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Prachi Masih - One of the best experts on this subject based on the ideXlab platform.
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genetic divergence in natural populations of bronze featherback notopterus notopterus Osteoglossiformes notopteridae from five indian rivers analyzed through mtdna atpase6 8 regions
Meta Gene, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi Masih, Braj Kishor Gupta, Rahasya Mani MishraAbstract:The present study characterized 842 bp fragment of mitochondrial ATP synthase 6 and 8 (ATPase6/8) genes in Notopterus notopterus. In all, 97 samples of N. notopterus were collected from five distant rivers; viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi representing 4 river basins in India. The analysis of variation revealed presence of 23 haplotypes in ATPase6/8 gene with haplotype diversity (Hd) of 0.899 and nucleotide diversity (π) of 0.00336. The within population variation which was 41.78% of the total variation of 58.22% was found among population. The Fst value of 0.582 (P < 0.05) of the total population was found significant. The results concluded that the polymorphism in ATPase6/8 gene is a potential marker that is important for determining genetic divergence of wild N. notopterus populations. The findings reveal common ancestry of mahanadi population with the populations in rivers of Indo-Gangetic region. However, long evolutionary isolation must be responsible for the high genetic divergence between N. notopterus in Mahanadi and other regions.
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characterization of polymorphic microsatellite markers and genetic diversity in wild bronze featherback notopterus notopterus pallas 1769
Molecular Biology Reports, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Rajesh Kumar, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rama S Sah, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi MasihAbstract:Six polymorphic microsatellite DNA loci were identified in the primitive fish, bronze featherback, Notopterus notopterus for the first time and demonstrated significant population genetic structure. Out of the six primers, one primer (NN90) was specific to N. notopterus (microsatellite sequence within the RAG1 gene) and five primers were product of successful cross-species amplification. Sixty-four primers available from 3 fish species of order Osteoglossiformes and families Notopteridae and Osteoglossidae were tested to amplify homologous microsatellite loci in N. notopterus. Fifteen primer pairs exhibited successful cross-priming PCR product. However, polymorphism was detected only at five loci. To assess the significance of these six loci (including NN90) in population genetic study, 215 samples of N. notopterus from five rivers, viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi were analyzed. The five sample sets displayed different diversity levels and observed heterozygosity ranged from 0.6036 to 0.7373. Significant genotype heterogeneity (P < 0.0001) and high FST (0.2205) over all loci indicated that the samples are not drawn from the same genepool. The identified microsatellite loci are promising for use in fine-scale population structure analysis of N. notopterus.
Arti Gupta - One of the best experts on this subject based on the ideXlab platform.
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genetic divergence in natural populations of bronze featherback notopterus notopterus Osteoglossiformes notopteridae from five indian rivers analyzed through mtdna atpase6 8 regions
Meta Gene, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi Masih, Braj Kishor Gupta, Rahasya Mani MishraAbstract:The present study characterized 842 bp fragment of mitochondrial ATP synthase 6 and 8 (ATPase6/8) genes in Notopterus notopterus. In all, 97 samples of N. notopterus were collected from five distant rivers; viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi representing 4 river basins in India. The analysis of variation revealed presence of 23 haplotypes in ATPase6/8 gene with haplotype diversity (Hd) of 0.899 and nucleotide diversity (π) of 0.00336. The within population variation which was 41.78% of the total variation of 58.22% was found among population. The Fst value of 0.582 (P < 0.05) of the total population was found significant. The results concluded that the polymorphism in ATPase6/8 gene is a potential marker that is important for determining genetic divergence of wild N. notopterus populations. The findings reveal common ancestry of mahanadi population with the populations in rivers of Indo-Gangetic region. However, long evolutionary isolation must be responsible for the high genetic divergence between N. notopterus in Mahanadi and other regions.
