polyhexamethylene biguanide may be considered as potent alternatives to chlorhexidine-based preparations.
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skin disinfection with Octenidine dihydrochloride for central venous catheter site care a double blind randomized controlled trial
Clinical Microbiology and Infection, 2010Co-Authors: M Dettenkofer, C Wilson, Alois Gratwohl, Claudia Schmoor, Hartmut Bertz, Reno Frei, Dominik Heim, D Luft, S Schulz, Andreas F WidmerAbstract:Abstract To compare the efficacy of two commercially available, alcohol-based antiseptic solutions for preparation and care of central venous catheter (CVC) insertion sites, with and without Octenidine dihydrochloride, a double-blind, randomized, controlled trial was undertaken in the haematology units and in one surgical unit of two university hospitals. Adult patients with a non-tunnelled CVC were randomly assigned to two different skin disinfection regimens at the insertion site: 0.1% Octenidine with 30% 1-propanol and 45% 2-propanol, and as control 74% ethanol with 10% 2-propanol. Endpoints were (i) skin colonization at the insertion site; (ii) -positive culture from the catheter tip (≥15 CFU); and (iii) occurrence of CVC-associated bloodstream infection (defined according to criteria set by the CDC). Four hundred patients with inserted CVC were enrolled from May 2002 through April 2005. Both groups were similar in respect of patient characteristics and co-morbidities. Skin colonization at the CVC insertion site during the first 10 days was significantly reduced by Octenidine treatment (relative difference Octenidine vs. control: 0.21; 95%CI: 0.11–0.39, p
Axel Kramer - One of the best experts on this subject based on the ideXlab platform.
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combined antibacterial effects of tissue tolerable plasma and a modern conventional liquid antiseptic on chronic wound treatment
Journal of Biophotonics, 2015Co-Authors: Martin Klebes, Christin Ulrich, Franziska Kluschke, Alexa Patzelt, Staffan Vandersee, H Richter, Adrienne Bob, Johanna Von Hutten, Jorien Tannette Krediet, Axel KramerAbstract:Potential antimicrobial effects of sequential applications of tissue-tolerable plasma (TTP) and the conventional liquid antiseptic Octenidine dihydrochloride (ODC) were investigated. 34 patients with chronic leg ulcers were treated with TTP, ODC or a combination of both. The bacterial colonization was measured semi-quantitatively before and immediately after treatment and changes in the microbial strains' compositions before and after antiseptic treatments were analyzed. All antiseptic procedures reduced the bacterial counts significantly. The sequential application of TTP and ODC displayed the highest antimicrobial efficacy. Me combined use of TTP and conventional antiseptics might represent the most efficient strategy for antiseptic treatment of chronic wounds.
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Comparison of the Antiseptic Efficacy of Tissue-Tolerable Plasma and an Octenidine Hydrochloride-Based Wound Antiseptic on Human Skin
Skin pharmacology and physiology, 2012Co-Authors: Juergen Lademann, Alexa Patzelt, Axel Kramer, Heike Richter, S. Schanzer, Gisela Thiede, K-d Weltmann, B. Hartmann, B. Lange-asschenfeldtAbstract:Colonization and infection of wounds represent a major reason for the impairment of tissue repair. Recently, it has been reported that tissue-tolerable plasma (TTP) is highly efficient in the reduction of the bacterial load of the skin. In the present study, the antiseptic efficacy of TTP was compared to that of Octenidine hydrochloride with 2-phenoxyethanol. Both antiseptic methods proved to be highly efficient. Cutaneous treatment of the skin with Octenidine hydrochloride and 2-phenoxyethanol leads to a 99% elimination of the bacteria, and 74% elimination is achieved by TTP treatment. Technical challenges with an early prototype TTP device could be held responsible for the slightly reduced antiseptic properties of TTP, compared to a standard antiseptic solution, since the manual treatment of the skin surface with a small beam of the TTP device might have led to an incomplete coverage of the treated area.
