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Cienfuegos Alayo, Jhoselin Mariset - One of the best experts on this subject based on the ideXlab platform.
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Presencia de Malassezia pachydermatis en Canis familiaris diagnosticados clínicamente con Otitis Externa en el distrito de Nuevo Chimbote, región Ancash - Perú 2016
Universidad Privada Antenor Orrego - UPAO, 2017Co-Authors: Cienfuegos Alayo, Jhoselin MarisetAbstract:La Otitis canina Externa es una inflamación del conducto auditivo externo, que se caracteriza por eritema, aumento de la descarga o descamación del epitelio, acompañado de dolor y/o irritación; entre uno de los agentes responsables de esta afección se tiene a Malassezia pachydermatis. El presente trabajo de estudio, se realizó para determinar la presencia de Malassezia pachydermatis en Canis familiaris, diagnosticados clínicamente con Otitis Externa, en el distrito de Nuevo Chimbote, región Ancash - Perú - 2016. Se utilizaron 53 Canis familiraris, como material biológico, los cuales fueron de diferente raza, edad, sexo. Se empleó como medio de diagnóstico, la citología por extendido directo, demostrándose la presencia de la levadura en un 74% (N=39/53) en Canis familiaris con Otitis Externa y en un 26% (N=14/53) de Otitis canina Externa debido a otras causas.External canine Otitis is an inflammation of the External auditory canal, characterized by erythema, increased discharge or scaling of the epithelium, accompanied by pain and / or irritation; One of the agents responsible for this condition has Malassezia pachydermatis. The present study was carried out to determine the presence of Malassezia pachydermatis in Canis familiaris, clinically diagnosed with Otitis Externa, in the district of Nuevo Chimbote, Ancash region - Peru - 2016. We used 53 Canis familiraris, as biological material, which Were of different race, age, sex. Direct cytology was used as a diagnostic medium, with the presence of yeast in 74% (N = 39/53) in Canis familiaris with External Otitis and 26% (N = 14/53) of Otitis Canine due to other causes.Tesi
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Presencia de malassezia pachydermatis en canis familiaris diagnosticados clinicamente con Otitis Externa en el distrito de nuevo chimbote, región ancash - peru 2016
'Universidad Privada Antenor Orrego', 2017Co-Authors: Cienfuegos Alayo, Jhoselin MarisetAbstract:External canine Otitis is an inflammation of the External auditory canal, characterized by erythema, increased discharge or scaling of the epithelium, accompanied by pain and / or irritation; One of the agents responsible for this condition has Malassezia pachydermatis. The present study was carried out to determine the presence of Malassezia pachydermatis in Canis familiaris, clinically diagnosed with Otitis Externa, in the district of Nuevo Chimbote, Ancash region - Peru - 2016. We used 53 Canis familiraris, as biological material, which Were of different race, age, sex. Direct cytology was used as a diagnostic medium, with the presence of yeast in 74% (N = 39/53) in Canis familiaris with External Otitis and 26% (N = 14/53) of Otitis Canine due to other causes.La Otitis canina Externa es una inflamación del conducto auditivo externo, que se caracteriza por eritema, aumento de la descarga o descamación del epitelio, acompañado de dolor y/o irritación; entre uno de los agentes responsables de esta afección se tiene a Malassezia pachydermatis. El presente trabajo de estudio, se realizó para determinar la presencia de Malassezia pachydermatis en Canis familiaris, diagnosticados clínicamente con Otitis Externa, en el distrito de Nuevo Chimbote, región Ancash - Perú - 2016. Se utilizaron 53 Canis familiraris, como material biológico, los cuales fueron de diferente raza, edad, sexo. Se empleó como medio de diagnóstico, la citología por extendido directo, demostrándose la presencia de la levadura en un 74% (N=39/53) en Canis familiaris con Otitis Externa y en un 26% (N=14/53) de Otitis canina Externa debido a otras causas
G Szigeti - One of the best experts on this subject based on the ideXlab platform.
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characteristics of malassezia pachydermatis strains isolated from canine Otitis Externa
Mycoses, 1996Co-Authors: Gabriella Kiss, Sz Radvanyi, G SzigetiAbstract:The morphological, cultural and biochemical characteristics of 80 M. pachydermatis strains isolated from cases of canine Otitis Externa were studied. Microscopically, the strains could be subdivided into two phenotypes. All M. pachydermatis strains grew well on Sabouraud glucose, yeast morphology and modified malt extract agar, but formed two distinct colony types. All strains were characterized by no fermentation. Assimilation of glucose, mannitol (42 strains), sorbitol (40 strains) and peptone was observed, but no ethanol assimilation. Urease and catalase tests were positive, while indole and acetoin production was not detected. All strains showed proteinase, caseinase, lecithinase and peroxidase positivity but to varying extents. Esterase activity was observed for all Malassezia strains when using Tween 20, 40 and 60, whereas Tween 80 was hydrolysed by only 42 strains. No coagulase or haemagglutinating activities were detected. When compared for satellite phenomenon and vitamin requirements, some Malassezia strains could not grow in the absence of nicotinic acid but grew well in the presence of staphylococci. In susceptibility tests, all strains showed the highest susceptibility to ketoconazole. On the basis of the biochemical differences, M. pachydermatis seems to be a heterogeneous species and can be divided into two groups.
