The Experts below are selected from a list of 930 Experts worldwide ranked by ideXlab platform
Grace C.h. Yang - One of the best experts on this subject based on the ideXlab platform.
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ultrafast Papanicolaou Stain and cell transfer technique enhance cytologic diagnosis of hodgkin lymphoma
Diagnostic Cytopathology, 2002Co-Authors: Maryann D Gangi, Grace C.h. YangAbstract:Diagnosis of Hodgkin lymphoma (HL) by fine-needle aspiration (FNA) is often hampered by aspirated blood that camouflage scattered Hodgkin cells and Reed-Sternberg (HRS) cells, and the absence of HRS cells in the smears submitted for immunophenotyping. The objective of this study was to develop a simple protocol to overcome these problems. The visibility of HRS cells in Diff-Quik, traditional, and Ultrafast Papanicolaou (UFP) Stains in FNA smears were compared in 73 cases of HL. HRS cells were found to be most visible in UFP because of the hemolysis of aspirated blood and the highlighting of HRS cells by the red-Staining nucleoli. UFP-Stained smears containing HRS cells were subsequently immunophenotyped via the cell-transfer technique. UFP Staining was found to have no deleterious effect on the immunoreactivity of cellular CD15 and CD30 antigens of HRS cells. This simple protocol enhances the cytologic diagnosis of HL, feasible even with a single smear.
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ultrasound guided fine needle aspiration of the thyroid assessed by ultrafast Papanicolaou Stain data from 1135 biopsies with a two to six year follow up
Thyroid, 2001Co-Authors: Grace C.h. Yang, Doreen Liebeskind, Albert V MessinaAbstract:One of the limitations of fine-needle aspiration (FNA) of the thyroid is difficulty in distinguishing the follicular variant (FV) of papillary thyroid carcinomas (PTC) from follicular neoplasms. By highlighting the "Orphan Annie-eyed" clear nuclei of the former, the Ultrafast Papanicolaou Stain (UFP) easily separates these two entities. One thousand one hundred thirty-five ultrasound-guided FNAs of the thyroid were assessed by UFP with immediate biopsy results reported to the patients in a busy radiology office in Manhattan from November 1994 to December 1998. Of the 77 thyroid cancers resected, 22 were FVPTC and 17 were microcarcinomas (1 medullary carcinoma, 16 PTC). The rates of "unsatisfactory," "cancer," "suspicious for cancer," "follicular neoplasm," and "benign" cytology were 0.7%, 4.4%, 2.6%, 10.2%, and 82.1%, respectively and the cancer yields at surgery were 98%, 81.8%, 15.8%, and 0% respectively. Of the 1127 satisfactory FNAs in the series with a 2- to -6 years of clinical follow-up, a false-ne...
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application of ultrafast Papanicolaou Stain to body fluid cytology
Acta Cytologica, 2001Co-Authors: Grace C.h. Yang, Juliana Papellas, Jerry WaismanAbstract:OBJECTIVE: To investigate the applicability of the UItrafast Papanicolaou Stain to the cytology of fluids and to compare it with other methods. STUDY DESIGN: Over a 30-month period, 528 unfixed fluids (462 serous effusions, 48 pelvic washings, 16 cyst fluids and 2 bile duct drain fluids) were mixed thoroughly and centrifuged. Two Swedish-style air-dried smears were made and Stained with Diff-Quik (Mercedes Medical, Inc., Sarasota, Florida, U.S.A.) and Ultrafast Papanicolaou Stain (Richard Allan Scientific, Kalamazoo, Michigan, U.S.A.), and the remaining sediment was fixed in CytoRich Red (TriPath Imaging, Inc., Burlington, North Carolina, U.S.A.), centrifuged onto a 17.5-mm circle with a Hettich cytocentrifuge and Stained by the Papanicolaou method. RESULTS: For the 115 malignant fluids, Ultrafast Papanicolaou Stain was the preferred method in the 94 nonhematopoietic malignant fluids, Diff-Quik was the preferred method in the 9 hematopoietic malignancies, and CytoRich Red was the preferred preparation in 8 bloody effusions containing rare cancer cells and 4 malignant pelvic washings. The diagnostic turnaround time of smears Stained by Ultrafast Papanicolaou Stain was <15 minutes, fast enough for intraoperative consultations. CONCLUSION: It seems that Ultrafast Papanicolaou Stain improves the resolution of cytoplasmic and nuclear details of nonhematopoietic cells in body fluids. However, to detect cancer in all types of fluids, Diff-Quik and CytoRich preparations are also required. We now examine three slides per fluid sample, one slide by each of the three techniques.
