The Experts below are selected from a list of 5121 Experts worldwide ranked by ideXlab platform
Meilang Xue - One of the best experts on this subject based on the ideXlab platform.
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activated protein c apc can increase bone anabolism via a protease activated receptor par 1 2 dependent mechanism
Journal of Orthopaedic Research, 2014Co-Authors: Kaitlin Shen, Ciara M Murphy, B Chan, Mille Kolind, Tegan L Cheng, Kathy Mikulec, Lauren Peacock, Meilang XueAbstract:Activated Protein C (APC) is an anticoagulant with strong cytoprotective properties that has been shown to promote wound healing. In this study APC was investigated for its potential Orthopedic Application using a Bone Morphogenetic Protein 2 (rhBMP-2) induced ectopic bone formation model. Local co-administration of 10 µg rhBMP-2 with 10 µg or 25 µg APC increased bone volume at 3 weeks by 32% (N.S.) and 74% (p<0.01) compared to rhBMP-2 alone. This was associated with a significant increase in CD31+ and TRAP+ cells in tissue sections of ectopic bone, consistent with enhanced vascularity and bone turnover. The actions of APC are largely mediated by its receptors endothelial protein C receptor (EPCR) and protease-activated receptors (PARs). Cultured pre-osteoblasts and bone nodule tissue sections were shown to express PAR1/2 and EPCR. When pre-osteoblasts were treated with APC, cell viability and phosphorylation of ERK1/2, Akt, and p38 were increased. Inhibition with PAR1 and sometimes PAR2 antagonists, but not with EPCR blocking antibodies, ameliorated the effects of APC on cell viability and kinase phosphorylation. These data indicate that APC can affect osteoblast viability and signaling, and may have in vivo Applications with rhBMP-2 for bone repair.
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activated protein c apc can increase bone anabolism via a protease activated receptor par 1 2 dependent mechanism
Journal of Orthopaedic Research, 2014Co-Authors: Kaitlin Shen, Ciara M Murphy, B Chan, Mille Kolind, Tegan L Cheng, Kathy Mikulec, Lauren Peacock, Meilang XueAbstract:Activated Protein C (APC) is an anticoagulant with strong cytoprotective properties that has been shown to promote wound healing. In this study APC was investigated for its potential Orthopedic Application using a Bone Morphogenetic Protein 2 (rhBMP-2) induced ectopic bone formation model. Local co-administration of 10 µg rhBMP-2 with 10 µg or 25 µg APC increased bone volume at 3 weeks by 32% (N.S.) and 74% (p < 0.01) compared to rhBMP-2 alone. This was associated with a significant increase in CD31+ and TRAP+ cells in tissue sections of ectopic bone, consistent with enhanced vascularity and bone turnover. The actions of APC are largely mediated by its receptors endothelial protein C receptor (EPCR) and protease-activated receptors (PARs). Cultured pre-osteoblasts and bone nodule tissue sections were shown to express PAR1/2 and EPCR. When pre-osteoblasts were treated with APC, cell viability and phosphorylation of ERK1/2, Akt, and p38 were increased. Inhibition with PAR1 and sometimes PAR2 antagonists, but not with EPCR blocking antibodies, ameliorated the effects of APC on cell viability and kinase phosphorylation. These data indicate that APC can affect osteoblast viability and signaling, and may have in vivo Applications with rhBMP-2 for bone repair. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:1549–1556, 2014.
Ciara M Murphy - One of the best experts on this subject based on the ideXlab platform.
