The Experts below are selected from a list of 26358 Experts worldwide ranked by ideXlab platform
Igor Kuzmin - One of the best experts on this subject based on the ideXlab platform.
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construction of a 600 kilobase cosmid clone contig and generation of a transcriptional map surrounding the lung cancer tumor suppressor gene tsg locus on human chromosome 3p21 3 progress toward the isolation of a lung cancer tsg
Cancer Research, 1996Co-Authors: F. Latif, Jeou Yuan Chen, L Geil, Igor Kuzmin, Scott Bader, Yoshitaka Sekido, Vladimir I. Kashuba, Eugene R. Zabarovsky, George Klein, Berton ZbarAbstract:Abstract The critical region on human chromosome 3p21.3 harboring a putative lung cancer tumor suppressor gene (TSG) was previously defined by allelotyping and recently refined by overlapping homozygous deletions. We report the construction of a 700-kb (cosmid and one P1 Phage) clone contig covering the deletion overlap and its flanks. The minimal set of 23 cosmids comprises 600 kb and is extended by one P1 Phage to 700 kb to cover the distal breakpoint of the overlap. The clone contig was extensively characterized by restriction and expression mapping to produce high resolution physical and transcription maps of the cloned region. Potential transcribed fragments were detected by hybridization with PCR-amplified cDNA libraries, direct cDNA selection, “zoo” blotting, cDNA screening, and identification of 24 CpG islands. Thus far, 15 new genes represented by partial or full-length cDNAs were isolated, characterized, and precisely positioned on the contig. Two previously cloned genes, namely GNAI-2 and GNAT-1, were also positioned. In addition, the telomeric breakpoint of the NCI H740 deletion and the centromeric breakpoint of the overlapping GLC20 deletion were discovered and mapped to define precisely the candidate TSG region. This large cosmid clone contig and high resolution maps will prove crucial in the identification of the lung cancer TSG(s).
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construction of a 600 kilobase cosmid clone contig and generation of a transcriptional map surrounding the lung cancer tumor suppressor gene tsg locus on human chromosome 3p21 3 progress toward the isolation of a lung cancer tsg
Cancer Research, 1996Co-Authors: Ming Hui Wei, Jeou Yuan Chen, L Geil, Scott Bader, Yoshitaka Sekido, Vladimir I. Kashuba, F. Latif, Fuh Mei Duh, Cheng Chi Lee, Igor KuzminAbstract:The critical region on human chromosome 3p21.3 harboring a putative lung cancer tumor suppressor gene (TSG) was previously defined by allelotyping and recently refined by overlapping homozygous deletions. We report the construction of a 700-kb (cosmid and one P1 Phage) clone contig covering the deletion overlap and its flanks. The minimal set of 23 cosmids comprises 600 kb and is extended by one P1 Phage to 700 kb to cover the distal breakpoint of the overlap. The clone contig was extensively characterized by restriction and expression mapping to produce high resolution physical and transcription maps of the cloned region. Potential transcribed fragments were detected by hybridization with PCR-amplified cDNA libraries, direct cDNA selection "zoo" blotting, cDNA screening, and identification of 24 CpG islands. Thus far, 15 new genes represented by partial or full-length cDNAs were isolated, characterized, and precisely positioned on the contig. Two previously cloned genes, namely GNAI-2 and GNAT-1, were also positioned. In addition, the telomeric breakpoint of the NCI H740 deletion and centromeric breakpoint of the overlapping GLC20 deletion were discovered and mapped to define precisely the candidate TSG region. This large cosmid clone contig and high resolution maps will prove crucial in the identification of the lung cancer TSG(s).
F. Latif - One of the best experts on this subject based on the ideXlab platform.
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construction of a 600 kilobase cosmid clone contig and generation of a transcriptional map surrounding the lung cancer tumor suppressor gene tsg locus on human chromosome 3p21 3 progress toward the isolation of a lung cancer tsg
Cancer Research, 1996Co-Authors: F. Latif, Jeou Yuan Chen, L Geil, Igor Kuzmin, Scott Bader, Yoshitaka Sekido, Vladimir I. Kashuba, Eugene R. Zabarovsky, George Klein, Berton ZbarAbstract:Abstract The critical region on human chromosome 3p21.3 harboring a putative lung cancer tumor suppressor gene (TSG) was previously defined by allelotyping and recently refined by overlapping homozygous deletions. We report the construction of a 700-kb (cosmid and one P1 Phage) clone contig covering the deletion overlap and its flanks. The minimal set of 23 cosmids comprises 600 kb and is extended by one P1 Phage to 700 kb to cover the distal breakpoint of the overlap. The clone contig was extensively characterized by restriction and expression mapping to produce high resolution physical and transcription maps of the cloned region. Potential transcribed fragments were detected by hybridization with PCR-amplified cDNA libraries, direct cDNA selection, “zoo” blotting, cDNA screening, and identification of 24 CpG islands. Thus far, 15 new genes represented by partial or full-length cDNAs were isolated, characterized, and precisely positioned on the contig. Two previously cloned genes, namely GNAI-2 and GNAT-1, were also positioned. In addition, the telomeric breakpoint of the NCI H740 deletion and the centromeric breakpoint of the overlapping GLC20 deletion were discovered and mapped to define precisely the candidate TSG region. This large cosmid clone contig and high resolution maps will prove crucial in the identification of the lung cancer TSG(s).
