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Filippo Maria Ubaldi - One of the best experts on this subject based on the ideXlab platform.

  • embryo development of fresh versus vitrified metaphase ii Oocytes after icsi a prospective randomized sibling Oocyte study
    Human Reproduction, 2010
    Co-Authors: Laura Rienzi, Stefania Romano, Laura Albricci, Roberta Maggiulli, Antonio Capalbo, E Baroni, Silvia Colamaria, Fabio Sapienza, Filippo Maria Ubaldi
    Abstract:

    methods: In order to validate the effectiveness of Oocyte vitrification a non-inferiority trial was started on sibling metaphase II (MII) Oocytes. To demonstrate the non-inferiority based on an absolute difference of 17% in the fertilization rate per sibling Oocyte, a minimum of 222 Oocytes were required. After Oocyte denudation, MII Oocytes with normal morphology were randomly allocated to fresh ICSI insemination or to vitrification procedure. If pregnancy was not obtained a subsequent ICSI cycle was performed with warmed Oocytes of the same cohort. In both groups, three Oocytes were inseminated per cycle by ICSI procedure. Primary end-points were fertilization rates calculated per warmed and per injected Oocytes. Secondary end-points were zygote and embryo morphology. results: A total of 244 Oocytes were involved in this study. Of the 120 fresh sibling Oocytes inseminated, 100 were fertilized (83.3%). Survival rate of sibling vitrified Oocytes was 96.8% (120/124 Oocytes). Fertilization rate after ICSI was 76.6% (95/124) per warmed Oocyte and 79.2% (95/120) per survived/inseminated Oocyte. No statistical difference in fertilization rates was observed between the two groups when calculated per sibling Oocytes (absolute difference 26.73%; OR: 0.65; 95% CI ¼ 0.33 –1.29; P ¼ 0.20) and per inseminated Oocyte (absolute difference 24.17%; OR: 0.76; 95% CI ¼ 0.37 –1.53; P ¼ 0.50). Embryo development was also similar in both treatment groups up till Day 2. The percentage of excellent quality embryos was 52.0% (52/100) in the fresh group and 51.6% (49/95) in the vitrification group (absolute difference 20.43%; OR: 0.98; 95% CI ¼ 0.53 –1.79; P ¼ 0.9). The mean age of the 40 patients included in this study was 35.5+ 4.8 years (range 26– 42). Fifteen clinical pregnancies were obtained in the vitrification cycles of 39 embryo transfers performed (37.5% per cycle, 38.5% per embryo transfer), with an implantation rate of 20.2% (19/94). Three spontaneous miscarriages occurred (20%). Twelve pregnancies are ongoing (30.0% per cycle, 30.8% per embryo transfer) beyond 12 weeks of gestation. conclusions: Our results indicate that Oocyte vitrification procedure followed by ICSI is not inferior to fresh insemination procedure, with regard to fertilization and embryo developmental rates. Moreover, ongoing clinical pregnancy is compatible with this procedure, even with a restricted number of Oocytes available for insemination. The promising clinical results obtained, in a population of infertile patients, need to be confirmed on a larger scale. Clinical Trials Registration number: iSRCTN60158641.

  • embryo development of fresh versus vitrified metaphase ii Oocytes after icsi a prospective randomized sibling Oocyte study
    Human Reproduction, 2010
    Co-Authors: Laura Rienzi, Stefania Romano, Laura Albricci, Roberta Maggiulli, Antonio Capalbo, E Baroni, Silvia Colamaria, Fabio Sapienza, Filippo Maria Ubaldi
    Abstract:

    methods: In order to validate the effectiveness of Oocyte vitrification a non-inferiority trial was started on sibling metaphase II (MII) Oocytes. To demonstrate the non-inferiority based on an absolute difference of 17% in the fertilization rate per sibling Oocyte, a minimum of 222 Oocytes were required. After Oocyte denudation, MII Oocytes with normal morphology were randomly allocated to fresh ICSI insemination or to vitrification procedure. If pregnancy was not obtained a subsequent ICSI cycle was performed with warmed Oocytes of the same cohort. In both groups, three Oocytes were inseminated per cycle by ICSI procedure. Primary end-points were fertilization rates calculated per warmed and per injected Oocytes. Secondary end-points were zygote and embryo morphology. results: A total of 244 Oocytes were involved in this study. Of the 120 fresh sibling Oocytes inseminated, 100 were fertilized (83.3%). Survival rate of sibling vitrified Oocytes was 96.8% (120/124 Oocytes). Fertilization rate after ICSI was 76.6% (95/124) per warmed Oocyte and 79.2% (95/120) per survived/inseminated Oocyte. No statistical difference in fertilization rates was observed between the two groups when calculated per sibling Oocytes (absolute difference 26.73%; OR: 0.65; 95% CI ¼ 0.33 –1.29; P ¼ 0.20) and per inseminated Oocyte (absolute difference 24.17%; OR: 0.76; 95% CI ¼ 0.37 –1.53; P ¼ 0.50). Embryo development was also similar in both treatment groups up till Day 2. The percentage of excellent quality embryos was 52.0% (52/100) in the fresh group and 51.6% (49/95) in the vitrification group (absolute difference 20.43%; OR: 0.98; 95% CI ¼ 0.53 –1.79; P ¼ 0.9). The mean age of the 40 patients included in this study was 35.5+ 4.8 years (range 26– 42). Fifteen clinical pregnancies were obtained in the vitrification cycles of 39 embryo transfers performed (37.5% per cycle, 38.5% per embryo transfer), with an implantation rate of 20.2% (19/94). Three spontaneous miscarriages occurred (20%). Twelve pregnancies are ongoing (30.0% per cycle, 30.8% per embryo transfer) beyond 12 weeks of gestation. conclusions: Our results indicate that Oocyte vitrification procedure followed by ICSI is not inferior to fresh insemination procedure, with regard to fertilization and embryo developmental rates. Moreover, ongoing clinical pregnancy is compatible with this procedure, even with a restricted number of Oocytes available for insemination. The promising clinical results obtained, in a population of infertile patients, need to be confirmed on a larger scale. Clinical Trials Registration number: iSRCTN60158641.

Laura Rienzi - One of the best experts on this subject based on the ideXlab platform.

  • embryo development of fresh versus vitrified metaphase ii Oocytes after icsi a prospective randomized sibling Oocyte study
    Human Reproduction, 2010
    Co-Authors: Laura Rienzi, Stefania Romano, Laura Albricci, Roberta Maggiulli, Antonio Capalbo, E Baroni, Silvia Colamaria, Fabio Sapienza, Filippo Maria Ubaldi
    Abstract:

    methods: In order to validate the effectiveness of Oocyte vitrification a non-inferiority trial was started on sibling metaphase II (MII) Oocytes. To demonstrate the non-inferiority based on an absolute difference of 17% in the fertilization rate per sibling Oocyte, a minimum of 222 Oocytes were required. After Oocyte denudation, MII Oocytes with normal morphology were randomly allocated to fresh ICSI insemination or to vitrification procedure. If pregnancy was not obtained a subsequent ICSI cycle was performed with warmed Oocytes of the same cohort. In both groups, three Oocytes were inseminated per cycle by ICSI procedure. Primary end-points were fertilization rates calculated per warmed and per injected Oocytes. Secondary end-points were zygote and embryo morphology. results: A total of 244 Oocytes were involved in this study. Of the 120 fresh sibling Oocytes inseminated, 100 were fertilized (83.3%). Survival rate of sibling vitrified Oocytes was 96.8% (120/124 Oocytes). Fertilization rate after ICSI was 76.6% (95/124) per warmed Oocyte and 79.2% (95/120) per survived/inseminated Oocyte. No statistical difference in fertilization rates was observed between the two groups when calculated per sibling Oocytes (absolute difference 26.73%; OR: 0.65; 95% CI ¼ 0.33 –1.29; P ¼ 0.20) and per inseminated Oocyte (absolute difference 24.17%; OR: 0.76; 95% CI ¼ 0.37 –1.53; P ¼ 0.50). Embryo development was also similar in both treatment groups up till Day 2. The percentage of excellent quality embryos was 52.0% (52/100) in the fresh group and 51.6% (49/95) in the vitrification group (absolute difference 20.43%; OR: 0.98; 95% CI ¼ 0.53 –1.79; P ¼ 0.9). The mean age of the 40 patients included in this study was 35.5+ 4.8 years (range 26– 42). Fifteen clinical pregnancies were obtained in the vitrification cycles of 39 embryo transfers performed (37.5% per cycle, 38.5% per embryo transfer), with an implantation rate of 20.2% (19/94). Three spontaneous miscarriages occurred (20%). Twelve pregnancies are ongoing (30.0% per cycle, 30.8% per embryo transfer) beyond 12 weeks of gestation. conclusions: Our results indicate that Oocyte vitrification procedure followed by ICSI is not inferior to fresh insemination procedure, with regard to fertilization and embryo developmental rates. Moreover, ongoing clinical pregnancy is compatible with this procedure, even with a restricted number of Oocytes available for insemination. The promising clinical results obtained, in a population of infertile patients, need to be confirmed on a larger scale. Clinical Trials Registration number: iSRCTN60158641.

