The Experts below are selected from a list of 324 Experts worldwide ranked by ideXlab platform

Doug Brown - One of the best experts on this subject based on the ideXlab platform.

  • MGOS: A Resource for Studying Magnaporthe grisea and Oryza sativa Interactions
    Molecular Plant-microbe Interactions, 2006
    Co-Authors: Carol Soderlund, Karl Haller, Daniel J. Ebbole, Mark L. Farman, Marc Joel Orbach, Jinrong Xu, Guo Liang Wang, Vishal Pampanwar, Rod A Wing, Doug Brown
    Abstract:

    The MGOS (Magnaporthe grisea Oryza sativa) web-based database contains data from Oryza sativa and Magnaporthe grisea interaction experiments in which M. grisea is the fungal pathogen that causes the rice blast disease. In order to study the interactions, a consortium of fungal and rice geneticists was formed to construct a comprehensive set of experiments that would elucidate information about the gene expression of both rice and M. grisea during the infection cycle. These experiments included constructing and sequencing cDNA and robust long-serial analysis gene expression libraries from both host and pathogen during different stages of infection in both resistant and susceptible interactions, generating >50,000 M. grisea mutants and applying them to susceptible rice strains to test for pathogenicity, and constructing a dual O. sativa-M. grisea microarray. MGOS was developed as a central web-based repository for all the experimental data along with the rice and M. grisea genomic sequence. Community-based ...

  • MGOS: A resource for studying Magnaporthe grisea and Oryza sativa interactions.
    Molecular plant-microbe interactions : MPMI, 2006
    Co-Authors: Carol Soderlund, Karl Haller, Daniel J. Ebbole, Mark L. Farman, Marc Joel Orbach, Guo Liang Wang, Vishal Pampanwar, Rod A Wing, Doug Brown
    Abstract:

    The MGOS (Magnaporthe grisea Oryza sativa) web-based database contains data from Oryza sativa and Magnaporthe grisea interaction experiments in which M. grisea is the fungal pathogen that causes the rice blast disease. In order to study the interactions, a consortium of fungal and rice geneticists was formed to construct a comprehensive set of experiments that would elucidate information about the gene expression of both rice and M. grisea during the infection cycle. These experiments included constructing and sequencing cDNA and robust long-serial analysis gene expression libraries from both host and pathogen during different stages of infection in both resistant and susceptible interactions, generating >50,000 M. grisea mutants and applying them to susceptible rice strains to test for pathogenicity, and constructing a dual O. sativa-M. grisea microarray. MGOS was developed as a central web-based repository for all the experimental data along with the rice and M. grisea genomic sequence. Community-based annotation is available for the M. grisea genes to aid in the study of the interactions.

Guo Liang Wang - One of the best experts on this subject based on the ideXlab platform.

  • rice Oryza sativa protoplast isolation and its application for transient expression analysis
    Current protocols in plant biology, 2016
    Co-Authors: Feng He, Songbiao Chen, Yuese Ning, Guo Liang Wang
    Abstract:

    Rice (Oryza sativa) is not only the staple food for half of the world's population but also a model monocot plant for molecular biology studies. Although rice genes have been extensively investigated in the last two decades, the functions of many genes in the rice genome are still not known. One of the rapid and efficient approaches for determining gene function in vivo is protoplast-based transient expression analysis. We established a rice protoplast system about 10 years ago, which has been recently used in many laboratories. This protocol is useful for protein expression, subcellular localization, bimolecular fluorescence complementation, and co-immunoprecipitation assays. © 2016 by John Wiley & Sons, Inc. Keywords: rice (Oryza sativa); protoplast isolation; transient expression; BiFC and Co-IP

  • MGOS: A Resource for Studying Magnaporthe grisea and Oryza sativa Interactions
    Molecular Plant-microbe Interactions, 2006
    Co-Authors: Carol Soderlund, Karl Haller, Daniel J. Ebbole, Mark L. Farman, Marc Joel Orbach, Jinrong Xu, Guo Liang Wang, Vishal Pampanwar, Rod A Wing, Doug Brown
    Abstract:

    The MGOS (Magnaporthe grisea Oryza sativa) web-based database contains data from Oryza sativa and Magnaporthe grisea interaction experiments in which M. grisea is the fungal pathogen that causes the rice blast disease. In order to study the interactions, a consortium of fungal and rice geneticists was formed to construct a comprehensive set of experiments that would elucidate information about the gene expression of both rice and M. grisea during the infection cycle. These experiments included constructing and sequencing cDNA and robust long-serial analysis gene expression libraries from both host and pathogen during different stages of infection in both resistant and susceptible interactions, generating >50,000 M. grisea mutants and applying them to susceptible rice strains to test for pathogenicity, and constructing a dual O. sativa-M. grisea microarray. MGOS was developed as a central web-based repository for all the experimental data along with the rice and M. grisea genomic sequence. Community-based ...

  • MGOS: A resource for studying Magnaporthe grisea and Oryza sativa interactions.
    Molecular plant-microbe interactions : MPMI, 2006
    Co-Authors: Carol Soderlund, Karl Haller, Daniel J. Ebbole, Mark L. Farman, Marc Joel Orbach, Guo Liang Wang, Vishal Pampanwar, Rod A Wing, Doug Brown
    Abstract:

    The MGOS (Magnaporthe grisea Oryza sativa) web-based database contains data from Oryza sativa and Magnaporthe grisea interaction experiments in which M. grisea is the fungal pathogen that causes the rice blast disease. In order to study the interactions, a consortium of fungal and rice geneticists was formed to construct a comprehensive set of experiments that would elucidate information about the gene expression of both rice and M. grisea during the infection cycle. These experiments included constructing and sequencing cDNA and robust long-serial analysis gene expression libraries from both host and pathogen during different stages of infection in both resistant and susceptible interactions, generating >50,000 M. grisea mutants and applying them to susceptible rice strains to test for pathogenicity, and constructing a dual O. sativa-M. grisea microarray. MGOS was developed as a central web-based repository for all the experimental data along with the rice and M. grisea genomic sequence. Community-based annotation is available for the M. grisea genes to aid in the study of the interactions.

