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Michael Y. Roleda - One of the best experts on this subject based on the ideXlab platform.
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In vitro evaluation of utilisable crude protein and methane production for a diet in which grass silage was replaced by different levels and fractions of extracted seaweed proteins
Animal Feed Science and Technology, 2019Co-Authors: Maryam Ramin, Inga Marie Aasen, Michael Y. Roleda, Marcia De Oliveira Franco, Marten Hetta, Håvard SteinshamnAbstract:Abstract Utilisable crude protein (uCP), methane (CH 4 ) production and other fermentation parameters were analysed in vitro for a diet in which grass silage was replaced by different levels of seaweed protein fractions prepared from three seaweed species: Saccharina latissima , Alaria esculenta and Palmaria palmata . Ten fractions from these three species in which the protein content had been increased and the salt content reduced by simple processing were tested, with inclusion levels in the diet based on the nitrogen content of the fractions. Following an extraction procedure, four fractions from Saccharina latissima , three from Alaria esculenta and one from Palmaria palmata , were gradually included in the diet by replacing high quality silage with approximately 0, 0.15, 0.30 and 0.45 g/g DM, while two high-protein fractions of Palmaria palmata were tested at replacement levels of 0, 0.075, 0.15 and 0.225 g/g DM. To estimate fermentation parameters, 500 mg of each diet were incubated in bottles with 60 mL buffered rumen fluid. Estimated uCP increased linearly with increasing replacement rate of grass silage with seaweed protein fractions (from 158 g/kg DM to 206 g/kg DM on average for all fractions). Increasing protein fraction from the brown seaweed Saccharina latissima in the diet significantly increased true organic matter digestibility (OMD) (from on average 0.786 to 0.821). Organic matter digestibility decreased with increasing level of Alaria esculenta fractions (from on average 0.785 to 0.733), which also gave a linear decrease in CH 4 production (from on average 45.3 to 38.5 mL/g organic matter). As a result of decreased CH 4 production and OMD, total volatile fatty acid concentration decreased with increasing level of Alaria esculenta fractions (from on average 69.5 to 63.0 mmol/L). Thus, positive and species-specific effects of seaweed on estimated uCP and fermentation parameters were observed in vitro when protein fractions remaining after an extraction procedure on seaweed partly replaced grass silage in the feed ration.
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Iodine content in bulk biomass of wild-harvested and cultivated edible seaweeds: Inherent variations determine species-specific daily allowable consumption
Food Chemistry, 2018Co-Authors: Michael Y. Roleda, Céline Rebours, Anna Gietl, Helene Marfaing, Dagmar B Stengel, Jorunn Skjermo, Rosa Jonsdottir, Udo NitschkeAbstract:Abstract This study represents a large-scale investigation into iodine contents in three commercially important and edible seaweed species from the North Atlantic: the brown algae Saccharina latissima and Alaria esculenta , and the red alga Palmaria palmata . Variability among and within species were explored in terms of temporal and spatial variations in addition to biomass source. Mean iodine concentration in bulk seaweed biomass was species-specific: Saccharina > Alaria > Palmaria . Iodine contents of Saccharina biomass were similar between years and seasons, but varied significantly between sampling locations and biomass sources. In Alaria and Palmaria , none of the independent variables examined contributed significantly to the small variations observed. Our data suggest that all three species are rich sources of iodine, and only 32, 283, or 2149 mg dry weight of unprocessed dry biomass of Saccharina , Alaria , or Palmaria , respectively, meets the recommended daily intake levels for most healthy humans.
Udo Nitschke - One of the best experts on this subject based on the ideXlab platform.
