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Bing Jiang - One of the best experts on this subject based on the ideXlab platform.

  • protective effects of human umbilical cord blood stem cell intravitreal transplantation against Optic Nerve Injury in rats
    Graefes Archive for Clinical and Experimental Ophthalmology, 2011
    Co-Authors: Tantai Zhao, Luosheng Tang, Fang Fan, Bing Jiang
    Abstract:

    Background The majority of studies addressing traumatic Optic neuropathy (TON) have focused on drugs, proteins, cytokines, and various surgical techniques. A recent study reported that transplantation of human umbilical cord blood stem cells (hUCBSCs) achieved therapeutic effects on TON, but the exact effects on Optic Nerve Injury are still unknown, and the mechanisms underlying Nerve protection remain poorly understood. Methods A total of 135 healthy Sprague–Dawley adult rats were randomly assigned to three groups: sham-surgery, model and transplantation, with 45 rats in each group. TON was induced in the model and transplantation groups via Optic Nerve crush Injury. The crush Injury was not performed in the sham-surgery group. Seven days after the Injury, 10 6 hUCBSCs were injected into the rat vitreous cavity of transplantation group, and an equal volume of physiological saline was administered to the model and sham-surgery groups. Pathological observation of rat retina tissues was performed by hematoxylin–eosin (H&E) staining at days 3, 7, 14, 21 and 28 post-surgery. The number of retinal ganglion cells (RGCs) and mRNA expression levels of brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) were assessed by the Fluorogold (FG) retrograde labeling and reverse transcriptase–polymerase chain reaction (RT–PCR) methods, respectively. Results The number of labeled RGCs and the expression of BDNF and GDNF mRNA obviously increased, and pathological Injury was significantly ameliorated in the transplantation group compared to the model group (P<0.05). Conclusions Via intravitreal transplantation, the hUCBSCs resulted in a significant increase in the survival of the RGCs, and improved pathological changes in the rat retina, following TON. The protective mechanism is correlated with the continuous secretion of BDNF and GDNF in vivo of retina in Optic Nerve Injury rats by the transplanted hUCBSCs.

Tantai Zhao - One of the best experts on this subject based on the ideXlab platform.

  • protective effects of human umbilical cord blood stem cell intravitreal transplantation against Optic Nerve Injury in rats
    Graefes Archive for Clinical and Experimental Ophthalmology, 2011
    Co-Authors: Tantai Zhao, Luosheng Tang, Fang Fan, Bing Jiang
    Abstract:

    Background The majority of studies addressing traumatic Optic neuropathy (TON) have focused on drugs, proteins, cytokines, and various surgical techniques. A recent study reported that transplantation of human umbilical cord blood stem cells (hUCBSCs) achieved therapeutic effects on TON, but the exact effects on Optic Nerve Injury are still unknown, and the mechanisms underlying Nerve protection remain poorly understood. Methods A total of 135 healthy Sprague–Dawley adult rats were randomly assigned to three groups: sham-surgery, model and transplantation, with 45 rats in each group. TON was induced in the model and transplantation groups via Optic Nerve crush Injury. The crush Injury was not performed in the sham-surgery group. Seven days after the Injury, 10 6 hUCBSCs were injected into the rat vitreous cavity of transplantation group, and an equal volume of physiological saline was administered to the model and sham-surgery groups. Pathological observation of rat retina tissues was performed by hematoxylin–eosin (H&E) staining at days 3, 7, 14, 21 and 28 post-surgery. The number of retinal ganglion cells (RGCs) and mRNA expression levels of brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) were assessed by the Fluorogold (FG) retrograde labeling and reverse transcriptase–polymerase chain reaction (RT–PCR) methods, respectively. Results The number of labeled RGCs and the expression of BDNF and GDNF mRNA obviously increased, and pathological Injury was significantly ameliorated in the transplantation group compared to the model group (P<0.05). Conclusions Via intravitreal transplantation, the hUCBSCs resulted in a significant increase in the survival of the RGCs, and improved pathological changes in the rat retina, following TON. The protective mechanism is correlated with the continuous secretion of BDNF and GDNF in vivo of retina in Optic Nerve Injury rats by the transplanted hUCBSCs.

