Oleaginous Microorganism

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Narcisa M. Bandarra - One of the best experts on this subject based on the ideXlab platform.

  • PRODUCTION OF DOCOSAHEXAENOIC ACID (DHA) FROM Thraustochytrium sp. ATCC 26185 USING DIFFERENTS NITROGEN CONCENTRATIONS
    Boletim do Centro de Pesquisa de Processamento de Alimentos, 2017
    Co-Authors: Valcenir Júnior Mendes Furlan, Júlia Ferreira, Irineu Batista, Maria Do Castelo Paulo, Victor Wegner Maus, Narcisa M. Bandarra
    Abstract:

    Polyunsaturated fatty acids (PUFAs) of the type ω3 and ω6 play important physiological functions in human organism, since they are components of cell membranes and brain cells; they decrease the levels of triglycerides and can prevent the incidence of coronary heart disease. Various parameters, including concentration of the nitrogen source in the cultivation of Oleaginous Microorganisms have been reported to be essential in the biosynthesis and accumulation of PUFAs. The objective of this work is to study the effect of different concentrations of total nitrogen (TN) in the production of PUFAs, especially DHA, from Thraustochytrium sp. ATCC 26185. The concentrations of TN evaluated were 2.4 and 0.8 g/L (batch) and 0.009 g/L (hourly) under fed-batch process. The content of cell biomass, glucose consumption, TN and production of PUFAs was determined. The major composition of the PUFAs in Thraustochytrium sp. ATCC 26185 cell biomass were DPA ω6 (21-25%) and DHA (69-73%), regardless of the type and time of culture. The maximum cell concentration obtained (30.2 g/L) was using 2.4 g/L TN in 168 h of culture. With this same concentration of TN it was possible to produce the highest concentration of DHA (1.16 g/L) in 120 h of culture, demonstrating that the growth of Thraustochytrium sp. ATCC 26185 and yield of PUFAs are dependent on the concentration TN source available for consumption of this Oleaginous Microorganism, as well as culture tim

  • PRODUCTION OF DOCOSAHEXAENOIC ACID (DHA) FROM Thraustochytrium sp. ATCC 26185 USING DIFFERENTS NITROGEN CONCENTRATIONS
    Boletim do Centro de Pesquisa de Processamento de Alimentos, 2014
    Co-Authors: Valcenir Júnior Mendes Furlan, Maria C. Paulo, Victor Maus, Júlia Ferreira, Irineu Batista, Narcisa M. Bandarra
    Abstract:

    In this work it was studied the polyunsaturated fatty acids (PUFAs) production, especially DHA, from Thraustochytrium sp. ATCC 26185, under different total nitrogen (TN) availability. Three different TN conditions were evaluated: two with initial concentrations of 2.4 g/L and 0.8 g/L, and the third in a fed-batch process with a rate of 0.009 g/L.h. For each experiment the biomass, glucose, TN and PUFAs were determined. The major composition of the PUFAs in Thraustochytrium sp. ATCC 26185 cell biomass were DPA ω6 (21-25 %) and DHA (69-73 %), regardless of the type and time of culture. The maximum cell concentration (30.2 g/L) was obtained using 2.4 g/L TN in 168 h of culture. With this same concentration of TN it was possible to produce the highest concentration of DHA (1.16 g/L) in 120 h of culture, demonstrating that the growth of Thraustochytrium sp. ATCC 26185 and yield of PUFAs are dependent on the source concentration of TN available for consumption of this Oleaginous Microorganism, as well as culture time.

Yeekchia Ho - One of the best experts on this subject based on the ideXlab platform.

  • third generation biofuels a nutritional perspective in enhancing microbial lipid production
    Renewable & Sustainable Energy Reviews, 2018
    Co-Authors: Waihong Leong, Yoshimitsu Uemura, Yeekchia Ho
    Abstract:

    Abstract The third generation biofuels derived from Oleaginous Microorganisms have gained traction recently as the potential feedstock in generating fuel for energy production, reducing the direct dependence on fossil fuels. However, commercialization of microbial technology for biofuel production remains intricate and questionable due to many factors concerning the life cycle assessment and techno-economic feasibility of Microorganisms-based biofuels. This review initially focuses on the nutritional aspects in enhancing the biomass and lipid yields from various Microorganism feedstock, serving as impetuses for the production of third generation biofuels. Nutrient optimizations in terms of nutrient starvation, supplementation and balancing technique are discussed in relation to their respective effects on the microbial biomass and lipid productions. More importantly, the economic perspectives of Oleaginous Microorganism cultivations are also reviewed and strategized using alternative and non-conventional nutrient sources for possible technology scale-ups and commercialization.