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Iodine content in bulk biomass of wild-harvested and cultivated edible seaweeds: Inherent variations determine species-specific daily allowable consumption
Food Chemistry, 2018Co-Authors: Michael Y. Roleda, Céline Rebours, Anna Gietl, Helene Marfaing, Dagmar B Stengel, Jorunn Skjermo, Rosa Jonsdottir, Udo NitschkeAbstract:Abstract This study represents a large-scale investigation into iodine contents in three commercially important and edible seaweed species from the North Atlantic: the brown algae Saccharina latissima and Alaria esculenta , and the red alga Palmaria palmata . Variability among and within species were explored in terms of temporal and spatial variations in addition to biomass source. Mean iodine concentration in bulk seaweed biomass was species-specific: Saccharina > Alaria > Palmaria . Iodine contents of Saccharina biomass were similar between years and seasons, but varied significantly between sampling locations and biomass sources. In Alaria and Palmaria , none of the independent variables examined contributed significantly to the small variations observed. Our data suggest that all three species are rich sources of iodine, and only 32, 283, or 2149 mg dry weight of unprocessed dry biomass of Saccharina , Alaria , or Palmaria , respectively, meets the recommended daily intake levels for most healthy humans.
Chihyen Chien - One of the best experts on this subject based on the ideXlab platform.
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total lower lip reconstruction with a composite radial forearm palmaris longus tendon flap a clinical series
Plastic and Reconstructive Surgery, 2004Co-Authors: Sengfeng Jeng, Yurren Kuo, Fuchan Wei, Chihyen ChienAbstract:Large, full-thickness lip defects after head and neck surgery continue to be a challenge for reconstructive surgeons. The reconstructive aims are to restore the oral lining, the external cheek, oral competence, and function (i.e., articulation, speech, and mastication). The authors' refinement of the composite radial forearm-palmaris longus free flap technique meets these criteria and allows a functional reconstruction of extensive lip and cheek defects in one stage. A composite radial forearm flap including the palmaris longus tendon was designed. The skin flap for the reconstruction of the intraoral lining and the skin defect was folded over the palmaris longus tendon. Both ends of the vascularized tendon were laid through the bilateral modiolus and anchored with adequate tension to the intact orbicularis muscle of the upper lip. This procedure was used in 12 patients. Six patients had cancer of the lower lip, five patients had a buccal cancer involving the lip, and one patient had a primary gum cancer that extended to the lower lip. Total to near-total resection (more than 80 percent) of the lower lip was indicated in six patients. In two other patients, the cancer ablation included more than 80 percent of the lower lip and up to 40 percent of the upper lip. A radial forearm palmaris longus free flap was used in all cases for reconstruction of the defect. Free flap survival was 100 percent. At the time of final evaluation, which was 1 year after the operation, all patients had good oral continence at rest (static suspension) and had achieved sufficient oral competence when eating. Ten patients were able to resume a regular diet, and two patients could eat a soft diet. All patients regained normal or near-normal speech and had an acceptable appearance. The described refinement of the composite radial palmaris longus free flap technique allows the reconstruction of the lower lip with a functioning oral sphincter; the technique can be recommended for patients who need large lower lip resection. It provides functional recovery of the reconstructed lower lip synchronizing with the remaining upper lip.
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total lower lip reconstruction with a composite radial forearm palmaris longus tendon flap a clinical series
Plastic and Reconstructive Surgery, 2004Co-Authors: Sengfeng Jeng, Chihying Su, Chihyen ChienAbstract:Large, full-thickness lip defects after head and neck surgery continue to be a challenge for reconstructive surgeons. The reconstructive aims are to restore the oral lining, the external cheek, oral competence, and function (i.e., articulation, speech, and mastication). The authors' refinement of the composite radial forearm-palmaris longus free flap technique meets these criteria and allows a functional reconstruction of extensive lip and cheek defects in one stage. A composite radial forearm flap including the palmaris longus tendon was designed. The skin flap for the reconstruction of the intraoral lining and the skin defect was folded over the palmaris longus tendon. Both ends of the vascularized tendon were laid through the bilateral modiolus and anchored with adequate tension to the intact orbicularis muscle of the upper lip. This procedure was used in 12 patients. Six patients had cancer of the lower lip, five patients had a buccal cancer involving the lip, and one patient had a primary gum cancer that extended to the lower lip. Total to neartotal resection (more than 80 percent) of the lower lip was indicated in six patients. In two other patients, the cancer ablation included more than 80 percent of the lower lip and up to 40 percent of the upper lip. A radial forearm palmaris longus free flap was used in all cases for reconstruction of the defect. Free flap survival was 100 percent. At the time of final evaluation, which was 1 year after the operation, all patients had good oral continence at rest (static suspension) and had achieved sufficient oral competence when eating. Ten patients were able to resume a regular diet, and two patients could eat a soft diet. All patients regained normal or near-normal speech and had an acceptable appearance. The described refinement of the composite radial palmaris longus free flap technique allows the reconstruction of the lower lip with a functioning oral sphincter; the technique can be recommended for patients who need large lower lip resection. It provides functional recovery of the reconstructed lower lip synchronizing with the remaining upper lip. (Plast. Reconstr.
