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Elizabeth García - One of the best experts on this subject based on the ideXlab platform.
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Prevalence of skin diseases in children 1 to 6 years old in the city of Bogota, Colombia.
The World Allergy Organization journal, 2020Co-Authors: Elizabeth García, Evelyne Halpert, Elizabeth Borrero, Pablo Chaparro, Jorge Molina, Milciades Ibáñez, Maritza TorresAbstract:Abstract Background Skin diseases represent an important part of the morbidity among children and are possibly influenced by geographic, racial, social, cultural, and economic factors. Despite being so frequent around the world, skin diseases have not been important in developing strategies in public health. Aim The purpose of this study was to evaluate the prevalence of skin diseases among the student population between 1 and 6 years of age in Bogota, Colombia between March 2009 and June 2011. Materials and methods This cross-sectional study was performed across a probabilistic, stratified, randomized sampling by proportional assignment (based on locality and type of institution) and was developed in schools in Bogota, Colombia. Results A total of 2437 children between 1 and 6 years of age were examined, and 42.8% (1035) presented a dermatologic disease. Papular Urticaria was the most frequent (62.9%) (IC: 58.4%; 67.1%), followed by dermatitis/eczema (13.0%) (IC: 10.8%; 15.4%), and infectious diseases (12.3%) (IC: 9.7%; 15.3%). Conclusion The research demonstrates a high prevalence of Papular Urticaria as a result of flea and mosquito bites and infectious diseases of the skin in the studied population. The dermatologic diseases found are easy to diagnose, respond to the proper treatment, and are preventable. However, the fact that many of the examined children likely had not visited the doctor for the detected pathology could indicate the lack of access to health services affecting this population.
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Prevalence of Papular Urticaria caused by flea bites and associated factors in children 1-6 years of age in Bogotá, D.C.
The World Allergy Organization journal, 2017Co-Authors: Evelyne Halpert, Elizabeth Borrero, Milcíades Ibáñez-pinilla, Pablo Chaparro, Jorge Molina, Maritza Torres, Elizabeth GarcíaAbstract:Papular Urticaria is a chronic inflammatory disease caused by exposure to arthropod bites. The disease has been reported in children attending medical centers, but the causes as the risk factors associated with the disease have not been established. The objective of this study was to determine the prevalence of Papular Urticaria caused by flea bite and identify the risk factors in children between 1 to 6 years of age in Bogota D.C, between March 2009 and June 2011. A cross-sectional, two-stage, clustered study using random probability sampling and stratified with proportional allocation was carried out in children (1–6 years of age) in educational institutions in Bogota D.C. to determine the prevalence of the disease. Children underwent a dermatological examination by general practitioners with a previous training. Furthermore, digital photographs of skin lesions were taken for further confirmation of the diagnosis by dermatologists. A structured survey was completed by the parents or caregivers, and it was evaluated using an unconditional logistic regression to identify factors associated with the disease. A total of 2437 children were included in the study. The prevalence of Papular Urticaria caused by flea bite in this population was 20.3% (CI 95%: 18.2 to 22.5%). The major risk factors associated with the disease were the presence of fleas in households (OR 1.74, CI 95%: 1.35 to 2.25), using mattresses without springs (OR 1.73, CI 95%: 1.20 to 2.50), the use of daily public transportation to carry the children to the educational institutions (OR 1.76, CI 95%: 1.07 to 2.89), having a soil/earth floor in the main bedroom (OR 6.81, CI 95%:1.16–39.96), and having siblings with a history of atopic dermatitis (OR 1.76 CI 95%: 1.07–2.89). A high prevalence of Papular Urticaria caused by flea bite was found in Bogota D.C. The main factors associated with the disease might be modified with the implementation of prevention, control strategies in housing, educational institutions, and public transportation.
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Prevalence of Papular Urticaria caused by flea bites and associated factors in children 1–6 years of age in Bogotá, D.C.
