Panax Ginseng

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Kazuhiro Hirakura - One of the best experts on this subject based on the ideXlab platform.

Hiroshi Mitsuhashi - One of the best experts on this subject based on the ideXlab platform.

Yu Yang - One of the best experts on this subject based on the ideXlab platform.

  • Functional characterization of a WRKY family gene involved in somatic embryogenesis in Panax Ginseng
    Protoplasma, 2019
    Co-Authors: Yu Yang, Ni Wang, Shoujing Zhao
    Abstract:

    As a perennial herbaceous species, Panax Ginseng is widely cultivated and used as traditional herbal medicine. The root of Panax Ginseng commonly remains expensive as conventional breeding of Panax Ginseng is difficult. Somatic embryogenesis (S.E.) is a useful tool for plant propagation and optimal model for understanding the mechanisms of plant embryogenesis. In Panax Ginseng, increasing studies have been widely performed to optimize the technology of S.E., while the underlying mechanism remains unclear. In this paper, we cloned and identified a WRKY family gene named PgWRKY6 which is upregulated in response to 2,4-D (2,4-dichlorophenoxyacetic acid)-induced embryogenic callus development. The silencing of PgWRKY6 obviously reduces the induction rate of embryogenic callus, indicating its crucial role in S.E. of Panax Ginseng hairy root. The expressions of several ROS-scavenging genes are also inducible during embryogenic callus development, and the transcriptions of PgGST, PgAPX1, and PgSOD are demonstrated to be regulated by PgWRKY6. Recombinant PgWRKY6, an approximate 40-KDa protein purified from Escherichia coli, shows a specific DNA-binding activity with a potential recognition site of TTGAC(C/T). This work demonstrated that as a conserved WRKY family transcription factor, PgWRKY6 functions upstream of PgGST, PgAPX1, and PgSOD, and potentially mediated auxins -ROS signaling pathway in the process of S.E. in Panax species.

  • Purification and Characterization of Glucosyltransferase from Panax Ginseng Hairy Root Cultures
    Pharmaceutical biotechnology, 2009
    Co-Authors: Yu Yang
    Abstract:

    To study the biotransformaion zymologic basis of the Panax Ginseng hairy root cultures, the crude enzyme of glucosyltransferase from Panax Ginseng hairy root cultures was made by the fractional precipitation of ammonium sulphate. After the procedure of Hiprep Q ion-exchange chromatography, a 566 k glucosyltransferase was isolated. The apparent Km value for p-hydroxybenzoic acid was 0399nmol/L, and its Vmaxvalue was 274 nmol/min. Its activity was optimal in pH80 and at 45℃, and the enzyme was stable from pH40 to pH100 under the condition of temperature below 60℃. In the ion study, the enzyme activity was slightly enhanced by Fe2+ and Cu2+, but inhibited by Na+, K+, Mg2+, Ca2+, Zn2+, Ag+and Ba2+. Mn2+ and Co2+ had very slight effect on the activity of the glucosyltransferase. All the results were coincident with the provious glucosyltransferase research and this was the first report on the glucosyltransferase from the Panax Ginseng hairy root cultures.

Yukinobu Ikeya - One of the best experts on this subject based on the ideXlab platform.

Makoto Morita - One of the best experts on this subject based on the ideXlab platform.