Sengfeng Jeng - One of the best experts on this subject based on the ideXlab platform.
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total lower lip reconstruction with a composite radial forearm palmaris longus tendon flap a clinical series
Plastic and Reconstructive Surgery, 2004Co-Authors: Sengfeng Jeng, Yurren Kuo, Fuchan Wei, Chihyen ChienAbstract:Large, full-thickness lip defects after head and neck surgery continue to be a challenge for reconstructive surgeons. The reconstructive aims are to restore the oral lining, the external cheek, oral competence, and function (i.e., articulation, speech, and mastication). The authors' refinement of the composite radial forearm-palmaris longus free flap technique meets these criteria and allows a functional reconstruction of extensive lip and cheek defects in one stage. A composite radial forearm flap including the palmaris longus tendon was designed. The skin flap for the reconstruction of the intraoral lining and the skin defect was folded over the palmaris longus tendon. Both ends of the vascularized tendon were laid through the bilateral modiolus and anchored with adequate tension to the intact orbicularis muscle of the upper lip. This procedure was used in 12 patients. Six patients had cancer of the lower lip, five patients had a buccal cancer involving the lip, and one patient had a primary gum cancer that extended to the lower lip. Total to near-total resection (more than 80 percent) of the lower lip was indicated in six patients. In two other patients, the cancer ablation included more than 80 percent of the lower lip and up to 40 percent of the upper lip. A radial forearm palmaris longus free flap was used in all cases for reconstruction of the defect. Free flap survival was 100 percent. At the time of final evaluation, which was 1 year after the operation, all patients had good oral continence at rest (static suspension) and had achieved sufficient oral competence when eating. Ten patients were able to resume a regular diet, and two patients could eat a soft diet. All patients regained normal or near-normal speech and had an acceptable appearance. The described refinement of the composite radial palmaris longus free flap technique allows the reconstruction of the lower lip with a functioning oral sphincter; the technique can be recommended for patients who need large lower lip resection. It provides functional recovery of the reconstructed lower lip synchronizing with the remaining upper lip.
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total lower lip reconstruction with a composite radial forearm palmaris longus tendon flap a clinical series
Plastic and Reconstructive Surgery, 2004Co-Authors: Sengfeng Jeng, Chihying Su, Chihyen ChienAbstract:Large, full-thickness lip defects after head and neck surgery continue to be a challenge for reconstructive surgeons. The reconstructive aims are to restore the oral lining, the external cheek, oral competence, and function (i.e., articulation, speech, and mastication). The authors' refinement of the composite radial forearm-palmaris longus free flap technique meets these criteria and allows a functional reconstruction of extensive lip and cheek defects in one stage. A composite radial forearm flap including the palmaris longus tendon was designed. The skin flap for the reconstruction of the intraoral lining and the skin defect was folded over the palmaris longus tendon. Both ends of the vascularized tendon were laid through the bilateral modiolus and anchored with adequate tension to the intact orbicularis muscle of the upper lip. This procedure was used in 12 patients. Six patients had cancer of the lower lip, five patients had a buccal cancer involving the lip, and one patient had a primary gum cancer that extended to the lower lip. Total to neartotal resection (more than 80 percent) of the lower lip was indicated in six patients. In two other patients, the cancer ablation included more than 80 percent of the lower lip and up to 40 percent of the upper lip. A radial forearm palmaris longus free flap was used in all cases for reconstruction of the defect. Free flap survival was 100 percent. At the time of final evaluation, which was 1 year after the operation, all patients had good oral continence at rest (static suspension) and had achieved sufficient oral competence when eating. Ten patients were able to resume a regular diet, and two patients could eat a soft diet. All patients regained normal or near-normal speech and had an acceptable appearance. The described refinement of the composite radial palmaris longus free flap technique allows the reconstruction of the lower lip with a functioning oral sphincter; the technique can be recommended for patients who need large lower lip resection. It provides functional recovery of the reconstructed lower lip synchronizing with the remaining upper lip. (Plast. Reconstr.
