The Experts below are selected from a list of 3282 Experts worldwide ranked by ideXlab platform
Geeta Shukla - One of the best experts on this subject based on the ideXlab platform.
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prophylactic intervention of probiotics l acidophilus l rhamnosus gg and celecoxib modulate bax mediated apoptosis in 1 2 dimethylhydrazine induced experimental colon carcinogenesis
BMC Cancer, 2018Co-Authors: Leila Kaeid Sharaf, Mridul Sharma, Deepika Chandel, Geeta ShuklaAbstract:Colorectal cancer has been found to be attenuated either with prophylactic manipulation of gut microbiome with probiotics or celecoxib, a non-steroidal anti-inflammatory drug mainly by suppressing early pro-carcinogenic marKers in various experimental studies. Therefore, the present study was designed to assess the prophylactic potential of combinatorial administration of probiotics (Lactobacillus rhamnosus GG, Lactobacillus acidophilus) and celecoxib in experimental colon carcinogenesis. Six groups of Spraugue Dawely rats received probiotics L.rhamnosus GG or/and L.acidophilus in combination with celecoxib one weeK prior to the inducement of tumor by 1,2-dimethylhydrazine (DMH) and the treatment continued for 18 weeKs. Prophylactic potentials of probiotics and celecoxib were determined by employing various methods such as tumor incidence, tumor burden, tumor multiplicity, apoptosis, caspase activity, expression of proto-Oncogene K-Ras and tumor suppressor p53 gene in colonic tumors. Interestingly, it was found that one weeK prior supplementation of both probiotics and celecoxib reduced tumor burden, tumor multiplicity, down-regulated the expression of anti-apoptotic Bcl-2, proto-Oncogene K-Ras and up-regulated pro-apoptotic Bax as well as tumor suppressor p53 in L.rhamnosus GG + celecoxib+DMH animals compared with counter controls and DMH-treated. It can be concluded that such combinatorial approach may be useful in reducing the burden and severity of disease in highly susceptible individuals but needs to be validated clinically.
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Prophylactic intervention of probiotics (L.acidophilus, L.rhamnosus GG) and celecoxib modulate Bax-mediated apoptosis in 1,2-dimethylhydrazine-induced experimental colon carcinogenesis
BMC, 2018Co-Authors: Leila Kaeid Sharaf, Mridul Sharma, Deepika Chandel, Geeta ShuklaAbstract:Abstract BacKground Colorectal cancer has been found to be attenuated either with prophylactic manipulation of gut microbiome with probiotics or celecoxib, a non-steroidal anti-inflammatory drug mainly by suppressing early pro-carcinogenic marKers in various experimental studies. Therefore, the present study was designed to assess the prophylactic potential of combinatorial administration of probiotics (Lactobacillus rhamnosus GG, Lactobacillus acidophilus) and celecoxib in experimental colon carcinogenesis. Methods Six groups of Spraugue Dawely rats received probiotics L.rhamnosus GG or/and L.acidophilus in combination with celecoxib one weeK prior to the inducement of tumor by 1,2-dimethylhydrazine (DMH) and the treatment continued for 18 weeKs. Prophylactic potentials of probiotics and celecoxib were determined by employing various methods such as tumor incidence, tumor burden, tumor multiplicity, apoptosis, caspase activity, expression of proto-Oncogene K-Ras and tumor suppressor p53 gene in colonic tumors. Results Interestingly, it was found that one weeK prior supplementation of both probiotics and celecoxib reduced tumor burden, tumor multiplicity, down-regulated the expression of anti-apoptotic Bcl-2, proto-Oncogene K-Ras and up-regulated pro-apoptotic Bax as well as tumor suppressor p53 in L.rhamnosus GG + celecoxib+DMH animals compared with counter controls and DMH-treated. Conclusions It can be concluded that such combinatorial approach may be useful in reducing the burden and severity of disease in highly susceptible individuals but needs to be validated clinically
Leila Kaeid Sharaf - One of the best experts on this subject based on the ideXlab platform.
