The Experts below are selected from a list of 31293 Experts worldwide ranked by ideXlab platform
Molly Dumanscheel - One of the best experts on this subject based on the ideXlab platform.
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chitosan sirna nanoparticle targeting demonstrates a requirement for single minded during larval and pupal Olfactory System development of the vector mosquito aedes aegypti
BMC Developmental Biology, 2014Co-Authors: Keshava Mysore, Emily Andrews, Ping Li, Molly DumanscheelAbstract:Background Essentially nothing is known about the genetic regulation of Olfactory System development in vector mosquitoes, which use Olfactory cues to detect blood meal hosts. Studies in Drosophila melanogaster have identified a regulatory matrix of transcription factors that controls pupal/adult odorant receptor (OR) gene expression in Olfactory receptor neurons (ORNs). However, it is unclear if transcription factors that function in the D. melanogaster regulatory matrix are required for OR expression in mosquitoes. Furthermore, the regulation of OR expression during development of the larval Olfactory System, which is far less complex than that of pupae/adults, is not well understood in any insect, including D. melanogaster. Here, we examine the regulation of OR expression in the developing larval Olfactory System of Aedes aegypti, the dengue vector mosquito.
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disruption of aedes aegypti Olfactory System development through chitosan sirna nanoparticle targeting of semaphorin 1a
PLOS Neglected Tropical Diseases, 2013Co-Authors: Keshava Mysore, Molly Dumanscheel, Ellen Flannery, Michael Tomchaney, David W SeversonAbstract:Despite the devastating impact of mosquito-borne illnesses on human health, surprisingly little is known about mosquito developmental biology, including development of the Olfactory System, a tissue of vector importance. Analysis of mosquito Olfactory developmental genetics has been hindered by a lack of means to target specific genes during the development of this sensory System. In this investigation, chitosan/siRNA nanoparticles were used to target semaphorin-1a (sema1a) during Olfactory System development in the dengue and yellow fever vector mosquito Aedes aegypti. Immunohistochemical analyses and anterograde tracing of antennal sensory neurons, which were used to track the progression of Olfactory development in this species, revealed antennal lobe defects in sema1a knockdown fourth instar larvae. These findings, which correlated with a larval odorant tracking behavioral phenotype, identified previously unreported roles for Sema1a in the developing insect larval Olfactory System. Analysis of sema1a knockdown pupae also revealed a number of Olfactory phenotypes, including Olfactory receptor neuron targeting and projection neuron defects coincident with a collapse in the structure and shape of the antennal lobe and individual glomeruli. This study, which is to our knowledge the first functional genetic analysis of insect Olfactory development outside of D. melanogaster, identified critical roles for Sema1a during Ae. aegypti larval and pupal Olfactory development and advocates the use of chitosan/siRNA nanoparticles as an effective means of targeting genes during post-embryonic Ae. aegypti development. Use of siRNA nanoparticle methodology to understand sensory developmental genetics in mosquitoes will provide insight into the evolutionary conservation and divergence of key developmental genes which could be exploited in the development of both common and species-specific means for intervention.
Keshava Mysore - One of the best experts on this subject based on the ideXlab platform.
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chitosan sirna nanoparticle targeting demonstrates a requirement for single minded during larval and pupal Olfactory System development of the vector mosquito aedes aegypti
BMC Developmental Biology, 2014Co-Authors: Keshava Mysore, Emily Andrews, Ping Li, Molly DumanscheelAbstract:Background Essentially nothing is known about the genetic regulation of Olfactory System development in vector mosquitoes, which use Olfactory cues to detect blood meal hosts. Studies in Drosophila melanogaster have identified a regulatory matrix of transcription factors that controls pupal/adult odorant receptor (OR) gene expression in Olfactory receptor neurons (ORNs). However, it is unclear if transcription factors that function in the D. melanogaster regulatory matrix are required for OR expression in mosquitoes. Furthermore, the regulation of OR expression during development of the larval Olfactory System, which is far less complex than that of pupae/adults, is not well understood in any insect, including D. melanogaster. Here, we examine the regulation of OR expression in the developing larval Olfactory System of Aedes aegypti, the dengue vector mosquito.
