The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Yanli Mao - One of the best experts on this subject based on the ideXlab platform.
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recyclable soluble insoluble upper critical solution temperature type poly methacrylamide co acrylic acid cellulase biocatalyst for hydrolysis of cellulose into glucose
ACS Sustainable Chemistry & Engineering, 2018Co-Authors: Juan Han, Jing Wan, Yun Wang, Lei Wang, Yanli MaoAbstract:How to improve the accessibility of immobilized cellulase to insoluble cellulose and recover immobilized enzyme from remaining insoluble substrate is a challenge to the efficient hydrolysis of cellulose into glucose. The objective of this work is to solve the problems mentioned above by the immobilization of cellulase onto poly(methacrylamide-co-acrylic acid) (PMAAc), developing a reversibly soluble–insoluble biocatalyst with upper critical solution temperature (UCST) of 16 °C. The as-prepared PMAAc–cellulase with a new UCST of 19 °C exhibited significantly improved pH, temperature, storage, and opeRation stabilities compared with that of free catalyst, and about 82.4% of its original activity was retained even after ten cycles. Cellulase systems containing endo-β-1,4-glucanase (EG), cellobiohydrolase (CBH), and β-glucosidase (β-G) are coimmobilized at an Optimum Ratio on PMAAc by adjusting the additive amount of β-G, which can obtain higher hydrolysis efficiency. It was found that the coimmobilization of...
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Recyclable Soluble–Insoluble Upper Critical Solution Temperature-type Poly(methacrylamide-co-acrylic acid)–Cellulase Biocatalyst for Hydrolysis of Cellulose into Glucose
2018Co-Authors: Juan Han, Jing Wan, Yun Wang, Lei Wang, Yanli MaoAbstract:How to improve the accessibility of immobilized cellulase to insoluble cellulose and recover immobilized enzyme from remaining insoluble substrate is a challenge to the efficient hydrolysis of cellulose into glucose. The objective of this work is to solve the problems mentioned above by the immobilization of cellulase onto poly(methacrylamide-co-acrylic acid) (PMAAc), developing a reversibly soluble–insoluble biocatalyst with upper critical solution temperature (UCST) of 16 °C. The as-prepared PMAAc–cellulase with a new UCST of 19 °C exhibited significantly improved pH, temperature, storage, and opeRation stabilities compared with that of free catalyst, and about 82.4% of its original activity was retained even after ten cycles. Cellulase systems containing endo-β-1,4-glucanase (EG), cellobiohydrolase (CBH), and β-glucosidase (β-G) are coimmobilized at an Optimum Ratio on PMAAc by adjusting the additive amount of β-G, which can obtain higher hydrolysis efficiency. It was found that the coimmobilization of cellulase and β-glucosidase at the Optimum Ratio of 2.5:1 (w/w) showed excellent performance for the hydrolysis of cellulose, and the yield of glucose was up to 89.1% at 50 °C (>UCST) after 24 h, which was 58.4% and 15.4% higher than that of PMAAc–cellulase and free cellulase and β-glucosidase, respectively. The coimmobilized PMAAc–cellulase and β-glucosidase still retained 61.48% of its original productivity after eight cycles of hydrolysis. This novel UCST-type polymer–enzyme catalytic system displays great potential in cellulose biorefining
Juan Han - One of the best experts on this subject based on the ideXlab platform.
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recyclable soluble insoluble upper critical solution temperature type poly methacrylamide co acrylic acid cellulase biocatalyst for hydrolysis of cellulose into glucose
ACS Sustainable Chemistry & Engineering, 2018Co-Authors: Juan Han, Jing Wan, Yun Wang, Lei Wang, Yanli MaoAbstract:How to improve the accessibility of immobilized cellulase to insoluble cellulose and recover immobilized enzyme from remaining insoluble substrate is a challenge to the efficient hydrolysis of cellulose into glucose. The objective of this work is to solve the problems mentioned above by the immobilization of cellulase onto poly(methacrylamide-co-acrylic acid) (PMAAc), developing a reversibly soluble–insoluble biocatalyst with upper critical solution temperature (UCST) of 16 °C. The as-prepared PMAAc–cellulase with a new UCST of 19 °C exhibited significantly improved pH, temperature, storage, and opeRation stabilities compared with that of free catalyst, and about 82.4% of its original activity was retained even after ten cycles. Cellulase systems containing endo-β-1,4-glucanase (EG), cellobiohydrolase (CBH), and β-glucosidase (β-G) are coimmobilized at an Optimum Ratio on PMAAc by adjusting the additive amount of β-G, which can obtain higher hydrolysis efficiency. It was found that the coimmobilization of...
