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Dewa Gede Semara Edi - One of the best experts on this subject based on the ideXlab platform.

  • Analysis of Microbial Contamination in Oreochromis niloticus in Waduk Muara Nusa Dua
    SEAS (Sustainable Environment Agricultural Science), 2020
    Co-Authors: Ni Kadek Peniasih, I Gede Suranaya Pandit, Dewa Gede Semara Edi
    Abstract:

    This study aims to determine the microbiological contamination in the main water source of the Muara Nusa Dua Reservoir, the Tukad Badung River, and to determine the microbiological quality of fresh Oreochromis niloticus caught in the Muara Nusa Dua Reservoir in Denpasar. The study was conducted in May-August 2019 at the Denpasar Veterinary Laboratory. The method used is a descriptive method that aims to describe or describe the condition of the Tukad Badung watershed which empties into the Muara Nusa Dua Reservoir (Estuary Dam). Objective tests include the total bacterial test, the Coliform test, and the Escherichia coli contamination test. Water quality measurements are also carried out as supporting data from this study. The results of the study stated that there was microbiological contamination in the water source of the Muara Nusa Dua Reservoir, the Tukad Badung river, which also had an impact on the waters of the Muara Nusa Dua Reservoir. Microbiological contamination research results showed the microbiological quality of ten samples of Oreochromis niloticus in the form of the highest total bacteria was at 2.2x104, the highest total Coliform was 1.9x104 and the highest Escherichia coli contamination was at 93 APM / g with an average reservoir water quality Muara Nusa Dua in the form of temperature 27.3 °C, salinity 0.208 ‰, degree of acidity (pH) 6.5, dissolved oxygen (DO) 5,97 ppm. Organoleptic observations included the appearance, smell, and texture of Oreochromis niloticus with good results because when testing Oreochromis niloticus is still alive

  • Analysis of Microbial Contamination in Oreochromis niloticus in Waduk Muara Nusa Dua
    'Universitas Warmadewa', 2020
    Co-Authors: Ni Kadek Peniasih, Gede Suranaya I Pandit, Dewa Gede Semara Edi
    Abstract:

    This study aims to determine the microbiological contamination in the main water source of the Muara Nusa Dua Reservoir, the Tukad Badung River, and to determine the microbiological quality of fresh Oreochromis niloticus caught in the Muara Nusa Dua Reservoir in Denpasar. The study was conducted in May-August 2019 at the Denpasar Veterinary Laboratory. The method used is a descriptive method that aims to describe or describe the condition of the Tukad Badung watershed which empties into the Muara Nusa Dua Reservoir (Estuary Dam). Objective tests include the total bacterial test, the Coliform test, and the Escherichia coli contamination test. Water quality measurements are also carried out as supporting data from this study. The results of the study stated that there was microbiological contamination in the water source of the Muara Nusa Dua Reservoir, the Tukad Badung river, which also had an impact on the waters of the Muara Nusa Dua Reservoir. Microbiological contamination research results showed the microbiological quality of ten samples of Oreochromis niloticus in the form of the highest total bacteria was at 2.2x104, the highest total Coliform was 1.9x104 and the highest Escherichia coli contamination was at 93 APM / g with an average reservoir water quality Muara Nusa Dua in the form of temperature 27.3 °C, salinity 0.208 ‰, degree of acidity (pH) 6.5, dissolved oxygen (DO) 5,97 ppm. Organoleptic observations included the appearance, smell, and texture of Oreochromis niloticus with good results because when testing Oreochromis niloticus is still aliv

Ni Kadek Peniasih - One of the best experts on this subject based on the ideXlab platform.

  • Analysis of Microbial Contamination in Oreochromis niloticus in Waduk Muara Nusa Dua
    SEAS (Sustainable Environment Agricultural Science), 2020
    Co-Authors: Ni Kadek Peniasih, I Gede Suranaya Pandit, Dewa Gede Semara Edi
    Abstract:

    This study aims to determine the microbiological contamination in the main water source of the Muara Nusa Dua Reservoir, the Tukad Badung River, and to determine the microbiological quality of fresh Oreochromis niloticus caught in the Muara Nusa Dua Reservoir in Denpasar. The study was conducted in May-August 2019 at the Denpasar Veterinary Laboratory. The method used is a descriptive method that aims to describe or describe the condition of the Tukad Badung watershed which empties into the Muara Nusa Dua Reservoir (Estuary Dam). Objective tests include the total bacterial test, the Coliform test, and the Escherichia coli contamination test. Water quality measurements are also carried out as supporting data from this study. The results of the study stated that there was microbiological contamination in the water source of the Muara Nusa Dua Reservoir, the Tukad Badung river, which also had an impact on the waters of the Muara Nusa Dua Reservoir. Microbiological contamination research results showed the microbiological quality of ten samples of Oreochromis niloticus in the form of the highest total bacteria was at 2.2x104, the highest total Coliform was 1.9x104 and the highest Escherichia coli contamination was at 93 APM / g with an average reservoir water quality Muara Nusa Dua in the form of temperature 27.3 °C, salinity 0.208 ‰, degree of acidity (pH) 6.5, dissolved oxygen (DO) 5,97 ppm. Organoleptic observations included the appearance, smell, and texture of Oreochromis niloticus with good results because when testing Oreochromis niloticus is still alive