World Allergy Organization Journal, 2017Co-Authors: Evelyne Halpert, Elizabeth Borrero, Milcíades Ibáñez-pinilla, Pablo Chaparro, Jorge Molina, Maritza Torres, Elizabeth GarcíaAbstract:Background Papular Urticaria is a chronic inflammatory disease caused by exposure to arthropod bites. The disease has been reported in children attending medical centers, but the causes as the risk factors associated with the disease have not been established. The objective of this study was to determine the prevalence of Papular Urticaria caused by flea bite and identify the risk factors in children between 1 to 6 years of age in Bogotá D.C, between March 2009 and June 2011. Methods A cross-sectional, two-stage, clustered study using random probability sampling and stratified with proportional allocation was carried out in children (1–6 years of age) in educational institutions in Bogotá D.C. to determine the prevalence of the disease. Children underwent a dermatological examination by general practitioners with a previous training. Furthermore, digital photographs of skin lesions were taken for further confirmation of the diagnosis by dermatologists. A structured survey was completed by the parents or caregivers, and it was evaluated using an unconditional logistic regression to identify factors associated with the disease. Results A total of 2437 children were included in the study. The prevalence of Papular Urticaria caused by flea bite in this population was 20.3% (CI 95%: 18.2 to 22.5%). The major risk factors associated with the disease were the presence of fleas in households (OR 1.74, CI 95%: 1.35 to 2.25), using mattresses without springs (OR 1.73, CI 95%: 1.20 to 2.50), the use of daily public transportation to carry the children to the educational institutions (OR 1.76, CI 95%: 1.07 to 2.89), having a soil/earth floor in the main bedroom (OR 6.81, CI 95%:1.16–39.96), and having siblings with a history of atopic dermatitis (OR 1.76 CI 95%: 1.07–2.89). Conclusions A high prevalence of Papular Urticaria caused by flea bite was found in Bogotá D.C. The main factors associated with the disease might be modified with the implementation of prevention, control strategies in housing, educational institutions, and public transportation.
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Urticaria Papular y sus agentes causales en Colombia
Biomedica : revista del Instituto Nacional de Salud, 2016Co-Authors: Ana Lozano, Josefina Zakzuk, Juan Felipe López, Elizabeth GarcíaAbstract:Papular Urticaria is a chronic allergic reaction induced by insect bites, which is common in the tropics. The objective of this review was to deepen on epidemiological and immunological aspects of this disease, focused on data published in Latin American countries.We conducted a non-systematic review of the literature through electronic search on the epidemiology of Papular Urticaria, the entomological characteristics of the causative agents and associated immunological mechanisms.Several reports from medical centers suggest that Papular Urticaria is common in Latin America. Only one epidemiological survey designed to estimate prevalence of Papular Urticaria has been published, reporting that about a quarter of children under six years of age is affected by this condition in Bogota. There is evidence on the causal relationship among exposure to indoor fleas, poverty and Papular Urticaria in Bogota, a representative city of the Andean altitudes. Information about causal insects in tropical warmer areas is scarce, although from clinical reports Aedes aegypti and Culex quienquefasciatus appear to be the most common. Th2 cellular-mediated mechanisms are involved in its pathogenesis, which explains its delayed hypersensitivity. The role of immunoglobulin E is not clear in this disease. Insect-derived antigens directly involved in Papular Urticaria etiology are unknown. However, it is possible that common molecules among causal insects mediate cross-reactive reactions, such as Cte f 2 allergen, found in cat fleas, and its counterparts in mosquitoes.Papular Urticaria is a frequent disease in Latin America that should be further investigated. Immunological characterization of the molecular components that cause this condition may solve questions about its pathogenesis.
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Differences in IgE mediated basophil degranulation induced by proteic fractions from whole flea body extract in patients with Papular Urticaria by flea bite and healthy controls
World Allergy Organization Journal, 2013Co-Authors: Omar Dominguez-amorocho, Adriana Cuéllar, Evelyne Halpert, Elizabeth García, Silvia Duarte, Adriana RodríguezAbstract:Background Papular Urticaria by flea bite (PUFB) is a chronic inflammatory disease in children. The aim of this study was to assess the functional activity of IgE to protein fractions from flea body extract, through basophil degranulation in PUFB patients and controls. Methods Basophil degranulation, measured by overexpression of CD63 surface molecules, was evaluated by flow cytometry in samples from patients and controls. Cell stimulation was performed with three fractions with different molecular weight from flea body extract using a Basotest® modified protocol. Mann–Whitney U -test was used for comparisons. Results Specific IgE from PUFB patients and healthy controls induced basophil degranulation to flea body extract with no significant differences between them (16.2 ± 3.1% vs 13.6 ± 2.8% p = 0.77). However, when flea extract was analyzed in fractions with proteins ranging different molecular weights, significant differences were observed on the response from patients compared with controls to
Evelyne Halpert - One of the best experts on this subject based on the ideXlab platform.
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Prevalence of skin diseases in children 1 to 6 years old in the city of Bogota, Colombia.