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prophylactic intervention of probiotics l acidophilus l rhamnosus gg and celecoxib modulate bax mediated apoptosis in 1 2 dimethylhydrazine induced experimental colon carcinogenesis
BMC Cancer, 2018Co-Authors: Leila Kaeid Sharaf, Mridul Sharma, Deepika Chandel, Geeta ShuklaAbstract:Colorectal cancer has been found to be attenuated either with prophylactic manipulation of gut microbiome with probiotics or celecoxib, a non-steroidal anti-inflammatory drug mainly by suppressing early pro-carcinogenic marKers in various experimental studies. Therefore, the present study was designed to assess the prophylactic potential of combinatorial administration of probiotics (Lactobacillus rhamnosus GG, Lactobacillus acidophilus) and celecoxib in experimental colon carcinogenesis. Six groups of Spraugue Dawely rats received probiotics L.rhamnosus GG or/and L.acidophilus in combination with celecoxib one weeK prior to the inducement of tumor by 1,2-dimethylhydrazine (DMH) and the treatment continued for 18 weeKs. Prophylactic potentials of probiotics and celecoxib were determined by employing various methods such as tumor incidence, tumor burden, tumor multiplicity, apoptosis, caspase activity, expression of proto-Oncogene K-Ras and tumor suppressor p53 gene in colonic tumors. Interestingly, it was found that one weeK prior supplementation of both probiotics and celecoxib reduced tumor burden, tumor multiplicity, down-regulated the expression of anti-apoptotic Bcl-2, proto-Oncogene K-Ras and up-regulated pro-apoptotic Bax as well as tumor suppressor p53 in L.rhamnosus GG + celecoxib+DMH animals compared with counter controls and DMH-treated. It can be concluded that such combinatorial approach may be useful in reducing the burden and severity of disease in highly susceptible individuals but needs to be validated clinically.
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Prophylactic intervention of probiotics (L.acidophilus, L.rhamnosus GG) and celecoxib modulate Bax-mediated apoptosis in 1,2-dimethylhydrazine-induced experimental colon carcinogenesis
BMC, 2018Co-Authors: Leila Kaeid Sharaf, Mridul Sharma, Deepika Chandel, Geeta ShuklaAbstract:Abstract BacKground Colorectal cancer has been found to be attenuated either with prophylactic manipulation of gut microbiome with probiotics or celecoxib, a non-steroidal anti-inflammatory drug mainly by suppressing early pro-carcinogenic marKers in various experimental studies. Therefore, the present study was designed to assess the prophylactic potential of combinatorial administration of probiotics (Lactobacillus rhamnosus GG, Lactobacillus acidophilus) and celecoxib in experimental colon carcinogenesis. Methods Six groups of Spraugue Dawely rats received probiotics L.rhamnosus GG or/and L.acidophilus in combination with celecoxib one weeK prior to the inducement of tumor by 1,2-dimethylhydrazine (DMH) and the treatment continued for 18 weeKs. Prophylactic potentials of probiotics and celecoxib were determined by employing various methods such as tumor incidence, tumor burden, tumor multiplicity, apoptosis, caspase activity, expression of proto-Oncogene K-Ras and tumor suppressor p53 gene in colonic tumors. Results Interestingly, it was found that one weeK prior supplementation of both probiotics and celecoxib reduced tumor burden, tumor multiplicity, down-regulated the expression of anti-apoptotic Bcl-2, proto-Oncogene K-Ras and up-regulated pro-apoptotic Bax as well as tumor suppressor p53 in L.rhamnosus GG + celecoxib+DMH animals compared with counter controls and DMH-treated. Conclusions It can be concluded that such combinatorial approach may be useful in reducing the burden and severity of disease in highly susceptible individuals but needs to be validated clinically
Deepika Chandel - One of the best experts on this subject based on the ideXlab platform.