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characterization of polymorphic microsatellite markers and genetic diversity in wild bronze featherback notopterus notopterus pallas 1769
Molecular Biology Reports, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Rajesh Kumar, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rama S Sah, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi MasihAbstract:Six polymorphic microsatellite DNA loci were identified in the primitive fish, bronze featherback, Notopterus notopterus for the first time and demonstrated significant population genetic structure. Out of the six primers, one primer (NN90) was specific to N. notopterus (microsatellite sequence within the RAG1 gene) and five primers were product of successful cross-species amplification. Sixty-four primers available from 3 fish species of order Osteoglossiformes and families Notopteridae and Osteoglossidae were tested to amplify homologous microsatellite loci in N. notopterus. Fifteen primer pairs exhibited successful cross-priming PCR product. However, polymorphism was detected only at five loci. To assess the significance of these six loci (including NN90) in population genetic study, 215 samples of N. notopterus from five rivers, viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi were analyzed. The five sample sets displayed different diversity levels and observed heterozygosity ranged from 0.6036 to 0.7373. Significant genotype heterogeneity (P < 0.0001) and high FST (0.2205) over all loci indicated that the samples are not drawn from the same genepool. The identified microsatellite loci are promising for use in fine-scale population structure analysis of N. notopterus.
Rajeev K. Singh - One of the best experts on this subject based on the ideXlab platform.
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genetic divergence in natural populations of bronze featherback notopterus notopterus Osteoglossiformes notopteridae from five indian rivers analyzed through mtdna atpase6 8 regions
Meta Gene, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi Masih, Braj Kishor Gupta, Rahasya Mani MishraAbstract:The present study characterized 842 bp fragment of mitochondrial ATP synthase 6 and 8 (ATPase6/8) genes in Notopterus notopterus. In all, 97 samples of N. notopterus were collected from five distant rivers; viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi representing 4 river basins in India. The analysis of variation revealed presence of 23 haplotypes in ATPase6/8 gene with haplotype diversity (Hd) of 0.899 and nucleotide diversity (π) of 0.00336. The within population variation which was 41.78% of the total variation of 58.22% was found among population. The Fst value of 0.582 (P < 0.05) of the total population was found significant. The results concluded that the polymorphism in ATPase6/8 gene is a potential marker that is important for determining genetic divergence of wild N. notopterus populations. The findings reveal common ancestry of mahanadi population with the populations in rivers of Indo-Gangetic region. However, long evolutionary isolation must be responsible for the high genetic divergence between N. notopterus in Mahanadi and other regions.
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characterization of polymorphic microsatellite markers and genetic diversity in wild bronze featherback notopterus notopterus pallas 1769
Molecular Biology Reports, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Rajesh Kumar, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rama S Sah, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi MasihAbstract:Six polymorphic microsatellite DNA loci were identified in the primitive fish, bronze featherback, Notopterus notopterus for the first time and demonstrated significant population genetic structure. Out of the six primers, one primer (NN90) was specific to N. notopterus (microsatellite sequence within the RAG1 gene) and five primers were product of successful cross-species amplification. Sixty-four primers available from 3 fish species of order Osteoglossiformes and families Notopteridae and Osteoglossidae were tested to amplify homologous microsatellite loci in N. notopterus. Fifteen primer pairs exhibited successful cross-priming PCR product. However, polymorphism was detected only at five loci. To assess the significance of these six loci (including NN90) in population genetic study, 215 samples of N. notopterus from five rivers, viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi were analyzed. The five sample sets displayed different diversity levels and observed heterozygosity ranged from 0.6036 to 0.7373. Significant genotype heterogeneity (P < 0.0001) and high FST (0.2205) over all loci indicated that the samples are not drawn from the same genepool. The identified microsatellite loci are promising for use in fine-scale population structure analysis of N. notopterus.
Arvind Kumar Dwivedi - One of the best experts on this subject based on the ideXlab platform.
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genetic divergence in natural populations of bronze featherback notopterus notopterus Osteoglossiformes notopteridae from five indian rivers analyzed through mtdna atpase6 8 regions
Meta Gene, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi Masih, Braj Kishor Gupta, Rahasya Mani MishraAbstract:The present study characterized 842 bp fragment of mitochondrial ATP synthase 6 and 8 (ATPase6/8) genes in Notopterus notopterus. In all, 97 samples of N. notopterus were collected from five distant rivers; viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi representing 4 river basins in India. The analysis of variation revealed presence of 23 haplotypes in ATPase6/8 gene with haplotype diversity (Hd) of 0.899 and nucleotide diversity (π) of 0.00336. The within population variation which was 41.78% of the total variation of 58.22% was found among population. The Fst value of 0.582 (P < 0.05) of the total population was found significant. The results concluded that the polymorphism in ATPase6/8 gene is a potential marker that is important for determining genetic divergence of wild N. notopterus populations. The findings reveal common ancestry of mahanadi population with the populations in rivers of Indo-Gangetic region. However, long evolutionary isolation must be responsible for the high genetic divergence between N. notopterus in Mahanadi and other regions.