Carol Soderlund - One of the best experts on this subject based on the ideXlab platform.

  • MGOS: A Resource for Studying Magnaporthe grisea and Oryza sativa Interactions
    Molecular Plant-microbe Interactions, 2006
    Co-Authors: Carol Soderlund, Karl Haller, Daniel J. Ebbole, Mark L. Farman, Marc Joel Orbach, Jinrong Xu, Guo Liang Wang, Vishal Pampanwar, Rod A Wing, Doug Brown
    Abstract:

    The MGOS (Magnaporthe grisea Oryza sativa) web-based database contains data from Oryza sativa and Magnaporthe grisea interaction experiments in which M. grisea is the fungal pathogen that causes the rice blast disease. In order to study the interactions, a consortium of fungal and rice geneticists was formed to construct a comprehensive set of experiments that would elucidate information about the gene expression of both rice and M. grisea during the infection cycle. These experiments included constructing and sequencing cDNA and robust long-serial analysis gene expression libraries from both host and pathogen during different stages of infection in both resistant and susceptible interactions, generating >50,000 M. grisea mutants and applying them to susceptible rice strains to test for pathogenicity, and constructing a dual O. sativa-M. grisea microarray. MGOS was developed as a central web-based repository for all the experimental data along with the rice and M. grisea genomic sequence. Community-based ...

  • MGOS: A resource for studying Magnaporthe grisea and Oryza sativa interactions.
    Molecular plant-microbe interactions : MPMI, 2006
    Co-Authors: Carol Soderlund, Karl Haller, Daniel J. Ebbole, Mark L. Farman, Marc Joel Orbach, Guo Liang Wang, Vishal Pampanwar, Rod A Wing, Doug Brown
    Abstract:

    The MGOS (Magnaporthe grisea Oryza sativa) web-based database contains data from Oryza sativa and Magnaporthe grisea interaction experiments in which M. grisea is the fungal pathogen that causes the rice blast disease. In order to study the interactions, a consortium of fungal and rice geneticists was formed to construct a comprehensive set of experiments that would elucidate information about the gene expression of both rice and M. grisea during the infection cycle. These experiments included constructing and sequencing cDNA and robust long-serial analysis gene expression libraries from both host and pathogen during different stages of infection in both resistant and susceptible interactions, generating >50,000 M. grisea mutants and applying them to susceptible rice strains to test for pathogenicity, and constructing a dual O. sativa-M. grisea microarray. MGOS was developed as a central web-based repository for all the experimental data along with the rice and M. grisea genomic sequence. Community-based annotation is available for the M. grisea genes to aid in the study of the interactions.

Yu-xi Feng - One of the best experts on this subject based on the ideXlab platform.

Mark L. Farman - One of the best experts on this subject based on the ideXlab platform.

  • MGOS: A Resource for Studying Magnaporthe grisea and Oryza sativa Interactions
    Molecular Plant-microbe Interactions, 2006
    Co-Authors: Carol Soderlund, Karl Haller, Daniel J. Ebbole, Mark L. Farman, Marc Joel Orbach, Jinrong Xu, Guo Liang Wang, Vishal Pampanwar, Rod A Wing, Doug Brown
    Abstract:

    The MGOS (Magnaporthe grisea Oryza sativa) web-based database contains data from Oryza sativa and Magnaporthe grisea interaction experiments in which M. grisea is the fungal pathogen that causes the rice blast disease. In order to study the interactions, a consortium of fungal and rice geneticists was formed to construct a comprehensive set of experiments that would elucidate information about the gene expression of both rice and M. grisea during the infection cycle. These experiments included constructing and sequencing cDNA and robust long-serial analysis gene expression libraries from both host and pathogen during different stages of infection in both resistant and susceptible interactions, generating >50,000 M. grisea mutants and applying them to susceptible rice strains to test for pathogenicity, and constructing a dual O. sativa-M. grisea microarray. MGOS was developed as a central web-based repository for all the experimental data along with the rice and M. grisea genomic sequence. Community-based ...

  • MGOS: A resource for studying Magnaporthe grisea and Oryza sativa interactions.
    Molecular plant-microbe interactions : MPMI, 2006
    Co-Authors: Carol Soderlund, Karl Haller, Daniel J. Ebbole, Mark L. Farman, Marc Joel Orbach, Guo Liang Wang, Vishal Pampanwar, Rod A Wing, Doug Brown
    Abstract:

    The MGOS (Magnaporthe grisea Oryza sativa) web-based database contains data from Oryza sativa and Magnaporthe grisea interaction experiments in which M. grisea is the fungal pathogen that causes the rice blast disease. In order to study the interactions, a consortium of fungal and rice geneticists was formed to construct a comprehensive set of experiments that would elucidate information about the gene expression of both rice and M. grisea during the infection cycle. These experiments included constructing and sequencing cDNA and robust long-serial analysis gene expression libraries from both host and pathogen during different stages of infection in both resistant and susceptible interactions, generating >50,000 M. grisea mutants and applying them to susceptible rice strains to test for pathogenicity, and constructing a dual O. sativa-M. grisea microarray. MGOS was developed as a central web-based repository for all the experimental data along with the rice and M. grisea genomic sequence. Community-based annotation is available for the M. grisea genes to aid in the study of the interactions.