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activated protein c apc can increase bone anabolism via a protease activated receptor par 1 2 dependent mechanism
Journal of Orthopaedic Research, 2014Co-Authors: Kaitlin Shen, Ciara M Murphy, B Chan, Mille Kolind, Tegan L Cheng, Kathy Mikulec, Lauren Peacock, Meilang XueAbstract:Activated Protein C (APC) is an anticoagulant with strong cytoprotective properties that has been shown to promote wound healing. In this study APC was investigated for its potential Orthopedic Application using a Bone Morphogenetic Protein 2 (rhBMP-2) induced ectopic bone formation model. Local co-administration of 10 µg rhBMP-2 with 10 µg or 25 µg APC increased bone volume at 3 weeks by 32% (N.S.) and 74% (p<0.01) compared to rhBMP-2 alone. This was associated with a significant increase in CD31+ and TRAP+ cells in tissue sections of ectopic bone, consistent with enhanced vascularity and bone turnover. The actions of APC are largely mediated by its receptors endothelial protein C receptor (EPCR) and protease-activated receptors (PARs). Cultured pre-osteoblasts and bone nodule tissue sections were shown to express PAR1/2 and EPCR. When pre-osteoblasts were treated with APC, cell viability and phosphorylation of ERK1/2, Akt, and p38 were increased. Inhibition with PAR1 and sometimes PAR2 antagonists, but not with EPCR blocking antibodies, ameliorated the effects of APC on cell viability and kinase phosphorylation. These data indicate that APC can affect osteoblast viability and signaling, and may have in vivo Applications with rhBMP-2 for bone repair.
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activated protein c apc can increase bone anabolism via a protease activated receptor par 1 2 dependent mechanism
Journal of Orthopaedic Research, 2014Co-Authors: Kaitlin Shen, Ciara M Murphy, B Chan, Mille Kolind, Tegan L Cheng, Kathy Mikulec, Lauren Peacock, Meilang XueAbstract:Activated Protein C (APC) is an anticoagulant with strong cytoprotective properties that has been shown to promote wound healing. In this study APC was investigated for its potential Orthopedic Application using a Bone Morphogenetic Protein 2 (rhBMP-2) induced ectopic bone formation model. Local co-administration of 10 µg rhBMP-2 with 10 µg or 25 µg APC increased bone volume at 3 weeks by 32% (N.S.) and 74% (p < 0.01) compared to rhBMP-2 alone. This was associated with a significant increase in CD31+ and TRAP+ cells in tissue sections of ectopic bone, consistent with enhanced vascularity and bone turnover. The actions of APC are largely mediated by its receptors endothelial protein C receptor (EPCR) and protease-activated receptors (PARs). Cultured pre-osteoblasts and bone nodule tissue sections were shown to express PAR1/2 and EPCR. When pre-osteoblasts were treated with APC, cell viability and phosphorylation of ERK1/2, Akt, and p38 were increased. Inhibition with PAR1 and sometimes PAR2 antagonists, but not with EPCR blocking antibodies, ameliorated the effects of APC on cell viability and kinase phosphorylation. These data indicate that APC can affect osteoblast viability and signaling, and may have in vivo Applications with rhBMP-2 for bone repair. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:1549–1556, 2014.
Tegan L Cheng - One of the best experts on this subject based on the ideXlab platform.
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activated protein c apc can increase bone anabolism via a protease activated receptor par 1 2 dependent mechanism
Journal of Orthopaedic Research, 2014Co-Authors: Kaitlin Shen, Ciara M Murphy, B Chan, Mille Kolind, Tegan L Cheng, Kathy Mikulec, Lauren Peacock, Meilang XueAbstract:Activated Protein C (APC) is an anticoagulant with strong cytoprotective properties that has been shown to promote wound healing. In this study APC was investigated for its potential Orthopedic Application using a Bone Morphogenetic Protein 2 (rhBMP-2) induced ectopic bone formation model. Local co-administration of 10 µg rhBMP-2 with 10 µg or 25 µg APC increased bone volume at 3 weeks by 32% (N.S.) and 74% (p<0.01) compared to rhBMP-2 alone. This was associated with a significant increase in CD31+ and TRAP+ cells in tissue sections of ectopic bone, consistent with enhanced vascularity and bone turnover. The actions of APC are largely mediated by its receptors endothelial protein C receptor (EPCR) and protease-activated receptors (PARs). Cultured pre-osteoblasts and bone nodule tissue sections were shown to express PAR1/2 and EPCR. When pre-osteoblasts were treated with APC, cell viability and phosphorylation of ERK1/2, Akt, and p38 were increased. Inhibition with PAR1 and sometimes PAR2 antagonists, but not with EPCR blocking antibodies, ameliorated the effects of APC on cell viability and kinase phosphorylation. These data indicate that APC can affect osteoblast viability and signaling, and may have in vivo Applications with rhBMP-2 for bone repair.
