The Experts below are selected from a list of 42603 Experts worldwide ranked by ideXlab platform
Manoj Bhatt - One of the best experts on this subject based on the ideXlab platform.
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Transcriptional regulation of Osmotic Stress tolerance in wheat (Triticum aestivum L.).
Plant Molecular Biology, 2018Co-Authors: Shabir H. Wani, Prateek Tripathi, Abbu Zaid, Ghana S. Challa, Anuj Kumar, Vinay Kumar, Jyoti Upadhyay, Rohit Joshi, Manoj BhattAbstract:The current review provides an updated, new insights into the regulation of transcription mediated underlying mechanisms of wheat plants to Osmotic Stress perturbations. Osmotic Stress tolerance mechanisms being complex are governed by multiple factors at physiological, biochemical and at the molecular level, hence approaches like “OMICS” that can underpin mechanisms behind Osmotic tolerance in wheat is of paramount importance. The transcription factors (TFs) are a class of molecular proteins, which are involved in regulation, modulation and orchestrating the responses of plants to a variety of environmental Stresses. Recent reports have provided novel insights on the role of TFs in Osmotic Stress tolerance via direct molecular links. However, our knowledge on the regulatory role TFs during Osmotic Stress tolerance in wheat remains limited. The present review in its first part sheds light on the importance of studying the role of Osmotic Stress tolerance in wheat plants and second aims to decipher molecular mechanisms of TFs belonging to several classes, including DREB, NAC, MYB, WRKY and bHLH, which have been reported to engage in Osmotic Stress mediated gene expression in wheat and third part covers the systems biology approaches to understand the transcriptional regulation of Osmotic Stress and the role of long non-coding RNAs in response to Osmotic Stress with special emphasis on wheat. The current concept may lead to an understanding in molecular regulation and signalling interaction of TFs under Osmotic Stress to clarify challenges and problems for devising potential strategies to improve complex regulatory events involved in plant tolerance to Osmotic Stress adaptive pathways in wheat.
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Transcriptional regulation of Osmotic Stress tolerance in wheat (Triticum aestivum L.)
Plant Molecular Biology, 2018Co-Authors: Shabir H. Wani, Prateek Tripathi, Abbu Zaid, Ghana S. Challa, Anuj Kumar, Vinay Kumar, Jyoti Upadhyay, Rohit Joshi, Manoj BhattAbstract:Key message The current review provides an updated, new insights into the regulation of transcription mediated underlying mechanisms of wheat plants to Osmotic Stress perturbations. Abstract Osmotic Stress tolerance mechanisms being complex are governed by multiple factors at physiological, biochemical and at the molecular level, hence approaches like “OMICS” that can underpin mechanisms behind Osmotic tolerance in wheat is of paramount importance. The transcription factors (TFs) are a class of molecular proteins, which are involved in regulation, modulation and orchestrating the responses of plants to a variety of environmental Stresses. Recent reports have provided novel insights on the role of TFs in Osmotic Stress tolerance via direct molecular links. However, our knowledge on the regulatory role TFs during Osmotic Stress tolerance in wheat remains limited. The present review in its first part sheds light on the importance of studying the role of Osmotic Stress tolerance in wheat plants and second aims to decipher molecular mechanisms of TFs belonging to several classes, including DREB, NAC, MYB, WRKY and bHLH , which have been reported to engage in Osmotic Stress mediated gene expression in wheat and third part covers the systems biology approaches to understand the transcriptional regulation of Osmotic Stress and the role of long non-coding RNAs in response to Osmotic Stress with special emphasis on wheat. The current concept may lead to an understanding in molecular regulation and signalling interaction of TFs under Osmotic Stress to clarify challenges and problems for devising potential strategies to improve complex regulatory events involved in plant tolerance to Osmotic Stress adaptive pathways in wheat.
Manabu Ishitani - One of the best experts on this subject based on the ideXlab platform.