Hilda Petrs-silva - One of the best experts on this subject based on the ideXlab platform.

  • Effects of a combinatorial treatment with gene and cell therapy on retinal ganglion cell survival and axonal outgrowth after Optic Nerve Injury
    Gene Therapy, 2020
    Co-Authors: Gabriel Nascimento-dos-santos, Leandro Coelho Teixeira-pinheiro, Almir Jordão Silva-júnior, Luiza Rachel Pinheiro De Carvalho, Louise Alessandra Mesentier-louro, William W. Hauswirth, Rosalia Mendez-otero, Marcelo Felippe Santiago, Hilda Petrs-silva
    Abstract:

    After an Injury, axons in the central nervous system do not regenerate over large distances and permanently lose their connections to the brain. Two promising approaches to correct this condition are cell and gene therapies. In the present work, we evaluated the neuroprotective and neuroregenerative potential of pigment epithelium-derived factor (PEDF) gene therapy alone and combined with human mesenchymal stem cell (hMSC) therapy after Optic Nerve Injury by analysis of retinal ganglion cell survival and axonal outgrowth. Overexpression of PEDF by intravitreal delivery of AAV2 vector significantly increased Tuj1-positive cells survival and modulated FGF-2, IL-1ß, Iba-1, and GFAP immunostaining in the ganglion cell layer (GCL) at 4 weeks after Optic Nerve crush, although it could not promote axonal outgrowth. The combination of AAV2.PEDF and hMSC therapy showed a higher number of Tuj1-positive cells and a pronounced axonal outgrowth than unimodal therapy after Optic Nerve crush. In summary, our results highlight a synergistic effect of combined gene and cell therapy relevant for future therapeutic interventions regarding Optic Nerve Injury.

  • Effects of a combinatorial treatment with gene and cell therapy on retinal ganglion cell survival and axonal outgrowth after Optic Nerve Injury
    Gene Therapy, 2020
    Co-Authors: Gabriel Nascimento-dos-santos, Leandro Coelho Teixeira-pinheiro, Almir Jordão Silva-júnior, Luiza Rachel Pinheiro De Carvalho, Louise Alessandra Mesentier-louro, William W. Hauswirth, Rosalia Mendez-otero, Marcelo Felippe Santiago, Hilda Petrs-silva
    Abstract:

    After an Injury, axons in the central nervous system do not regenerate over large distances and permanently lose their connections to the brain. Two promising approaches to correct this condition are cell and gene therapies. In the present work, we evaluated the neuroprotective and neuroregenerative potential of pigment epithelium-derived factor (PEDF) gene therapy alone and combined with human mesenchymal stem cell (hMSC) therapy after Optic Nerve Injury by analysis of retinal ganglion cell survival and axonal outgrowth. Overexpression of PEDF by intravitreal delivery of AAV2 vector significantly increased Tuj1-positive cells survival and modulated FGF-2, IL-1ß, Iba-1, and GFAP immunostaining in the ganglion cell layer (GCL) at 4 weeks after Optic Nerve crush, although it could not promote axonal outgrowth. The combination of AAV2.PEDF and hMSC therapy showed a higher number of Tuj1-positive cells and a pronounced axonal outgrowth than unimodal therapy after Optic Nerve crush. In summary, our results highlight a synergistic effect of combined gene and cell therapy relevant for future therapeutic interventions regarding Optic Nerve Injury.

Fang Fan - One of the best experts on this subject based on the ideXlab platform.