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clear nuclei of papillary thyroid carcinoma conspicuous in fine needle aspiration and intraoperative smears processed by ultrafast Papanicolaou Stain
Modern Pathology, 1997Co-Authors: Grace C.h. Yang, E GreenebaumAbstract:The Orphan Annie-eyed clear nucleus, defined as a large, optically clear nucleus, devoid of chromatin strands, with sharp chromatin rim, is a more specific feature than are nuclear grooves or intranuclear cytoplasmic inclusions in papillary thyroid carcinoma. In addition, this characteristic nuclear feature is detectable at low magnification. Although these clear nuclei are routinely seen in paraffin sections, they are inconspicuously seen in conventionally processed touch-imprints and fine-needle aspiration (FNA) smears. Among our two institutions, there have been 148 thyroid cases processed by Ultrafast Papanicolaou Stain (UFP), including 43 papillary carcinomas, 38 cellular follicular lesions, and 67 cases of nodular hyperplasia. We observed clear nuclei in all of the cases of UFP-processed FNA and intraoperative smears of papillary carcinoma but not of other thyroid lesions. The clear nuclei are most evident in tumor cells with direct contact to the glass slide and are not seen in tumor cells soaked in cystic fluid. UFP is a valuable way to detect Orphan Annie-eyed clear nuclei of papillary thyroid carcinoma early in the diagnostic evaluation, either at immediate on-site evaluation of FNA or at intraoperative consultation and before the availability of permanent sections.
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Combined Use of the “Scratch and Smear” Sampling Technique and Ultrafast Papanicolaou Stain for Intraoperative Cytology
Acta cytologica, 1997Co-Authors: Grace C.h. Yang, Syed A. HodaAbstract:OBJECTIVE: To assess the applicability of Ultrafast Papanicolaou Stain (UFP), a 90-second, high-resolution Stain incorporating the air-dried rehydration technique, to intraoperative cytology in surgical pathology laboratories. STUDY DESIGN: Two hundred sixty-two randomly selected surgical specimens for intraoperative consultations were included in the study. Each specimen was first conventionally prepared by touch imprinting, wet fixation in alcohol and Staining by hematoxylin and eosin (HE the remaining specimens were then sampled by the scratch and smear technique and subjected to the UFP Staining protocol. RESULTS: The combined use of the scratch and smear sampling technique and UFP Staining was superior to the conventional methods of touch imprinting and H&E Staining in every case because of the following features: (1) intact tissue fragments composed of both epithelial and stromal elements rather than epithelial cells only, (2) polychromasia rather than bichromasia, (3) sharper nuclear and cytoplasmic details, (4) histologic criteria applicable to thick tissue fragments due to transparency and flatness and (5) clear background devoid of red blood cells and eosin. CONCLUSION: The combined use of the scratch and smear sampling technique and UFP enhances intraoperative cytology.
Yuanyuan Song - One of the best experts on this subject based on the ideXlab platform.