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regulation of Osmotic Stress responsive gene expression by the los6 aba1 locus in arabidopsis
Journal of Biological Chemistry, 2002Co-Authors: Liming Xiong, Manabu IshitaniAbstract:Abstract Drought and high salinity induce the expression of many plant genes. To understand the signal transduction mechanisms underlying the activation of these genes, we carried out a genetic screen to isolate Arabidopsis mutants defective in Osmotic Stress-regulated gene induction. Here we report the isolation, characterization, and cloning of a mutation, los6, which diminished Osmotic Stress activation of a reporter gene. RNA blot analysis indicates that under Osmotic Stress the transcript levels for Stress-responsive genes such as RD29A, COR15A,KIN1, COR47, RD19, andADH are lower in los6 plants than in wild type plants. los6 plants were found to have reduced phytohormone abscisic acid (ABA) accumulation and to be allelic to the ABA-deficient mutant, aba1. LOS6/ABA1 encodes a zeaxanthin epoxidase that functions in ABA biosynthesis. Its expression is enhanced by Osmotic Stress. Furthermore, we found that there exists a positive feedback regulation by ABA on the expression of LOS6/ABA1, which may underscore a quick adaptation strategy for plants under Osmotic Stress. Similar positive regulation by ABA also exists for other ABA biosynthesis genes AAO3 and LOS5/ABA3 and in certain genetic backgrounds, NCED3. This feedback regulation by ABA is impaired in the ABA-insensitive mutantabi1 but not in abi2. Moreover, the up-regulation of LOS6/ABA1, LOS5/ABA3,AAO3, and NCED3 by Osmotic Stress is reduced substantially in ABA-deficient mutants. Transgenic plants overexpressing LOS6/ABA1 showed an increasedRD29A-LUC expression under Osmotic Stress. These results suggest that the level of gene induction by Osmotic Stress is dependent on the dosage of the zeaxanthin epoxidase enzyme.
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Regulation of Osmotic Stress-responsive gene expression by the LOS6/ABA1 locus in Arabidopsis.
Journal of Biological Chemistry, 2002Co-Authors: Liming Xiong, Manabu IshitaniAbstract:Abstract Drought and high salinity induce the expression of many plant genes. To understand the signal transduction mechanisms underlying the activation of these genes, we carried out a genetic screen to isolate Arabidopsis mutants defective in Osmotic Stress-regulated gene induction. Here we report the isolation, characterization, and cloning of a mutation, los6, which diminished Osmotic Stress activation of a reporter gene. RNA blot analysis indicates that under Osmotic Stress the transcript levels for Stress-responsive genes such as RD29A, COR15A,KIN1, COR47, RD19, andADH are lower in los6 plants than in wild type plants. los6 plants were found to have reduced phytohormone abscisic acid (ABA) accumulation and to be allelic to the ABA-deficient mutant, aba1. LOS6/ABA1 encodes a zeaxanthin epoxidase that functions in ABA biosynthesis. Its expression is enhanced by Osmotic Stress. Furthermore, we found that there exists a positive feedback regulation by ABA on the expression of LOS6/ABA1, which may underscore a quick adaptation strategy for plants under Osmotic Stress. Similar positive regulation by ABA also exists for other ABA biosynthesis genes AAO3 and LOS5/ABA3 and in certain genetic backgrounds, NCED3. This feedback regulation by ABA is impaired in the ABA-insensitive mutantabi1 but not in abi2. Moreover, the up-regulation of LOS6/ABA1, LOS5/ABA3,AAO3, and NCED3 by Osmotic Stress is reduced substantially in ABA-deficient mutants. Transgenic plants overexpressing LOS6/ABA1 showed an increasedRD29A-LUC expression under Osmotic Stress. These results suggest that the level of gene induction by Osmotic Stress is dependent on the dosage of the zeaxanthin epoxidase enzyme.
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HOS5-a negative regulator of Osmotic Stress-induced gene expression in Arabidopsis thaliana.
Plant Journal, 1999Co-Authors: Liming Xiong, Manabu IshitaniAbstract:Summary Osmotic Stress activates the expression of many plant genes through ABA-dependent as well as ABAindependent signaling pathways. We report here the characterization of a novel mutant of Arabidopsis thaliana, hos5-1, which exhibits increased expression of the Osmotic Stress responsive RD29A gene. The expression of several other Stress genes are also enhanced by the hos5-1 mutation. The enhanced expression is specific to ABA and Osmotic Stress because low temperature regulation of these genes is not altered in the mutant. Genetic analysis indicated that hos5-1 is a recessive mutation in a single nuclear gene on chromosome III. Double mutant analysis of hos5-1 and the ABA-deficient aba1-1 as well as the ABA-insensitive abi1-1 mutant indicated that the Osmotic Stress hypersensitivity of hos5-1 is not affected by ABA deficiency or insensitivity. Furthermore, combined treatments of hos5-1 with ABA and Osmotic Stress had an additive effect on RD29A-LUC expression. These results suggest that the Osmotic Stress hypersensitivity in hos5-1 may be ABA-independent. The germination of hos5-1 seeds was more resistant to ABA. However, the hos5-1 mutation did not influence stomatal control and only slightly affected the regulation of growth and proline accumulation by ABA. The hos5-1 mutation reveals a negative regulator of Osmotic Stress-responsive gene expression shared by ABA-dependent and ABAindependent Osmotic Stress signaling pathways.
