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Vittorio Venturi - One of the best experts on this subject based on the ideXlab platform.
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oryr is a luxr family protein involved in interkingdom signaling between pathogenic xanthomonas oryzae pv oryzae and rice
Journal of Bacteriology, 2009Co-Authors: Sara Ferluga, Vittorio VenturiAbstract:Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight in rice, contains a regulator that is encoded in the genome, designated OryR, which belongs to the N-acyl homoserine lactone (AHL)-dependent quorum-sensing LuxR subfamily of proteins. However, we previously reported that X. oryzae pv. oryzae does not make AHLs and does not possess a LuxI-family AHL synthase and that the OryR protein is solubilized by a compound present in rice. In this study we obtained further evidence that OryR interacts with a rice signal molecule (RSM) and that the OryR concentration increases when rice is infected with X. oryzae pv. oryzae. We also describe three OryR target promoters which are regulated differently: (i) the neighboring proline iminopeptidase (pip) virulence gene, which is positively regulated by OryR in the presence of the RSM; (ii) the oryR promoter, which is negatively autoregulated independent of the RSM; and (iii) the 1,4-β-cellobiosidase cbsA gene, which is positively regulated by OryR independent of the RSM. We also found that the RSM for OryR is small, is not related to AHLs, and is not able to activate the broad-range AHL biosensor Agrobacterium tumefaciens NT1(pZLQR). Furthermore, OryR does not regulate production of the quorum-sensing diffusible signal factor present in the genus Xanthomonas. Therefore, OryR has unique features and is an important regulator involved in interkingdom communication between the host and the pathogen.
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a luxr homologue of xanthomonas oryzae pv oryzae is required for optimal rice virulence
Molecular Plant Pathology, 2007Co-Authors: Sara Ferluga, J Bigirimana, Monica Hofte, Vittorio VenturiAbstract:SUMMARY In Gram-negative bacteria a typical quorum sensing (QS) system usually involves the production and response to acylated homoserine lactones (AHLs). An AHL QS system is most commonly mediated by a LuxI family AHL synthase and a LuxR family AHL response regulator. This study reports for the first time the presence of a LuxR family-type regulator in Xanthomonas oryzae pv. oryzae (Xoo), which has been designated as OryR. The primary structure of OryR contains the typical signature domains of AHL QS LuxR family response regulators: an AHL-binding and a HTH DNA binding motif. The oryR gene is conserved among 26 Xoo strains and is also present in the genomes of close relatives X. campestris pv. campestris and X. axonopodis pv. citri. Disrupting oryR in three Xoo strains resulted in a significant reduction of rice virulence. The wild-type Xoo strains do not seem to produce AHLs and analysis of the Xoo sequenced genomes did not reveal the presence of a LuxI-family AHL synthase. The OryR protein was shown to be induced by macerated rice and affected the production of two secreted proteins: a cell-wall-degrading cellobiosidase and a 20-kDa protein of unknown function. By expressing and purifying OryR it was then observed that it was solubilized when grown in the presence of rice extract indicating that there could be a molecule(s) in rice which binds OryR. The role of OryR as a possible in planta induced LuxR family regulator is discussed.
Sara Ferluga - One of the best experts on this subject based on the ideXlab platform.
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oryr is a luxr family protein involved in interkingdom signaling between pathogenic xanthomonas oryzae pv oryzae and rice
Journal of Bacteriology, 2009Co-Authors: Sara Ferluga, Vittorio VenturiAbstract:Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight in rice, contains a regulator that is encoded in the genome, designated OryR, which belongs to the N-acyl homoserine lactone (AHL)-dependent quorum-sensing LuxR subfamily of proteins. However, we previously reported that X. oryzae pv. oryzae does not make AHLs and does not possess a LuxI-family AHL synthase and that the OryR protein is solubilized by a compound present in rice. In this study we obtained further evidence that OryR interacts with a rice signal molecule (RSM) and that the OryR concentration increases when rice is infected with X. oryzae pv. oryzae. We also describe three OryR target promoters which are regulated differently: (i) the neighboring proline iminopeptidase (pip) virulence gene, which is positively regulated by OryR in the presence of the RSM; (ii) the oryR promoter, which is negatively autoregulated independent of the RSM; and (iii) the 1,4-β-cellobiosidase cbsA gene, which is positively regulated by OryR independent of the RSM. We also found that the RSM for OryR is small, is not related to AHLs, and is not able to activate the broad-range AHL biosensor Agrobacterium tumefaciens NT1(pZLQR). Furthermore, OryR does not regulate production of the quorum-sensing diffusible signal factor present in the genus Xanthomonas. Therefore, OryR has unique features and is an important regulator involved in interkingdom communication between the host and the pathogen.
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a luxr homologue of xanthomonas oryzae pv oryzae is required for optimal rice virulence
Molecular Plant Pathology, 2007Co-Authors: Sara Ferluga, J Bigirimana, Monica Hofte, Vittorio VenturiAbstract:SUMMARY In Gram-negative bacteria a typical quorum sensing (QS) system usually involves the production and response to acylated homoserine lactones (AHLs). An AHL QS system is most commonly mediated by a LuxI family AHL synthase and a LuxR family AHL response regulator. This study reports for the first time the presence of a LuxR family-type regulator in Xanthomonas oryzae pv. oryzae (Xoo), which has been designated as OryR. The primary structure of OryR contains the typical signature domains of AHL QS LuxR family response regulators: an AHL-binding and a HTH DNA binding motif. The oryR gene is conserved among 26 Xoo strains and is also present in the genomes of close relatives X. campestris pv. campestris and X. axonopodis pv. citri. Disrupting oryR in three Xoo strains resulted in a significant reduction of rice virulence. The wild-type Xoo strains do not seem to produce AHLs and analysis of the Xoo sequenced genomes did not reveal the presence of a LuxI-family AHL synthase. The OryR protein was shown to be induced by macerated rice and affected the production of two secreted proteins: a cell-wall-degrading cellobiosidase and a 20-kDa protein of unknown function. By expressing and purifying OryR it was then observed that it was solubilized when grown in the presence of rice extract indicating that there could be a molecule(s) in rice which binds OryR. The role of OryR as a possible in planta induced LuxR family regulator is discussed.