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thinprep plus Papanicolaou Stain method is more sensitive than cytospin coupled wright giems Stain method in cerebrospinal fluid cytology for diagnosis of leptomeningeal metastasis from solid tumors
PLOS ONE, 2015Co-Authors: Guozi Yang, Yongxiang Wang, Hua He, Xiaochuan Pang, Yu Li, Lihua Dong, Yuanyuan SongAbstract:Background The present study was designed to determine whether the Thinprep plus Papanicolaou Stain (Thinprep) method is more sensitive than the Cytospin-coupled Wright-Giemsa (WG) Stain (Cytospin) method in diagnosis of leptomeningeal metastasis (LM) from malignant solid tumors in cerebrospinal fluid (CSF). We also explored if the Thinprep method could be used in the differential diagnosis of the type of primary tumor cells based on the morphology of tumor cells in CSF samples. Methods The morphological features of tumor cells in fresh CSF samples were analyzed using both methods. The tumor cell detection rates were compared between the two methods. Results Using the Thinprep method, we found that each type of tumor cells in the CSF samples had specific identifiable morphological features linked to their primary cancer origins, such as adenocarcinomas originated from the lungs, breast, and stomach, and lung squamous cell carcinomas, small cell lung cancer, large-cell neuroendocrine lung cancer, hepatocellular carcinoma, and malignant melanoma. In a retrospective study with 88 LM patients, cancer cells were detected in 80 out of the 88 CSF samples. In the comparative study with 45 LM patients, the initial detection rate of the Thinprep method was significantly higher than that of the Cytospin method (73.3% vs. 57.8%, P<0.01). The cell morphology was better preserved and subcellular structures were clearer using the Thinprep method, compared to the Cytospin method. Conclusions The Thinprep method is more sensitive and suitable for LM diagnosis in CSF in patients with malignant solid tumors than the Cytospin method. The Thinprep method may facilitate primary tumor detection and help design early treatment regimens for LM patients with tumors of unknown primary origin.
Tseching Chen - One of the best experts on this subject based on the ideXlab platform.
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use of riu Stain in the immediate interpretation of bronchial brushing cytology
Acta Cytologica, 1997Co-Authors: Chenghuei Lee, Chienying Liu, Chunhua Wang, Tseching ChenAbstract:OBJECTIVE: To determine the usefulness of Riu Stain in the immediate interpretation of cytologic material from bronchoscopic brushings and its accuracy in the diagnosis of lung malignancies. STUDY DESIGN: A prospectively comparative study between Riu and Papanicolaou Stain with pathologic control was conducted. Eighty-six patients with central lung lesions, visible during fiberoptic bronchoscopy, were studied. Endobronchial biopsies and brushings were performed at the time of the same procedure. RESULTS: The diagnostic accuracy of Riu Stain for mal lignancy was 97%, with a sensitivity of 99%, specificity of 85%, positive predictive value of 97% and negative predictive value of 92%. As for the Papanicolaou technique, the diagnostic accuracy for malignancy was 97%, with a sensitivity of 97%, negative of 92%, positive predictive value of 99% and negative predictive value of 86%. The overall cytologic typing accuracy of Riu Stain was 86%, which was comparable with that of Papanicolaou Stain (90%, P=.48). Both techniques are more accurate in the diagnosis ofsquamous carcinoma, small cell carcinoma and adenocarcinoma than in large cell carcinoma. CONCLUSION: Riu Stain is rapid, simple to use and, most important, accurate for the identification of lung malignancies. It can increase the diagnostic success rate, prevent unnecessary repeat bronchoscopies and lower related complications.