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activated protein c apc can increase bone anabolism via a protease activated receptor par 1 2 dependent mechanism
Journal of Orthopaedic Research, 2014Co-Authors: Kaitlin Shen, Ciara M Murphy, B Chan, Mille Kolind, Tegan L Cheng, Kathy Mikulec, Lauren Peacock, Meilang XueAbstract:Activated Protein C (APC) is an anticoagulant with strong cytoprotective properties that has been shown to promote wound healing. In this study APC was investigated for its potential Orthopedic Application using a Bone Morphogenetic Protein 2 (rhBMP-2) induced ectopic bone formation model. Local co-administration of 10 µg rhBMP-2 with 10 µg or 25 µg APC increased bone volume at 3 weeks by 32% (N.S.) and 74% (p < 0.01) compared to rhBMP-2 alone. This was associated with a significant increase in CD31+ and TRAP+ cells in tissue sections of ectopic bone, consistent with enhanced vascularity and bone turnover. The actions of APC are largely mediated by its receptors endothelial protein C receptor (EPCR) and protease-activated receptors (PARs). Cultured pre-osteoblasts and bone nodule tissue sections were shown to express PAR1/2 and EPCR. When pre-osteoblasts were treated with APC, cell viability and phosphorylation of ERK1/2, Akt, and p38 were increased. Inhibition with PAR1 and sometimes PAR2 antagonists, but not with EPCR blocking antibodies, ameliorated the effects of APC on cell viability and kinase phosphorylation. These data indicate that APC can affect osteoblast viability and signaling, and may have in vivo Applications with rhBMP-2 for bone repair. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:1549–1556, 2014.
Kaitlin Shen - One of the best experts on this subject based on the ideXlab platform.
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activated protein c apc can increase bone anabolism via a protease activated receptor par 1 2 dependent mechanism
Journal of Orthopaedic Research, 2014Co-Authors: Kaitlin Shen, Ciara M Murphy, B Chan, Mille Kolind, Tegan L Cheng, Kathy Mikulec, Lauren Peacock, Meilang XueAbstract:Activated Protein C (APC) is an anticoagulant with strong cytoprotective properties that has been shown to promote wound healing. In this study APC was investigated for its potential Orthopedic Application using a Bone Morphogenetic Protein 2 (rhBMP-2) induced ectopic bone formation model. Local co-administration of 10 µg rhBMP-2 with 10 µg or 25 µg APC increased bone volume at 3 weeks by 32% (N.S.) and 74% (p<0.01) compared to rhBMP-2 alone. This was associated with a significant increase in CD31+ and TRAP+ cells in tissue sections of ectopic bone, consistent with enhanced vascularity and bone turnover. The actions of APC are largely mediated by its receptors endothelial protein C receptor (EPCR) and protease-activated receptors (PARs). Cultured pre-osteoblasts and bone nodule tissue sections were shown to express PAR1/2 and EPCR. When pre-osteoblasts were treated with APC, cell viability and phosphorylation of ERK1/2, Akt, and p38 were increased. Inhibition with PAR1 and sometimes PAR2 antagonists, but not with EPCR blocking antibodies, ameliorated the effects of APC on cell viability and kinase phosphorylation. These data indicate that APC can affect osteoblast viability and signaling, and may have in vivo Applications with rhBMP-2 for bone repair.
