The Experts below are selected from a list of 19392 Experts worldwide ranked by ideXlab platform
Brian Austin - One of the best experts on this subject based on the ideXlab platform.
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cellular components of probiotics control yersinia ruckeri infection in rainbow trout Oncorhynchus mykiss walbaum
Journal of Fish Diseases, 2010Co-Authors: Amany Abbass, S M Sharifuzzaman, Brian AustinAbstract:Subcellular components of the probiotics Aeromonas sobria GC2 and Bacillus subtilis JB-1, when administered to rainbow trout, Oncorhynchus mykiss, conferred protection against a new biogroup of Yersinia ruckeri. Thus, intraperitoneal or intramuscular injection of rainbow trout with cell wall proteins (CWPs), outer membrane proteins (OMPs), lipopolysaccharides (LPS), whole cell proteins (WCPs) and live cells followed by challenge on day 8 with Y. ruckeri led to 80-100% survival compared with 10% survival in the controls. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) profiles of WCPs and OMPs from GC2 had 10 and 5 variable protein bands in comparison to 11 and 5 bands in the WCPs and CWPs from JB-1. Proteomic analyses were employed following SDS-PAGE to categorize one dominant protein of 104.7 kDa from the CWPs of JB-1 and equated it with 'Bacillus spp. endoglucanase' with a Mascot score >69. These results point to the potential of using cellular components of probiotics for protection of fish against bacterial diseases.
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use of garlic allium sativum to control aeromonas hydrophila infection in rainbow trout Oncorhynchus mykiss walbaum
Journal of Fish Diseases, 2009Co-Authors: Elijah Jacob Nya, Brian AustinAbstract:Garlic, Allium sativum, which was fed at 0, 0.05, 0.1, 0.5 and 1.0 g per 100 g of feed for 14 days to rainbow trout, Oncorhynchus mykiss (Walbaum), led to control of experimental infection with Aeromonas hydrophila. At doses of 0.5 and 1.0 g garlic per 100 g of feed, there was a reduction in mortalities to 4% compared with the controls (88%). Moreover, there was a significant increase in growth, feed conversion and protein efficiency. There was stimulation of the number of erythrocytes and leucocytes, a significantly higher haematocrit, enhancement of phagocytic activity, respiratory burst, lysozyme, anti-protease and bactericidal activities following feeding with garlic.
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use of dietary ginger zingiber officinale roscoe as an immunostimulant to control aeromonas hydrophila infections in rainbow trout Oncorhynchus mykiss walbaum
Journal of Fish Diseases, 2009Co-Authors: Elijah Jacob Nya, Brian AustinAbstract:Ginger, Zingiber officinale, which was fed at 0, 0.05, 0.1, 0.5 and 1.0 g per 100 g of feed for 14 days to rainbow trout, Oncorhynchus mykiss (Walbaum), led to control of experimental infection with Aeromonas hydrophila. At 0.5 g ginger per 100 g of feed, there was a reduction in mortalities to 0% compared with the controls (64%). Moreover, there was a significant increase in growth, feed conversion and protein efficiency. There was proliferation in the number of neutrophils, macrophages and lymphocytes, and enhanced phagocytic, respiratory burst, lysozyme, bactericidal and anti-protease activities compared with the controls.
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Innate immune responses in rainbow trout (Oncorhynchus mykiss, Walbaum) induced by probiotics
Fish & shellfish immunology, 2006Co-Authors: Do-hyung Kim, Brian AustinAbstract:Abstract Carnobacterium maltaromaticum B26 and Carnobacterium divergens B33, which were isolated from the intestine of healthy rainbow trout (Oncorhynchus mykiss, Walbaum), were selected as being potentially useful as probiotics with effectiveness against Aeromonas salmonicida and Yersinia ruckeri . Thus, rainbow trout administered with feed supplemented with B26 or B33 dosed at >10 7 cells g −1 feed conferred protection against challenge with virulent cultures of the pathogens. Moreover, both cultures persisted in the gut for up to 3 weeks after administration. The cultures enhanced the cellular and humoral immune responses. Specifically, fish fed with B26 demonstrated significantly increased phagocytic activity of the head kidney macrophages, whereas the use of B33 led to significant increases in respiratory burst and serum lysozyme activity. Also, the gut mucosal lysozyme activity for fish fed with both cultures was statistically higher than the controls.