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construction of a 600 kilobase cosmid clone contig and generation of a transcriptional map surrounding the lung cancer tumor suppressor gene tsg locus on human chromosome 3p21 3 progress toward the isolation of a lung cancer tsg
Cancer Research, 1996Co-Authors: Ming Hui Wei, Jeou Yuan Chen, L Geil, Scott Bader, Yoshitaka Sekido, Vladimir I. Kashuba, F. Latif, Fuh Mei Duh, Cheng Chi Lee, Igor KuzminAbstract:The critical region on human chromosome 3p21.3 harboring a putative lung cancer tumor suppressor gene (TSG) was previously defined by allelotyping and recently refined by overlapping homozygous deletions. We report the construction of a 700-kb (cosmid and one P1 Phage) clone contig covering the deletion overlap and its flanks. The minimal set of 23 cosmids comprises 600 kb and is extended by one P1 Phage to 700 kb to cover the distal breakpoint of the overlap. The clone contig was extensively characterized by restriction and expression mapping to produce high resolution physical and transcription maps of the cloned region. Potential transcribed fragments were detected by hybridization with PCR-amplified cDNA libraries, direct cDNA selection "zoo" blotting, cDNA screening, and identification of 24 CpG islands. Thus far, 15 new genes represented by partial or full-length cDNAs were isolated, characterized, and precisely positioned on the contig. Two previously cloned genes, namely GNAI-2 and GNAT-1, were also positioned. In addition, the telomeric breakpoint of the NCI H740 deletion and centromeric breakpoint of the overlapping GLC20 deletion were discovered and mapped to define precisely the candidate TSG region. This large cosmid clone contig and high resolution maps will prove crucial in the identification of the lung cancer TSG(s).
Shady Mansour Kamal - One of the best experts on this subject based on the ideXlab platform.
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a recently isolated human commensal escherichia coli st10 clone member mediates enhanced thermotolerance and tetrathionate respiration on a P1 Phage derived incy plasmid
Molecular Microbiology, 2020Co-Authors: Shady Mansour Kamal, Annika Cimdinsahne, Changhan Lee, Alberto J Martinrodriguez, Zaira Seferbekova, Robert Afasizhev, Haleluya Tesfaye Wami, Panagiotis Katikaridis, Lena MeinsAbstract:The ubiquitous human commensal Escherichia coli has been well investigated through its model representative E. coli K-12. In this work, we initially characterized E. coli Fec10, a recently isolated human commensal strain of phylogroup A/sequence type ST10. Compared to E. coli K-12, the 4.88 Mbp Fec10 genome is characterized by distinct single-nucleotide polymorphisms and acquisition of genomic islands. In addition, E. coli Fec10 possesses a 155.86 kbp IncY plasmid, a composite element based on Phage P1. pFec10 harbours multiple cargo genes such as coding for a tetrathionate reductase and its corresponding regulatory two-component system. Among the cargo genes is also the Transmissible Locus of Protein Quality Control (TLPQC), which mediates tolerance to lethal temperatures in bacteria. The disaggregase ClpGGI of TLPQC constitutes a major determinant of the thermotolerance of E. coli Fec10. We confirmed stand-alone disaggregation activity, but observed distinct biochemical characteristics of ClpGGI-Fec10 compared to the nearly identical Pseudomonas aeruginosa ClpGGI-SG17M. Furthermore, we noted a unique contribution of ClpGGI-Fec10 to the exquisite thermotolerance of E. coli Fec10, suggesting functional differences between both disaggregases in vivo. Detection of thermotolerance in 10% of human commensal E. coli isolates hints to the successful establishment of food-borne heat-resistant strains in the human gut.
Berton Zbar - One of the best experts on this subject based on the ideXlab platform.