  • embryo development of fresh versus vitrified metaphase ii Oocytes after icsi a prospective randomized sibling Oocyte study
    Human Reproduction, 2010
    Co-Authors: Laura Rienzi, Stefania Romano, Laura Albricci, Roberta Maggiulli, Antonio Capalbo, E Baroni, Silvia Colamaria, Fabio Sapienza, Filippo Maria Ubaldi
    Abstract:

    methods: In order to validate the effectiveness of Oocyte vitrification a non-inferiority trial was started on sibling metaphase II (MII) Oocytes. To demonstrate the non-inferiority based on an absolute difference of 17% in the fertilization rate per sibling Oocyte, a minimum of 222 Oocytes were required. After Oocyte denudation, MII Oocytes with normal morphology were randomly allocated to fresh ICSI insemination or to vitrification procedure. If pregnancy was not obtained a subsequent ICSI cycle was performed with warmed Oocytes of the same cohort. In both groups, three Oocytes were inseminated per cycle by ICSI procedure. Primary end-points were fertilization rates calculated per warmed and per injected Oocytes. Secondary end-points were zygote and embryo morphology. results: A total of 244 Oocytes were involved in this study. Of the 120 fresh sibling Oocytes inseminated, 100 were fertilized (83.3%). Survival rate of sibling vitrified Oocytes was 96.8% (120/124 Oocytes). Fertilization rate after ICSI was 76.6% (95/124) per warmed Oocyte and 79.2% (95/120) per survived/inseminated Oocyte. No statistical difference in fertilization rates was observed between the two groups when calculated per sibling Oocytes (absolute difference 26.73%; OR: 0.65; 95% CI ¼ 0.33 –1.29; P ¼ 0.20) and per inseminated Oocyte (absolute difference 24.17%; OR: 0.76; 95% CI ¼ 0.37 –1.53; P ¼ 0.50). Embryo development was also similar in both treatment groups up till Day 2. The percentage of excellent quality embryos was 52.0% (52/100) in the fresh group and 51.6% (49/95) in the vitrification group (absolute difference 20.43%; OR: 0.98; 95% CI ¼ 0.53 –1.79; P ¼ 0.9). The mean age of the 40 patients included in this study was 35.5+ 4.8 years (range 26– 42). Fifteen clinical pregnancies were obtained in the vitrification cycles of 39 embryo transfers performed (37.5% per cycle, 38.5% per embryo transfer), with an implantation rate of 20.2% (19/94). Three spontaneous miscarriages occurred (20%). Twelve pregnancies are ongoing (30.0% per cycle, 30.8% per embryo transfer) beyond 12 weeks of gestation. conclusions: Our results indicate that Oocyte vitrification procedure followed by ICSI is not inferior to fresh insemination procedure, with regard to fertilization and embryo developmental rates. Moreover, ongoing clinical pregnancy is compatible with this procedure, even with a restricted number of Oocytes available for insemination. The promising clinical results obtained, in a population of infertile patients, need to be confirmed on a larger scale. Clinical Trials Registration number: iSRCTN60158641.

Stefania Romano - One of the best experts on this subject based on the ideXlab platform.

  • embryo development of fresh versus vitrified metaphase ii Oocytes after icsi a prospective randomized sibling Oocyte study
    Human Reproduction, 2010
    Co-Authors: Laura Rienzi, Stefania Romano, Laura Albricci, Roberta Maggiulli, Antonio Capalbo, E Baroni, Silvia Colamaria, Fabio Sapienza, Filippo Maria Ubaldi
    Abstract:

