The Experts below are selected from a list of 16026 Experts worldwide ranked by ideXlab platform

Attila Nagy - One of the best experts on this subject based on the ideXlab platform.

Keir C. Neuman - One of the best experts on this subject based on the ideXlab platform.

Ziqiang Wang - One of the best experts on this subject based on the ideXlab platform.

  • trapping red blood cells in living animals using Optical Tweezers
    Nature Communications, 2013
    Co-Authors: Mincheng Zhong, Jinhua Zhou, Xunbin Wei, Ziqiang Wang
    Abstract:

    Optical Tweezers based on focused laser beams are widely used for biophysical measurements of single molecules in vitro. Here Zhong et al. use infrared Optical Tweezers to trap and manipulate red blood cells within subdermal capillaries in living mice.

  • trapping red blood cells in living animals using Optical Tweezers
    Nature Communications, 2013
    Co-Authors: Mincheng Zhong, Jinhua Zhou, Xunbin Wei, Ziqiang Wang
    Abstract:

    The recent development of non-invasive imaging techniques has enabled the visualization of molecular events underlying cellular processes in live cells. Although microscopic objects can be readily manipulated at the cellular level, additional physiological insight is likely to be gained by manipulation of cells in vivo, which has not been achieved so far. Here we use infrared Optical Tweezers to trap and manipulate red blood cells within subdermal capillaries in living mice. We realize a non-contact micro-operation that results in the clearing of a blocked microvessel. Furthermore, we estimate the Optical trap stiffness in the capillary. Our work expands the application of Optical Tweezers to the study of live cell dynamics in animals.

S Suresh - One of the best experts on this subject based on the ideXlab platform.

  • nonlinear elastic and viscoelastic deformation of the human red blood cell with Optical Tweezers
    Mechanics & chemistry of biosystems : MCB, 2004
    Co-Authors: J P Mills, Lan Qie, Ming Dao, Chwee Teck Lim, S Suresh
    Abstract:

    Studies of the deformation characteristics of single biological cells can offer insights into the connections among mechanical state, biochemical response and the onset and progression of diseases. Deformation imposed by Optical Tweezers provides a useful means for the study of single cell mechanics under a variety of well-controlled stress-states. In this paper, we first critically review recent advances in the study of single cell mechanics employing the Optical Tweezers method, and assess its significance and limitations in comparison to other experimental tools. We then present new experimental and computational results on shape evolution, force-extension curves, elastic properties and viscoelastic response of human red blood cells subjected to large elastic deformation using Optical Tweezers. Potential applications of the methods examined here to study diseased cells are also briefly addressed.

  • mechanics of the human red blood cell deformed by Optical Tweezers
    Journal of The Mechanics and Physics of Solids, 2003
    Co-Authors: S Suresh
    Abstract:

    The mechanical deformation characteristics of living cells are known to influence strongly their chemical and biological functions and the onset, progression and consequences of a number of human diseases. The mechanics of the human red blood cell (erythrocyte) subjected to large deformation by Optical Tweezers forms the subject of this paper. Video photography of the cell deformed in a phosphate buffered saline solution at room temperature during the imposition of controlled stretching forces, in the tens to several hundreds picoNewton range, is used to assess experimentally the deformation characteristics. The mechanical responses of the cell during loading and upon release of the Optical force are then analysed to extract the elastic properties of the cell membrane by recourse to several different constitutive formulations of the elastic and viscoelastic behavior within the framework of a fully three-dimensional finite element analysis. A parametric study of various geometric, loading and structural factors is also undertaken in order to develop quantitative models for the mechanics of deformation by means of Optical Tweezers. The outcome of the experimental and computational analyses is then compared with the information available on the mechanical response of the red blood cell from other independent experimental techniques. Potential applications of the Optical Tweezers method described in this paper to the study of mechanical deformation of living cells under different stress states and in response to the progression of some diseases are also highlighted.

Xunbin Wei - One of the best experts on this subject based on the ideXlab platform.

  • trapping red blood cells in living animals using Optical Tweezers
    Nature Communications, 2013
    Co-Authors: Mincheng Zhong, Jinhua Zhou, Xunbin Wei, Ziqiang Wang
    Abstract:

    Optical Tweezers based on focused laser beams are widely used for biophysical measurements of single molecules in vitro. Here Zhong et al. use infrared Optical Tweezers to trap and manipulate red blood cells within subdermal capillaries in living mice.

  • trapping red blood cells in living animals using Optical Tweezers
    Nature Communications, 2013
    Co-Authors: Mincheng Zhong, Jinhua Zhou, Xunbin Wei, Ziqiang Wang
    Abstract:

    The recent development of non-invasive imaging techniques has enabled the visualization of molecular events underlying cellular processes in live cells. Although microscopic objects can be readily manipulated at the cellular level, additional physiological insight is likely to be gained by manipulation of cells in vivo, which has not been achieved so far. Here we use infrared Optical Tweezers to trap and manipulate red blood cells within subdermal capillaries in living mice. We realize a non-contact micro-operation that results in the clearing of a blocked microvessel. Furthermore, we estimate the Optical trap stiffness in the capillary. Our work expands the application of Optical Tweezers to the study of live cell dynamics in animals.