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Antonio Beltrán Rodríguez - One of the best experts on this subject based on the ideXlab platform.

Sebastian Sjöqvist - One of the best experts on this subject based on the ideXlab platform.

Rudolf Jaenisch - One of the best experts on this subject based on the ideXlab platform.

  • establishment of human pluripotent stem cell derived pancreatic β like cells in the mouse pancreas
    Proceedings of the National Academy of Sciences of the United States of America, 2018
    Co-Authors: Katherine J Wert, Dmitry Shvartsman, Douglas A. Melton, Rudolf Jaenisch
    Abstract:

    Type 1 diabetes is characterized by autoimmune destruction of β cells located in pancreatic islets. However, tractable in vivo models of human pancreatic β cells have been limited. Here, we generated xenogeneic human pancreatic β-like cells in the mouse pancreas by Orthotopic Transplantation of stem cell-derived β (SC-β) cells into the pancreas of neonatal mice. The engrafted β-like cells expressed β cell transcription factors and markers associated with functional maturity. Engrafted human cells recruited mouse endothelial cells, suggesting functional integration. Human insulin was detected in the blood circulation of transplanted mice for months after Transplantation and increased upon glucose stimulation. In addition to β-like cells, human cells expressing markers for other endocrine pancreas cell types, acinar cells, and pancreatic ductal cells were identified in the pancreata of transplanted mice, indicating that this approach allows studying other human pancreatic cell types in the mouse pancreas. Our results demonstrate that Orthotopic Transplantation of human SC-β cells into neonatal mice is an experimental platform that allows the generation of mice with human pancreatic β-like cells in the endogenous niche.

Mei Ling Lim - One of the best experts on this subject based on the ideXlab platform.

Greg Lemon - One of the best experts on this subject based on the ideXlab platform.