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use of a probiotic to control lactococcosis and streptococcosis in rainbow trout Oncorhynchus mykiss walbaum
Journal of Fish Diseases, 2005Co-Authors: Jason Brunt, Brian AustinAbstract:From a comparison of 125 bacterial isolates recovered from the digestive tract of rainbow trout, Oncorhynchus mykiss, and carp, Cyprinus sp., a culture was obtained which was effective at preventing clinical disease caused by Lactococcus garvieae and Streptococcus iniae when used as a feed additive. The culture, Aeromonas sobria GC2, was incorporated into the feed and fed to rainbow trout (average weight = 20 g) for 14 days at a dose equivalent to 5 x 10(7) cells g(-1) of feed. Whereas the untreated controls experienced losses of 75-100% when challenged intraperitoneally with L. garvieae and S. iniae, the probiotic-treated groups remained healthy with total mortalities of only 0-6%. Formalized and sonicated preparations of GC2 and cell-free supernatant fared less well. The mode of action reflected stimulation of innate immunity, namely an increased number of leucocytes and enhanced phagocytic and respiratory burst activity.
Kurt Buchmann - One of the best experts on this subject based on the ideXlab platform.
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rainbow trout Oncorhynchus mykiss immune response towards a recombinant vaccine targeting the parasitic ciliate ichthyophthirius multifiliis
Journal of Fish Diseases, 2017Co-Authors: Louise Von Gersdorff Jorgensen, Karina Juhl Rasmussen, Andreas Holm Mattsson, Azmi Aljubury, Per W Kania, Ali Salanti, Adam F Sander, Jan Schmidt, Kurt BuchmannAbstract:The protective effect in rainbow trout (Oncorhynchus mykiss) of an experimental subunit vaccine targeting antigens in the parasite Ichthyophthirius multifiliis has been evaluated and compared to effects elicited by a classical parasite homogenate vaccine. Three recombinant parasite proteins (two produced in E. coli and one in insect cells) were combined and injected i.p., and subsequently, protection and antibody responses were analysed. Both the experimental and the benchmark vaccine induced partial but significant protection against I. multifiliis when compared to control fish. Specific antibody responses of vaccinated trout (subunit vaccine) were raised against one neurohypophysial n-terminal domain protein #10 of three recombinant proteins, whereas the benchmark vaccine group showed specific antibody production against all three recombinant proteins. The immunogenic parasite protein #10 may be a potential vaccine candidate supplementing the protective I-antigen in future vaccine trials.
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rainbow trout Oncorhynchus mykiss immune response towards a recombinant vaccine targeting the parasitic ciliate ichthyophthirius multifiliis
Journal of Fish Diseases, 2017Co-Authors: Louise Von Gersdorff Jorgensen, Karina Juhl Rasmussen, Andreas Holm Mattsson, Azmi Aljubury, Per W Kania, Ali Salanti, Adam F Sander, Jan Schmidt, Kurt BuchmannAbstract:The protective effect in rainbow trout (Oncorhynchus mykiss) of an experimental subunit vaccine targeting antigens in the parasite Ichthyophthirius multifiliis has been evaluated and compared to effects elicited by a classical parasite homogenate vaccine. Three recombinant parasite proteins (two produced in E. coli and one in insect cells) were combined and injected i.p., and subsequently, protection and antibody responses were analysed. Both the experimental and the benchmark vaccine induced partial but significant protection against I. multifiliis when compared to control fish. Specific antibody responses of vaccinated trout (subunit vaccine) were raised against one neurohypophysial n-terminal domain protein #10 of three recombinant proteins, whereas the benchmark vaccine group showed specific antibody production against all three recombinant proteins. The immunogenic parasite protein #10 may be a potential vaccine candidate supplementing the protective I-antigen in future vaccine trials.