The World Allergy Organization journal, 2020Co-Authors: Elizabeth García, Evelyne Halpert, Elizabeth Borrero, Pablo Chaparro, Jorge Molina, Milciades Ibáñez, Maritza TorresAbstract:Abstract Background Skin diseases represent an important part of the morbidity among children and are possibly influenced by geographic, racial, social, cultural, and economic factors. Despite being so frequent around the world, skin diseases have not been important in developing strategies in public health. Aim The purpose of this study was to evaluate the prevalence of skin diseases among the student population between 1 and 6 years of age in Bogota, Colombia between March 2009 and June 2011. Materials and methods This cross-sectional study was performed across a probabilistic, stratified, randomized sampling by proportional assignment (based on locality and type of institution) and was developed in schools in Bogota, Colombia. Results A total of 2437 children between 1 and 6 years of age were examined, and 42.8% (1035) presented a dermatologic disease. Papular Urticaria was the most frequent (62.9%) (IC: 58.4%; 67.1%), followed by dermatitis/eczema (13.0%) (IC: 10.8%; 15.4%), and infectious diseases (12.3%) (IC: 9.7%; 15.3%). Conclusion The research demonstrates a high prevalence of Papular Urticaria as a result of flea and mosquito bites and infectious diseases of the skin in the studied population. The dermatologic diseases found are easy to diagnose, respond to the proper treatment, and are preventable. However, the fact that many of the examined children likely had not visited the doctor for the detected pathology could indicate the lack of access to health services affecting this population.
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Prevalence of Papular Urticaria caused by flea bites and associated factors in children 1-6 years of age in Bogotá, D.C.
The World Allergy Organization journal, 2017Co-Authors: Evelyne Halpert, Elizabeth Borrero, Milcíades Ibáñez-pinilla, Pablo Chaparro, Jorge Molina, Maritza Torres, Elizabeth GarcíaAbstract:Papular Urticaria is a chronic inflammatory disease caused by exposure to arthropod bites. The disease has been reported in children attending medical centers, but the causes as the risk factors associated with the disease have not been established. The objective of this study was to determine the prevalence of Papular Urticaria caused by flea bite and identify the risk factors in children between 1 to 6 years of age in Bogota D.C, between March 2009 and June 2011. A cross-sectional, two-stage, clustered study using random probability sampling and stratified with proportional allocation was carried out in children (1–6 years of age) in educational institutions in Bogota D.C. to determine the prevalence of the disease. Children underwent a dermatological examination by general practitioners with a previous training. Furthermore, digital photographs of skin lesions were taken for further confirmation of the diagnosis by dermatologists. A structured survey was completed by the parents or caregivers, and it was evaluated using an unconditional logistic regression to identify factors associated with the disease. A total of 2437 children were included in the study. The prevalence of Papular Urticaria caused by flea bite in this population was 20.3% (CI 95%: 18.2 to 22.5%). The major risk factors associated with the disease were the presence of fleas in households (OR 1.74, CI 95%: 1.35 to 2.25), using mattresses without springs (OR 1.73, CI 95%: 1.20 to 2.50), the use of daily public transportation to carry the children to the educational institutions (OR 1.76, CI 95%: 1.07 to 2.89), having a soil/earth floor in the main bedroom (OR 6.81, CI 95%:1.16–39.96), and having siblings with a history of atopic dermatitis (OR 1.76 CI 95%: 1.07–2.89). A high prevalence of Papular Urticaria caused by flea bite was found in Bogota D.C. The main factors associated with the disease might be modified with the implementation of prevention, control strategies in housing, educational institutions, and public transportation.
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Prevalence of Papular Urticaria caused by flea bites and associated factors in children 1–6 years of age in Bogotá, D.C.
World Allergy Organization Journal, 2017Co-Authors: Evelyne Halpert, Elizabeth Borrero, Milcíades Ibáñez-pinilla, Pablo Chaparro, Jorge Molina, Maritza Torres, Elizabeth GarcíaAbstract:Background Papular Urticaria is a chronic inflammatory disease caused by exposure to arthropod bites. The disease has been reported in children attending medical centers, but the causes as the risk factors associated with the disease have not been established. The objective of this study was to determine the prevalence of Papular Urticaria caused by flea bite and identify the risk factors in children between 1 to 6 years of age in Bogotá D.C, between March 2009 and June 2011. Methods A cross-sectional, two-stage, clustered study using random probability sampling and stratified with proportional allocation was carried out in children (1–6 years of age) in educational institutions in Bogotá D.C. to determine the prevalence of the disease. Children underwent a dermatological examination by general practitioners with a previous training. Furthermore, digital photographs of skin lesions were taken for further confirmation of the diagnosis by dermatologists. A structured survey was completed by the parents or caregivers, and it was evaluated using an unconditional logistic regression to identify factors associated with the disease. Results A total of 2437 children were included in the study. The prevalence of Papular Urticaria caused by flea bite in this population was 20.3% (CI 95%: 18.2 to 22.5%). The major risk factors associated with the disease were the presence of fleas in households (OR 1.74, CI 95%: 1.35 to 2.25), using mattresses without springs (OR 1.73, CI 95%: 1.20 to 2.50), the use of daily public transportation to carry the children to the educational institutions (OR 1.76, CI 95%: 1.07 to 2.89), having a soil/earth floor in the main bedroom (OR 6.81, CI 95%:1.16–39.96), and having siblings with a history of atopic dermatitis (OR 1.76 CI 95%: 1.07–2.89). Conclusions A high prevalence of Papular Urticaria caused by flea bite was found in Bogotá D.C. The main factors associated with the disease might be modified with the implementation of prevention, control strategies in housing, educational institutions, and public transportation.