  • protective effects of human umbilical cord blood stem cell intravitreal transplantation against Optic Nerve Injury in rats
    Graefes Archive for Clinical and Experimental Ophthalmology, 2011
    Co-Authors: Tantai Zhao, Luosheng Tang, Fang Fan, Bing Jiang
    Abstract:

    Background The majority of studies addressing traumatic Optic neuropathy (TON) have focused on drugs, proteins, cytokines, and various surgical techniques. A recent study reported that transplantation of human umbilical cord blood stem cells (hUCBSCs) achieved therapeutic effects on TON, but the exact effects on Optic Nerve Injury are still unknown, and the mechanisms underlying Nerve protection remain poorly understood. Methods A total of 135 healthy Sprague–Dawley adult rats were randomly assigned to three groups: sham-surgery, model and transplantation, with 45 rats in each group. TON was induced in the model and transplantation groups via Optic Nerve crush Injury. The crush Injury was not performed in the sham-surgery group. Seven days after the Injury, 10 6 hUCBSCs were injected into the rat vitreous cavity of transplantation group, and an equal volume of physiological saline was administered to the model and sham-surgery groups. Pathological observation of rat retina tissues was performed by hematoxylin–eosin (H&E) staining at days 3, 7, 14, 21 and 28 post-surgery. The number of retinal ganglion cells (RGCs) and mRNA expression levels of brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) were assessed by the Fluorogold (FG) retrograde labeling and reverse transcriptase–polymerase chain reaction (RT–PCR) methods, respectively. Results The number of labeled RGCs and the expression of BDNF and GDNF mRNA obviously increased, and pathological Injury was significantly ameliorated in the transplantation group compared to the model group (P<0.05). Conclusions Via intravitreal transplantation, the hUCBSCs resulted in a significant increase in the survival of the RGCs, and improved pathological changes in the rat retina, following TON. The protective mechanism is correlated with the continuous secretion of BDNF and GDNF in vivo of retina in Optic Nerve Injury rats by the transplanted hUCBSCs.

Luosheng Tang - One of the best experts on this subject based on the ideXlab platform.

  • protective effects of human umbilical cord blood stem cell intravitreal transplantation against Optic Nerve Injury in rats
    Graefes Archive for Clinical and Experimental Ophthalmology, 2011
    Co-Authors: Tantai Zhao, Luosheng Tang, Fang Fan, Bing Jiang
    Abstract:

    Background The majority of studies addressing traumatic Optic neuropathy (TON) have focused on drugs, proteins, cytokines, and various surgical techniques. A recent study reported that transplantation of human umbilical cord blood stem cells (hUCBSCs) achieved therapeutic effects on TON, but the exact effects on Optic Nerve Injury are still unknown, and the mechanisms underlying Nerve protection remain poorly understood. Methods A total of 135 healthy Sprague–Dawley adult rats were randomly assigned to three groups: sham-surgery, model and transplantation, with 45 rats in each group. TON was induced in the model and transplantation groups via Optic Nerve crush Injury. The crush Injury was not performed in the sham-surgery group. Seven days after the Injury, 10 6 hUCBSCs were injected into the rat vitreous cavity of transplantation group, and an equal volume of physiological saline was administered to the model and sham-surgery groups. Pathological observation of rat retina tissues was performed by hematoxylin–eosin (H&E) staining at days 3, 7, 14, 21 and 28 post-surgery. The number of retinal ganglion cells (RGCs) and mRNA expression levels of brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) were assessed by the Fluorogold (FG) retrograde labeling and reverse transcriptase–polymerase chain reaction (RT–PCR) methods, respectively. Results The number of labeled RGCs and the expression of BDNF and GDNF mRNA obviously increased, and pathological Injury was significantly ameliorated in the transplantation group compared to the model group (P<0.05). Conclusions Via intravitreal transplantation, the hUCBSCs resulted in a significant increase in the survival of the RGCs, and improved pathological changes in the rat retina, following TON. The protective mechanism is correlated with the continuous secretion of BDNF and GDNF in vivo of retina in Optic Nerve Injury rats by the transplanted hUCBSCs.