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disruption of aedes aegypti Olfactory System development through chitosan sirna nanoparticle targeting of semaphorin 1a
PLOS Neglected Tropical Diseases, 2013Co-Authors: Keshava Mysore, Molly Dumanscheel, Ellen Flannery, Michael Tomchaney, David W SeversonAbstract:Despite the devastating impact of mosquito-borne illnesses on human health, surprisingly little is known about mosquito developmental biology, including development of the Olfactory System, a tissue of vector importance. Analysis of mosquito Olfactory developmental genetics has been hindered by a lack of means to target specific genes during the development of this sensory System. In this investigation, chitosan/siRNA nanoparticles were used to target semaphorin-1a (sema1a) during Olfactory System development in the dengue and yellow fever vector mosquito Aedes aegypti. Immunohistochemical analyses and anterograde tracing of antennal sensory neurons, which were used to track the progression of Olfactory development in this species, revealed antennal lobe defects in sema1a knockdown fourth instar larvae. These findings, which correlated with a larval odorant tracking behavioral phenotype, identified previously unreported roles for Sema1a in the developing insect larval Olfactory System. Analysis of sema1a knockdown pupae also revealed a number of Olfactory phenotypes, including Olfactory receptor neuron targeting and projection neuron defects coincident with a collapse in the structure and shape of the antennal lobe and individual glomeruli. This study, which is to our knowledge the first functional genetic analysis of insect Olfactory development outside of D. melanogaster, identified critical roles for Sema1a during Ae. aegypti larval and pupal Olfactory development and advocates the use of chitosan/siRNA nanoparticles as an effective means of targeting genes during post-embryonic Ae. aegypti development. Use of siRNA nanoparticle methodology to understand sensory developmental genetics in mosquitoes will provide insight into the evolutionary conservation and divergence of key developmental genes which could be exploited in the development of both common and species-specific means for intervention.
Roger Croll - One of the best experts on this subject based on the ideXlab platform.
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Histamine and histidine decarboxylase in the Olfactory System and brain of the common cuttlefish Sepia officinalis (Linnaeus, 1758)
Journal of Comparative Neurology, 2019Co-Authors: Alexia Scaros, Aude Andouche, Sébastien Baratte, Roger CrollAbstract:Cephalopods are radically different from any other invertebrate. Their molluscan heritage, innovative nervous System, and specialized behaviors create a unique blend of characteristics that are sometimes reminiscent of vertebrate features. For example, despite differences in the organization and development of their nervous Systems, both vertebrates and cephalopods use many of the same neurotransmitters. One neurotransmitter, histamine (HA), has been well studied in both vertebrates and invertebrates, including molluscs. While HA was previously suggested to be present in the cephalopod central nervous System (CNS), Scaros, Croll, and Baratte only recently described the localization of HA in the Olfactory System of the cuttlefish Sepia officinalis. Here, we describe the location of HA using an anti‐HA antibody and a probe for histidine decarboxylase (HDC), a synthetic enzyme for HA. We extended previous descriptions of HA in the Olfactory organ, nerve, and lobe, and describe HDC staining in the same regions. We found HDC‐positive cell populations throughout the CNS, including the optic gland and the peduncle, optic, dorso‐lateral, basal, subvertical, frontal, magnocellular, and buccal lobes. The distribution of HA in the Olfactory System of S. officinalis is similar to the presence of HA in the chemosensory organs of gastropods but is different than the sensory Systems in vertebrates or arthropods. However, HA's widespread abundance throughout the rest of the CNS of Sepia is a similarity shared with gastropods, vertebrates, and arthropods. Its widespread use with differing functions across Animalia provokes questions regarding the evolutionary history and adaptability of HA as a transmitter.
