The Experts below are selected from a list of 12525 Experts worldwide ranked by ideXlab platform
Kuniyoshi Shimizu - One of the best experts on this subject based on the ideXlab platform.
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Ursolic Acid Isolated from the Leaves of Loquat ( Eriobotrya japonica) Inhibited Osteoclast Differentiation through Targeting Exportin 5.
Journal of agricultural and food chemistry, 2019Co-Authors: Hui Tan, Chong Zhao, Qinchang Zhu, Yoshinori Katakura, Hiroyuki Tanaka, Koichiro Ohnuki, Kuniyoshi ShimizuAbstract:One of the conventional strategies for treating osteoporosis is to eliminate the multinucleated Osteoclasts that are responsible for bone resorption. Our previous study revealed that ursolic acid, isolated from leaves of loquat that is used as tasty tea in Japan, suppressed Osteoclastogenesis. We confirmed that ursolic acid exhibited Osteoclast Differentiation inhibitory activity with an 50% inhibitory concentration (IC50) value of 5.4 ± 0.96 μM. To disclose its mechanism of action, this study first uses polymer-coated magnetic nanobeads to identify potential target proteins. As a result, we identified a nuclear exporter protein named exportin 5 (XPO5). Further studies demonstrated that knockdown of XPO5 significantly blocks Osteoclast Differentiation ( P < 0.01). Expression profiling of mature microRNAs in the cells revealed that downregulation of XPO5 by small interfering RNA or by ursolic acid could downregulate the expression of mature microRNA let-7g-5p during Osteoclast Differentiation ( P < 0.01). Collectively, our findings suggest that ursolic acid inhibits Osteoclast Differentiation through targeting XPO5, which provides further evidence for the healthy function of the tea. This study also provides new insights into the role of XPO5 and its mediated microRNAs in treatment for bone resorption diseases.
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Ursolic Acid Isolated from the Leaves of Loquat (Eriobotrya japonica) Inhibited Osteoclast Differentiation through Targeting Exportin 5
2019Co-Authors: Hui Tan, Chong Zhao, Qinchang Zhu, Yoshinori Katakura, Hiroyuki Tanaka, Koichiro Ohnuki, Kuniyoshi ShimizuAbstract:One of the conventional strategies for treating osteoporosis is to eliminate the multinucleated Osteoclasts that are responsible for bone resorption. Our previous study revealed that ursolic acid, isolated from leaves of loquat that is used as tasty tea in Japan, suppressed Osteoclastogenesis. We confirmed that ursolic acid exhibited Osteoclast Differentiation inhibitory activity with an 50% inhibitory concentration (IC50) value of 5.4 ± 0.96 μM. To disclose its mechanism of action, this study first uses polymer-coated magnetic nanobeads to identify potential target proteins. As a result, we identified a nuclear exporter protein named exportin 5 (XPO5). Further studies demonstrated that knockdown of XPO5 significantly blocks Osteoclast Differentiation (P < 0.01). Expression profiling of mature microRNAs in the cells revealed that downregulation of XPO5 by small interfering RNA or by ursolic acid could downregulate the expression of mature microRNA let-7g-5p during Osteoclast Differentiation (P < 0.01). Collectively, our findings suggest that ursolic acid inhibits Osteoclast Differentiation through targeting XPO5, which provides further evidence for the healthy function of the tea. This study also provides new insights into the role of XPO5 and its mediated microRNAs in treatment for bone resorption diseases
R Mentaverri - One of the best experts on this subject based on the ideXlab platform.