    methods: In order to validate the effectiveness of Oocyte vitrification a non-inferiority trial was started on sibling metaphase II (MII) Oocytes. To demonstrate the non-inferiority based on an absolute difference of 17% in the fertilization rate per sibling Oocyte, a minimum of 222 Oocytes were required. After Oocyte denudation, MII Oocytes with normal morphology were randomly allocated to fresh ICSI insemination or to vitrification procedure. If pregnancy was not obtained a subsequent ICSI cycle was performed with warmed Oocytes of the same cohort. In both groups, three Oocytes were inseminated per cycle by ICSI procedure. Primary end-points were fertilization rates calculated per warmed and per injected Oocytes. Secondary end-points were zygote and embryo morphology. results: A total of 244 Oocytes were involved in this study. Of the 120 fresh sibling Oocytes inseminated, 100 were fertilized (83.3%). Survival rate of sibling vitrified Oocytes was 96.8% (120/124 Oocytes). Fertilization rate after ICSI was 76.6% (95/124) per warmed Oocyte and 79.2% (95/120) per survived/inseminated Oocyte. No statistical difference in fertilization rates was observed between the two groups when calculated per sibling Oocytes (absolute difference 26.73%; OR: 0.65; 95% CI ¼ 0.33 –1.29; P ¼ 0.20) and per inseminated Oocyte (absolute difference 24.17%; OR: 0.76; 95% CI ¼ 0.37 –1.53; P ¼ 0.50). Embryo development was also similar in both treatment groups up till Day 2. The percentage of excellent quality embryos was 52.0% (52/100) in the fresh group and 51.6% (49/95) in the vitrification group (absolute difference 20.43%; OR: 0.98; 95% CI ¼ 0.53 –1.79; P ¼ 0.9). The mean age of the 40 patients included in this study was 35.5+ 4.8 years (range 26– 42). Fifteen clinical pregnancies were obtained in the vitrification cycles of 39 embryo transfers performed (37.5% per cycle, 38.5% per embryo transfer), with an implantation rate of 20.2% (19/94). Three spontaneous miscarriages occurred (20%). Twelve pregnancies are ongoing (30.0% per cycle, 30.8% per embryo transfer) beyond 12 weeks of gestation. conclusions: Our results indicate that Oocyte vitrification procedure followed by ICSI is not inferior to fresh insemination procedure, with regard to fertilization and embryo developmental rates. Moreover, ongoing clinical pregnancy is compatible with this procedure, even with a restricted number of Oocytes available for insemination. The promising clinical results obtained, in a population of infertile patients, need to be confirmed on a larger scale. Clinical Trials Registration number: iSRCTN60158641.

  • embryo development of fresh versus vitrified metaphase ii Oocytes after icsi a prospective randomized sibling Oocyte study
    Human Reproduction, 2010
    Co-Authors: Laura Rienzi, Stefania Romano, Laura Albricci, Roberta Maggiulli, Antonio Capalbo, E Baroni, Silvia Colamaria, Fabio Sapienza, Filippo Maria Ubaldi
    Abstract:

    methods: In order to validate the effectiveness of Oocyte vitrification a non-inferiority trial was started on sibling metaphase II (MII) Oocytes. To demonstrate the non-inferiority based on an absolute difference of 17% in the fertilization rate per sibling Oocyte, a minimum of 222 Oocytes were required. After Oocyte denudation, MII Oocytes with normal morphology were randomly allocated to fresh ICSI insemination or to vitrification procedure. If pregnancy was not obtained a subsequent ICSI cycle was performed with warmed Oocytes of the same cohort. In both groups, three Oocytes were inseminated per cycle by ICSI procedure. Primary end-points were fertilization rates calculated per warmed and per injected Oocytes. Secondary end-points were zygote and embryo morphology. results: A total of 244 Oocytes were involved in this study. Of the 120 fresh sibling Oocytes inseminated, 100 were fertilized (83.3%). Survival rate of sibling vitrified Oocytes was 96.8% (120/124 Oocytes). Fertilization rate after ICSI was 76.6% (95/124) per warmed Oocyte and 79.2% (95/120) per survived/inseminated Oocyte. No statistical difference in fertilization rates was observed between the two groups when calculated per sibling Oocytes (absolute difference 26.73%; OR: 0.65; 95% CI ¼ 0.33 –1.29; P ¼ 0.20) and per inseminated Oocyte (absolute difference 24.17%; OR: 0.76; 95% CI ¼ 0.37 –1.53; P ¼ 0.50). Embryo development was also similar in both treatment groups up till Day 2. The percentage of excellent quality embryos was 52.0% (52/100) in the fresh group and 51.6% (49/95) in the vitrification group (absolute difference 20.43%; OR: 0.98; 95% CI ¼ 0.53 –1.79; P ¼ 0.9). The mean age of the 40 patients included in this study was 35.5+ 4.8 years (range 26– 42). Fifteen clinical pregnancies were obtained in the vitrification cycles of 39 embryo transfers performed (37.5% per cycle, 38.5% per embryo transfer), with an implantation rate of 20.2% (19/94). Three spontaneous miscarriages occurred (20%). Twelve pregnancies are ongoing (30.0% per cycle, 30.8% per embryo transfer) beyond 12 weeks of gestation. conclusions: Our results indicate that Oocyte vitrification procedure followed by ICSI is not inferior to fresh insemination procedure, with regard to fertilization and embryo developmental rates. Moreover, ongoing clinical pregnancy is compatible with this procedure, even with a restricted number of Oocytes available for insemination. The promising clinical results obtained, in a population of infertile patients, need to be confirmed on a larger scale. Clinical Trials Registration number: iSRCTN60158641.

