The Experts below are selected from a list of 183 Experts worldwide ranked by ideXlab platform

Tomasz Banasiewicz - One of the best experts on this subject based on the ideXlab platform.

Tim W. O'c Maskell - One of the best experts on this subject based on the ideXlab platform.

  • Phage as a diagnostic - The use of phage in TB diagnosis
    Journal of Chemical Technology and Biotechnology, 2001
    Co-Authors: Richard J. Mole, Tim W. O'c Maskell
    Abstract:

    Rapid TB diagnosis and drug susceptibility testing is limited by the slow growth of the causative organism, Mycobacterium tuberculosis (MTB). Effective and rapid methods for the detection and drug susceptibility testing of TB, that are suitable for implementation in low income countries, which bear the highest TB burden, are urgently needed for effective identification, treatment and control of the disease. Mycobacteriophages have the potential to become useful tools in the diagnosis of TB, as they are specific for mycobacteria and only replicate in, and hence detect, viable cells. Phage-based techniques involve simple manual manipulations that typically require little capital expense and yield results rapidly. Two techniques have been developed recently which use mycobacteriophage for the antibiotic susceptibility testing and direct diagnosis of TB from sputum. Luciferase Reporter Phages (LRP) are recombinant phages that contain the gene for the fire-fly luciferase enzyme. Upon infection of mycobacteria by these phages, this enzyme is produced within a cell. In the presence of this enzyme, light is produced by viable cells that also contain the substrates for the enzyme: ATP and luciferin (added exogenously). The 'Bronx Box' has been developed as a low cost means of detecting light signal from mycobacteria infected with LRP using photographic film. A MTB strain's susceptibility to an antibiotic can be assessed by comparing light production, as an indication of the presence of viable cells, in samples pre-incubated in the presence and absence of the test antibiotic. Susceptibility testing of some of the front line drugs for TB chemotherapy has given excellent results (detection of all resistant isolates), using LRP and the Bronx Box. Phage amplification uses the production of progeny phage, instead of a reporter gene product, to reflect the presence of target bacteria. The technique has been successfully applied to drug susceptibility testing and for diagnosis to the TB from sputum Specimens. Viable MTB cells within a Specimen are uniquely infected by a bacteriophage reagent. A potent selective virucide destroys all the phage particles that remain outside the cells while remaining inactive against the MTB bacilli (and the phages that have infected them). After the virucide has been neutralised, and phages that are released from infected cells are indicative of TB cells being present in the Original Specimen. These phages can be easily detected by propagation on lawns of non-pathogenic rapidly growing mycobacteria. A commercially available test, termed FASTPlaqueTB-RIF, determines the rifampicin susceptibility of isolates by comparing the viability of cells pre-treated with and without refampicin. Results give excellent agreement (97-98% overall) with conventional 'gold standard' methods, but in 2 days rather than 3 weeks. Preliminary work has shown that it should be possible to reduce the assay time to 24h. A variation to this method, FASTPlaqueTB, detects TB directly from sputum Specimens and is capable of rapidly reporting the presence of viable MTB cells within 48 h. This technique has achieved excellent specificity (close to 100%) when evaluated in the clinical setting. 2001 Society of Chemical Industry.

Adam Bobkiewicz - One of the best experts on this subject based on the ideXlab platform.

Richard J. Mole - One of the best experts on this subject based on the ideXlab platform.

  • Phage as a diagnostic - The use of phage in TB diagnosis
    Journal of Chemical Technology and Biotechnology, 2001
    Co-Authors: Richard J. Mole, Tim W. O'c Maskell
    Abstract:

    Rapid TB diagnosis and drug susceptibility testing is limited by the slow growth of the causative organism, Mycobacterium tuberculosis (MTB). Effective and rapid methods for the detection and drug susceptibility testing of TB, that are suitable for implementation in low income countries, which bear the highest TB burden, are urgently needed for effective identification, treatment and control of the disease. Mycobacteriophages have the potential to become useful tools in the diagnosis of TB, as they are specific for mycobacteria and only replicate in, and hence detect, viable cells. Phage-based techniques involve simple manual manipulations that typically require little capital expense and yield results rapidly. Two techniques have been developed recently which use mycobacteriophage for the antibiotic susceptibility testing and direct diagnosis of TB from sputum. Luciferase Reporter Phages (LRP) are recombinant phages that contain the gene for the fire-fly luciferase enzyme. Upon infection of mycobacteria by these phages, this enzyme is produced within a cell. In the presence of this enzyme, light is produced by viable cells that also contain the substrates for the enzyme: ATP and luciferin (added exogenously). The 'Bronx Box' has been developed as a low cost means of detecting light signal from mycobacteria infected with LRP using photographic film. A MTB strain's susceptibility to an antibiotic can be assessed by comparing light production, as an indication of the presence of viable cells, in samples pre-incubated in the presence and absence of the test antibiotic. Susceptibility testing of some of the front line drugs for TB chemotherapy has given excellent results (detection of all resistant isolates), using LRP and the Bronx Box. Phage amplification uses the production of progeny phage, instead of a reporter gene product, to reflect the presence of target bacteria. The technique has been successfully applied to drug susceptibility testing and for diagnosis to the TB from sputum Specimens. Viable MTB cells within a Specimen are uniquely infected by a bacteriophage reagent. A potent selective virucide destroys all the phage particles that remain outside the cells while remaining inactive against the MTB bacilli (and the phages that have infected them). After the virucide has been neutralised, and phages that are released from infected cells are indicative of TB cells being present in the Original Specimen. These phages can be easily detected by propagation on lawns of non-pathogenic rapidly growing mycobacteria. A commercially available test, termed FASTPlaqueTB-RIF, determines the rifampicin susceptibility of isolates by comparing the viability of cells pre-treated with and without refampicin. Results give excellent agreement (97-98% overall) with conventional 'gold standard' methods, but in 2 days rather than 3 weeks. Preliminary work has shown that it should be possible to reduce the assay time to 24h. A variation to this method, FASTPlaqueTB, detects TB directly from sputum Specimens and is capable of rapidly reporting the presence of viable MTB cells within 48 h. This technique has achieved excellent specificity (close to 100%) when evaluated in the clinical setting. 2001 Society of Chemical Industry.

Bin Li - One of the best experts on this subject based on the ideXlab platform.

  • microstructure and mechanical properties from an attractive combination of plasma nitriding and secondary hardening of m50 steel
    Applied Surface Science, 2018
    Co-Authors: Baofeng Chen, Bin Li
    Abstract:

    Abstract Microstructure and mechanical properties of a quenched M50 steel plasma nitrided for 8 h at temperatures ranging from 480 to 540 °C in a gas mixture of 25% N2 + 75% H2 were investigated in this paper. Results show that, compared with the Original Specimen, microhardness and wear resistance of M50 steel after nitriding treatment are remarkably improved at various temperatures, wherein the N500 Specimen achieved the optimum combination of surface hardness (1186 HV), substrate hardness (643HV) and wear resistance (wear rate reduced by 90% more than Original Specimen). Such significantly improvement could mainly be attributed to the formation of nanophase FeN0.076, occurrence of further secondary hardening of substrate and generation of a nitrided surface layer of M50 steel during nitriding process. Two types of carbides, identified as MC and M6C, were found to be the stable precipitates present in substrate of N500 Specimen. These results provide us with an attractive method of combining nitriding with secondary hardening to achieve a comprehensive performance from surface to substrate of M50 steel.