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prophylactic intervention of probiotics l acidophilus l rhamnosus gg and celecoxib modulate bax mediated apoptosis in 1 2 dimethylhydrazine induced experimental colon carcinogenesis
BMC Cancer, 2018Co-Authors: Leila Kaeid Sharaf, Mridul Sharma, Deepika Chandel, Geeta ShuklaAbstract:Colorectal cancer has been found to be attenuated either with prophylactic manipulation of gut microbiome with probiotics or celecoxib, a non-steroidal anti-inflammatory drug mainly by suppressing early pro-carcinogenic marKers in various experimental studies. Therefore, the present study was designed to assess the prophylactic potential of combinatorial administration of probiotics (Lactobacillus rhamnosus GG, Lactobacillus acidophilus) and celecoxib in experimental colon carcinogenesis. Six groups of Spraugue Dawely rats received probiotics L.rhamnosus GG or/and L.acidophilus in combination with celecoxib one weeK prior to the inducement of tumor by 1,2-dimethylhydrazine (DMH) and the treatment continued for 18 weeKs. Prophylactic potentials of probiotics and celecoxib were determined by employing various methods such as tumor incidence, tumor burden, tumor multiplicity, apoptosis, caspase activity, expression of proto-Oncogene K-Ras and tumor suppressor p53 gene in colonic tumors. Interestingly, it was found that one weeK prior supplementation of both probiotics and celecoxib reduced tumor burden, tumor multiplicity, down-regulated the expression of anti-apoptotic Bcl-2, proto-Oncogene K-Ras and up-regulated pro-apoptotic Bax as well as tumor suppressor p53 in L.rhamnosus GG + celecoxib+DMH animals compared with counter controls and DMH-treated. It can be concluded that such combinatorial approach may be useful in reducing the burden and severity of disease in highly susceptible individuals but needs to be validated clinically.
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Prophylactic intervention of probiotics (L.acidophilus, L.rhamnosus GG) and celecoxib modulate Bax-mediated apoptosis in 1,2-dimethylhydrazine-induced experimental colon carcinogenesis
BMC, 2018Co-Authors: Leila Kaeid Sharaf, Mridul Sharma, Deepika Chandel, Geeta ShuklaAbstract:Abstract BacKground Colorectal cancer has been found to be attenuated either with prophylactic manipulation of gut microbiome with probiotics or celecoxib, a non-steroidal anti-inflammatory drug mainly by suppressing early pro-carcinogenic marKers in various experimental studies. Therefore, the present study was designed to assess the prophylactic potential of combinatorial administration of probiotics (Lactobacillus rhamnosus GG, Lactobacillus acidophilus) and celecoxib in experimental colon carcinogenesis. Methods Six groups of Spraugue Dawely rats received probiotics L.rhamnosus GG or/and L.acidophilus in combination with celecoxib one weeK prior to the inducement of tumor by 1,2-dimethylhydrazine (DMH) and the treatment continued for 18 weeKs. Prophylactic potentials of probiotics and celecoxib were determined by employing various methods such as tumor incidence, tumor burden, tumor multiplicity, apoptosis, caspase activity, expression of proto-Oncogene K-Ras and tumor suppressor p53 gene in colonic tumors. Results Interestingly, it was found that one weeK prior supplementation of both probiotics and celecoxib reduced tumor burden, tumor multiplicity, down-regulated the expression of anti-apoptotic Bcl-2, proto-Oncogene K-Ras and up-regulated pro-apoptotic Bax as well as tumor suppressor p53 in L.rhamnosus GG + celecoxib+DMH animals compared with counter controls and DMH-treated. Conclusions It can be concluded that such combinatorial approach may be useful in reducing the burden and severity of disease in highly susceptible individuals but needs to be validated clinically
Mridul Sharma - One of the best experts on this subject based on the ideXlab platform.
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prophylactic intervention of probiotics l acidophilus l rhamnosus gg and celecoxib modulate bax mediated apoptosis in 1 2 dimethylhydrazine induced experimental colon carcinogenesis
BMC Cancer, 2018Co-Authors: Leila Kaeid Sharaf, Mridul Sharma, Deepika Chandel, Geeta ShuklaAbstract:Colorectal cancer has been found to be attenuated either with prophylactic manipulation of gut microbiome with probiotics or celecoxib, a non-steroidal anti-inflammatory drug mainly by suppressing early pro-carcinogenic marKers in various experimental studies. Therefore, the present study was designed to assess the prophylactic potential of combinatorial administration of probiotics (Lactobacillus rhamnosus GG, Lactobacillus acidophilus) and celecoxib in experimental colon carcinogenesis. Six groups of Spraugue Dawely rats received probiotics L.rhamnosus GG or/and L.acidophilus in combination with celecoxib one weeK prior to the inducement of tumor by 1,2-dimethylhydrazine (DMH) and the treatment continued for 18 weeKs. Prophylactic potentials of probiotics and celecoxib were determined by employing various methods such as tumor incidence, tumor burden, tumor multiplicity, apoptosis, caspase activity, expression of proto-Oncogene K-Ras and tumor suppressor p53 gene in colonic tumors. Interestingly, it was found that one weeK prior supplementation of both probiotics and celecoxib reduced tumor burden, tumor multiplicity, down-regulated the expression of anti-apoptotic Bcl-2, proto-Oncogene K-Ras and up-regulated pro-apoptotic Bax as well as tumor suppressor p53 in L.rhamnosus GG + celecoxib+DMH animals compared with counter controls and DMH-treated. It can be concluded that such combinatorial approach may be useful in reducing the burden and severity of disease in highly susceptible individuals but needs to be validated clinically.