Laura Albricci - One of the best experts on this subject based on the ideXlab platform.

  • embryo development of fresh versus vitrified metaphase ii Oocytes after icsi a prospective randomized sibling Oocyte study
    Human Reproduction, 2010
    Co-Authors: Laura Rienzi, Stefania Romano, Laura Albricci, Roberta Maggiulli, Antonio Capalbo, E Baroni, Silvia Colamaria, Fabio Sapienza, Filippo Maria Ubaldi
    Abstract:

    methods: In order to validate the effectiveness of Oocyte vitrification a non-inferiority trial was started on sibling metaphase II (MII) Oocytes. To demonstrate the non-inferiority based on an absolute difference of 17% in the fertilization rate per sibling Oocyte, a minimum of 222 Oocytes were required. After Oocyte denudation, MII Oocytes with normal morphology were randomly allocated to fresh ICSI insemination or to vitrification procedure. If pregnancy was not obtained a subsequent ICSI cycle was performed with warmed Oocytes of the same cohort. In both groups, three Oocytes were inseminated per cycle by ICSI procedure. Primary end-points were fertilization rates calculated per warmed and per injected Oocytes. Secondary end-points were zygote and embryo morphology. results: A total of 244 Oocytes were involved in this study. Of the 120 fresh sibling Oocytes inseminated, 100 were fertilized (83.3%). Survival rate of sibling vitrified Oocytes was 96.8% (120/124 Oocytes). Fertilization rate after ICSI was 76.6% (95/124) per warmed Oocyte and 79.2% (95/120) per survived/inseminated Oocyte. No statistical difference in fertilization rates was observed between the two groups when calculated per sibling Oocytes (absolute difference 26.73%; OR: 0.65; 95% CI ¼ 0.33 –1.29; P ¼ 0.20) and per inseminated Oocyte (absolute difference 24.17%; OR: 0.76; 95% CI ¼ 0.37 –1.53; P ¼ 0.50). Embryo development was also similar in both treatment groups up till Day 2. The percentage of excellent quality embryos was 52.0% (52/100) in the fresh group and 51.6% (49/95) in the vitrification group (absolute difference 20.43%; OR: 0.98; 95% CI ¼ 0.53 –1.79; P ¼ 0.9). The mean age of the 40 patients included in this study was 35.5+ 4.8 years (range 26– 42). Fifteen clinical pregnancies were obtained in the vitrification cycles of 39 embryo transfers performed (37.5% per cycle, 38.5% per embryo transfer), with an implantation rate of 20.2% (19/94). Three spontaneous miscarriages occurred (20%). Twelve pregnancies are ongoing (30.0% per cycle, 30.8% per embryo transfer) beyond 12 weeks of gestation. conclusions: Our results indicate that Oocyte vitrification procedure followed by ICSI is not inferior to fresh insemination procedure, with regard to fertilization and embryo developmental rates. Moreover, ongoing clinical pregnancy is compatible with this procedure, even with a restricted number of Oocytes available for insemination. The promising clinical results obtained, in a population of infertile patients, need to be confirmed on a larger scale. Clinical Trials Registration number: iSRCTN60158641.

