The Experts below are selected from a list of 1276422 Experts worldwide ranked by ideXlab platform
Todd Ponsky - One of the best experts on this subject based on the ideXlab platform.
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White Paper: Technology for surgical telementoring—SAGES Project 6 Technology Working Group
Surgical Endoscopy, 2019Co-Authors: Etai M. Bogen, Christopher M. Schlachta, Todd PonskyAbstract:Background Recent advances in telecommunication Technology and video conferencing systems have opened a new avenue for surgical instruction called “surgical telementoring.” This report from the Technology Working Group of the SAGES Project 6 Summit reviews the telementoring Technology that currently exists and proposes recommendations for minimum Technology requirements and future Technology development. While also providing insight in regulatory considerations, this review offers what prospective surgical telementoring participants need to know about the underlying Technology with a specific focus on safety, reliability, transmission quality, ease of use, and cost. Methods Content experts from around the world, in minimally invasive surgery, surgical mentoring and telementoring, surgical education, business development, healthcare innovation, and regulation were invited to attend a 2-day summit in Los Angeles, USA to outline the current state of surgical telementoring and chart the challenges and opportunities going forward. This article summarizes the discussion, conclusions, and recommendation of the Technology group with regard to telementoring Technology. Results This article reviews the technical requirements which can be divided into the following categories: (1) safety, (2) reliability, (3) transmission quality, (4) ease of use, and (5) cost. Conclusion Telementoring applications are Technology driven. Given the pace of change of Technology, guiding principles in Technology design and selection are warranted (Table 4). Telementoring technologies require two basic components, video capturing and display devices at the transmitting and receiving end, and a telecommunication link between them. Many additional features can be added to this basic setup including multiple cameras or video sources, remote camera zoom and pan, recording and storage of videos and images, and telestration capabilities to mention just a few. In general, the cost of these technologies is feature driven. The education framework for each specific application should determine the need for these features (Schlachta in Surg Endosc https://doi.org/10.1007/s00464-016-4988-5 ).
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white Paper Technology for surgical telementoring sages project 6 Technology working group
Surgical Endoscopy and Other Interventional Techniques, 2019Co-Authors: Etai M. Bogen, Christopher M. Schlachta, Todd PonskyAbstract:Background Recent advances in telecommunication Technology and video conferencing systems have opened a new avenue for surgical instruction called “surgical telementoring.” This report from the Technology Working Group of the SAGES Project 6 Summit reviews the telementoring Technology that currently exists and proposes recommendations for minimum Technology requirements and future Technology development. While also providing insight in regulatory considerations, this review offers what prospective surgical telementoring participants need to know about the underlying Technology with a specific focus on safety, reliability, transmission quality, ease of use, and cost.
Elizabeth M Johnson - One of the best experts on this subject based on the ideXlab platform.
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ultra rapid preparation of total genomic dna from isolates of yeast and mould using whatman fta filter Paper Technology a reusable dna archiving system
Medical Mycology, 2006Co-Authors: Andrew M Borman, Christopher J Linton, Sarahjane Miles, Colin K Campbell, Elizabeth M JohnsonAbstract:Conventional methods for purifying PCR-grade fungal genomic DNA typically require cell disruption (either physical or enzymatic) coupled with laborious organic extraction and precipitation stages, or expensive column-based technologies. Here we present an easy and extremely rapid method of preparing yeast and mould genomic DNAs from living cultures using Whatman FTA filter matrix Technology. Aqueous suspensions of yeast cells or hyphal fragments and conidia (in the case of moulds) are applied directly (or after freeze-thawing) to dry FTA filters. Inoculated filters are then subjected to brief microwave treatment, to dry the filters and inactivate the organisms. Filter punches are removed, washed rapidly, dried and placed directly into PCR reactions. We show that this procedure inactivated all of the 38 yeast and 75 mould species tested, and generated PCR-grade DNA preparations in around 15 minutes. A total of 218 out of 226 fungal isolates tested liberated amplifiable DNA after application to FTA filters....