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Interaction of Osmotic Stress, Temperature, and Abscisic Acid in the Regulation of Gene Expression in Arabidopsis
Plant Physiology, 1999Co-Authors: Liming Xiong, Manabu IshitaniAbstract:The impact of simultaneous environmental Stresses on plants and how they respond to combined Stresses compared with single Stresses is largely unclear. By using a transgene (RD29A-LUC) consisting of the firefly luciferase coding sequence (LUC) driven by the Stress-responsive RD29A promoter, we investigated the interactive effects of temperature, Osmotic Stress, and the phytohormone abscisic acid (ABA) in the regulation of gene expression in Arabidopsis seedlings. Results indicated that both positive and negative interactions exist among the studied Stress factors in regulating gene expression. At a normal growth temperature (22°C), Osmotic Stress and ABA act synergistically to induce the transgene expression. Low temperature inhibits the response to Osmotic Stress or to combined treatment of Osmotic Stress and ABA, whereas low temperature and ABA treatments are additive in inducing transgene expression. Although high temperature alone does not activate the transgene, it significantly amplifies the effects of ABA and Osmotic Stress. The effect of multiple Stresses in the regulation of RD29A-LUC expression in signal transduction mutants was also studied. The results are discussed in the context of cold and Osmotic Stress signal transduction pathways.
Shabir H. Wani - One of the best experts on this subject based on the ideXlab platform.
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Transcriptional regulation of Osmotic Stress tolerance in wheat (Triticum aestivum L.).
Plant Molecular Biology, 2018Co-Authors: Shabir H. Wani, Prateek Tripathi, Abbu Zaid, Ghana S. Challa, Anuj Kumar, Vinay Kumar, Jyoti Upadhyay, Rohit Joshi, Manoj BhattAbstract:The current review provides an updated, new insights into the regulation of transcription mediated underlying mechanisms of wheat plants to Osmotic Stress perturbations. Osmotic Stress tolerance mechanisms being complex are governed by multiple factors at physiological, biochemical and at the molecular level, hence approaches like “OMICS” that can underpin mechanisms behind Osmotic tolerance in wheat is of paramount importance. The transcription factors (TFs) are a class of molecular proteins, which are involved in regulation, modulation and orchestrating the responses of plants to a variety of environmental Stresses. Recent reports have provided novel insights on the role of TFs in Osmotic Stress tolerance via direct molecular links. However, our knowledge on the regulatory role TFs during Osmotic Stress tolerance in wheat remains limited. The present review in its first part sheds light on the importance of studying the role of Osmotic Stress tolerance in wheat plants and second aims to decipher molecular mechanisms of TFs belonging to several classes, including DREB, NAC, MYB, WRKY and bHLH, which have been reported to engage in Osmotic Stress mediated gene expression in wheat and third part covers the systems biology approaches to understand the transcriptional regulation of Osmotic Stress and the role of long non-coding RNAs in response to Osmotic Stress with special emphasis on wheat. The current concept may lead to an understanding in molecular regulation and signalling interaction of TFs under Osmotic Stress to clarify challenges and problems for devising potential strategies to improve complex regulatory events involved in plant tolerance to Osmotic Stress adaptive pathways in wheat.
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Transcriptional regulation of Osmotic Stress tolerance in wheat (Triticum aestivum L.)