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activated protein c apc can increase bone anabolism via a protease activated receptor par 1 2 dependent mechanism
Journal of Orthopaedic Research, 2014Co-Authors: Kaitlin Shen, Ciara M Murphy, B Chan, Mille Kolind, Tegan L Cheng, Kathy Mikulec, Lauren Peacock, Meilang XueAbstract:Activated Protein C (APC) is an anticoagulant with strong cytoprotective properties that has been shown to promote wound healing. In this study APC was investigated for its potential Orthopedic Application using a Bone Morphogenetic Protein 2 (rhBMP-2) induced ectopic bone formation model. Local co-administration of 10 µg rhBMP-2 with 10 µg or 25 µg APC increased bone volume at 3 weeks by 32% (N.S.) and 74% (p < 0.01) compared to rhBMP-2 alone. This was associated with a significant increase in CD31+ and TRAP+ cells in tissue sections of ectopic bone, consistent with enhanced vascularity and bone turnover. The actions of APC are largely mediated by its receptors endothelial protein C receptor (EPCR) and protease-activated receptors (PARs). Cultured pre-osteoblasts and bone nodule tissue sections were shown to express PAR1/2 and EPCR. When pre-osteoblasts were treated with APC, cell viability and phosphorylation of ERK1/2, Akt, and p38 were increased. Inhibition with PAR1 and sometimes PAR2 antagonists, but not with EPCR blocking antibodies, ameliorated the effects of APC on cell viability and kinase phosphorylation. These data indicate that APC can affect osteoblast viability and signaling, and may have in vivo Applications with rhBMP-2 for bone repair. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:1549–1556, 2014.
Kwan Ha Shin - One of the best experts on this subject based on the ideXlab platform.
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use of a poly ether imide coating to improve corrosion resistance and biocompatibility of magnesium mg implant for Orthopedic Applications
Journal of Biomedical Materials Research Part A, 2013Co-Authors: Sang Bok Kim, Sung Mi Lee, Hyounee Kim, Kwan Ha Shin, Young Hag KohAbstract:This study investigated the utility of poly(ether imide) (PEI) coating for improving the corrosion resistance and biocompatibility of magnesium (Mg) implants for Orthopedic Application. In particular, the microstructure of the PEI coating layers was controlled by the adjustment of the temperature used to dry the spin-coated wet PEI films. When a wet PEI film was dried at 4°C, a relatively thick and porous coating layer was achieved as a result of an extensive exchange of the solvent with water in a moist environment. In contrast, when a wet PEI film was dried at 70°C, a relatively thin and dense layer was created due to the faster evaporation of the solvent with a negligible exchange of the solvent with water. The porous PEI coating layer showed higher stability than did the dense one when immersed in a simulated body fluid (SBF), which was presumably attributed to the formation of chemical bonding between the PEI and the Mg substrate. Both the porous and the dense PEI coated Mg specimens showed significantly improved in vitro biocompatibility, which were assessed in terms of cell attachment, proliferation and differentiation. However, interestingly, the dense PEI coating layer showed greater cell proliferation and differentiation than did the porous layer. .
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use of a poly ether imide coating to improve corrosion resistance and biocompatibility of magnesium mg implant for Orthopedic Applications
Journal of Biomedical Materials Research Part A, 2013Co-Authors: Ji Hoon Jo, Kwan Ha ShinAbstract:This study investigated the utility of poly(ether imide) (PEI) coating for improving the corrosion resistance and biocompatibility of magnesium (Mg) implants for Orthopedic Application. In particular, the microstructure of the PEI coating layers was controlled by the adjustment of the temperature used to dry the spin-coated wet PEI films. When a wet PEI film was dried at 4°C, a relatively thick and porous coating layer was achieved as a result of an extensive exchange of the solvent with water in a moist environment. In contrast, when a wet PEI film was dried at 70°C, a relatively thin and dense layer was created due to the faster evaporation of the solvent with a negligible exchange of the solvent with water. The porous PEI coating layer showed higher stability than did the dense one when immersed in a simulated body fluid (SBF), which was presumably attributed to the formation of chemical bonding between the PEI and the Mg substrate. Both the porous and the dense PEI coated Mg specimens showed significantly improved in vitro biocompatibility, which were assessed in terms of cell attachment, proliferation and differentiation. However, interestingly, the dense PEI coating layer showed greater cell proliferation and differentiation than did the porous layer. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.