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characterization of polymorphic microsatellite markers and genetic diversity in wild bronze featherback notopterus notopterus pallas 1769
Molecular Biology Reports, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Rajesh Kumar, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rama S Sah, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi MasihAbstract:Six polymorphic microsatellite DNA loci were identified in the primitive fish, bronze featherback, Notopterus notopterus for the first time and demonstrated significant population genetic structure. Out of the six primers, one primer (NN90) was specific to N. notopterus (microsatellite sequence within the RAG1 gene) and five primers were product of successful cross-species amplification. Sixty-four primers available from 3 fish species of order Osteoglossiformes and families Notopteridae and Osteoglossidae were tested to amplify homologous microsatellite loci in N. notopterus. Fifteen primer pairs exhibited successful cross-priming PCR product. However, polymorphism was detected only at five loci. To assess the significance of these six loci (including NN90) in population genetic study, 215 samples of N. notopterus from five rivers, viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi were analyzed. The five sample sets displayed different diversity levels and observed heterozygosity ranged from 0.6036 to 0.7373. Significant genotype heterogeneity (P < 0.0001) and high FST (0.2205) over all loci indicated that the samples are not drawn from the same genepool. The identified microsatellite loci are promising for use in fine-scale population structure analysis of N. notopterus.
Rupesh K Luhariya - One of the best experts on this subject based on the ideXlab platform.
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genetic divergence in natural populations of bronze featherback notopterus notopterus Osteoglossiformes notopteridae from five indian rivers analyzed through mtdna atpase6 8 regions
Meta Gene, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi Masih, Braj Kishor Gupta, Rahasya Mani MishraAbstract:The present study characterized 842 bp fragment of mitochondrial ATP synthase 6 and 8 (ATPase6/8) genes in Notopterus notopterus. In all, 97 samples of N. notopterus were collected from five distant rivers; viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi representing 4 river basins in India. The analysis of variation revealed presence of 23 haplotypes in ATPase6/8 gene with haplotype diversity (Hd) of 0.899 and nucleotide diversity (π) of 0.00336. The within population variation which was 41.78% of the total variation of 58.22% was found among population. The Fst value of 0.582 (P < 0.05) of the total population was found significant. The results concluded that the polymorphism in ATPase6/8 gene is a potential marker that is important for determining genetic divergence of wild N. notopterus populations. The findings reveal common ancestry of mahanadi population with the populations in rivers of Indo-Gangetic region. However, long evolutionary isolation must be responsible for the high genetic divergence between N. notopterus in Mahanadi and other regions.
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characterization of polymorphic microsatellite markers and genetic diversity in wild bronze featherback notopterus notopterus pallas 1769
Molecular Biology Reports, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Rajesh Kumar, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rama S Sah, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi MasihAbstract:Six polymorphic microsatellite DNA loci were identified in the primitive fish, bronze featherback, Notopterus notopterus for the first time and demonstrated significant population genetic structure. Out of the six primers, one primer (NN90) was specific to N. notopterus (microsatellite sequence within the RAG1 gene) and five primers were product of successful cross-species amplification. Sixty-four primers available from 3 fish species of order Osteoglossiformes and families Notopteridae and Osteoglossidae were tested to amplify homologous microsatellite loci in N. notopterus. Fifteen primer pairs exhibited successful cross-priming PCR product. However, polymorphism was detected only at five loci. To assess the significance of these six loci (including NN90) in population genetic study, 215 samples of N. notopterus from five rivers, viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi were analyzed. The five sample sets displayed different diversity levels and observed heterozygosity ranged from 0.6036 to 0.7373. Significant genotype heterogeneity (P < 0.0001) and high FST (0.2205) over all loci indicated that the samples are not drawn from the same genepool. The identified microsatellite loci are promising for use in fine-scale population structure analysis of N. notopterus.