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standardized comparison of antiseptic efficacy of triclosan pvp iodine Octenidine dihydrochloride polyhexanide and chlorhexidine digluconate
Journal of Antimicrobial Chemotherapy, 2010Co-Authors: Torsten Koburger, J Siebert, Nilsolaf Hubner, M Braun, Axel KramerAbstract:Background This study presents a comparative investigation of the antimicrobial efficacy of the antiseptics PVP-iodine, triclosan, chlorhexidine, Octenidine and polyhexanide used for pre-surgical antisepsis and antiseptic treatment of skin, wounds and mucous membranes based on internationally accepted standards. Methods MICs and MBCs were determined in accordance with DIN 58940-7 and 58940-8 using Staphylococcus aureus (including methicillin-resistant Staphylococcus aureus), Enterococcus faecalis (including vancomycin-resistant Enterococcus), Streptococcus pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Clostridium perfringens, Haemophilus influenzae and Candida albicans. The microbicidal efficacy was determined in accordance with DIN EN 1040 and 1275 using S. aureus, P. aeruginosa and C. albicans. Results For chlorhexidine, Octenidine and polyhexanide, MIC(48) and MBC(24) ranged from 16 to 32 mg/L. Maximum values for triclosan ranged from 256 to 512 mg/L, with an efficacy gap against P. aeruginosa, while the maximum values of PVP-iodine were 1024 mg/L, with a gap against S. pneumoniae. Comparing the minimal effective concentrations, Octenidine was most effective. After 1 min, only Octenidine and PVP-iodine fulfil the requirements for antiseptics. Conclusions Tests under standardized and harmonized conditions help to choose the most efficacious agent. When a prolonged contact time is feasible, ranking of agents would be polyhexanide = Octenidine > chlorhexidine > triclosan > PVP-iodine. This is consistent with the recommendations for antisepsis of acute wounds. Polyhexanide seems to be preferable for chronic wounds due to its higher tolerability. If an immediate effect is required, ranking would be Octenidine = PVP-iodine>> polyhexanide > chlorhexidine > triclosan.
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Octenidine dihydrochloride a modern antiseptic for skin mucous membranes and wounds
Skin Pharmacology and Physiology, 2010Co-Authors: N O Hubner, J Siebert, Axel KramerAbstract:Octenidine dihydrochloride (Octenidine) was introduced for skin, mucous membrane and wound antisepsis more than 20 years ago. Until now, a wealth of knowledge has been gained, including in vitro and animal studies on efficacy, tolerance, safety and clinical experience both from case reports and prospective controlled trials. Nowadays, Octenidine is an established antiseptic in a large field of applications and represents an alternative to older substances such as chlorhexidine, polyvidone-iodine or triclosan. The review is based on the current literature and unpublished original data as well.
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influence of the antiseptic agents polyhexanide and Octenidine on fl cells and on healing of experimental superficial aseptic wounds in piglets
Skin Pharmacology and Physiology, 2004Co-Authors: Axel Kramer, B Roth, Gerald Muller, P Rudolph, N KlockerAbstract:The main target of the combination of Octenidine with phenoxyethanol (Octenisept®) is the antisepsis of acute wounds, whereas polyhexanide combined with polyethylene glycol in Ringer soluti
Manfred Kietzmann - One of the best experts on this subject based on the ideXlab platform.
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The percutaneous permeation of a combination of 0.1% Octenidine dihydrochloride and 2% 2-phenoxyethanol (octenisept^®) through skin of different species in vitro
BMC Veterinary Research, 2011Co-Authors: Jessica Stahl, Joerg Siebert, Michael Braun, Manfred KietzmannAbstract:BackgroundA water based combination of 0.1% Octenidine dihydrochloride and 2% 2 - phenoxyethanol is registered in many European countries as an antiseptic solution (octenisept^®) for topical treatment with high antimicrobial activity for human use, but Octenidine based products have not been registered for veterinary use yet. The aim of the present study was to investigate whether Octenidine dihydrochloride or 2 -phenoxyethanol, the two main components of this disinfectant, permeate through animal skin in vitro . Therefore, permeation studies were conducted using Franz-type diffusion cells. 2 ml of the test compound were applied onto 1.77 cm^2 split skin of cats, dogs, cows and horses. To simulate wounded skin, cattle skin was treated with adhesive tapes 100 times, as well. Up to an incubation time of 28 hours samples of the acceptor chamber were taken and were analysed by UV-HPLC. Using the method of the external standard, the apparent permeability coefficient, the flux J_max, and the recovery were calculated. Furthermore, the residues of both components in the skin samples were determined after completion of the diffusion experiment.ResultsAfter 28 hours no Octenidine dihydrochloride was found in the receptor chamber of intact skin samples, while 2.7% of the topical applied Octenidine dihydrochloride permeated through barrier disrupted cattle skin. 2 - phenoxyethanol permeated through all skin samples with the highest permeability in equine, followed by bovine, canine to feline skin. Furthermore, both components were found in the stratum corneum and the dermis of all split skin samples with different amounts in the examined species.ConclusionFor 2-phenoxyethanol the systemic impact of the high absorption rate and a potential toxicological risk have to be investigated in further studies. Due to its low absorption rates through the skin, Octenidine dihydrochloride is suitable for superficial skin treatment in the examined species.