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Prophylactic intervention of probiotics (L.acidophilus, L.rhamnosus GG) and celecoxib modulate Bax-mediated apoptosis in 1,2-dimethylhydrazine-induced experimental colon carcinogenesis
BMC, 2018Co-Authors: Leila Kaeid Sharaf, Mridul Sharma, Deepika Chandel, Geeta ShuklaAbstract:Abstract BacKground Colorectal cancer has been found to be attenuated either with prophylactic manipulation of gut microbiome with probiotics or celecoxib, a non-steroidal anti-inflammatory drug mainly by suppressing early pro-carcinogenic marKers in various experimental studies. Therefore, the present study was designed to assess the prophylactic potential of combinatorial administration of probiotics (Lactobacillus rhamnosus GG, Lactobacillus acidophilus) and celecoxib in experimental colon carcinogenesis. Methods Six groups of Spraugue Dawely rats received probiotics L.rhamnosus GG or/and L.acidophilus in combination with celecoxib one weeK prior to the inducement of tumor by 1,2-dimethylhydrazine (DMH) and the treatment continued for 18 weeKs. Prophylactic potentials of probiotics and celecoxib were determined by employing various methods such as tumor incidence, tumor burden, tumor multiplicity, apoptosis, caspase activity, expression of proto-Oncogene K-Ras and tumor suppressor p53 gene in colonic tumors. Results Interestingly, it was found that one weeK prior supplementation of both probiotics and celecoxib reduced tumor burden, tumor multiplicity, down-regulated the expression of anti-apoptotic Bcl-2, proto-Oncogene K-Ras and up-regulated pro-apoptotic Bax as well as tumor suppressor p53 in L.rhamnosus GG + celecoxib+DMH animals compared with counter controls and DMH-treated. Conclusions It can be concluded that such combinatorial approach may be useful in reducing the burden and severity of disease in highly susceptible individuals but needs to be validated clinically
Kazunori Nagasaka - One of the best experts on this subject based on the ideXlab platform.
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the modulation of adaptive immune response by Oncogene K Ras mouse model of ovarian cancer
Journal of Reproductive Immunology, 2015Co-Authors: Juri Takahashi, Kei Kawana, Ayumi Taguchi, Mitsuyo Yoshida, Hiroe Nakamura, Asaha Fujimoto, Masakazu Sato, Tomoko Inoue, Katsuyuki Adachi, Kazunori NagasakaAbstract:Purpose: Little is Known about how Oncogenes affect the immune response in tumor microenvironment (TME). In this study, we focused on how Oncogenes modulate adaptive immune response of ovarian cancer with peritoneal carcinomatosis and investigated the difference between reproductive immunity and tumor immunity. Methods: K-Ras was stably introduced intomurine ovarian cancer cell line: ID8. Each cell line was intra-peritoneal injected into mice, and the production of ascites was observed. The population of T cell and dendritic cell (DC) subsets of spleen and ascites was assessedbyCD8/CD4orCD11c/mPDCAdouble-stainedflowcytometry. Results: K-Ras enhanced the production of ascites. Compared to control mice, the CD8/CD4 ratio was increased in the cancer induced ascites without any difference between ID8 and ID8KRas. CD11c high mPDCA negative DC subset was increased in the ID8-induced ascites, which was marKedly decreased in the ID8KRas-induced ascites. There was no significant difference in the subset of T cell or DC of spleen. Conclusion: K-Ras accelerated cancer progression by modulating immune response in TME. In the tumor immune system, Oncogenes, being closely related to the modulation of TME, are responsible for its specific characteristics compared to the reproductive immunity.