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ultra rapid preparation of total genomic dna from isolates of yeast and mould using whatman fta filter Paper Technology a reusable dna archiving system
Medical Mycology, 2006Co-Authors: Andrew M Borman, Christopher J Linton, Sarahjane Miles, Colin K Campbell, Elizabeth M JohnsonAbstract:Conventional methods for purifying PCR-grade fungal genomic DNA typically require cell disruption (either physical or enzymatic) coupled with laborious organic extraction and precipitation stages, or expensive column-based technologies. Here we present an easy and extremely rapid method of preparing yeast and mould genomic DNAs from living cultures using Whatman FTA filter matrix Technology. Aqueous suspensions of yeast cells or hyphal fragments and conidia (in the case of moulds) are applied directly (or after freeze-thawing) to dry FTA filters. Inoculated filters are then subjected to brief microwave treatment, to dry the filters and inactivate the organisms. Filter punches are removed, washed rapidly, dried and placed directly into PCR reactions. We show that this procedure inactivated all of the 38 yeast and 75 mould species tested, and generated PCR-grade DNA preparations in around 15 minutes. A total of 218 out of 226 fungal isolates tested liberated amplifiable DNA after application to FTA filters. Detection limits with yeast cultures were approximately 10 colony-forming units per punch. Moreover, we demonstrate that filter punches can be recovered after PCR, washed and used in fresh PCR reactions without detectable cross-contamination. Whatman FTA Technology thus represents a cheap, ultra-rapid method of fungal genomic DNA preparation, and also potentially represents a powerful fungal DNA archiving and storage system.
Etai M. Bogen - One of the best experts on this subject based on the ideXlab platform.
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White Paper: Technology for surgical telementoring—SAGES Project 6 Technology Working Group
Surgical Endoscopy, 2019Co-Authors: Etai M. Bogen, Christopher M. Schlachta, Todd PonskyAbstract:Background Recent advances in telecommunication Technology and video conferencing systems have opened a new avenue for surgical instruction called “surgical telementoring.” This report from the Technology Working Group of the SAGES Project 6 Summit reviews the telementoring Technology that currently exists and proposes recommendations for minimum Technology requirements and future Technology development. While also providing insight in regulatory considerations, this review offers what prospective surgical telementoring participants need to know about the underlying Technology with a specific focus on safety, reliability, transmission quality, ease of use, and cost. Methods Content experts from around the world, in minimally invasive surgery, surgical mentoring and telementoring, surgical education, business development, healthcare innovation, and regulation were invited to attend a 2-day summit in Los Angeles, USA to outline the current state of surgical telementoring and chart the challenges and opportunities going forward. This article summarizes the discussion, conclusions, and recommendation of the Technology group with regard to telementoring Technology. Results This article reviews the technical requirements which can be divided into the following categories: (1) safety, (2) reliability, (3) transmission quality, (4) ease of use, and (5) cost. Conclusion Telementoring applications are Technology driven. Given the pace of change of Technology, guiding principles in Technology design and selection are warranted (Table 4). Telementoring technologies require two basic components, video capturing and display devices at the transmitting and receiving end, and a telecommunication link between them. Many additional features can be added to this basic setup including multiple cameras or video sources, remote camera zoom and pan, recording and storage of videos and images, and telestration capabilities to mention just a few. In general, the cost of these technologies is feature driven. The education framework for each specific application should determine the need for these features (Schlachta in Surg Endosc https://doi.org/10.1007/s00464-016-4988-5 ).
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white Paper Technology for surgical telementoring sages project 6 Technology working group
Surgical Endoscopy and Other Interventional Techniques, 2019Co-Authors: Etai M. Bogen, Christopher M. Schlachta, Todd PonskyAbstract:Background Recent advances in telecommunication Technology and video conferencing systems have opened a new avenue for surgical instruction called “surgical telementoring.” This report from the Technology Working Group of the SAGES Project 6 Summit reviews the telementoring Technology that currently exists and proposes recommendations for minimum Technology requirements and future Technology development. While also providing insight in regulatory considerations, this review offers what prospective surgical telementoring participants need to know about the underlying Technology with a specific focus on safety, reliability, transmission quality, ease of use, and cost.
Christopher M. Schlachta - One of the best experts on this subject based on the ideXlab platform.
