The Experts below are selected from a list of 35363832 Experts worldwide ranked by ideXlab platform
Jean E Rivier - One of the best experts on this subject based on the ideXlab platform.
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three forms of gonadotropin releasing hormone including a novel form in a basal salmonid coregonus clupeaformis
Biology of Reproduction, 2002Co-Authors: Bruce A Adams, Elaine D Vickers, Carol M Warby, Minkyu Park, Wolfgang Fischer, Grey A Craig, Jean E RivierAbstract:Multiple forms of GnRH within individual brains may have different functions. However, some vertebrates such as salmonids continue to reproduce even though they have lost or do not express 1 of the 3 forms of GnRH found in most other teleosts. We examined a basal salmonid, lake whitefish, to determine the mechanism by which a reduction in the number of GnRH forms occurs. We identified for the first time 3 distinct GnRHs in a salmonid. One form is novel and is designated whitefish GnRH. The primary structure is pGlu-His-Trp-Ser-TyrGly-Met-Asn-Pro-Gly-NH2. HPLC and RIA were used for purification followed by Edman degradation for sequence determination. Mass spectroscopy was used to confirm the sequence and amidation of the peptide. The other 2 forms, salmon GnRH and chicken GnRH-II, are identical to the 2 forms found in salmon, which evolved later than whitefish. Synthetic whitefish GnRH is biologically active, as it increased mRNA expression of growth hormone and the a-subunit for LH and thyroid-stimulating hormone in dispersed fish pituitary cells. Our data support the hypothesis that the ancestral salmonid had a third GnRH form when the genome doubled (tetraploidization), but the third form was lost later in some salmonids due to chromosomal rearrangements. We suggest that the salmon GnRH form compensated for the loss of the third form.
Koen Dewettinck - One of the best experts on this subject based on the ideXlab platform.
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food grade monoglyceride oil foams the effect of tempering on foamability foam stability and rheological properties
Food & Function, 2018Co-Authors: Robbe Heymans, Iris Tavernier, Sabine Danthine, Tom Rimaux, Paul Van Der Meeren, Koen DewettinckAbstract:Foams with a continuous oil phase may be stabilized using crystalline particles. Those systems are compelling because of their potential in edible oil structuring, modifying sensorial properties and creating healthier food products. This study aimed to relate oleogel (unwhipped state) properties to oil foam (whipped state) properties using a monoglyceride-sunflower oil model system. The properties of crystal–oil mixtures were influenced by time and temperature during preparation and storage. Therefore, oleogels were prepared using different tempering protocols and their resulting microstructure was investigated with rheology, differential scanning calorimetry and X-ray diffraction. The corresponding oil foams were characterized in terms of foamability and foam stability. The properties of both systems were studied immediately after preparation as well as after 4 weeks of storage. We demonstrated that there is a large influence of the time-temperature history on the foam properties. Partially crystallized mixtures were shown to form weaker structures which capture more air because of their lower viscosity and as crystallization would preferentially take place at the interface. They were characterized by larger bubbles and were less stable and firm. It is proposed that their rheological properties are mainly dominated by interfacial contributions. Fully crystallized and stored monoglyceride–oil mixtures were seen to form stronger gel networks which included less air, contained smaller air bubbles and were stable during storage. It is hypothesized that these samples also included an important bulk gelation contribution.
Bruce A Adams - One of the best experts on this subject based on the ideXlab platform.
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cloning and localization of three forms of gonadotropin releasing hormone including the novel whitefish form in a salmonid coregonus clupeaformis
Biology of Reproduction, 2004Co-Authors: Elaine D Vickers, Frederic Laberge, Bruce A Adams, Toshiaki J Hara, Nancy M SherwoodAbstract:Cells containing different GnRH peptides currently are thought to have distinct locations and functions in the brain. Lake whitefish is the first salmonid species to have three forms of GnRH peptide in contrast to later-evolving salmonids (salmon and trout) in which only two forms have been identified. Our objective was to isolate the cDNAs that code for these transcripts and to localize the transcripts for the three forms of GnRH in adult lake whitefish brain. Also, we provide phylogenetic analysis of these three whitefish genes based on their preprohormone sequence. From whitefish we isolated cDNAs encoding chicken (c)GnRH-II, salmon (s)GnRH, and the novel whitefish (wf)GnRH. The three cDNAs each encode only one GnRH and are placed in separate groups with phylogenetic analysis. A combination of in situ hybridization and immunocytochemistry with two antisera revealed neurons that expressed protein and/or mRNA for cGnRH-II in the midbrain and hindbrain; sGnRH in the olfactory nerve and bulb, ventral telencephalon, and preoptic area; and wfGnRH in the same latter two brain regions and the hypothalamus. Thus, in the anterior brain, cells containing sGnRH and wfGnRH were in the same brain areas but not at identical locations in the ventral telencephalon and preoptic area. Based on our results, we speculate that both sGnRH and wfGnRH have gonadotropin-releasing roles in the lake whitefish brain. central nervous system, gonadotropin-releasing hormone, neuroendocrinology, neuropeptides
