Optical Microscope

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The Experts below are selected from a list of 81876 Experts worldwide ranked by ideXlab platform

Yahsin Yang - One of the best experts on this subject based on the ideXlab platform.

Minghung Chiu - One of the best experts on this subject based on the ideXlab platform.

Minghung Tsai - One of the best experts on this subject based on the ideXlab platform.

Chentai Tan - One of the best experts on this subject based on the ideXlab platform.

Satoshi Kawata - One of the best experts on this subject based on the ideXlab platform.

  • near field raman imaging of organic molecules by an apertureless metallic probe scanning Optical Microscope
    Journal of Chemical Physics, 2002
    Co-Authors: Norikiko Hayazawa, Yasushi Inouye, Zouheir Sekkat, Satoshi Kawata
    Abstract:

    Near-field Raman imaging of organic molecules is demonstrated by an apertureless near-field scanning Optical Microscope, the tip of which is a silver-layer-coated cantilever of an atomic force Microscope (AFM). The virtue of the enhanced electric field at the tip apex due to the surface plasmon polariton excitations enhances the Raman scattering cross sections. This phenomenon allows us to reveal from near-field Raman images the molecular vibrational distributions of Rhodamine6G and Crystal Violet molecules beyond the diffraction limit of a light. These molecular vibrations cannot be distinguished by AFM topographic images.

  • evanescent field excitation and measurement of dye fluorescence in a metallic probe near field scanning Optical Microscope
    Journal of Microscopy, 1999
    Co-Authors: Norikiko Hayazawa, Yasushi Inouye, Satoshi Kawata
    Abstract:

    We introduce a method of dye fluorescence excitation and measurement that utilizes a near-field scanning Optical Microscope (NSOM). This NSOM uses an apertureless metallic probe, and an Optical system that contains a high numerical aperture (NA) objective lens (NA = 1.4). When the area which satisfies NA < 1 is masked, the objective lens allows for the rejection of possible transmitted light (NA < 1) through the sample. In such conditions, the focused spot consists of only the evanescent field. We found that this NSOM system strongly reduces the background of the dye fluorescence and allows for the measurement of the fluorescence intensity below the diffraction limit of the excitation source.

  • gold bead scanning near field Optical Microscope with laser force position control
    Optics Letters, 1997
    Co-Authors: Tadao Sugiura, Yasushi Inouye, Takao Okada, Osamu Nakamura, Satoshi Kawata
    Abstract:

    We have developed a scanning near-field Optical Microscope with an Optically trapped metallic particle that has a small diameter compared to the wavelength of visible light. In this Microscope we employed spot illumination to enhance the intensity of light scattered from a probe particle so we could reduce the diameter of the probe particle to 40 nm. We detected slight irregularities of the surface of the cover glass near 10-nm depth. Also, we observed gold colloidal particles on the surface of the cover glass.

  • near field scanning Optical Microscope with a laser trapped probe
    Japanese Journal of Applied Physics, 1994
    Co-Authors: Satoshi Kawata, Yasushi Inouye, Tadao Sugiura
    Abstract:

    We made an experiment of near-field microscopic imaging using a laser-beam trapped probe. Differently from a conventional near-field (and/or photon-tunneling) scanning Optical Microscope, the probe is physically isolated from the scanning Microscope system; it is trapped and scanned on the sample surface by the radiation force of near-infrared laser beam. The distance between the probe and the sample surface is maintained to be constant (zero) during scanning. Another laser beam for microscopic imaging is incident on the sample surface in the condition of total internal reflection; the probe on the sample couples with the photons localized near the sample surface as the evanescent filed and scatters out. The scattered photons are collected through an Microscope objective lens, which is the same lens as the one used for focusing the infrared laser beam on the probe. A near-field image of the sample surface is formed, as the probe is laterally scanned on the sample. The experimental setup of the proposed Microscope is described and the image data obtained with the developed Microscope are shown for refractive samples and fluorescent samples with sub micrometer structure.

  • near field scanning Optical Microscope with a metallic probe tip
    Optics Letters, 1994
    Co-Authors: Yasushi Inouye, Satoshi Kawata
    Abstract:

    A near-field scanning Optical Microscope with a metallic probe tip was developed for detecting localized photons near the surface of the fine structure of a sample. In this Microscope a metallic probe is used for converting the evanescent photons localized near the sample surface to the propagating scattering light wave; the scattered light is detected in the far field with external condenser optics. During the measurement the probe tip vibrates normal to the surface with an amplitude of ~5 nm at 2.5 kHz, and the light intensity modulated with this frequency is lock-in detected. This operation permits the removal of stray-light noise contribution. Experimental results of the measurements of the exponential decay of the evanescent field produced by total internal reflection are given with and without the probe vibration. Image data of the surface profile of an Optical compact disk are also shown.