Valcenir Júnior Mendes Furlan - One of the best experts on this subject based on the ideXlab platform.

  • PRODUCTION OF DOCOSAHEXAENOIC ACID (DHA) FROM Thraustochytrium sp. ATCC 26185 USING DIFFERENTS NITROGEN CONCENTRATIONS
    Boletim do Centro de Pesquisa de Processamento de Alimentos, 2017
    Co-Authors: Valcenir Júnior Mendes Furlan, Júlia Ferreira, Irineu Batista, Maria Do Castelo Paulo, Victor Wegner Maus, Narcisa M. Bandarra
    Abstract:

    Polyunsaturated fatty acids (PUFAs) of the type ω3 and ω6 play important physiological functions in human organism, since they are components of cell membranes and brain cells; they decrease the levels of triglycerides and can prevent the incidence of coronary heart disease. Various parameters, including concentration of the nitrogen source in the cultivation of Oleaginous Microorganisms have been reported to be essential in the biosynthesis and accumulation of PUFAs. The objective of this work is to study the effect of different concentrations of total nitrogen (TN) in the production of PUFAs, especially DHA, from Thraustochytrium sp. ATCC 26185. The concentrations of TN evaluated were 2.4 and 0.8 g/L (batch) and 0.009 g/L (hourly) under fed-batch process. The content of cell biomass, glucose consumption, TN and production of PUFAs was determined. The major composition of the PUFAs in Thraustochytrium sp. ATCC 26185 cell biomass were DPA ω6 (21-25%) and DHA (69-73%), regardless of the type and time of culture. The maximum cell concentration obtained (30.2 g/L) was using 2.4 g/L TN in 168 h of culture. With this same concentration of TN it was possible to produce the highest concentration of DHA (1.16 g/L) in 120 h of culture, demonstrating that the growth of Thraustochytrium sp. ATCC 26185 and yield of PUFAs are dependent on the concentration TN source available for consumption of this Oleaginous Microorganism, as well as culture tim

  • PRODUCTION OF DOCOSAHEXAENOIC ACID (DHA) FROM Thraustochytrium sp. ATCC 26185 USING DIFFERENTS NITROGEN CONCENTRATIONS
    Boletim do Centro de Pesquisa de Processamento de Alimentos, 2014
    Co-Authors: Valcenir Júnior Mendes Furlan, Maria C. Paulo, Victor Maus, Júlia Ferreira, Irineu Batista, Narcisa M. Bandarra
    Abstract:

    In this work it was studied the polyunsaturated fatty acids (PUFAs) production, especially DHA, from Thraustochytrium sp. ATCC 26185, under different total nitrogen (TN) availability. Three different TN conditions were evaluated: two with initial concentrations of 2.4 g/L and 0.8 g/L, and the third in a fed-batch process with a rate of 0.009 g/L.h. For each experiment the biomass, glucose, TN and PUFAs were determined. The major composition of the PUFAs in Thraustochytrium sp. ATCC 26185 cell biomass were DPA ω6 (21-25 %) and DHA (69-73 %), regardless of the type and time of culture. The maximum cell concentration (30.2 g/L) was obtained using 2.4 g/L TN in 168 h of culture. With this same concentration of TN it was possible to produce the highest concentration of DHA (1.16 g/L) in 120 h of culture, demonstrating that the growth of Thraustochytrium sp. ATCC 26185 and yield of PUFAs are dependent on the source concentration of TN available for consumption of this Oleaginous Microorganism, as well as culture time.

Yongkui Zhang - One of the best experts on this subject based on the ideXlab platform.