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three forms of gonadotropin releasing hormone including a novel form in a basal salmonid coregonus clupeaformis
Biology of Reproduction, 2002Co-Authors: Bruce A Adams, Elaine D Vickers, Carol M Warby, Minkyu Park, Wolfgang Fischer, Grey A Craig, Jean E RivierAbstract:Multiple forms of GnRH within individual brains may have different functions. However, some vertebrates such as salmonids continue to reproduce even though they have lost or do not express 1 of the 3 forms of GnRH found in most other teleosts. We examined a basal salmonid, lake whitefish, to determine the mechanism by which a reduction in the number of GnRH forms occurs. We identified for the first time 3 distinct GnRHs in a salmonid. One form is novel and is designated whitefish GnRH. The primary structure is pGlu-His-Trp-Ser-TyrGly-Met-Asn-Pro-Gly-NH2. HPLC and RIA were used for purification followed by Edman degradation for sequence determination. Mass spectroscopy was used to confirm the sequence and amidation of the peptide. The other 2 forms, salmon GnRH and chicken GnRH-II, are identical to the 2 forms found in salmon, which evolved later than whitefish. Synthetic whitefish GnRH is biologically active, as it increased mRNA expression of growth hormone and the a-subunit for LH and thyroid-stimulating hormone in dispersed fish pituitary cells. Our data support the hypothesis that the ancestral salmonid had a third GnRH form when the genome doubled (tetraploidization), but the third form was lost later in some salmonids due to chromosomal rearrangements. We suggest that the salmon GnRH form compensated for the loss of the third form.
Wolfgang Fischer - One of the best experts on this subject based on the ideXlab platform.
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three forms of gonadotropin releasing hormone including a novel form in a basal salmonid coregonus clupeaformis
Biology of Reproduction, 2002Co-Authors: Bruce A Adams, Elaine D Vickers, Carol M Warby, Minkyu Park, Wolfgang Fischer, Grey A Craig, Jean E RivierAbstract:Multiple forms of GnRH within individual brains may have different functions. However, some vertebrates such as salmonids continue to reproduce even though they have lost or do not express 1 of the 3 forms of GnRH found in most other teleosts. We examined a basal salmonid, lake whitefish, to determine the mechanism by which a reduction in the number of GnRH forms occurs. We identified for the first time 3 distinct GnRHs in a salmonid. One form is novel and is designated whitefish GnRH. The primary structure is pGlu-His-Trp-Ser-TyrGly-Met-Asn-Pro-Gly-NH2. HPLC and RIA were used for purification followed by Edman degradation for sequence determination. Mass spectroscopy was used to confirm the sequence and amidation of the peptide. The other 2 forms, salmon GnRH and chicken GnRH-II, are identical to the 2 forms found in salmon, which evolved later than whitefish. Synthetic whitefish GnRH is biologically active, as it increased mRNA expression of growth hormone and the a-subunit for LH and thyroid-stimulating hormone in dispersed fish pituitary cells. Our data support the hypothesis that the ancestral salmonid had a third GnRH form when the genome doubled (tetraploidization), but the third form was lost later in some salmonids due to chromosomal rearrangements. We suggest that the salmon GnRH form compensated for the loss of the third form.
Grey A Craig - One of the best experts on this subject based on the ideXlab platform.
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three forms of gonadotropin releasing hormone including a novel form in a basal salmonid coregonus clupeaformis
Biology of Reproduction, 2002Co-Authors: Bruce A Adams, Elaine D Vickers, Carol M Warby, Minkyu Park, Wolfgang Fischer, Grey A Craig, Jean E RivierAbstract:Multiple forms of GnRH within individual brains may have different functions. However, some vertebrates such as salmonids continue to reproduce even though they have lost or do not express 1 of the 3 forms of GnRH found in most other teleosts. We examined a basal salmonid, lake whitefish, to determine the mechanism by which a reduction in the number of GnRH forms occurs. We identified for the first time 3 distinct GnRHs in a salmonid. One form is novel and is designated whitefish GnRH. The primary structure is pGlu-His-Trp-Ser-TyrGly-Met-Asn-Pro-Gly-NH2. HPLC and RIA were used for purification followed by Edman degradation for sequence determination. Mass spectroscopy was used to confirm the sequence and amidation of the peptide. The other 2 forms, salmon GnRH and chicken GnRH-II, are identical to the 2 forms found in salmon, which evolved later than whitefish. Synthetic whitefish GnRH is biologically active, as it increased mRNA expression of growth hormone and the a-subunit for LH and thyroid-stimulating hormone in dispersed fish pituitary cells. Our data support the hypothesis that the ancestral salmonid had a third GnRH form when the genome doubled (tetraploidization), but the third form was lost later in some salmonids due to chromosomal rearrangements. We suggest that the salmon GnRH form compensated for the loss of the third form.