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Recyclable Soluble–Insoluble Upper Critical Solution Temperature-type Poly(methacrylamide-co-acrylic acid)–Cellulase Biocatalyst for Hydrolysis of Cellulose into Glucose
2018Co-Authors: Juan Han, Jing Wan, Yun Wang, Lei Wang, Yanli MaoAbstract:How to improve the accessibility of immobilized cellulase to insoluble cellulose and recover immobilized enzyme from remaining insoluble substrate is a challenge to the efficient hydrolysis of cellulose into glucose. The objective of this work is to solve the problems mentioned above by the immobilization of cellulase onto poly(methacrylamide-co-acrylic acid) (PMAAc), developing a reversibly soluble–insoluble biocatalyst with upper critical solution temperature (UCST) of 16 °C. The as-prepared PMAAc–cellulase with a new UCST of 19 °C exhibited significantly improved pH, temperature, storage, and opeRation stabilities compared with that of free catalyst, and about 82.4% of its original activity was retained even after ten cycles. Cellulase systems containing endo-β-1,4-glucanase (EG), cellobiohydrolase (CBH), and β-glucosidase (β-G) are coimmobilized at an Optimum Ratio on PMAAc by adjusting the additive amount of β-G, which can obtain higher hydrolysis efficiency. It was found that the coimmobilization of cellulase and β-glucosidase at the Optimum Ratio of 2.5:1 (w/w) showed excellent performance for the hydrolysis of cellulose, and the yield of glucose was up to 89.1% at 50 °C (>UCST) after 24 h, which was 58.4% and 15.4% higher than that of PMAAc–cellulase and free cellulase and β-glucosidase, respectively. The coimmobilized PMAAc–cellulase and β-glucosidase still retained 61.48% of its original productivity after eight cycles of hydrolysis. This novel UCST-type polymer–enzyme catalytic system displays great potential in cellulose biorefining
Jing Wan - One of the best experts on this subject based on the ideXlab platform.
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recyclable soluble insoluble upper critical solution temperature type poly methacrylamide co acrylic acid cellulase biocatalyst for hydrolysis of cellulose into glucose
ACS Sustainable Chemistry & Engineering, 2018Co-Authors: Juan Han, Jing Wan, Yun Wang, Lei Wang, Yanli MaoAbstract:How to improve the accessibility of immobilized cellulase to insoluble cellulose and recover immobilized enzyme from remaining insoluble substrate is a challenge to the efficient hydrolysis of cellulose into glucose. The objective of this work is to solve the problems mentioned above by the immobilization of cellulase onto poly(methacrylamide-co-acrylic acid) (PMAAc), developing a reversibly soluble–insoluble biocatalyst with upper critical solution temperature (UCST) of 16 °C. The as-prepared PMAAc–cellulase with a new UCST of 19 °C exhibited significantly improved pH, temperature, storage, and opeRation stabilities compared with that of free catalyst, and about 82.4% of its original activity was retained even after ten cycles. Cellulase systems containing endo-β-1,4-glucanase (EG), cellobiohydrolase (CBH), and β-glucosidase (β-G) are coimmobilized at an Optimum Ratio on PMAAc by adjusting the additive amount of β-G, which can obtain higher hydrolysis efficiency. It was found that the coimmobilization of...