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White Paper: Technology for surgical telementoring—SAGES Project 6 Technology Working Group
Surgical Endoscopy, 2019Co-Authors: Etai M. Bogen, Christopher M. Schlachta, Todd PonskyAbstract:Background Recent advances in telecommunication Technology and video conferencing systems have opened a new avenue for surgical instruction called “surgical telementoring.” This report from the Technology Working Group of the SAGES Project 6 Summit reviews the telementoring Technology that currently exists and proposes recommendations for minimum Technology requirements and future Technology development. While also providing insight in regulatory considerations, this review offers what prospective surgical telementoring participants need to know about the underlying Technology with a specific focus on safety, reliability, transmission quality, ease of use, and cost. Methods Content experts from around the world, in minimally invasive surgery, surgical mentoring and telementoring, surgical education, business development, healthcare innovation, and regulation were invited to attend a 2-day summit in Los Angeles, USA to outline the current state of surgical telementoring and chart the challenges and opportunities going forward. This article summarizes the discussion, conclusions, and recommendation of the Technology group with regard to telementoring Technology. Results This article reviews the technical requirements which can be divided into the following categories: (1) safety, (2) reliability, (3) transmission quality, (4) ease of use, and (5) cost. Conclusion Telementoring applications are Technology driven. Given the pace of change of Technology, guiding principles in Technology design and selection are warranted (Table 4). Telementoring technologies require two basic components, video capturing and display devices at the transmitting and receiving end, and a telecommunication link between them. Many additional features can be added to this basic setup including multiple cameras or video sources, remote camera zoom and pan, recording and storage of videos and images, and telestration capabilities to mention just a few. In general, the cost of these technologies is feature driven. The education framework for each specific application should determine the need for these features (Schlachta in Surg Endosc https://doi.org/10.1007/s00464-016-4988-5 ).
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white Paper Technology for surgical telementoring sages project 6 Technology working group
Surgical Endoscopy and Other Interventional Techniques, 2019Co-Authors: Etai M. Bogen, Christopher M. Schlachta, Todd PonskyAbstract:Background Recent advances in telecommunication Technology and video conferencing systems have opened a new avenue for surgical instruction called “surgical telementoring.” This report from the Technology Working Group of the SAGES Project 6 Summit reviews the telementoring Technology that currently exists and proposes recommendations for minimum Technology requirements and future Technology development. While also providing insight in regulatory considerations, this review offers what prospective surgical telementoring participants need to know about the underlying Technology with a specific focus on safety, reliability, transmission quality, ease of use, and cost.
Andrew M Borman - One of the best experts on this subject based on the ideXlab platform.
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ultra rapid preparation of total genomic dna from isolates of yeast and mould using whatman fta filter Paper Technology a reusable dna archiving system
Medical Mycology, 2006Co-Authors: Andrew M Borman, Christopher J Linton, Sarahjane Miles, Colin K Campbell, Elizabeth M JohnsonAbstract:Conventional methods for purifying PCR-grade fungal genomic DNA typically require cell disruption (either physical or enzymatic) coupled with laborious organic extraction and precipitation stages, or expensive column-based technologies. Here we present an easy and extremely rapid method of preparing yeast and mould genomic DNAs from living cultures using Whatman FTA filter matrix Technology. Aqueous suspensions of yeast cells or hyphal fragments and conidia (in the case of moulds) are applied directly (or after freeze-thawing) to dry FTA filters. Inoculated filters are then subjected to brief microwave treatment, to dry the filters and inactivate the organisms. Filter punches are removed, washed rapidly, dried and placed directly into PCR reactions. We show that this procedure inactivated all of the 38 yeast and 75 mould species tested, and generated PCR-grade DNA preparations in around 15 minutes. A total of 218 out of 226 fungal isolates tested liberated amplifiable DNA after application to FTA filters....
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ultra rapid preparation of total genomic dna from isolates of yeast and mould using whatman fta filter Paper Technology a reusable dna archiving system
Medical Mycology, 2006Co-Authors: Andrew M Borman, Christopher J Linton, Sarahjane Miles, Colin K Campbell, Elizabeth M JohnsonAbstract:Conventional methods for purifying PCR-grade fungal genomic DNA typically require cell disruption (either physical or enzymatic) coupled with laborious organic extraction and precipitation stages, or expensive column-based technologies. Here we present an easy and extremely rapid method of preparing yeast and mould genomic DNAs from living cultures using Whatman FTA filter matrix Technology. Aqueous suspensions of yeast cells or hyphal fragments and conidia (in the case of moulds) are applied directly (or after freeze-thawing) to dry FTA filters. Inoculated filters are then subjected to brief microwave treatment, to dry the filters and inactivate the organisms. Filter punches are removed, washed rapidly, dried and placed directly into PCR reactions. We show that this procedure inactivated all of the 38 yeast and 75 mould species tested, and generated PCR-grade DNA preparations in around 15 minutes. A total of 218 out of 226 fungal isolates tested liberated amplifiable DNA after application to FTA filters. Detection limits with yeast cultures were approximately 10 colony-forming units per punch. Moreover, we demonstrate that filter punches can be recovered after PCR, washed and used in fresh PCR reactions without detectable cross-contamination. Whatman FTA Technology thus represents a cheap, ultra-rapid method of fungal genomic DNA preparation, and also potentially represents a powerful fungal DNA archiving and storage system.