Annevalerie Gallandirmouli - One of the best experts on this subject based on the ideXlab platform.
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one step purification of r phycoerythrin from the red macroalga Palmaria palmata using preparative polyacrylamide gel electrophoresis
Journal of Chromatography B: Biomedical Sciences and Applications, 2000Co-Authors: Michel Lucon, Annevalerie Gallandirmouli, L Pons, N T Mrabet, Joel FleurenceAbstract:Abstract Phycoerythrin is a major light-harvesting pigment of red algae and cyanobacteria widely used as a fluorescent probe. In this study, phycoerythrin of the red macroalga Palmaria palmata was extracted by grinding the algal sample in liquid nitrogen, homogenisation in phosphate buffer and centrifugation. Phycoerythrin was then purified from this crude extract using preparative polyacrylamide gel electrophoresis (PAGE) with a continuous elution system and detected by its pink colour and fluorescence. The pigment presented a typical spectrum of R-phycoerythrin, with three absorbance maxima at 499, 545 and 565 nm, and displayed a fluorescence maximum at 578 nm. The absorbance ratio A 565 / A 280 , a criterion for purity, was 3.2. A single protein of relative molecular mass 240 000 was detected on native-PAGE with silver staining. Sodium dodecyl sulphate–PAGE demonstrated the presence of two major subunits with M r 20 000 and 21 000, respectively, and a very minor subunit of M r 30 000. These observations are consistent with the (αβ) 6 γ subunit composition characteristic of R-phycoerythrin. Phycoerythrin of Palmaria palmata was determined to be present in larger amounts in autumn and showed a good stability up to 60°C and between pH 3.5 and 9.5. In conclusion, phycoerythrin of Palmaria palmata was purified in a single-step using preparative PAGE. Obtaining pure R-phycoerythrin of Palmaria palmata will allow one to evaluate its fluorescence properties for future applications in biochemical techniques.
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nutritional value of proteins from edible seaweed Palmaria palmata dulse
Journal of Nutritional Biochemistry, 1999Co-Authors: Joel Fleurence, Annevalerie Gallandirmouli, Radia Lamghari, Michel Lucon, Catherine Rouxel, Olivier Barbaroux, Jean-pierre Bronowicki, Christian VillaumeAbstract:Abstract Palmaria palmata (Dulse) is a red seaweed that may be a potential protein source in the human diet. Its protein content, amino acid composition, and protein digestibility were studied with algae collected every month over a 1-year period. Significant variations in protein content were observed according to the season: The highest protein content (21.9 ± 3.5%) was found in the winter–spring period and the lowest (11.9 ± 2.0%) in the summer–early autumn period. Most of the essential amino acids were present throughout the year. After 6-hour in vitro digestion in a cell dialysis using porcine pepsin and porcine pancreatin, the digestibility of proteins from Palmaria palmata crude powder, represented by dialyzed nitrogen, was estimated at 29.52 ± 1.47%. Relative digestibility was 56%, using casein hydrolysis as 100% reference digestibility. In vitro digestibility of proteins extracted in water was analyzed by sodium dodecylsulfate polyacrylamide gel electrophoresis using either bovine trypsin, bovine chymotrypsin, pronase from Streptomyces griseus, or human intestinal juice. Dulse proteins were hydrolyzed to a limited extent, which confirmed a rather low digestibility. Hydrolysis rate was higher with trypsin and lower with chymotrypsin compared with the two other enzymatic systems, pronase and intestinal juice, respectively. The association of algal powder and protein extract to casein and bovine serum albumin, respectively, produced a significant decrease in the hydrolysis rate of the standard proteins. In conclusion, the digestibility of Palmaria palmata proteins seems to be limited by the algae non-proteic fraction.