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construction of a 600 kilobase cosmid clone contig and generation of a transcriptional map surrounding the lung cancer tumor suppressor gene tsg locus on human chromosome 3p21 3 progress toward the isolation of a lung cancer tsg
Cancer Research, 1996Co-Authors: F. Latif, Jeou Yuan Chen, L Geil, Igor Kuzmin, Scott Bader, Yoshitaka Sekido, Vladimir I. Kashuba, Eugene R. Zabarovsky, George Klein, Berton ZbarAbstract:Abstract The critical region on human chromosome 3p21.3 harboring a putative lung cancer tumor suppressor gene (TSG) was previously defined by allelotyping and recently refined by overlapping homozygous deletions. We report the construction of a 700-kb (cosmid and one P1 Phage) clone contig covering the deletion overlap and its flanks. The minimal set of 23 cosmids comprises 600 kb and is extended by one P1 Phage to 700 kb to cover the distal breakpoint of the overlap. The clone contig was extensively characterized by restriction and expression mapping to produce high resolution physical and transcription maps of the cloned region. Potential transcribed fragments were detected by hybridization with PCR-amplified cDNA libraries, direct cDNA selection, “zoo” blotting, cDNA screening, and identification of 24 CpG islands. Thus far, 15 new genes represented by partial or full-length cDNAs were isolated, characterized, and precisely positioned on the contig. Two previously cloned genes, namely GNAI-2 and GNAT-1, were also positioned. In addition, the telomeric breakpoint of the NCI H740 deletion and the centromeric breakpoint of the overlapping GLC20 deletion were discovered and mapped to define precisely the candidate TSG region. This large cosmid clone contig and high resolution maps will prove crucial in the identification of the lung cancer TSG(s).
Vladimir I. Kashuba - One of the best experts on this subject based on the ideXlab platform.
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construction of a 600 kilobase cosmid clone contig and generation of a transcriptional map surrounding the lung cancer tumor suppressor gene tsg locus on human chromosome 3p21 3 progress toward the isolation of a lung cancer tsg
Cancer Research, 1996Co-Authors: F. Latif, Jeou Yuan Chen, L Geil, Igor Kuzmin, Scott Bader, Yoshitaka Sekido, Vladimir I. Kashuba, Eugene R. Zabarovsky, George Klein, Berton ZbarAbstract:Abstract The critical region on human chromosome 3p21.3 harboring a putative lung cancer tumor suppressor gene (TSG) was previously defined by allelotyping and recently refined by overlapping homozygous deletions. We report the construction of a 700-kb (cosmid and one P1 Phage) clone contig covering the deletion overlap and its flanks. The minimal set of 23 cosmids comprises 600 kb and is extended by one P1 Phage to 700 kb to cover the distal breakpoint of the overlap. The clone contig was extensively characterized by restriction and expression mapping to produce high resolution physical and transcription maps of the cloned region. Potential transcribed fragments were detected by hybridization with PCR-amplified cDNA libraries, direct cDNA selection, “zoo” blotting, cDNA screening, and identification of 24 CpG islands. Thus far, 15 new genes represented by partial or full-length cDNAs were isolated, characterized, and precisely positioned on the contig. Two previously cloned genes, namely GNAI-2 and GNAT-1, were also positioned. In addition, the telomeric breakpoint of the NCI H740 deletion and the centromeric breakpoint of the overlapping GLC20 deletion were discovered and mapped to define precisely the candidate TSG region. This large cosmid clone contig and high resolution maps will prove crucial in the identification of the lung cancer TSG(s).
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construction of a 600 kilobase cosmid clone contig and generation of a transcriptional map surrounding the lung cancer tumor suppressor gene tsg locus on human chromosome 3p21 3 progress toward the isolation of a lung cancer tsg
Cancer Research, 1996Co-Authors: Ming Hui Wei, Jeou Yuan Chen, L Geil, Scott Bader, Yoshitaka Sekido, Vladimir I. Kashuba, F. Latif, Fuh Mei Duh, Cheng Chi Lee, Igor KuzminAbstract:The critical region on human chromosome 3p21.3 harboring a putative lung cancer tumor suppressor gene (TSG) was previously defined by allelotyping and recently refined by overlapping homozygous deletions. We report the construction of a 700-kb (cosmid and one P1 Phage) clone contig covering the deletion overlap and its flanks. The minimal set of 23 cosmids comprises 600 kb and is extended by one P1 Phage to 700 kb to cover the distal breakpoint of the overlap. The clone contig was extensively characterized by restriction and expression mapping to produce high resolution physical and transcription maps of the cloned region. Potential transcribed fragments were detected by hybridization with PCR-amplified cDNA libraries, direct cDNA selection "zoo" blotting, cDNA screening, and identification of 24 CpG islands. Thus far, 15 new genes represented by partial or full-length cDNAs were isolated, characterized, and precisely positioned on the contig. Two previously cloned genes, namely GNAI-2 and GNAT-1, were also positioned. In addition, the telomeric breakpoint of the NCI H740 deletion and centromeric breakpoint of the overlapping GLC20 deletion were discovered and mapped to define precisely the candidate TSG region. This large cosmid clone contig and high resolution maps will prove crucial in the identification of the lung cancer TSG(s).