Plant Molecular Biology, 2018Co-Authors: Shabir H. Wani, Prateek Tripathi, Abbu Zaid, Ghana S. Challa, Anuj Kumar, Vinay Kumar, Jyoti Upadhyay, Rohit Joshi, Manoj BhattAbstract:Key message The current review provides an updated, new insights into the regulation of transcription mediated underlying mechanisms of wheat plants to Osmotic Stress perturbations. Abstract Osmotic Stress tolerance mechanisms being complex are governed by multiple factors at physiological, biochemical and at the molecular level, hence approaches like “OMICS” that can underpin mechanisms behind Osmotic tolerance in wheat is of paramount importance. The transcription factors (TFs) are a class of molecular proteins, which are involved in regulation, modulation and orchestrating the responses of plants to a variety of environmental Stresses. Recent reports have provided novel insights on the role of TFs in Osmotic Stress tolerance via direct molecular links. However, our knowledge on the regulatory role TFs during Osmotic Stress tolerance in wheat remains limited. The present review in its first part sheds light on the importance of studying the role of Osmotic Stress tolerance in wheat plants and second aims to decipher molecular mechanisms of TFs belonging to several classes, including DREB, NAC, MYB, WRKY and bHLH , which have been reported to engage in Osmotic Stress mediated gene expression in wheat and third part covers the systems biology approaches to understand the transcriptional regulation of Osmotic Stress and the role of long non-coding RNAs in response to Osmotic Stress with special emphasis on wheat. The current concept may lead to an understanding in molecular regulation and signalling interaction of TFs under Osmotic Stress to clarify challenges and problems for devising potential strategies to improve complex regulatory events involved in plant tolerance to Osmotic Stress adaptive pathways in wheat.
Liming Xiong - One of the best experts on this subject based on the ideXlab platform.
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The Arabidopsis Vacuolar Sorting Receptor1 Is Required for Osmotic Stress-Induced Abscisic Acid Biosynthesis
Plant Physiology, 2014Co-Authors: Zhen Yu Wang, Chris Gehring, Feng-min Li, Liming XiongAbstract:Osmotic Stress activates the biosynthesis of the phytohormone abscisic acid (ABA) through a pathway that is rate limited by the carotenoid cleavage enzyme 9-cis-epoxycarotenoid dioxygenase (NCED). To understand the signal transduction mechanism underlying the activation of ABA biosynthesis, we performed a forward genetic screen to isolate mutants defective in Osmotic Stress regulation of the NCED3 gene. Here, we identified the Arabidopsis (Arabidopsis thaliana) Vacuolar Sorting Receptor1 (VSR1) as a unique regulator of ABA biosynthesis. The vsr1 mutant not only shows increased sensitivity to Osmotic Stress, but also is defective in the feedback regulation of ABA biosynthesis by ABA. Further analysis revealed that vacuolar trafficking mediated by VSR1 is required for Osmotic Stress-responsive ABA biosynthesis and Osmotic Stress tolerance. Moreover, under Osmotic Stress conditions, the membrane potential, calcium flux, and vacuolar pH changes in the vsr1 mutant differ from those in the wild type. Given that manipulation of the intracellular pH is sufficient to modulate the expression of ABA biosynthesis genes, including NCED3, and ABA accumulation, we propose that intracellular pH changes caused by Osmotic Stress may play a signaling role in regulating ABA biosynthesis and that this regulation is dependent on functional VSR1.
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The Plant Cuticle Is Required for Osmotic Stress Regulation of Abscisic Acid Biosynthesis and Osmotic Stress Tolerance in Arabidopsis
The Plant Cell, 2011Co-Authors: Zhen Yu Wang, Liming Xiong, Wenbo LiAbstract:Osmotic Stress activates the biosynthesis of abscisic acid (ABA). One major step in ABA biosynthesis is the carotenoid cleavage catalyzed by a 9-cis epoxycarotenoid dioxygenase (NCED). To understand the mechanism for Osmotic Stress activation of ABA biosynthesis, we screened for Arabidopsis thaliana mutants that failed to induce the NCED3 gene expression in response to Osmotic Stress treatments. The ced1 (for 9-cis epoxycarotenoid dioxygenase defective 1) mutant isolated in this study showed markedly reduced expression of NCED3 in response to Osmotic Stress (polyethylene glycol) treatments compared with the wild type. Other ABA biosynthesis genes are also greatly reduced in ced1 under Osmotic Stress. ced1 mutant plants are very sensitive to even mild Osmotic Stress. Map-based cloning revealed unexpectedly that CED1 encodes a putative α/β hydrolase domain-containing protein and is allelic to the BODYGUARD gene that was recently shown to be essential for cuticle biogenesis. Further studies discovered that other cutin biosynthesis mutants are also impaired in Osmotic Stress induction of ABA biosynthesis genes and are sensitive to Osmotic Stress. Our work demonstrates that the cuticle functions not merely as a physical barrier to minimize water loss but also mediates Osmotic Stress signaling and tolerance by regulating ABA biosynthesis and signaling.