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the percutaneous permeation of a combination of 0 1 Octenidine dihydrochloride and 2 2 phenoxyethanol octenisept through skin of different species in vitro
BMC Veterinary Research, 2011Co-Authors: Jessica Stahl, Joerg Siebert, Michael C. Braun, Manfred KietzmannAbstract:Background A water based combination of 0.1% Octenidine dihydrochloride and 2% 2 - phenoxyethanol is registered in many European countries as an antiseptic solution (octenisept®) for topical treatment with high antimicrobial activity for human use, but Octenidine based products have not been registered for veterinary use yet. The aim of the present study was to investigate whether Octenidine dihydrochloride or 2 -phenoxyethanol, the two main components of this disinfectant, permeate through animal skin in vitro. Therefore, permeation studies were conducted using Franz-type diffusion cells. 2 ml of the test compound were applied onto 1.77 cm2 split skin of cats, dogs, cows and horses. To simulate wounded skin, cattle skin was treated with adhesive tapes 100 times, as well. Up to an incubation time of 28 hours samples of the acceptor chamber were taken and were analysed by UV-HPLC. Using the method of the external standard, the apparent permeability coefficient, the flux Jmax, and the recovery were calculated. Furthermore, the residues of both components in the skin samples were determined after completion of the diffusion experiment.
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the percutaneous permeation of a combination of 0 1 Octenidine dihydrochloride and 2 2 phenoxyethanol octenisept through skin of different species in vitro
BMC Veterinary Research, 2011Co-Authors: Jessica Stahl, Joerg Siebert, Michael C. Braun, Manfred KietzmannAbstract:A water based combination of 0.1% Octenidine dihydrochloride and 2% 2 - phenoxyethanol is registered in many European countries as an antiseptic solution (octenisept®) for topical treatment with high antimicrobial activity for human use, but Octenidine based products have not been registered for veterinary use yet. The aim of the present study was to investigate whether Octenidine dihydrochloride or 2 -phenoxyethanol, the two main components of this disinfectant, permeate through animal skin in vitro. Therefore, permeation studies were conducted using Franz-type diffusion cells. 2 ml of the test compound were applied onto 1.77 cm2 split skin of cats, dogs, cows and horses. To simulate wounded skin, cattle skin was treated with adhesive tapes 100 times, as well. Up to an incubation time of 28 hours samples of the acceptor chamber were taken and were analysed by UV-HPLC. Using the method of the external standard, the apparent permeability coefficient, the flux Jmax, and the recovery were calculated. Furthermore, the residues of both components in the skin samples were determined after completion of the diffusion experiment. After 28 hours no Octenidine dihydrochloride was found in the receptor chamber of intact skin samples, while 2.7% of the topical applied Octenidine dihydrochloride permeated through barrier disrupted cattle skin. 2 - phenoxyethanol permeated through all skin samples with the highest permeability in equine, followed by bovine, canine to feline skin. Furthermore, both components were found in the stratum corneum and the dermis of all split skin samples with different amounts in the examined species. For 2-phenoxyethanol the systemic impact of the high absorption rate and a potential toxicological risk have to be investigated in further studies. Due to its low absorption rates through the skin, Octenidine dihydrochloride is suitable for superficial skin treatment in the examined species.
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the effect of a combination of 0 1 Octenidine dihydrochloride and 2 2 phenoxyethanol octenisept on wound healing in pigs in vivo and its in vitro percutaneous permeation through intact and barrier disrupted porcine skin
International Wound Journal, 2010Co-Authors: Jessica Stahl, Joerg Siebert, Michael C. Braun, Manfred KietzmannAbstract:A combination of 0.1% Octenidine dihydrochloride and 2% 2-phenoxyethanol (octenisept®) is a commonly used disinfectant in human medicine. As porcine skin represents an adequate model for human skin, the effect of Octenidine dihydrochloride and phenoxyethanol on wound healing is studied in pigs. Furthermore, the in vitro percutaneous permeation of the test substances is studied. The impact of the test formulations on wound healing is examined (A) under non occlusive conditions and (B) in comparison to another disinfectant based on povidone-iodine under occlusive conditions, while wounds are treated daily with the test substances. The percutaneous permeation of Octenidine dihydrochloride and phenoxyethanol is studied in Franz-type diffusion cells with intact skin as well as barrier disrupted after tape stripping. Compared with povidone-iodine or vehicle treatment as well as untreated control wounds the treatment of wounds with the test formulation has no influence on the healing rate in pigs and does not induce retardation of wound healing. The in vitro diffusion experiment reveals that Octenidine dihydrochloride is only detectable in the acceptor chamber of three-barrier disrupted skin samples. Phenoxyethanol permeates through intact porcine skin in amounts of 11.3% and through barrier disrupted skin in amounts of 43.9%
Jessica Stahl - One of the best experts on this subject based on the ideXlab platform.
