The Experts below are selected from a list of 12 Experts worldwide ranked by ideXlab platform
Toshiaki Osawa - One of the best experts on this subject based on the ideXlab platform.
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enhancement by il 1β and ifn γ of platelet activation adhesion to leukocytes via gmp 140 PADGEM Protein cd62
Biochemical and Biophysical Research Communications, 1991Co-Authors: Naoko Todoroki, Yoshifumi Watanabe, Toshihiro Akaike, Yasuhiro Katagiri, Kenjiro Tanoue, Hiroh Yamazaki, Tsutomu Tsuji, Satoshi Toyoshima, Toshiaki OsawaAbstract:We have examined the effect of inflammatory cytokines on the platelet activation. IL-1 beta and IFN-gamma were found to enhance the adhesion of thrombin-treated platelets to monocytic leukemia cells (U937), when the adhesion was assayed by platelet-mediated cell agglutination. The agglutination was inhibited by a monoclonal anti-GMP140 antibody or EDTA, suggesting that the enhanced platelet adhesion to the leukemic cells was mediated by GMP140. In addition, these cytokines also increased the release of 5-HT from platelets in the presence of a low concentration of thrombin. These data suggest that platelet functions are regulated by the cytokines and that activated platelets participate in inflammatory process.
J Torresherrera - One of the best experts on this subject based on the ideXlab platform.
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an inhibitory effect of camonagrel a new thromboxane synthase inhibitor on p selectin mediated platelet pmn adhesion
Platelets, 1996Co-Authors: Lukasz Partyka, Aldona Dembinskakiec, Joanna Pankiewicz, Dariusz Dudek, Jadwiga Hartwich, Andrew M Siedlecki, E Pawlus, M Marquezperez, J TorresherreraAbstract:P-selectin (PADGEM Protein, GMP-140 or CD 62) is a glycoProtein of platelet a-granules and endothelial Weibel-Palade bodies that, by mediating cellular adhesion, initiates recruitment of leukocytes and lymphocytes into injured tissue. Both of the endothelial antiplatelet autacoids prostacyclin (PGI2) and nitric oxide (NO) have been demonstrated to inhibit P-selectin expression. Prostaglandin endoperoxides PGG2/PGH2 that are generated by activated platelets have been demonstrated to be used by endothelium for generation of prostacyclin. In an experimental model in vitro that resembles vessel wall/platelet/PMN interaction in vivo, we found that aspirin (100 μM), a COX inhibitor, but not L-NMMA (100 μM) and a NO-synthase inhibitor, reversed the inhibitory effect of arterial wall on P-selectin mediated platelet/PMN adhesion. The anti-adhesive potency of vessel wall reversed by aspirin was dose-dependently restored by camonagrel (3-100 μM), a new TXA2 synthase inhibitor. We conclude that selective TXA2-synthas...
Naoko Todoroki - One of the best experts on this subject based on the ideXlab platform.
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enhancement by il 1β and ifn γ of platelet activation adhesion to leukocytes via gmp 140 PADGEM Protein cd62
Biochemical and Biophysical Research Communications, 1991Co-Authors: Naoko Todoroki, Yoshifumi Watanabe, Toshihiro Akaike, Yasuhiro Katagiri, Kenjiro Tanoue, Hiroh Yamazaki, Tsutomu Tsuji, Satoshi Toyoshima, Toshiaki OsawaAbstract:We have examined the effect of inflammatory cytokines on the platelet activation. IL-1 beta and IFN-gamma were found to enhance the adhesion of thrombin-treated platelets to monocytic leukemia cells (U937), when the adhesion was assayed by platelet-mediated cell agglutination. The agglutination was inhibited by a monoclonal anti-GMP140 antibody or EDTA, suggesting that the enhanced platelet adhesion to the leukemic cells was mediated by GMP140. In addition, these cytokines also increased the release of 5-HT from platelets in the presence of a low concentration of thrombin. These data suggest that platelet functions are regulated by the cytokines and that activated platelets participate in inflammatory process.
Lukasz Partyka - One of the best experts on this subject based on the ideXlab platform.
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an inhibitory effect of camonagrel a new thromboxane synthase inhibitor on p selectin mediated platelet pmn adhesion
Platelets, 1996Co-Authors: Lukasz Partyka, Aldona Dembinskakiec, Joanna Pankiewicz, Dariusz Dudek, Jadwiga Hartwich, Andrew M Siedlecki, E Pawlus, M Marquezperez, J TorresherreraAbstract:P-selectin (PADGEM Protein, GMP-140 or CD 62) is a glycoProtein of platelet a-granules and endothelial Weibel-Palade bodies that, by mediating cellular adhesion, initiates recruitment of leukocytes and lymphocytes into injured tissue. Both of the endothelial antiplatelet autacoids prostacyclin (PGI2) and nitric oxide (NO) have been demonstrated to inhibit P-selectin expression. Prostaglandin endoperoxides PGG2/PGH2 that are generated by activated platelets have been demonstrated to be used by endothelium for generation of prostacyclin. In an experimental model in vitro that resembles vessel wall/platelet/PMN interaction in vivo, we found that aspirin (100 μM), a COX inhibitor, but not L-NMMA (100 μM) and a NO-synthase inhibitor, reversed the inhibitory effect of arterial wall on P-selectin mediated platelet/PMN adhesion. The anti-adhesive potency of vessel wall reversed by aspirin was dose-dependently restored by camonagrel (3-100 μM), a new TXA2 synthase inhibitor. We conclude that selective TXA2-synthas...
Satoshi Toyoshima - One of the best experts on this subject based on the ideXlab platform.
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enhancement by il 1β and ifn γ of platelet activation adhesion to leukocytes via gmp 140 PADGEM Protein cd62
Biochemical and Biophysical Research Communications, 1991Co-Authors: Naoko Todoroki, Yoshifumi Watanabe, Toshihiro Akaike, Yasuhiro Katagiri, Kenjiro Tanoue, Hiroh Yamazaki, Tsutomu Tsuji, Satoshi Toyoshima, Toshiaki OsawaAbstract:We have examined the effect of inflammatory cytokines on the platelet activation. IL-1 beta and IFN-gamma were found to enhance the adhesion of thrombin-treated platelets to monocytic leukemia cells (U937), when the adhesion was assayed by platelet-mediated cell agglutination. The agglutination was inhibited by a monoclonal anti-GMP140 antibody or EDTA, suggesting that the enhanced platelet adhesion to the leukemic cells was mediated by GMP140. In addition, these cytokines also increased the release of 5-HT from platelets in the presence of a low concentration of thrombin. These data suggest that platelet functions are regulated by the cytokines and that activated platelets participate in inflammatory process.