  • Analysis of Microbial Contamination in Oreochromis niloticus in Waduk Muara Nusa Dua
    'Universitas Warmadewa', 2020
    Co-Authors: Ni Kadek Peniasih, Gede Suranaya I Pandit, Dewa Gede Semara Edi
    Abstract:

    This study aims to determine the microbiological contamination in the main water source of the Muara Nusa Dua Reservoir, the Tukad Badung River, and to determine the microbiological quality of fresh Oreochromis niloticus caught in the Muara Nusa Dua Reservoir in Denpasar. The study was conducted in May-August 2019 at the Denpasar Veterinary Laboratory. The method used is a descriptive method that aims to describe or describe the condition of the Tukad Badung watershed which empties into the Muara Nusa Dua Reservoir (Estuary Dam). Objective tests include the total bacterial test, the Coliform test, and the Escherichia coli contamination test. Water quality measurements are also carried out as supporting data from this study. The results of the study stated that there was microbiological contamination in the water source of the Muara Nusa Dua Reservoir, the Tukad Badung river, which also had an impact on the waters of the Muara Nusa Dua Reservoir. Microbiological contamination research results showed the microbiological quality of ten samples of Oreochromis niloticus in the form of the highest total bacteria was at 2.2x104, the highest total Coliform was 1.9x104 and the highest Escherichia coli contamination was at 93 APM / g with an average reservoir water quality Muara Nusa Dua in the form of temperature 27.3 °C, salinity 0.208 ‰, degree of acidity (pH) 6.5, dissolved oxygen (DO) 5,97 ppm. Organoleptic observations included the appearance, smell, and texture of Oreochromis niloticus with good results because when testing Oreochromis niloticus is still aliv

Rodrigo Fortes-silva - One of the best experts on this subject based on the ideXlab platform.

Alkhateib Y. Gaafar - One of the best experts on this subject based on the ideXlab platform.

  • Immunosuppressive effect of cyclophosphamide in Nile tilapia (Oreochromis niloticus)
    Environmental Science and Pollution Research, 2021
    Co-Authors: Mohamed F. A. Elazab, Abdelgayed M. Younes, Alkhateib Y. Gaafar, Alaa Z. Abu-bryka, Mohamed M. Abdel-daim
    Abstract:

    Cyclophosphamide, a common chemotherapeutic and immune suppressor agent, is regularly used in research to weaken the immune system in laboratory animal models. Nile tilapia ( Oreochromis niloticus ) is a widely used experimental fish model for immune-modulatory research; with the lack of knowledge about the immune-compromised tilapia model, an urgent need is to develop and optimise such a model. Sixty healthy Oreochromis niloticus fish, average weight 50 ± 10 g, were divided into four experimental groups. Fish in group I, negative control group, were injected with phosphate-buffered saline only, and fish in groups II, III and IV were injected with cyclophosphamide (CP) at 100, 200 and 400 mg/kg body weight (BW), respectively, via the intraperitoneal route. Different immune-related parameters were investigated 3 weeks after CP injection. The results have revealed a significant decrease in total red blood cells (RBCs), white blood cells (WBCs) and thrombocyte counts and reduced haemoglobin and haematocrit values in CP-treated fish, especially those injected with 200 mg/kg BW compared with the control group ( p < 0.05). Also, significantly lower levels of serum proteins (total protein, albumin, α1- and γ-globulins) were observed in CP-treated fish, especially those injected with 200 mg/kg BW in comparison with the control group ( p < 0.05). Furthermore, CP-treated fish showed a reduction in the expression of immune-related genes (interleukin-1, and tumour necrosis factor-α in spleen and tumour necrosis factor-α and transforming growth factor β-1 in head-kidney), especially those injected with 200 mg/kg BW compared with the control group ( p < 0.05). In conclusion, the Oreochromis niloticus immune-suppressed model can be induced by intraperitoneal CP injection at 200 mg/kg BW.

  • Characterization and Pathogen Challenge of Pseudomonas Species from Oreochromis niloticus in Egypt.
    2015
    Co-Authors: Abdelgayed M. Younes, Laila A. Mohamed, Alkhateib Y. Gaafar
    Abstract:

    Fish diseases caused by Pseudomonas species are responsible for considerable economic losses in the commercial aquaculture of Oreochromis niloticus in Egypt. The present study was undertaken in order to draw up the characterization of pseudomonas species isolated from different localities in Egypt. The prevalence of Pseudomonas spp. isolated were 30 % from naturally infected Oreochromis niloticus, with highest prevalence of P. fluorescence (60%). All isolates were characterized by proteolytic, lipolytic activities and lecithinases production. Using16S-23S PCR ribotyping, the P. fluorescens isolates were divided for 4 biotypes. Fish challenge results showed that all P. fluorescens were pathogenic, while the P. anguilliseptica isolates were

Bartira Guerra-santos - One of the best experts on this subject based on the ideXlab platform.