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The percutaneous permeation of a combination of 0.1% Octenidine dihydrochloride and 2% 2-phenoxyethanol (octenisept^®) through skin of different species in vitro
BMC Veterinary Research, 2011Co-Authors: Jessica Stahl, Joerg Siebert, Michael Braun, Manfred KietzmannAbstract:BackgroundA water based combination of 0.1% Octenidine dihydrochloride and 2% 2 - phenoxyethanol is registered in many European countries as an antiseptic solution (octenisept^®) for topical treatment with high antimicrobial activity for human use, but Octenidine based products have not been registered for veterinary use yet. The aim of the present study was to investigate whether Octenidine dihydrochloride or 2 -phenoxyethanol, the two main components of this disinfectant, permeate through animal skin in vitro . Therefore, permeation studies were conducted using Franz-type diffusion cells. 2 ml of the test compound were applied onto 1.77 cm^2 split skin of cats, dogs, cows and horses. To simulate wounded skin, cattle skin was treated with adhesive tapes 100 times, as well. Up to an incubation time of 28 hours samples of the acceptor chamber were taken and were analysed by UV-HPLC. Using the method of the external standard, the apparent permeability coefficient, the flux J_max, and the recovery were calculated. Furthermore, the residues of both components in the skin samples were determined after completion of the diffusion experiment.ResultsAfter 28 hours no Octenidine dihydrochloride was found in the receptor chamber of intact skin samples, while 2.7% of the topical applied Octenidine dihydrochloride permeated through barrier disrupted cattle skin. 2 - phenoxyethanol permeated through all skin samples with the highest permeability in equine, followed by bovine, canine to feline skin. Furthermore, both components were found in the stratum corneum and the dermis of all split skin samples with different amounts in the examined species.ConclusionFor 2-phenoxyethanol the systemic impact of the high absorption rate and a potential toxicological risk have to be investigated in further studies. Due to its low absorption rates through the skin, Octenidine dihydrochloride is suitable for superficial skin treatment in the examined species.
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the percutaneous permeation of a combination of 0 1 Octenidine dihydrochloride and 2 2 phenoxyethanol octenisept through skin of different species in vitro
BMC Veterinary Research, 2011Co-Authors: Jessica Stahl, Joerg Siebert, Michael C. Braun, Manfred KietzmannAbstract:Background A water based combination of 0.1% Octenidine dihydrochloride and 2% 2 - phenoxyethanol is registered in many European countries as an antiseptic solution (octenisept®) for topical treatment with high antimicrobial activity for human use, but Octenidine based products have not been registered for veterinary use yet. The aim of the present study was to investigate whether Octenidine dihydrochloride or 2 -phenoxyethanol, the two main components of this disinfectant, permeate through animal skin in vitro. Therefore, permeation studies were conducted using Franz-type diffusion cells. 2 ml of the test compound were applied onto 1.77 cm2 split skin of cats, dogs, cows and horses. To simulate wounded skin, cattle skin was treated with adhesive tapes 100 times, as well. Up to an incubation time of 28 hours samples of the acceptor chamber were taken and were analysed by UV-HPLC. Using the method of the external standard, the apparent permeability coefficient, the flux Jmax, and the recovery were calculated. Furthermore, the residues of both components in the skin samples were determined after completion of the diffusion experiment.