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Recyclable Soluble–Insoluble Upper Critical Solution Temperature-type Poly(methacrylamide-co-acrylic acid)–Cellulase Biocatalyst for Hydrolysis of Cellulose into Glucose
2018Co-Authors: Juan Han, Jing Wan, Yun Wang, Lei Wang, Yanli MaoAbstract:How to improve the accessibility of immobilized cellulase to insoluble cellulose and recover immobilized enzyme from remaining insoluble substrate is a challenge to the efficient hydrolysis of cellulose into glucose. The objective of this work is to solve the problems mentioned above by the immobilization of cellulase onto poly(methacrylamide-co-acrylic acid) (PMAAc), developing a reversibly soluble–insoluble biocatalyst with upper critical solution temperature (UCST) of 16 °C. The as-prepared PMAAc–cellulase with a new UCST of 19 °C exhibited significantly improved pH, temperature, storage, and opeRation stabilities compared with that of free catalyst, and about 82.4% of its original activity was retained even after ten cycles. Cellulase systems containing endo-β-1,4-glucanase (EG), cellobiohydrolase (CBH), and β-glucosidase (β-G) are coimmobilized at an Optimum Ratio on PMAAc by adjusting the additive amount of β-G, which can obtain higher hydrolysis efficiency. It was found that the coimmobilization of cellulase and β-glucosidase at the Optimum Ratio of 2.5:1 (w/w) showed excellent performance for the hydrolysis of cellulose, and the yield of glucose was up to 89.1% at 50 °C (>UCST) after 24 h, which was 58.4% and 15.4% higher than that of PMAAc–cellulase and free cellulase and β-glucosidase, respectively. The coimmobilized PMAAc–cellulase and β-glucosidase still retained 61.48% of its original productivity after eight cycles of hydrolysis. This novel UCST-type polymer–enzyme catalytic system displays great potential in cellulose biorefining
Yun Wang - One of the best experts on this subject based on the ideXlab platform.
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recyclable soluble insoluble upper critical solution temperature type poly methacrylamide co acrylic acid cellulase biocatalyst for hydrolysis of cellulose into glucose
ACS Sustainable Chemistry & Engineering, 2018Co-Authors: Juan Han, Jing Wan, Yun Wang, Lei Wang, Yanli MaoAbstract:How to improve the accessibility of immobilized cellulase to insoluble cellulose and recover immobilized enzyme from remaining insoluble substrate is a challenge to the efficient hydrolysis of cellulose into glucose. The objective of this work is to solve the problems mentioned above by the immobilization of cellulase onto poly(methacrylamide-co-acrylic acid) (PMAAc), developing a reversibly soluble–insoluble biocatalyst with upper critical solution temperature (UCST) of 16 °C. The as-prepared PMAAc–cellulase with a new UCST of 19 °C exhibited significantly improved pH, temperature, storage, and opeRation stabilities compared with that of free catalyst, and about 82.4% of its original activity was retained even after ten cycles. Cellulase systems containing endo-β-1,4-glucanase (EG), cellobiohydrolase (CBH), and β-glucosidase (β-G) are coimmobilized at an Optimum Ratio on PMAAc by adjusting the additive amount of β-G, which can obtain higher hydrolysis efficiency. It was found that the coimmobilization of...
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Recyclable Soluble–Insoluble Upper Critical Solution Temperature-type Poly(methacrylamide-co-acrylic acid)–Cellulase Biocatalyst for Hydrolysis of Cellulose into Glucose
2018Co-Authors: Juan Han, Jing Wan, Yun Wang, Lei Wang, Yanli MaoAbstract:How to improve the accessibility of immobilized cellulase to insoluble cellulose and recover immobilized enzyme from remaining insoluble substrate is a challenge to the efficient hydrolysis of cellulose into glucose. The objective of this work is to solve the problems mentioned above by the immobilization of cellulase onto poly(methacrylamide-co-acrylic acid) (PMAAc), developing a reversibly soluble–insoluble biocatalyst with upper critical solution temperature (UCST) of 16 °C. The as-prepared PMAAc–cellulase with a new UCST of 19 °C exhibited significantly improved pH, temperature, storage, and opeRation stabilities compared with that of free catalyst, and about 82.4% of its original activity was retained even after ten cycles. Cellulase systems containing endo-β-1,4-glucanase (EG), cellobiohydrolase (CBH), and β-glucosidase (β-G) are coimmobilized at an Optimum Ratio on PMAAc by adjusting the additive amount of β-G, which can obtain higher hydrolysis efficiency. It was found that the coimmobilization of cellulase and β-glucosidase at the Optimum Ratio of 2.5:1 (w/w) showed excellent performance for the hydrolysis of cellulose, and the yield of glucose was up to 89.1% at 50 °C (>UCST) after 24 h, which was 58.4% and 15.4% higher than that of PMAAc–cellulase and free cellulase and β-glucosidase, respectively. The coimmobilized PMAAc–cellulase and β-glucosidase still retained 61.48% of its original productivity after eight cycles of hydrolysis. This novel UCST-type polymer–enzyme catalytic system displays great potential in cellulose biorefining
Lei Wang - One of the best experts on this subject based on the ideXlab platform.