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the calcium sensing receptor is directly involved in both Osteoclast Differentiation and apoptosis
The FASEB Journal, 2006Co-Authors: R Mentaverri, Shozo Yano, Naibedya Chattopadhyay, Laurent Petit, Olga Kifor, S Kamel, Ernest F Terwilliger, Michel BrazierAbstract:Intracellular transduction pathways that are dependent on activation of the CaR by Cao2+ have been studied extensively in parathyroid and other cell types, and include cytosolic calcium, phospholipases C, A2, and D, protein kinase C isoforms and the cAMP/protein kinase A system. In this study, using bone marrow cells isolated from CaR−/− mice as well as DN-CaR-transfected RAW 264.7 cells, we provide evidence that expression of the CaR plays an important role in Osteoclast Differentiation. We also establish that activation of the CaR and resultant stimulation of PLC are involved in high Cao2+-induced apoptosis of mature rabbit Osteoclasts. Similar to RANKL, Cao2+ (20 mM) appeared to trigger rapid and significant nuclear translocation of NF-κB in a CaR- and PLC-dependent manner. In summary, our data suggest that stimulation of the CaR may play a pivotal role in the control of both Osteoclast Differentiation and apoptosis in the systems studied here through a signaling pathway involving activation of the CaR...
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the calcium sensing receptor is directly involved in both Osteoclast Differentiation and apoptosis
The FASEB Journal, 2006Co-Authors: R Mentaverri, Shozo Yano, Naibedya Chattopadhyay, Laurent Petit, Olga Kifor, S Kamel, Ernest F Terwilliger, Michel BrazierAbstract:Intracellular transduction pathways that are dependent on activation of the CaR by Ca(o)2+ have been studied extensively in parathyroid and other cell types, and include cytosolic calcium, phospholipases C, A2, and D, protein kinase C isoforms and the cAMP/protein kinase A system. In this study, using bone marrow cells isolated from CaR-/- mice as well as DN-CaR-transfected RAW 264.7 cells, we provide evidence that expression of the CaR plays an important role in Osteoclast Differentiation. We also establish that activation of the CaR and resultant stimulation of PLC are involved in high Ca(o)2+-induced apoptosis of mature rabbit Osteoclasts. Similar to RANKL, Ca(o)2+ (20 mM) appeared to trigger rapid and significant nuclear translocation of NF-kappaB in a CaR- and PLC-dependent manner. In summary, our data suggest that stimulation of the CaR may play a pivotal role in the control of both Osteoclast Differentiation and apoptosis in the systems studied here through a signaling pathway involving activation of the CaR, phospholipase C, and NF-kappaB.
Yi-ying Wu - One of the best experts on this subject based on the ideXlab platform.
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Radix Paeoniae Rubra stimulates Osteoclast Differentiation by activation of the NF-κB and mitogen-activated protein kinase pathways
BMC Complementary and Alternative Medicine, 2018Co-Authors: Huey-en Tzeng, Chun-hao Tsai, Tin-yun Ho, Chin-tung Hsieh, Shen-chieh Chou, Gregory J. Tsay, Po-hao Huang, Yi-ying WuAbstract:Background Radix Paeoniae Rubra (RPR), a traditional Chinese herb, has anti-inflammatory and immuno-regulatory properties. This study explored the effects of RPR on stimulation of Osteoclast Differentiation in RAW264.7 cells and peripheral blood mononuclear cells (PBMC)s. Methods The mature Osteoclasts were measured by bone resorption assays and TRAP staining. JNK, ERK, p38 and NF-κB inhibitors were used applied in order to verify their contribution in RPR-induced Osteoclast Differentiation. The NF-κB and MAPK pathways were evaluated by western blotting, RT-PCR and luciferase assay. Results RPR induced Osteoclast Differentiation in a dose-dependent manner and induced the resorption activity of Osteoclasts Differentiation of RAW264.7 cells and PBMCs. Western blotting showed that RPR treatment induced phosphorylation of JNK, ERK, and p38 in RAW 264.7 cells. Treatment of JNK, ERK, and p38 MAP kinase inhibitors verified the contribution of JNK, ERK and p38. RPR treatment induced c-Fos and NFATc1 protein expression; NF-κB inhibitor treatment and luciferase assay verified the contribution of the NF-κB pathway. Conclusions This study demonstrated the interesting effect, in which RPR stimulated Osteoclast Differentiation in murine RAW264.7 cells and human monocytes.