J W R Capper - One of the best experts on this subject based on the ideXlab platform.
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External auditory canal ph in chronic Otitis Externa
Clinical Otolaryngology, 2003Co-Authors: Martinez P Devesa, C M Willis, J W R CapperAbstract:External auditory canal pH in chronic Otitis Externa Several risk factors have been postulated to play a part in the progression of acute into chronic Otitis Externa, including changes towards alkalinity of the skin pH of the External auditory canal. These changes have been previously reported to occur in the acute stage, and their persistence may predispose to a chronic status of this condition. This prospective control study was designed to look at this possible relationship in more depth, by comparing the External auditory canal pH of individuals with chronic Otitis Externa, but with no current exacerbation, with an age-/sex-matched control group. Analysis of the data revealed a significantly higher External auditory canal pH in the chronic Otitis Externa group (P < 0.004) when compared with the controls, with no concomitant difference in body skin pH. Within this chronic Otitis Externa cohort, a statistically significant correlation was found between External auditory canal pH and the severity of the condition, as assessed using a multiparameter scoring system (r = 0.562; P < 0.02). Importantly, the pH was not related to the length of time since the last exacerbation. There was a significant age relationship present within our sample. Younger patients displayed more severe chronic Otitis Externa(r = −0.813; P < 0.001), with correspondingly higher External auditory canal pH values (r = −0.550; P < 0.02). The results suggest that alkaline ear canal skin is a local risk factor for the progression into chronic Otitis Externa, occurring, in this study, with greater severity in younger individuals.
Adam Tylicki - One of the best experts on this subject based on the ideXlab platform.
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comparison of lipid profiles of malassezia pachydermatis strains isolated from dogs with Otitis Externa and without clinical symptoms of disease
Mycoses, 2016Co-Authors: Urszula Czyzewska, Magdalena Siemieniuk, Aleksandra Pyrkowska, Aneta Nowakiewicz, Malgorzata Bieganska, Iwona Dabrowska, Marek Bartoszewicz, Pawel Dobrzyn, Adam TylickiAbstract:Malassezia pachydermatis can cause infections of the skin and mucous membranes, especially in animals. It becomes a problem also in medicine. It is considered that metabolic disorders as well as hormonal and immunological status of the host promote diseases caused by M. pachydermatis. Here we consider whether specific features of fungi could also favour infections. We checked whether there are differences in lipid profiles between strains obtained from dogs with Otitis Externa and strains obtained from healthy dogs. Lipid profiles were determined using thin layer chromatography and gas chromatography-mass spectrometry. All analyses were carried out on 32 strains derived from dogs with Otitis Externa and 31 strains isolated from dogs without symptoms of disease. The results show that strains isolated from dogs without symptoms of Otitis Externa are characterised by a higher content of fatty acids. They contain significantly more behenic and lignoceric acids on medium without addition of lipids, and more oleic acid and total monounsaturated fatty acids on medium with lipids supplementation. These strains have also a higher content of esters of ergosterol and triglycerides. Data obtained show differences which may be specific features of M. pachydermatis-specific strains related to the ability of infection, which could be not directly related of the host condition.
Gabriella Kiss - One of the best experts on this subject based on the ideXlab platform.
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characteristics of malassezia pachydermatis strains isolated from canine Otitis Externa
Mycoses, 1996Co-Authors: Gabriella Kiss, Sz Radvanyi, G SzigetiAbstract:The morphological, cultural and biochemical characteristics of 80 M. pachydermatis strains isolated from cases of canine Otitis Externa were studied. Microscopically, the strains could be subdivided into two phenotypes. All M. pachydermatis strains grew well on Sabouraud glucose, yeast morphology and modified malt extract agar, but formed two distinct colony types. All strains were characterized by no fermentation. Assimilation of glucose, mannitol (42 strains), sorbitol (40 strains) and peptone was observed, but no ethanol assimilation. Urease and catalase tests were positive, while indole and acetoin production was not detected. All strains showed proteinase, caseinase, lecithinase and peroxidase positivity but to varying extents. Esterase activity was observed for all Malassezia strains when using Tween 20, 40 and 60, whereas Tween 80 was hydrolysed by only 42 strains. No coagulase or haemagglutinating activities were detected. When compared for satellite phenomenon and vitamin requirements, some Malassezia strains could not grow in the absence of nicotinic acid but grew well in the presence of staphylococci. In susceptibility tests, all strains showed the highest susceptibility to ketoconazole. On the basis of the biochemical differences, M. pachydermatis seems to be a heterogeneous species and can be divided into two groups.