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use of riu Stain in the immediate interpretation of bronchial brushing cytology comparison with Papanicolaou Stain and histology
Acta Cytologica, 1997Co-Authors: Chenghuei Lee, Chienying Liu, Chunhua Wang, Tseching ChenAbstract:OBJECTIVE: To determine the usefulness of Riu Stain in the immediate interpretation of cytologic material from bronchoscopic brushings and its accuracy in the diagnosis of lung malignancies. STUDY DESIGN: A prospectively comparative study between Riu and Papanicolaou Stain with pathologic control was conducted. Eighty-six patients with central lung lesions, visible during fiberoptic bronchoscopy, were studied. Endobronchial biopsies and brushings were performed at the time of the same procedure. RESULTS: The diagnostic accuracy of Riu Stain for mal lignancy was 97%, with a sensitivity of 99%, specificity of 85%, positive predictive value of 97% and negative predictive value of 92%. As for the Papanicolaou technique, the diagnostic accuracy for malignancy was 97%, with a sensitivity of 97%, negative of 92%, positive predictive value of 99% and negative predictive value of 86%. The overall cytologic typing accuracy of Riu Stain was 86%, which was comparable with that of Papanicolaou Stain (90%, P=.48). Both techniques are more accurate in the diagnosis ofsquamous carcinoma, small cell carcinoma and adenocarcinoma than in large cell carcinoma. CONCLUSION: Riu Stain is rapid, simple to use and, most important, accurate for the identification of lung malignancies. It can increase the diagnostic success rate, prevent unnecessary repeat bronchoscopies and lower related complications.
Guozi Yang - One of the best experts on this subject based on the ideXlab platform.
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thinprep plus Papanicolaou Stain method is more sensitive than cytospin coupled wright giems Stain method in cerebrospinal fluid cytology for diagnosis of leptomeningeal metastasis from solid tumors
PLOS ONE, 2015Co-Authors: Guozi Yang, Yongxiang Wang, Hua He, Xiaochuan Pang, Yu Li, Lihua Dong, Yuanyuan SongAbstract:Background The present study was designed to determine whether the Thinprep plus Papanicolaou Stain (Thinprep) method is more sensitive than the Cytospin-coupled Wright-Giemsa (WG) Stain (Cytospin) method in diagnosis of leptomeningeal metastasis (LM) from malignant solid tumors in cerebrospinal fluid (CSF). We also explored if the Thinprep method could be used in the differential diagnosis of the type of primary tumor cells based on the morphology of tumor cells in CSF samples. Methods The morphological features of tumor cells in fresh CSF samples were analyzed using both methods. The tumor cell detection rates were compared between the two methods. Results Using the Thinprep method, we found that each type of tumor cells in the CSF samples had specific identifiable morphological features linked to their primary cancer origins, such as adenocarcinomas originated from the lungs, breast, and stomach, and lung squamous cell carcinomas, small cell lung cancer, large-cell neuroendocrine lung cancer, hepatocellular carcinoma, and malignant melanoma. In a retrospective study with 88 LM patients, cancer cells were detected in 80 out of the 88 CSF samples. In the comparative study with 45 LM patients, the initial detection rate of the Thinprep method was significantly higher than that of the Cytospin method (73.3% vs. 57.8%, P<0.01). The cell morphology was better preserved and subcellular structures were clearer using the Thinprep method, compared to the Cytospin method. Conclusions The Thinprep method is more sensitive and suitable for LM diagnosis in CSF in patients with malignant solid tumors than the Cytospin method. The Thinprep method may facilitate primary tumor detection and help design early treatment regimens for LM patients with tumors of unknown primary origin.
E S Stroll - One of the best experts on this subject based on the ideXlab platform.
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diff quik Stain as a simplified alternative to Papanicolaou Stain for determination of quality of endocervical specimens submitted for pcr detection of chlamydia trachomatis
Journal of Clinical Microbiology, 1996Co-Authors: James A Kellogg, J W Seiple, J L Klinedinst, E S StrollAbstract:The simple, rapid, two-step Diff-Quik Stain procedure (Baxter Diagnostics) was compared with the Papanicolaou Stain for microscopic determination of endocervical specimen quality. Results from 230 (98.7%) of 233 specimens Stained by both methods indicated agreement between the two Staining methods for detection of the endocervical cells or erythrocytes indicating specimen adequacy. By using the Amplicor Chlamydia trachomatis Test (Roche Diagnostic Systems) to detect C.trachomatis and the Diff-Quik Stain to assess specimen adequacy, PCR-positive results were obtained from 147 (9.1%) of 1,615 microscopically adequate specimens but from only 13 (2.2%) of the 583 inadequate specimens (P < 0.001).
