The Experts below are selected from a list of 45 Experts worldwide ranked by ideXlab platform
Liang Weihong - One of the best experts on this subject based on the ideXlab platform.
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localization analysis of gfp in Onion Epidermal Cell via agrobacterium tumefaciens mediated transformation
Journal of Henan Normal University, 2009Co-Authors: Liang WeihongAbstract:The plant transformation binary vector pCAMBIA1300-35S-GFP harboring a CaMV 35S promoter fused green fluorescent protein(GFP)reporter gene has been constructed,and transformed into Onion epidemal Cells by Agrobacterium-mediated method.Fluorescence microscope observation show that the reporter GFP gene expresses in Onion Epidermal Cells both in cytoplasm and nucleus.The result fasciliate the further study on subCellular localization and transient expression of new genes in Onion Epidermal Cells.
Qiu Fengya - One of the best experts on this subject based on the ideXlab platform.
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study on the influence ofdifferent treatment for cytoskeleton of pre immobilized Onion Epidermal Cell
Journal of Longyan University, 2014Co-Authors: Qiu FengyaAbstract:Optimal time of extracting non-cytoskeleton protein and the effect of dipping in carnoys fixative were studied.The material was pre-immobilized Onion inside and outside epidermial Cells.The cytoskeleton of epidermial Cell from different places in the pre-immobilized Onion and pre-immobilized Onion inner-epidermial Cell treated with different temperatures,UV-B radiation and colchicine were researched.Results showed that extracting non-cytoskeleton protein with 1% Triton X-100for 30min had a positive effect on the microscopic observation on cytoskeleton of the preimmobilized Onion inner-Epidermal Cell and for 25min in outer skin.Different maturity of scale leaves had an influence on cytoskeleton morphology and stability.Carnoys fixative has no effect on the cytoskeleton.
Yongxin Wang - One of the best experts on this subject based on the ideXlab platform.
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Construction of Plant Expression Vector with Maize γ-zein Gene and GFP Gene and Their SubCellular Localization
Maize Genomics and Genetics, 2012Co-Authors: Yu Zhang, Cong Li, Daxu Li, Yongchang Deng, Yongxin WangAbstract:The full length of the maize γ-zein gene amplified by PCR from PROK.TG1LK plasmid was ligated into the intermediate vector pMD18-T, to obtain the recombinant plasmid named pMD18-γ-zein. After the recombinant plasmid and the improved pCAMBIAI1302,respectively were digested by Nco I and Bgl II restriction endonucleases, the plant expression vector pCB-GFP-γ-zein with the gene of green fluorescent protein (GFP) driven by 35S was built following the procedures of recovery, ligation, transformation and identification. The fusion of GFP-γ-zein gene was integrated into the Onion Epidermal Cells by using the Biolistic approach; the fusion gene was expressed in Onion Epidermal Cell wall, nucleus and cytoplasm confirmed by confocal microscopy.
Xu Feng - One of the best experts on this subject based on the ideXlab platform.
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direct dna transformation of minimal linear smgfp gene cassette and its transient expression in Onion Epidermal Cell
Bulletin of Botanical Research, 2009Co-Authors: Xu FengAbstract:The minimal linear smGFP gene cassette was constructed and transiently expressed in Onion Epidermal Cell by adjusting the osmotic pressure of the transformation buffer. Strong fluorescence of green fluorescent protein was observed in cytoplasm and nucleus by fluorescence microscope, which proved that smGFP gene cassettes transformed into Onion Epidermal Cell and kept its integrity. Exogenous gene was penetrated into host Cell directly without any other force, such as particle bombardment, electransformation and microinjection. Therefore, it induces less damage to the host Cells and has a wide range of application. In addition, this method is easily operated and less costly.
Liu Xuan-ming - One of the best experts on this subject based on the ideXlab platform.
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Over-expression and function analysis of AKIN11 gene in Arabidopsis
Journal of Hunan Agricultural University, 2020Co-Authors: Liu Xuan-mingAbstract:The sucrose nonfermenting-1-related protein kinase(SnRK1) AKIN11 was inserted the downstream of the 35S promoter in the plant expression vector pEGAD and then was transformed to Onion Epidermal Cell by the particle bombardment.We found that the green fluorescent was focused on the nucleus.The recombination vector was introduced into Arabidopsis thaliana by Agrobacterium tumefaciens through the floral dip method.The two independent homozygous transgenic lines and T3 progenies were obtained by the Basta screening and RT-PCR method.Mutants have no distinguishable phenotype from that of the wild type.However,the starch content shows an increase in leaves of transgenic lines.RT-PCR analyses find that the AKIN11 gene affects the expression of the AGPase,which is a key enzyme in the pathway of the starch synthesis.In contrastt,here are no changes in expression of the AMY3.The results indicate that the AKIN11 gene may play an important role in the regulation of pathway of the starch accumulation in Arabidopsis thaliana.