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the percutaneous permeation of a combination of 0 1 Octenidine dihydrochloride and 2 2 phenoxyethanol octenisept through skin of different species in vitro
BMC Veterinary Research, 2011Co-Authors: Jessica Stahl, Joerg Siebert, Michael C. Braun, Manfred KietzmannAbstract:A water based combination of 0.1% Octenidine dihydrochloride and 2% 2 - phenoxyethanol is registered in many European countries as an antiseptic solution (octenisept®) for topical treatment with high antimicrobial activity for human use, but Octenidine based products have not been registered for veterinary use yet. The aim of the present study was to investigate whether Octenidine dihydrochloride or 2 -phenoxyethanol, the two main components of this disinfectant, permeate through animal skin in vitro. Therefore, permeation studies were conducted using Franz-type diffusion cells. 2 ml of the test compound were applied onto 1.77 cm2 split skin of cats, dogs, cows and horses. To simulate wounded skin, cattle skin was treated with adhesive tapes 100 times, as well. Up to an incubation time of 28 hours samples of the acceptor chamber were taken and were analysed by UV-HPLC. Using the method of the external standard, the apparent permeability coefficient, the flux Jmax, and the recovery were calculated. Furthermore, the residues of both components in the skin samples were determined after completion of the diffusion experiment. After 28 hours no Octenidine dihydrochloride was found in the receptor chamber of intact skin samples, while 2.7% of the topical applied Octenidine dihydrochloride permeated through barrier disrupted cattle skin. 2 - phenoxyethanol permeated through all skin samples with the highest permeability in equine, followed by bovine, canine to feline skin. Furthermore, both components were found in the stratum corneum and the dermis of all split skin samples with different amounts in the examined species. For 2-phenoxyethanol the systemic impact of the high absorption rate and a potential toxicological risk have to be investigated in further studies. Due to its low absorption rates through the skin, Octenidine dihydrochloride is suitable for superficial skin treatment in the examined species.
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the effect of a combination of 0 1 Octenidine dihydrochloride and 2 2 phenoxyethanol octenisept on wound healing in pigs in vivo and its in vitro percutaneous permeation through intact and barrier disrupted porcine skin
International Wound Journal, 2010Co-Authors: Jessica Stahl, Joerg Siebert, Michael C. Braun, Manfred KietzmannAbstract:A combination of 0.1% Octenidine dihydrochloride and 2% 2-phenoxyethanol (octenisept®) is a commonly used disinfectant in human medicine. As porcine skin represents an adequate model for human skin, the effect of Octenidine dihydrochloride and phenoxyethanol on wound healing is studied in pigs. Furthermore, the in vitro percutaneous permeation of the test substances is studied. The impact of the test formulations on wound healing is examined (A) under non occlusive conditions and (B) in comparison to another disinfectant based on povidone-iodine under occlusive conditions, while wounds are treated daily with the test substances. The percutaneous permeation of Octenidine dihydrochloride and phenoxyethanol is studied in Franz-type diffusion cells with intact skin as well as barrier disrupted after tape stripping. Compared with povidone-iodine or vehicle treatment as well as untreated control wounds the treatment of wounds with the test formulation has no influence on the healing rate in pigs and does not induce retardation of wound healing. The in vitro diffusion experiment reveals that Octenidine dihydrochloride is only detectable in the acceptor chamber of three-barrier disrupted skin samples. Phenoxyethanol permeates through intact porcine skin in amounts of 11.3% and through barrier disrupted skin in amounts of 43.9%
M Dettenkofer - One of the best experts on this subject based on the ideXlab platform.
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skin disinfection with Octenidine dihydrochloride for central venous catheter site care a double blind randomized controlled trial
Clinical Microbiology and Infection, 2010Co-Authors: M Dettenkofer, C Wilson, Alois Gratwohl, Claudia Schmoor, Hartmut Bertz, Reno Frei, Dominik Heim, D Luft, S Schulz, Andreas F WidmerAbstract:Abstract To compare the efficacy of two commercially available, alcohol-based antiseptic solutions for preparation and care of central venous catheter (CVC) insertion sites, with and without Octenidine dihydrochloride, a double-blind, randomized, controlled trial was undertaken in the haematology units and in one surgical unit of two university hospitals. Adult patients with a non-tunnelled CVC were randomly assigned to two different skin disinfection regimens at the insertion site: 0.1% Octenidine with 30% 1-propanol and 45% 2-propanol, and as control 74% ethanol with 10% 2-propanol. Endpoints were (i) skin colonization at the insertion site; (ii) -positive culture from the catheter tip (≥15 CFU); and (iii) occurrence of CVC-associated bloodstream infection (defined according to criteria set by the CDC). Four hundred patients with inserted CVC were enrolled from May 2002 through April 2005. Both groups were similar in respect of patient characteristics and co-morbidities. Skin colonization at the CVC insertion site during the first 10 days was significantly reduced by Octenidine treatment (relative difference Octenidine vs. control: 0.21; 95%CI: 0.11–0.39, p