  • Enhanced lipid production and nutrient utilization of food waste hydrolysate by mixed culture of Oleaginous yeast Rhodosporidium toruloides and Oleaginous microalgae Chlorella vulgaris
    Renewable Energy, 2018
    Co-Authors: Yu Zeng, Tonghui Xie, Banggao Jian, Yi Xie, Yongkui Zhang
    Abstract:

    Abstract Using food waste hydrolysate as medium for culturing Oleaginous Microorganism is a dual-purpose strategy for relieving environmental burdens both in food waste disposal and energy crisis. The pure culture of one Oleaginous Microorganism when single waste as culture media has shortages such as high operation cost, low lipid production and low substrate utilization. Mix-culture of yeast and microalgae in this work enhanced this process result in better waste treatment, higher lipid production and shorter cultivation time. Compare to pure culture of Rhodosporidium toruloides, the nitrogen utilization rate, reducing sugar utilization rate and lipid production increased by 23%, 17% and 12%, respectively, and the cultivation time shorten by 40%, in mix-culture of Rhodosporidium toruloides and Chlorella vulgaris. The results showed that the mix-culture of yeast and microalgae using food waste hydrolysate as culture medium would be a good strategy for both waste disposal and biofuel production.

Irineu Batista - One of the best experts on this subject based on the ideXlab platform.

  • PRODUCTION OF DOCOSAHEXAENOIC ACID (DHA) FROM Thraustochytrium sp. ATCC 26185 USING DIFFERENTS NITROGEN CONCENTRATIONS
    Boletim do Centro de Pesquisa de Processamento de Alimentos, 2017
    Co-Authors: Valcenir Júnior Mendes Furlan, Júlia Ferreira, Irineu Batista, Maria Do Castelo Paulo, Victor Wegner Maus, Narcisa M. Bandarra
    Abstract:

    Polyunsaturated fatty acids (PUFAs) of the type ω3 and ω6 play important physiological functions in human organism, since they are components of cell membranes and brain cells; they decrease the levels of triglycerides and can prevent the incidence of coronary heart disease. Various parameters, including concentration of the nitrogen source in the cultivation of Oleaginous Microorganisms have been reported to be essential in the biosynthesis and accumulation of PUFAs. The objective of this work is to study the effect of different concentrations of total nitrogen (TN) in the production of PUFAs, especially DHA, from Thraustochytrium sp. ATCC 26185. The concentrations of TN evaluated were 2.4 and 0.8 g/L (batch) and 0.009 g/L (hourly) under fed-batch process. The content of cell biomass, glucose consumption, TN and production of PUFAs was determined. The major composition of the PUFAs in Thraustochytrium sp. ATCC 26185 cell biomass were DPA ω6 (21-25%) and DHA (69-73%), regardless of the type and time of culture. The maximum cell concentration obtained (30.2 g/L) was using 2.4 g/L TN in 168 h of culture. With this same concentration of TN it was possible to produce the highest concentration of DHA (1.16 g/L) in 120 h of culture, demonstrating that the growth of Thraustochytrium sp. ATCC 26185 and yield of PUFAs are dependent on the concentration TN source available for consumption of this Oleaginous Microorganism, as well as culture tim

  • PRODUCTION OF DOCOSAHEXAENOIC ACID (DHA) FROM Thraustochytrium sp. ATCC 26185 USING DIFFERENTS NITROGEN CONCENTRATIONS
    Boletim do Centro de Pesquisa de Processamento de Alimentos, 2014
    Co-Authors: Valcenir Júnior Mendes Furlan, Maria C. Paulo, Victor Maus, Júlia Ferreira, Irineu Batista, Narcisa M. Bandarra
    Abstract:

    In this work it was studied the polyunsaturated fatty acids (PUFAs) production, especially DHA, from Thraustochytrium sp. ATCC 26185, under different total nitrogen (TN) availability. Three different TN conditions were evaluated: two with initial concentrations of 2.4 g/L and 0.8 g/L, and the third in a fed-batch process with a rate of 0.009 g/L.h. For each experiment the biomass, glucose, TN and PUFAs were determined. The major composition of the PUFAs in Thraustochytrium sp. ATCC 26185 cell biomass were DPA ω6 (21-25 %) and DHA (69-73 %), regardless of the type and time of culture. The maximum cell concentration (30.2 g/L) was obtained using 2.4 g/L TN in 168 h of culture. With this same concentration of TN it was possible to produce the highest concentration of DHA (1.16 g/L) in 120 h of culture, demonstrating that the growth of Thraustochytrium sp. ATCC 26185 and yield of PUFAs are dependent on the source concentration of TN available for consumption of this Oleaginous Microorganism, as well as culture time.