  • embryo development of fresh versus vitrified metaphase ii Oocytes after icsi a prospective randomized sibling Oocyte study
    Human Reproduction, 2010
    Co-Authors: Laura Rienzi, Stefania Romano, Laura Albricci, Roberta Maggiulli, Antonio Capalbo, E Baroni, Silvia Colamaria, Fabio Sapienza, Filippo Maria Ubaldi
    Abstract:

    methods: In order to validate the effectiveness of Oocyte vitrification a non-inferiority trial was started on sibling metaphase II (MII) Oocytes. To demonstrate the non-inferiority based on an absolute difference of 17% in the fertilization rate per sibling Oocyte, a minimum of 222 Oocytes were required. After Oocyte denudation, MII Oocytes with normal morphology were randomly allocated to fresh ICSI insemination or to vitrification procedure. If pregnancy was not obtained a subsequent ICSI cycle was performed with warmed Oocytes of the same cohort. In both groups, three Oocytes were inseminated per cycle by ICSI procedure. Primary end-points were fertilization rates calculated per warmed and per injected Oocytes. Secondary end-points were zygote and embryo morphology. results: A total of 244 Oocytes were involved in this study. Of the 120 fresh sibling Oocytes inseminated, 100 were fertilized (83.3%). Survival rate of sibling vitrified Oocytes was 96.8% (120/124 Oocytes). Fertilization rate after ICSI was 76.6% (95/124) per warmed Oocyte and 79.2% (95/120) per survived/inseminated Oocyte. No statistical difference in fertilization rates was observed between the two groups when calculated per sibling Oocytes (absolute difference 26.73%; OR: 0.65; 95% CI ¼ 0.33 –1.29; P ¼ 0.20) and per inseminated Oocyte (absolute difference 24.17%; OR: 0.76; 95% CI ¼ 0.37 –1.53; P ¼ 0.50). Embryo development was also similar in both treatment groups up till Day 2. The percentage of excellent quality embryos was 52.0% (52/100) in the fresh group and 51.6% (49/95) in the vitrification group (absolute difference 20.43%; OR: 0.98; 95% CI ¼ 0.53 –1.79; P ¼ 0.9). The mean age of the 40 patients included in this study was 35.5+ 4.8 years (range 26– 42). Fifteen clinical pregnancies were obtained in the vitrification cycles of 39 embryo transfers performed (37.5% per cycle, 38.5% per embryo transfer), with an implantation rate of 20.2% (19/94). Three spontaneous miscarriages occurred (20%). Twelve pregnancies are ongoing (30.0% per cycle, 30.8% per embryo transfer) beyond 12 weeks of gestation. conclusions: Our results indicate that Oocyte vitrification procedure followed by ICSI is not inferior to fresh insemination procedure, with regard to fertilization and embryo developmental rates. Moreover, ongoing clinical pregnancy is compatible with this procedure, even with a restricted number of Oocytes available for insemination. The promising clinical results obtained, in a population of infertile patients, need to be confirmed on a larger scale. Clinical Trials Registration number: iSRCTN60158641.

Fabio Sapienza - One of the best experts on this subject based on the ideXlab platform.

  • embryo development of fresh versus vitrified metaphase ii Oocytes after icsi a prospective randomized sibling Oocyte study
    Human Reproduction, 2010
    Co-Authors: Laura Rienzi, Stefania Romano, Laura Albricci, Roberta Maggiulli, Antonio Capalbo, E Baroni, Silvia Colamaria, Fabio Sapienza, Filippo Maria Ubaldi
    Abstract:

    methods: In order to validate the effectiveness of Oocyte vitrification a non-inferiority trial was started on sibling metaphase II (MII) Oocytes. To demonstrate the non-inferiority based on an absolute difference of 17% in the fertilization rate per sibling Oocyte, a minimum of 222 Oocytes were required. After Oocyte denudation, MII Oocytes with normal morphology were randomly allocated to fresh ICSI insemination or to vitrification procedure. If pregnancy was not obtained a subsequent ICSI cycle was performed with warmed Oocytes of the same cohort. In both groups, three Oocytes were inseminated per cycle by ICSI procedure. Primary end-points were fertilization rates calculated per warmed and per injected Oocytes. Secondary end-points were zygote and embryo morphology. results: A total of 244 Oocytes were involved in this study. Of the 120 fresh sibling Oocytes inseminated, 100 were fertilized (83.3%). Survival rate of sibling vitrified Oocytes was 96.8% (120/124 Oocytes). Fertilization rate after ICSI was 76.6% (95/124) per warmed Oocyte and 79.2% (95/120) per survived/inseminated Oocyte. No statistical difference in fertilization rates was observed between the two groups when calculated per sibling Oocytes (absolute difference 26.73%; OR: 0.65; 95% CI ¼ 0.33 –1.29; P ¼ 0.20) and per inseminated Oocyte (absolute difference 24.17%; OR: 0.76; 95% CI ¼ 0.37 –1.53; P ¼ 0.50). Embryo development was also similar in both treatment groups up till Day 2. The percentage of excellent quality embryos was 52.0% (52/100) in the fresh group and 51.6% (49/95) in the vitrification group (absolute difference 20.43%; OR: 0.98; 95% CI ¼ 0.53 –1.79; P ¼ 0.9). The mean age of the 40 patients included in this study was 35.5+ 4.8 years (range 26– 42). Fifteen clinical pregnancies were obtained in the vitrification cycles of 39 embryo transfers performed (37.5% per cycle, 38.5% per embryo transfer), with an implantation rate of 20.2% (19/94). Three spontaneous miscarriages occurred (20%). Twelve pregnancies are ongoing (30.0% per cycle, 30.8% per embryo transfer) beyond 12 weeks of gestation. conclusions: Our results indicate that Oocyte vitrification procedure followed by ICSI is not inferior to fresh insemination procedure, with regard to fertilization and embryo developmental rates. Moreover, ongoing clinical pregnancy is compatible with this procedure, even with a restricted number of Oocytes available for insemination. The promising clinical results obtained, in a population of infertile patients, need to be confirmed on a larger scale. Clinical Trials Registration number: iSRCTN60158641.

  • embryo development of fresh versus vitrified metaphase ii Oocytes after icsi a prospective randomized sibling Oocyte study
    Human Reproduction, 2010
    Co-Authors: Laura Rienzi, Stefania Romano, Laura Albricci, Roberta Maggiulli, Antonio Capalbo, E Baroni, Silvia Colamaria, Fabio Sapienza, Filippo Maria Ubaldi
    Abstract:

    methods: In order to validate the effectiveness of Oocyte vitrification a non-inferiority trial was started on sibling metaphase II (MII) Oocytes. To demonstrate the non-inferiority based on an absolute difference of 17% in the fertilization rate per sibling Oocyte, a minimum of 222 Oocytes were required. After Oocyte denudation, MII Oocytes with normal morphology were randomly allocated to fresh ICSI insemination or to vitrification procedure. If pregnancy was not obtained a subsequent ICSI cycle was performed with warmed Oocytes of the same cohort. In both groups, three Oocytes were inseminated per cycle by ICSI procedure. Primary end-points were fertilization rates calculated per warmed and per injected Oocytes. Secondary end-points were zygote and embryo morphology. results: A total of 244 Oocytes were involved in this study. Of the 120 fresh sibling Oocytes inseminated, 100 were fertilized (83.3%). Survival rate of sibling vitrified Oocytes was 96.8% (120/124 Oocytes). Fertilization rate after ICSI was 76.6% (95/124) per warmed Oocyte and 79.2% (95/120) per survived/inseminated Oocyte. No statistical difference in fertilization rates was observed between the two groups when calculated per sibling Oocytes (absolute difference 26.73%; OR: 0.65; 95% CI ¼ 0.33 –1.29; P ¼ 0.20) and per inseminated Oocyte (absolute difference 24.17%; OR: 0.76; 95% CI ¼ 0.37 –1.53; P ¼ 0.50). Embryo development was also similar in both treatment groups up till Day 2. The percentage of excellent quality embryos was 52.0% (52/100) in the fresh group and 51.6% (49/95) in the vitrification group (absolute difference 20.43%; OR: 0.98; 95% CI ¼ 0.53 –1.79; P ¼ 0.9). The mean age of the 40 patients included in this study was 35.5+ 4.8 years (range 26– 42). Fifteen clinical pregnancies were obtained in the vitrification cycles of 39 embryo transfers performed (37.5% per cycle, 38.5% per embryo transfer), with an implantation rate of 20.2% (19/94). Three spontaneous miscarriages occurred (20%). Twelve pregnancies are ongoing (30.0% per cycle, 30.8% per embryo transfer) beyond 12 weeks of gestation. conclusions: Our results indicate that Oocyte vitrification procedure followed by ICSI is not inferior to fresh insemination procedure, with regard to fertilization and embryo developmental rates. Moreover, ongoing clinical pregnancy is compatible with this procedure, even with a restricted number of Oocytes available for insemination. The promising clinical results obtained, in a population of infertile patients, need to be confirmed on a larger scale. Clinical Trials Registration number: iSRCTN60158641.