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recyclable soluble insoluble upper critical solution temperature type poly methacrylamide co acrylic acid cellulase biocatalyst for hydrolysis of cellulose into glucose
ACS Sustainable Chemistry & Engineering, 2018Co-Authors: Juan Han, Jing Wan, Yun Wang, Lei Wang, Yanli MaoAbstract:How to improve the accessibility of immobilized cellulase to insoluble cellulose and recover immobilized enzyme from remaining insoluble substrate is a challenge to the efficient hydrolysis of cellulose into glucose. The objective of this work is to solve the problems mentioned above by the immobilization of cellulase onto poly(methacrylamide-co-acrylic acid) (PMAAc), developing a reversibly soluble–insoluble biocatalyst with upper critical solution temperature (UCST) of 16 °C. The as-prepared PMAAc–cellulase with a new UCST of 19 °C exhibited significantly improved pH, temperature, storage, and opeRation stabilities compared with that of free catalyst, and about 82.4% of its original activity was retained even after ten cycles. Cellulase systems containing endo-β-1,4-glucanase (EG), cellobiohydrolase (CBH), and β-glucosidase (β-G) are coimmobilized at an Optimum Ratio on PMAAc by adjusting the additive amount of β-G, which can obtain higher hydrolysis efficiency. It was found that the coimmobilization of...
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Recyclable Soluble–Insoluble Upper Critical Solution Temperature-type Poly(methacrylamide-co-acrylic acid)–Cellulase Biocatalyst for Hydrolysis of Cellulose into Glucose
2018Co-Authors: Juan Han, Jing Wan, Yun Wang, Lei Wang, Yanli MaoAbstract:How to improve the accessibility of immobilized cellulase to insoluble cellulose and recover immobilized enzyme from remaining insoluble substrate is a challenge to the efficient hydrolysis of cellulose into glucose. The objective of this work is to solve the problems mentioned above by the immobilization of cellulase onto poly(methacrylamide-co-acrylic acid) (PMAAc), developing a reversibly soluble–insoluble biocatalyst with upper critical solution temperature (UCST) of 16 °C. The as-prepared PMAAc–cellulase with a new UCST of 19 °C exhibited significantly improved pH, temperature, storage, and opeRation stabilities compared with that of free catalyst, and about 82.4% of its original activity was retained even after ten cycles. Cellulase systems containing endo-β-1,4-glucanase (EG), cellobiohydrolase (CBH), and β-glucosidase (β-G) are coimmobilized at an Optimum Ratio on PMAAc by adjusting the additive amount of β-G, which can obtain higher hydrolysis efficiency. It was found that the coimmobilization of cellulase and β-glucosidase at the Optimum Ratio of 2.5:1 (w/w) showed excellent performance for the hydrolysis of cellulose, and the yield of glucose was up to 89.1% at 50 °C (>UCST) after 24 h, which was 58.4% and 15.4% higher than that of PMAAc–cellulase and free cellulase and β-glucosidase, respectively. The coimmobilized PMAAc–cellulase and β-glucosidase still retained 61.48% of its original productivity after eight cycles of hydrolysis. This novel UCST-type polymer–enzyme catalytic system displays great potential in cellulose biorefining