Alexia Scaros - One of the best experts on this subject based on the ideXlab platform.
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Histamine and histidine decarboxylase in the Olfactory System and brain of the common cuttlefish Sepia officinalis (Linnaeus, 1758)
Journal of Comparative Neurology, 2019Co-Authors: Alexia Scaros, Aude Andouche, Sébastien Baratte, Roger CrollAbstract:Cephalopods are radically different from any other invertebrate. Their molluscan heritage, innovative nervous System, and specialized behaviors create a unique blend of characteristics that are sometimes reminiscent of vertebrate features. For example, despite differences in the organization and development of their nervous Systems, both vertebrates and cephalopods use many of the same neurotransmitters. One neurotransmitter, histamine (HA), has been well studied in both vertebrates and invertebrates, including molluscs. While HA was previously suggested to be present in the cephalopod central nervous System (CNS), Scaros, Croll, and Baratte only recently described the localization of HA in the Olfactory System of the cuttlefish Sepia officinalis. Here, we describe the location of HA using an anti‐HA antibody and a probe for histidine decarboxylase (HDC), a synthetic enzyme for HA. We extended previous descriptions of HA in the Olfactory organ, nerve, and lobe, and describe HDC staining in the same regions. We found HDC‐positive cell populations throughout the CNS, including the optic gland and the peduncle, optic, dorso‐lateral, basal, subvertical, frontal, magnocellular, and buccal lobes. The distribution of HA in the Olfactory System of S. officinalis is similar to the presence of HA in the chemosensory organs of gastropods but is different than the sensory Systems in vertebrates or arthropods. However, HA's widespread abundance throughout the rest of the CNS of Sepia is a similarity shared with gastropods, vertebrates, and arthropods. Its widespread use with differing functions across Animalia provokes questions regarding the evolutionary history and adaptability of HA as a transmitter.
David W Severson - One of the best experts on this subject based on the ideXlab platform.
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disruption of aedes aegypti Olfactory System development through chitosan sirna nanoparticle targeting of semaphorin 1a
PLOS Neglected Tropical Diseases, 2013Co-Authors: Keshava Mysore, Molly Dumanscheel, Ellen Flannery, Michael Tomchaney, David W SeversonAbstract:Despite the devastating impact of mosquito-borne illnesses on human health, surprisingly little is known about mosquito developmental biology, including development of the Olfactory System, a tissue of vector importance. Analysis of mosquito Olfactory developmental genetics has been hindered by a lack of means to target specific genes during the development of this sensory System. In this investigation, chitosan/siRNA nanoparticles were used to target semaphorin-1a (sema1a) during Olfactory System development in the dengue and yellow fever vector mosquito Aedes aegypti. Immunohistochemical analyses and anterograde tracing of antennal sensory neurons, which were used to track the progression of Olfactory development in this species, revealed antennal lobe defects in sema1a knockdown fourth instar larvae. These findings, which correlated with a larval odorant tracking behavioral phenotype, identified previously unreported roles for Sema1a in the developing insect larval Olfactory System. Analysis of sema1a knockdown pupae also revealed a number of Olfactory phenotypes, including Olfactory receptor neuron targeting and projection neuron defects coincident with a collapse in the structure and shape of the antennal lobe and individual glomeruli. This study, which is to our knowledge the first functional genetic analysis of insect Olfactory development outside of D. melanogaster, identified critical roles for Sema1a during Ae. aegypti larval and pupal Olfactory development and advocates the use of chitosan/siRNA nanoparticles as an effective means of targeting genes during post-embryonic Ae. aegypti development. Use of siRNA nanoparticle methodology to understand sensory developmental genetics in mosquitoes will provide insight into the evolutionary conservation and divergence of key developmental genes which could be exploited in the development of both common and species-specific means for intervention.