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radix paeoniae rubra stimulates Osteoclast Differentiation by activation of the nf κb and mitogen activated protein kinase pathways
BMC Complementary and Alternative Medicine, 2018Co-Authors: Huey-en Tzeng, Chun-hao Tsai, Tin-yun Ho, Chin-tung Hsieh, Shen-chieh Chou, Gregory J. Tsay, Po-hao Huang, Yi-ying WuAbstract:Radix Paeoniae Rubra (RPR), a traditional Chinese herb, has anti-inflammatory and immuno-regulatory properties. This study explored the effects of RPR on stimulation of Osteoclast Differentiation in RAW264.7 cells and peripheral blood mononuclear cells (PBMC)s. The mature Osteoclasts were measured by bone resorption assays and TRAP staining. JNK, ERK, p38 and NF-κB inhibitors were used applied in order to verify their contribution in RPR-induced Osteoclast Differentiation. The NF-κB and MAPK pathways were evaluated by western blotting, RT-PCR and luciferase assay. RPR induced Osteoclast Differentiation in a dose-dependent manner and induced the resorption activity of Osteoclasts Differentiation of RAW264.7 cells and PBMCs. Western blotting showed that RPR treatment induced phosphorylation of JNK, ERK, and p38 in RAW 264.7 cells. Treatment of JNK, ERK, and p38 MAP kinase inhibitors verified the contribution of JNK, ERK and p38. RPR treatment induced c-Fos and NFATc1 protein expression; NF-κB inhibitor treatment and luciferase assay verified the contribution of the NF-κB pathway. This study demonstrated the interesting effect, in which RPR stimulated Osteoclast Differentiation in murine RAW264.7 cells and human monocytes.
Michel Brazier - One of the best experts on this subject based on the ideXlab platform.
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the calcium sensing receptor is directly involved in both Osteoclast Differentiation and apoptosis
The FASEB Journal, 2006Co-Authors: R Mentaverri, Shozo Yano, Naibedya Chattopadhyay, Laurent Petit, Olga Kifor, S Kamel, Ernest F Terwilliger, Michel BrazierAbstract:Intracellular transduction pathways that are dependent on activation of the CaR by Cao2+ have been studied extensively in parathyroid and other cell types, and include cytosolic calcium, phospholipases C, A2, and D, protein kinase C isoforms and the cAMP/protein kinase A system. In this study, using bone marrow cells isolated from CaR−/− mice as well as DN-CaR-transfected RAW 264.7 cells, we provide evidence that expression of the CaR plays an important role in Osteoclast Differentiation. We also establish that activation of the CaR and resultant stimulation of PLC are involved in high Cao2+-induced apoptosis of mature rabbit Osteoclasts. Similar to RANKL, Cao2+ (20 mM) appeared to trigger rapid and significant nuclear translocation of NF-κB in a CaR- and PLC-dependent manner. In summary, our data suggest that stimulation of the CaR may play a pivotal role in the control of both Osteoclast Differentiation and apoptosis in the systems studied here through a signaling pathway involving activation of the CaR...
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the calcium sensing receptor is directly involved in both Osteoclast Differentiation and apoptosis
The FASEB Journal, 2006Co-Authors: R Mentaverri, Shozo Yano, Naibedya Chattopadhyay, Laurent Petit, Olga Kifor, S Kamel, Ernest F Terwilliger, Michel BrazierAbstract:Intracellular transduction pathways that are dependent on activation of the CaR by Ca(o)2+ have been studied extensively in parathyroid and other cell types, and include cytosolic calcium, phospholipases C, A2, and D, protein kinase C isoforms and the cAMP/protein kinase A system. In this study, using bone marrow cells isolated from CaR-/- mice as well as DN-CaR-transfected RAW 264.7 cells, we provide evidence that expression of the CaR plays an important role in Osteoclast Differentiation. We also establish that activation of the CaR and resultant stimulation of PLC are involved in high Ca(o)2+-induced apoptosis of mature rabbit Osteoclasts. Similar to RANKL, Ca(o)2+ (20 mM) appeared to trigger rapid and significant nuclear translocation of NF-kappaB in a CaR- and PLC-dependent manner. In summary, our data suggest that stimulation of the CaR may play a pivotal role in the control of both Osteoclast Differentiation and apoptosis in the systems studied here through a signaling pathway involving activation of the CaR, phospholipase C, and NF-kappaB.