Louise Von Gersdorff Jorgensen - One of the best experts on this subject based on the ideXlab platform.
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rainbow trout Oncorhynchus mykiss immune response towards a recombinant vaccine targeting the parasitic ciliate ichthyophthirius multifiliis
Journal of Fish Diseases, 2017Co-Authors: Louise Von Gersdorff Jorgensen, Karina Juhl Rasmussen, Andreas Holm Mattsson, Azmi Aljubury, Per W Kania, Ali Salanti, Adam F Sander, Jan Schmidt, Kurt BuchmannAbstract:The protective effect in rainbow trout (Oncorhynchus mykiss) of an experimental subunit vaccine targeting antigens in the parasite Ichthyophthirius multifiliis has been evaluated and compared to effects elicited by a classical parasite homogenate vaccine. Three recombinant parasite proteins (two produced in E. coli and one in insect cells) were combined and injected i.p., and subsequently, protection and antibody responses were analysed. Both the experimental and the benchmark vaccine induced partial but significant protection against I. multifiliis when compared to control fish. Specific antibody responses of vaccinated trout (subunit vaccine) were raised against one neurohypophysial n-terminal domain protein #10 of three recombinant proteins, whereas the benchmark vaccine group showed specific antibody production against all three recombinant proteins. The immunogenic parasite protein #10 may be a potential vaccine candidate supplementing the protective I-antigen in future vaccine trials.
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rainbow trout Oncorhynchus mykiss immune response towards a recombinant vaccine targeting the parasitic ciliate ichthyophthirius multifiliis
Journal of Fish Diseases, 2017Co-Authors: Louise Von Gersdorff Jorgensen, Karina Juhl Rasmussen, Andreas Holm Mattsson, Azmi Aljubury, Per W Kania, Ali Salanti, Adam F Sander, Jan Schmidt, Kurt BuchmannAbstract:The protective effect in rainbow trout (Oncorhynchus mykiss) of an experimental subunit vaccine targeting antigens in the parasite Ichthyophthirius multifiliis has been evaluated and compared to effects elicited by a classical parasite homogenate vaccine. Three recombinant parasite proteins (two produced in E. coli and one in insect cells) were combined and injected i.p., and subsequently, protection and antibody responses were analysed. Both the experimental and the benchmark vaccine induced partial but significant protection against I. multifiliis when compared to control fish. Specific antibody responses of vaccinated trout (subunit vaccine) were raised against one neurohypophysial n-terminal domain protein #10 of three recombinant proteins, whereas the benchmark vaccine group showed specific antibody production against all three recombinant proteins. The immunogenic parasite protein #10 may be a potential vaccine candidate supplementing the protective I-antigen in future vaccine trials.
Jan Schmidt - One of the best experts on this subject based on the ideXlab platform.
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rainbow trout Oncorhynchus mykiss immune response towards a recombinant vaccine targeting the parasitic ciliate ichthyophthirius multifiliis
Journal of Fish Diseases, 2017Co-Authors: Louise Von Gersdorff Jorgensen, Karina Juhl Rasmussen, Andreas Holm Mattsson, Azmi Aljubury, Per W Kania, Ali Salanti, Adam F Sander, Jan Schmidt, Kurt BuchmannAbstract:The protective effect in rainbow trout (Oncorhynchus mykiss) of an experimental subunit vaccine targeting antigens in the parasite Ichthyophthirius multifiliis has been evaluated and compared to effects elicited by a classical parasite homogenate vaccine. Three recombinant parasite proteins (two produced in E. coli and one in insect cells) were combined and injected i.p., and subsequently, protection and antibody responses were analysed. Both the experimental and the benchmark vaccine induced partial but significant protection against I. multifiliis when compared to control fish. Specific antibody responses of vaccinated trout (subunit vaccine) were raised against one neurohypophysial n-terminal domain protein #10 of three recombinant proteins, whereas the benchmark vaccine group showed specific antibody production against all three recombinant proteins. The immunogenic parasite protein #10 may be a potential vaccine candidate supplementing the protective I-antigen in future vaccine trials.
