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Kyoko Hotta - One of the best experts on this subject based on the ideXlab platform.

  • stimulant effect of nitric oxide generator and roxatidine on mucin biosynthesis of rat gastric oxyntic mucosa
    Life Sciences, 1999
    Co-Authors: Takafumi Ichikawa, Kazuhiko Ishihara, Tatsumi Kusakabe, Tadashi Kawakami, Kyoko Hotta
    Abstract:

    Although the involvement of nitric oxide(NO) in an increasing gastric mucus metabolism has been reported, information on whether or not its activation is limited to the specific mucus-producing cells is lacking. In this paper, we report the effect of the exogenous NO-donor, isosorbide dinitrate(ISDN), and second-generation histamine H2 receptor antagonist roxatidine (2-acetoxy-N-(3-[m-(1-piperidinylmethyl)phenoxy]propyl)acetamide hydrochloride) which is demonstrated to accelerate the mucin metabolism mediated by endogenous NO, on the mucin biosynthesis in distinct sites and layers of the rat gastric mucosa using an Organ Culture Technique. Radiolabeled mucin was obtained from the tissue of full-thickness and the deep corpus layer, and the antrum of the rat stomach incubated for 5 hr with [3H]glucosamine(GlcN) in vitro. With the addition of ISDN to the Culture medium, 3H-labeled mucin in the full-thickness corpus mucosa increased to 124–145% of the control (p < 0.05), but not in the antrum. This stimulation of the mucin synthesis disappeared by the removal treatment of the surface mucous cell layer which has immunoreactivity of neuronal NO synthase. Similarly, roxatidine stimulated the mucin biosynthesis in the full-thickness corpus mucosa, but not in the gland mucous cell layer. These results suggest that the stimulation of the mucin biosynthesis mediated by NO is restricted to the surface mucous cells of the rat gastric oxyntic mucosa.

  • effects of acid inhibitory antiulcer drugs on mucin biosynthesis in the rat stomach
    European Journal of Pharmacology, 1994
    Co-Authors: Takafumi Ichikawa, Kazuhiko Ishihara, Katsunori Saigenji, Kyoko Hotta
    Abstract:

    Abstract The effects of the anti-acid secretory agents, cimetidine (ifN-cyano-N′-methyl-N″-([5-methyl-1H-imidazol-4-yl)methyl]thio)ethyl)guanidine), ranitidini (N-(2-(((-5[(dimethylamino)methyl]-2-furanyl)methyl)thio)ethyl)-N′-methyl-2-nitro-1,1-ethe-diamine), roxatidini (2-acetoxy-N-(3-[m-(1-piperidinylmethyl)phenoxyl]-propyl)acetamide hydrochloride), FRG-8813 (2-(furfurylsulfinyl)-N-(4-[4-(piperidinomethyl)-2-pyridyl]oxy-(z)-2-butenyl)acetanide), omeprazole (5-methoxy-2-([(4-methoxy-3,5-dimethylpyridinyl)methyl]sulfinyl)-1H-benzimidazole and NC-1300-O-3 (2-([2-(isobutylmethylamino)benzyl]sulfinyl)-1H-benzimidazole), on mucin biosynthesis were studied in rat gastric mucosa by using an Organ Culture Technique. [3H]Glucosamine incorporation was stimulated in the corpus region by the histamine H2 receptor antagonists which have a six-membered aromatic ring, roxatidine and FRG-8813, and the new H+, K+-ATPase inhibitor, NC-1300-O-3. Thus, these drugs not only inhibit acid secretion but may also promote gastric mucosal protective actions. The present observations also demonstrate that the determination of mucin biosynthesis may be a useful tool for evaluation of mucosal protective activity.

Takafumi Ichikawa - One of the best experts on this subject based on the ideXlab platform.

  • stimulant effect of nitric oxide generator and roxatidine on mucin biosynthesis of rat gastric oxyntic mucosa
    Life Sciences, 1999
    Co-Authors: Takafumi Ichikawa, Kazuhiko Ishihara, Tatsumi Kusakabe, Tadashi Kawakami, Kyoko Hotta
    Abstract:

    Although the involvement of nitric oxide(NO) in an increasing gastric mucus metabolism has been reported, information on whether or not its activation is limited to the specific mucus-producing cells is lacking. In this paper, we report the effect of the exogenous NO-donor, isosorbide dinitrate(ISDN), and second-generation histamine H2 receptor antagonist roxatidine (2-acetoxy-N-(3-[m-(1-piperidinylmethyl)phenoxy]propyl)acetamide hydrochloride) which is demonstrated to accelerate the mucin metabolism mediated by endogenous NO, on the mucin biosynthesis in distinct sites and layers of the rat gastric mucosa using an Organ Culture Technique. Radiolabeled mucin was obtained from the tissue of full-thickness and the deep corpus layer, and the antrum of the rat stomach incubated for 5 hr with [3H]glucosamine(GlcN) in vitro. With the addition of ISDN to the Culture medium, 3H-labeled mucin in the full-thickness corpus mucosa increased to 124–145% of the control (p < 0.05), but not in the antrum. This stimulation of the mucin synthesis disappeared by the removal treatment of the surface mucous cell layer which has immunoreactivity of neuronal NO synthase. Similarly, roxatidine stimulated the mucin biosynthesis in the full-thickness corpus mucosa, but not in the gland mucous cell layer. These results suggest that the stimulation of the mucin biosynthesis mediated by NO is restricted to the surface mucous cells of the rat gastric oxyntic mucosa.

  • effects of acid inhibitory antiulcer drugs on mucin biosynthesis in the rat stomach
    European Journal of Pharmacology, 1994
    Co-Authors: Takafumi Ichikawa, Kazuhiko Ishihara, Katsunori Saigenji, Kyoko Hotta
    Abstract:

    Abstract The effects of the anti-acid secretory agents, cimetidine (ifN-cyano-N′-methyl-N″-([5-methyl-1H-imidazol-4-yl)methyl]thio)ethyl)guanidine), ranitidini (N-(2-(((-5[(dimethylamino)methyl]-2-furanyl)methyl)thio)ethyl)-N′-methyl-2-nitro-1,1-ethe-diamine), roxatidini (2-acetoxy-N-(3-[m-(1-piperidinylmethyl)phenoxyl]-propyl)acetamide hydrochloride), FRG-8813 (2-(furfurylsulfinyl)-N-(4-[4-(piperidinomethyl)-2-pyridyl]oxy-(z)-2-butenyl)acetanide), omeprazole (5-methoxy-2-([(4-methoxy-3,5-dimethylpyridinyl)methyl]sulfinyl)-1H-benzimidazole and NC-1300-O-3 (2-([2-(isobutylmethylamino)benzyl]sulfinyl)-1H-benzimidazole), on mucin biosynthesis were studied in rat gastric mucosa by using an Organ Culture Technique. [3H]Glucosamine incorporation was stimulated in the corpus region by the histamine H2 receptor antagonists which have a six-membered aromatic ring, roxatidine and FRG-8813, and the new H+, K+-ATPase inhibitor, NC-1300-O-3. Thus, these drugs not only inhibit acid secretion but may also promote gastric mucosal protective actions. The present observations also demonstrate that the determination of mucin biosynthesis may be a useful tool for evaluation of mucosal protective activity.

Takehiko Ogawa - One of the best experts on this subject based on the ideXlab platform.

  • In vitro spermatogenesis using an Organ Culture Technique.
    Methods in molecular biology (Clifton N.J.), 2012
    Co-Authors: Tetsuhiro Yokonishi, Takuya Sato, Kumiko Katagiri, Takehiko Ogawa
    Abstract:

    Research on in vitro spermatogenesis has a long history and remained to be an unaccomplished task until very recently. In 2010, we succeeded in producing murine sperm from primitive spermatogonia using an Organ Culture method. The fertility of the sperm or haploid spermatids was demonstrated by microinsemination. This Organ Culture Technique uses the classical air-liquid interphase method and is based on conditions extensively examined by Steinbergers in 1960s. Among adaptations in the new Culture system, application of serum-free media was the most important. The system is very simple and easy to follow.

Kazuhiko Ishihara - One of the best experts on this subject based on the ideXlab platform.

  • stimulant effect of nitric oxide generator and roxatidine on mucin biosynthesis of rat gastric oxyntic mucosa
    Life Sciences, 1999
    Co-Authors: Takafumi Ichikawa, Kazuhiko Ishihara, Tatsumi Kusakabe, Tadashi Kawakami, Kyoko Hotta
    Abstract:

    Although the involvement of nitric oxide(NO) in an increasing gastric mucus metabolism has been reported, information on whether or not its activation is limited to the specific mucus-producing cells is lacking. In this paper, we report the effect of the exogenous NO-donor, isosorbide dinitrate(ISDN), and second-generation histamine H2 receptor antagonist roxatidine (2-acetoxy-N-(3-[m-(1-piperidinylmethyl)phenoxy]propyl)acetamide hydrochloride) which is demonstrated to accelerate the mucin metabolism mediated by endogenous NO, on the mucin biosynthesis in distinct sites and layers of the rat gastric mucosa using an Organ Culture Technique. Radiolabeled mucin was obtained from the tissue of full-thickness and the deep corpus layer, and the antrum of the rat stomach incubated for 5 hr with [3H]glucosamine(GlcN) in vitro. With the addition of ISDN to the Culture medium, 3H-labeled mucin in the full-thickness corpus mucosa increased to 124–145% of the control (p < 0.05), but not in the antrum. This stimulation of the mucin synthesis disappeared by the removal treatment of the surface mucous cell layer which has immunoreactivity of neuronal NO synthase. Similarly, roxatidine stimulated the mucin biosynthesis in the full-thickness corpus mucosa, but not in the gland mucous cell layer. These results suggest that the stimulation of the mucin biosynthesis mediated by NO is restricted to the surface mucous cells of the rat gastric oxyntic mucosa.

  • effects of acid inhibitory antiulcer drugs on mucin biosynthesis in the rat stomach
    European Journal of Pharmacology, 1994
    Co-Authors: Takafumi Ichikawa, Kazuhiko Ishihara, Katsunori Saigenji, Kyoko Hotta
    Abstract:

    Abstract The effects of the anti-acid secretory agents, cimetidine (ifN-cyano-N′-methyl-N″-([5-methyl-1H-imidazol-4-yl)methyl]thio)ethyl)guanidine), ranitidini (N-(2-(((-5[(dimethylamino)methyl]-2-furanyl)methyl)thio)ethyl)-N′-methyl-2-nitro-1,1-ethe-diamine), roxatidini (2-acetoxy-N-(3-[m-(1-piperidinylmethyl)phenoxyl]-propyl)acetamide hydrochloride), FRG-8813 (2-(furfurylsulfinyl)-N-(4-[4-(piperidinomethyl)-2-pyridyl]oxy-(z)-2-butenyl)acetanide), omeprazole (5-methoxy-2-([(4-methoxy-3,5-dimethylpyridinyl)methyl]sulfinyl)-1H-benzimidazole and NC-1300-O-3 (2-([2-(isobutylmethylamino)benzyl]sulfinyl)-1H-benzimidazole), on mucin biosynthesis were studied in rat gastric mucosa by using an Organ Culture Technique. [3H]Glucosamine incorporation was stimulated in the corpus region by the histamine H2 receptor antagonists which have a six-membered aromatic ring, roxatidine and FRG-8813, and the new H+, K+-ATPase inhibitor, NC-1300-O-3. Thus, these drugs not only inhibit acid secretion but may also promote gastric mucosal protective actions. The present observations also demonstrate that the determination of mucin biosynthesis may be a useful tool for evaluation of mucosal protective activity.

John C Marshall - One of the best experts on this subject based on the ideXlab platform.

  • cellular effects of mitomycin c on human corneas after photorefractive keratectomy
    Journal of Cataract and Refractive Surgery, 2006
    Co-Authors: Madhavan S Rajan, David P S Obrart, A Patmore, John C Marshall
    Abstract:

    Purpose To investigate the effects of mitomycin-C (MMC) on epithelial and keratocyte cell kinetics after photorefractive keratectomy (PRK) using an in vitro human cornea model. Setting Department of Academic Ophthalmology, Rayne Institute, St. Thomas' Hospital, London, United Kingdom. Methods Twenty-four human eye-bank corneas were placed in a specially designed acrylic corneal holder and Cultured using the air-interface Organ Culture Technique for up to 4 weeks. The corneas were divided into 3 groups. Group 1 consisted of 8 human corneas that had −9.00 diopter (D) myopic PRK without MMC application. Group 2 consisted of 8 corneas that had −9.00 D PRK with MMC (0.2 μg/mL) application for 1 minute on the stromal surface after ablation. Group 3 consisted of 8 corneas that had −9.00 D PRK with 2-minute exposure to MMC (0.2 μg/mL). Temporal events in epithelial and keratocyte cell kinetics were evaluated using digital imaging, confocal microscopy, and light microscopy. Results Epithelial latency was significantly delayed with MMC application in Groups 2 and 3 ( P P P P P P Conclusions Results suggest that epithelial healing after MMC is characterized by prolonged latency and decreased migration rate dependent on exposure time. Mitomycin C application did not result in increased loss of keratocytes, but it significantly delayed keratocyte repopulation in the anterior stroma. The use of MMC 0.2 μg/mL for 1 minute resulted in optimum modulation of healing characterized by reduced keratocyte activation with normal epithelial differentiation.