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regulation of Osmotic Stress responsive gene expression by the los6 aba1 locus in arabidopsis
Journal of Biological Chemistry, 2002Co-Authors: Liming Xiong, Manabu IshitaniAbstract:Abstract Drought and high salinity induce the expression of many plant genes. To understand the signal transduction mechanisms underlying the activation of these genes, we carried out a genetic screen to isolate Arabidopsis mutants defective in Osmotic Stress-regulated gene induction. Here we report the isolation, characterization, and cloning of a mutation, los6, which diminished Osmotic Stress activation of a reporter gene. RNA blot analysis indicates that under Osmotic Stress the transcript levels for Stress-responsive genes such as RD29A, COR15A,KIN1, COR47, RD19, andADH are lower in los6 plants than in wild type plants. los6 plants were found to have reduced phytohormone abscisic acid (ABA) accumulation and to be allelic to the ABA-deficient mutant, aba1. LOS6/ABA1 encodes a zeaxanthin epoxidase that functions in ABA biosynthesis. Its expression is enhanced by Osmotic Stress. Furthermore, we found that there exists a positive feedback regulation by ABA on the expression of LOS6/ABA1, which may underscore a quick adaptation strategy for plants under Osmotic Stress. Similar positive regulation by ABA also exists for other ABA biosynthesis genes AAO3 and LOS5/ABA3 and in certain genetic backgrounds, NCED3. This feedback regulation by ABA is impaired in the ABA-insensitive mutantabi1 but not in abi2. Moreover, the up-regulation of LOS6/ABA1, LOS5/ABA3,AAO3, and NCED3 by Osmotic Stress is reduced substantially in ABA-deficient mutants. Transgenic plants overexpressing LOS6/ABA1 showed an increasedRD29A-LUC expression under Osmotic Stress. These results suggest that the level of gene induction by Osmotic Stress is dependent on the dosage of the zeaxanthin epoxidase enzyme.
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Regulation of Osmotic Stress-responsive gene expression by the LOS6/ABA1 locus in Arabidopsis.
Journal of Biological Chemistry, 2002Co-Authors: Liming Xiong, Manabu IshitaniAbstract:Abstract Drought and high salinity induce the expression of many plant genes. To understand the signal transduction mechanisms underlying the activation of these genes, we carried out a genetic screen to isolate Arabidopsis mutants defective in Osmotic Stress-regulated gene induction. Here we report the isolation, characterization, and cloning of a mutation, los6, which diminished Osmotic Stress activation of a reporter gene. RNA blot analysis indicates that under Osmotic Stress the transcript levels for Stress-responsive genes such as RD29A, COR15A,KIN1, COR47, RD19, andADH are lower in los6 plants than in wild type plants. los6 plants were found to have reduced phytohormone abscisic acid (ABA) accumulation and to be allelic to the ABA-deficient mutant, aba1. LOS6/ABA1 encodes a zeaxanthin epoxidase that functions in ABA biosynthesis. Its expression is enhanced by Osmotic Stress. Furthermore, we found that there exists a positive feedback regulation by ABA on the expression of LOS6/ABA1, which may underscore a quick adaptation strategy for plants under Osmotic Stress. Similar positive regulation by ABA also exists for other ABA biosynthesis genes AAO3 and LOS5/ABA3 and in certain genetic backgrounds, NCED3. This feedback regulation by ABA is impaired in the ABA-insensitive mutantabi1 but not in abi2. Moreover, the up-regulation of LOS6/ABA1, LOS5/ABA3,AAO3, and NCED3 by Osmotic Stress is reduced substantially in ABA-deficient mutants. Transgenic plants overexpressing LOS6/ABA1 showed an increasedRD29A-LUC expression under Osmotic Stress. These results suggest that the level of gene induction by Osmotic Stress is dependent on the dosage of the zeaxanthin epoxidase enzyme.
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HOS5-a negative regulator of Osmotic Stress-induced gene expression in Arabidopsis thaliana.