Hui Tan - One of the best experts on this subject based on the ideXlab platform.
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Ursolic Acid Isolated from the Leaves of Loquat ( Eriobotrya japonica) Inhibited Osteoclast Differentiation through Targeting Exportin 5.
Journal of agricultural and food chemistry, 2019Co-Authors: Hui Tan, Chong Zhao, Qinchang Zhu, Yoshinori Katakura, Hiroyuki Tanaka, Koichiro Ohnuki, Kuniyoshi ShimizuAbstract:One of the conventional strategies for treating osteoporosis is to eliminate the multinucleated Osteoclasts that are responsible for bone resorption. Our previous study revealed that ursolic acid, isolated from leaves of loquat that is used as tasty tea in Japan, suppressed Osteoclastogenesis. We confirmed that ursolic acid exhibited Osteoclast Differentiation inhibitory activity with an 50% inhibitory concentration (IC50) value of 5.4 ± 0.96 μM. To disclose its mechanism of action, this study first uses polymer-coated magnetic nanobeads to identify potential target proteins. As a result, we identified a nuclear exporter protein named exportin 5 (XPO5). Further studies demonstrated that knockdown of XPO5 significantly blocks Osteoclast Differentiation ( P < 0.01). Expression profiling of mature microRNAs in the cells revealed that downregulation of XPO5 by small interfering RNA or by ursolic acid could downregulate the expression of mature microRNA let-7g-5p during Osteoclast Differentiation ( P < 0.01). Collectively, our findings suggest that ursolic acid inhibits Osteoclast Differentiation through targeting XPO5, which provides further evidence for the healthy function of the tea. This study also provides new insights into the role of XPO5 and its mediated microRNAs in treatment for bone resorption diseases.
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Ursolic Acid Isolated from the Leaves of Loquat (Eriobotrya japonica) Inhibited Osteoclast Differentiation through Targeting Exportin 5
2019Co-Authors: Hui Tan, Chong Zhao, Qinchang Zhu, Yoshinori Katakura, Hiroyuki Tanaka, Koichiro Ohnuki, Kuniyoshi ShimizuAbstract:One of the conventional strategies for treating osteoporosis is to eliminate the multinucleated Osteoclasts that are responsible for bone resorption. Our previous study revealed that ursolic acid, isolated from leaves of loquat that is used as tasty tea in Japan, suppressed Osteoclastogenesis. We confirmed that ursolic acid exhibited Osteoclast Differentiation inhibitory activity with an 50% inhibitory concentration (IC50) value of 5.4 ± 0.96 μM. To disclose its mechanism of action, this study first uses polymer-coated magnetic nanobeads to identify potential target proteins. As a result, we identified a nuclear exporter protein named exportin 5 (XPO5). Further studies demonstrated that knockdown of XPO5 significantly blocks Osteoclast Differentiation (P < 0.01). Expression profiling of mature microRNAs in the cells revealed that downregulation of XPO5 by small interfering RNA or by ursolic acid could downregulate the expression of mature microRNA let-7g-5p during Osteoclast Differentiation (P < 0.01). Collectively, our findings suggest that ursolic acid inhibits Osteoclast Differentiation through targeting XPO5, which provides further evidence for the healthy function of the tea. This study also provides new insights into the role of XPO5 and its mediated microRNAs in treatment for bone resorption diseases