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rainbow trout Oncorhynchus mykiss immune response towards a recombinant vaccine targeting the parasitic ciliate ichthyophthirius multifiliis
Journal of Fish Diseases, 2017Co-Authors: Louise Von Gersdorff Jorgensen, Karina Juhl Rasmussen, Andreas Holm Mattsson, Azmi Aljubury, Per W Kania, Ali Salanti, Adam F Sander, Jan Schmidt, Kurt BuchmannAbstract:The protective effect in rainbow trout (Oncorhynchus mykiss) of an experimental subunit vaccine targeting antigens in the parasite Ichthyophthirius multifiliis has been evaluated and compared to effects elicited by a classical parasite homogenate vaccine. Three recombinant parasite proteins (two produced in E. coli and one in insect cells) were combined and injected i.p., and subsequently, protection and antibody responses were analysed. Both the experimental and the benchmark vaccine induced partial but significant protection against I. multifiliis when compared to control fish. Specific antibody responses of vaccinated trout (subunit vaccine) were raised against one neurohypophysial n-terminal domain protein #10 of three recombinant proteins, whereas the benchmark vaccine group showed specific antibody production against all three recombinant proteins. The immunogenic parasite protein #10 may be a potential vaccine candidate supplementing the protective I-antigen in future vaccine trials.
Adam F Sander - One of the best experts on this subject based on the ideXlab platform.
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rainbow trout Oncorhynchus mykiss immune response towards a recombinant vaccine targeting the parasitic ciliate ichthyophthirius multifiliis
Journal of Fish Diseases, 2017Co-Authors: Louise Von Gersdorff Jorgensen, Karina Juhl Rasmussen, Andreas Holm Mattsson, Azmi Aljubury, Per W Kania, Ali Salanti, Adam F Sander, Jan Schmidt, Kurt BuchmannAbstract:The protective effect in rainbow trout (Oncorhynchus mykiss) of an experimental subunit vaccine targeting antigens in the parasite Ichthyophthirius multifiliis has been evaluated and compared to effects elicited by a classical parasite homogenate vaccine. Three recombinant parasite proteins (two produced in E. coli and one in insect cells) were combined and injected i.p., and subsequently, protection and antibody responses were analysed. Both the experimental and the benchmark vaccine induced partial but significant protection against I. multifiliis when compared to control fish. Specific antibody responses of vaccinated trout (subunit vaccine) were raised against one neurohypophysial n-terminal domain protein #10 of three recombinant proteins, whereas the benchmark vaccine group showed specific antibody production against all three recombinant proteins. The immunogenic parasite protein #10 may be a potential vaccine candidate supplementing the protective I-antigen in future vaccine trials.
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rainbow trout Oncorhynchus mykiss immune response towards a recombinant vaccine targeting the parasitic ciliate ichthyophthirius multifiliis
Journal of Fish Diseases, 2017Co-Authors: Louise Von Gersdorff Jorgensen, Karina Juhl Rasmussen, Andreas Holm Mattsson, Azmi Aljubury, Per W Kania, Ali Salanti, Adam F Sander, Jan Schmidt, Kurt BuchmannAbstract:The protective effect in rainbow trout (Oncorhynchus mykiss) of an experimental subunit vaccine targeting antigens in the parasite Ichthyophthirius multifiliis has been evaluated and compared to effects elicited by a classical parasite homogenate vaccine. Three recombinant parasite proteins (two produced in E. coli and one in insect cells) were combined and injected i.p., and subsequently, protection and antibody responses were analysed. Both the experimental and the benchmark vaccine induced partial but significant protection against I. multifiliis when compared to control fish. Specific antibody responses of vaccinated trout (subunit vaccine) were raised against one neurohypophysial n-terminal domain protein #10 of three recombinant proteins, whereas the benchmark vaccine group showed specific antibody production against all three recombinant proteins. The immunogenic parasite protein #10 may be a potential vaccine candidate supplementing the protective I-antigen in future vaccine trials.