Plant Journal, 1999Co-Authors: Liming Xiong, Manabu IshitaniAbstract:Summary Osmotic Stress activates the expression of many plant genes through ABA-dependent as well as ABAindependent signaling pathways. We report here the characterization of a novel mutant of Arabidopsis thaliana, hos5-1, which exhibits increased expression of the Osmotic Stress responsive RD29A gene. The expression of several other Stress genes are also enhanced by the hos5-1 mutation. The enhanced expression is specific to ABA and Osmotic Stress because low temperature regulation of these genes is not altered in the mutant. Genetic analysis indicated that hos5-1 is a recessive mutation in a single nuclear gene on chromosome III. Double mutant analysis of hos5-1 and the ABA-deficient aba1-1 as well as the ABA-insensitive abi1-1 mutant indicated that the Osmotic Stress hypersensitivity of hos5-1 is not affected by ABA deficiency or insensitivity. Furthermore, combined treatments of hos5-1 with ABA and Osmotic Stress had an additive effect on RD29A-LUC expression. These results suggest that the Osmotic Stress hypersensitivity in hos5-1 may be ABA-independent. The germination of hos5-1 seeds was more resistant to ABA. However, the hos5-1 mutation did not influence stomatal control and only slightly affected the regulation of growth and proline accumulation by ABA. The hos5-1 mutation reveals a negative regulator of Osmotic Stress-responsive gene expression shared by ABA-dependent and ABAindependent Osmotic Stress signaling pathways.
Kunliang Guan - One of the best experts on this subject based on the ideXlab platform.
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Osmotic Stress induced phosphorylation by nlk at ser128 activates yap
EMBO Reports, 2017Co-Authors: Audrey W Hong, Zhipeng Meng, Haixin Yuan, Steven W Plouffe, Sung Ho Moon, Kunliang GuanAbstract:Abstract YAP is the major downstream effector of the Hippo pathway, which controls cell growth, tissue homeostasis, and organ size. Aberrant YAP activation, resulting from dysregulation of the Hippo pathway, is frequently observed in human cancers. YAP is a transcription co‐activator, and the key mechanism of YAP regulation is its nuclear and cytoplasmic translocation. The Hippo pathway component, LATS, inhibits YAP by phosphorylating YAP at Ser127, leading to 14‐3‐3 binding and cytoplasmic retention of YAP. Here, we report that Osmotic Stress stimulates transient YAP nuclear localization and increases YAP activity even when YAP Ser127 is phosphorylated. Osmotic Stress acts via the NLK kinase to induce YAP Ser128 phosphorylation. Phosphorylation of YAP at Ser128 interferes with its ability to bind to 14‐3‐3, resulting in YAP nuclear accumulation and induction of downstream target gene expression. This Osmotic Stress‐induced YAP activation enhances cellular Stress adaptation. Our findings reveal a critical role for NLK‐mediated Ser128 phosphorylation in YAP regulation and a crosstalk between Osmotic Stress and the Hippo pathway.
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Osmotic Stress‐induced phosphorylation by NLK at Ser128 activates YAP
EMBO Reports, 2016Co-Authors: Audrey W Hong, Zhipeng Meng, Haixin Yuan, Steven W Plouffe, Sung Ho Moon, Kunliang GuanAbstract:Abstract YAP is the major downstream effector of the Hippo pathway, which controls cell growth, tissue homeostasis, and organ size. Aberrant YAP activation, resulting from dysregulation of the Hippo pathway, is frequently observed in human cancers. YAP is a transcription co‐activator, and the key mechanism of YAP regulation is its nuclear and cytoplasmic translocation. The Hippo pathway component, LATS, inhibits YAP by phosphorylating YAP at Ser127, leading to 14‐3‐3 binding and cytoplasmic retention of YAP. Here, we report that Osmotic Stress stimulates transient YAP nuclear localization and increases YAP activity even when YAP Ser127 is phosphorylated. Osmotic Stress acts via the NLK kinase to induce YAP Ser128 phosphorylation. Phosphorylation of YAP at Ser128 interferes with its ability to bind to 14‐3‐3, resulting in YAP nuclear accumulation and induction of downstream target gene expression. This Osmotic Stress‐induced YAP activation enhances cellular Stress adaptation. Our findings reveal a critical role for NLK‐mediated Ser128 phosphorylation in YAP regulation and a crosstalk between Osmotic Stress and the Hippo pathway.