Patrick W. Wargute - One of the best experts on this subject based on the ideXlab platform.
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Percentage changes in numbers of warthog, lesser kudu, Thomson’s gazelle, eland, Oryx, topi, hartebeest, impala, Grevy’s zebra and waterbuck in each of the 21 rangeland counties between 1977–1980 and 2011–2016.
2016Co-Authors: Hans-peter Piepho, Mohamed Y. Said, Gordon O. Ojwang, Lucy W. Njino, Shem C. Kifugo, Patrick W. WarguteAbstract:Percentage changes in numbers of warthog, lesser kudu, Thomson’s gazelle, eland, Oryx, topi, hartebeest, impala, Grevy’s zebra and waterbuck in each of the 21 rangeland counties between 1977–1980 and 2011–2016.
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The distribution of the proportion of the total biomass of warthog, lesser kudu, Thomson’s gazelle, eland, Oryx, topi, hartebeest, impala, Grevy’s zebra and waterbuck among the 21 rangeland counties of Kenya during 1977–80 and 2011–2016.
2016Co-Authors: Hans-peter Piepho, Mohamed Y. Said, Gordon O. Ojwang, Lucy W. Njino, Shem C. Kifugo, Patrick W. WarguteAbstract:The distribution of the proportion of the total biomass of warthog, lesser kudu, Thomson’s gazelle, eland, Oryx, topi, hartebeest, impala, Grevy’s zebra and waterbuck among the 21 rangeland counties of Kenya during 1977–80 and 2011–2016.
Hans-peter Piepho - One of the best experts on this subject based on the ideXlab platform.
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Trends in the density (Number /km2) of hyena, baboon, Grant’s gazelle, Grevy’s zebra, lion, spring hare, Burchell’s zebra, bushpig, white rhino, rock hyrax, topi and Oryx for the entire Nakuru Wildlife Conservancy during 1996–2015.
2017Co-Authors: Joseph O. Ogutu, Hans-peter Piepho, Bernard Kuloba, Erustus KangaAbstract:Trends in the density (Number /km2) of hyena, baboon, Grant’s gazelle, Grevy’s zebra, lion, spring hare, Burchell’s zebra, bushpig, white rhino, rock hyrax, topi and Oryx for the entire Nakuru Wildlife Conservancy during 1996–2015.
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Percentage changes in numbers of warthog, lesser kudu, Thomson’s gazelle, eland, Oryx, topi, hartebeest, impala, Grevy’s zebra and waterbuck in each of the 21 rangeland counties between 1977–1980 and 2011–2016.
2016Co-Authors: Hans-peter Piepho, Mohamed Y. Said, Gordon O. Ojwang, Lucy W. Njino, Shem C. Kifugo, Patrick W. WarguteAbstract:Percentage changes in numbers of warthog, lesser kudu, Thomson’s gazelle, eland, Oryx, topi, hartebeest, impala, Grevy’s zebra and waterbuck in each of the 21 rangeland counties between 1977–1980 and 2011–2016.
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The distribution of the proportion of the total biomass of warthog, lesser kudu, Thomson’s gazelle, eland, Oryx, topi, hartebeest, impala, Grevy’s zebra and waterbuck among the 21 rangeland counties of Kenya during 1977–80 and 2011–2016.
2016Co-Authors: Hans-peter Piepho, Mohamed Y. Said, Gordon O. Ojwang, Lucy W. Njino, Shem C. Kifugo, Patrick W. WarguteAbstract:The distribution of the proportion of the total biomass of warthog, lesser kudu, Thomson’s gazelle, eland, Oryx, topi, hartebeest, impala, Grevy’s zebra and waterbuck among the 21 rangeland counties of Kenya during 1977–80 and 2011–2016.
Kensuke Suzuki - One of the best experts on this subject based on the ideXlab platform.
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embolization of a spinal dural arteriovenous fistula with ethylene vinyl alcohol copolymer onyx using a dual lumen microballoon catheter and buddy wire technique
Surgical Neurology International, 2017Co-Authors: Ryuta Nakae, Masaya Nagaishi, Akio Hyodo, Kensuke SuzukiAbstract:Background N-butyl 2-cyanoacrylate (NBCA) remains the standard embolic agent for spinal dural arteriovenous fistula (SDAVF) treatment. Treatment of SDAVF with ethylene-vinyl alcohol copolymer (Onyx, ev3-Covidien, Irvine CA, USA) is currently not well established. Although several cases have reported the use of Onyx to embolize an intracranial dural arteriovenous fistula using a dual-lumen microballoon catheter, Onyx embolization of an SDAVF using a dual-lumen microballoon catheter has not been reported. Case description We treated a 57-year-old man with an SDAVF using a dual-lumen microballoon catheter and buddy wire technique to perform transarterial Onyx embolization via the left sixth intercostal artery. Conclusions Onyx embolization using a dual-lumen microballoon catheter was effective. Furthermore, the buddy wire technique was useful for providing rigid support of the microcatheter in a narrow and tortuous intercostal artery.