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Differences in IgE mediated basophil degranulation induced by proteic fractions from whole flea body extract in patients with Papular Urticaria by flea bite and healthy controls
World Allergy Organization Journal, 2013Co-Authors: Omar Dominguez-amorocho, Adriana Cuéllar, Evelyne Halpert, Elizabeth García, Silvia Duarte, Adriana RodríguezAbstract:Background Papular Urticaria by flea bite (PUFB) is a chronic inflammatory disease in children. The aim of this study was to assess the functional activity of IgE to protein fractions from flea body extract, through basophil degranulation in PUFB patients and controls. Methods Basophil degranulation, measured by overexpression of CD63 surface molecules, was evaluated by flow cytometry in samples from patients and controls. Cell stimulation was performed with three fractions with different molecular weight from flea body extract using a Basotest® modified protocol. Mann–Whitney U -test was used for comparisons. Results Specific IgE from PUFB patients and healthy controls induced basophil degranulation to flea body extract with no significant differences between them (16.2 ± 3.1% vs 13.6 ± 2.8% p = 0.77). However, when flea extract was analyzed in fractions with proteins ranging different molecular weights, significant differences were observed on the response from patients compared with controls to
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Differences in IgE mediated basophil degranulation induced by proteic fractions from whole flea body extract in patients with Papular Urticaria by flea bite and healthy controls.
The World Allergy Organization journal, 2013Co-Authors: Omar Dominguez-amorocho, Adriana Cuéllar, Evelyne Halpert, Elizabeth García, Silvia Duarte, Adriana RodríguezAbstract:Background Papular Urticaria by flea bite (PUFB) is a chronic inflammatory disease in children. The aim of this study was to assess the functional activity of IgE to protein fractions from flea body extract, through basophil degranulation in PUFB patients and controls.
Adriana Cuéllar - One of the best experts on this subject based on the ideXlab platform.
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Differences in IgE mediated basophil degranulation induced by proteic fractions from whole flea body extract in patients with Papular Urticaria by flea bite and healthy controls
World Allergy Organization Journal, 2013Co-Authors: Omar Dominguez-amorocho, Adriana Cuéllar, Evelyne Halpert, Elizabeth García, Silvia Duarte, Adriana RodríguezAbstract:Background Papular Urticaria by flea bite (PUFB) is a chronic inflammatory disease in children. The aim of this study was to assess the functional activity of IgE to protein fractions from flea body extract, through basophil degranulation in PUFB patients and controls. Methods Basophil degranulation, measured by overexpression of CD63 surface molecules, was evaluated by flow cytometry in samples from patients and controls. Cell stimulation was performed with three fractions with different molecular weight from flea body extract using a Basotest® modified protocol. Mann–Whitney U -test was used for comparisons. Results Specific IgE from PUFB patients and healthy controls induced basophil degranulation to flea body extract with no significant differences between them (16.2 ± 3.1% vs 13.6 ± 2.8% p = 0.77). However, when flea extract was analyzed in fractions with proteins ranging different molecular weights, significant differences were observed on the response from patients compared with controls to
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Differences in IgE mediated basophil degranulation induced by proteic fractions from whole flea body extract in patients with Papular Urticaria by flea bite and healthy controls.
The World Allergy Organization journal, 2013Co-Authors: Omar Dominguez-amorocho, Adriana Cuéllar, Evelyne Halpert, Elizabeth García, Silvia Duarte, Adriana RodríguezAbstract:Background Papular Urticaria by flea bite (PUFB) is a chronic inflammatory disease in children. The aim of this study was to assess the functional activity of IgE to protein fractions from flea body extract, through basophil degranulation in PUFB patients and controls.
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Differences in systemic and skin migrating-specific CD4 T cells in Papular Urticaria by flea bite.
International archives of allergy and immunology, 2012Co-Authors: Omar Dominguez-amorocho, Evelyne Halpert, Elizabeth García, Silvia Duarte, Adriana Rodríguez, John Mario González, María Claudia Ortega, Adriana CuéllarAbstract:Background: Papular Urticaria by flea bite is a chronic allergic condition in which clinical improvement may occur at the age of 7 years, thus representing a natu
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Specific pattern of flea antigen recognition by IgG subclass and IgE during the progression of Papular Urticaria caused by flea bite.
Allergologia Et Immunopathologia, 2010Co-Authors: Adriana Cuéllar, Evelyne Halpert, Alberto Gomez, Adriana Rojas, F. Rojas, A Rodriguez, Elizabeth GarcíaAbstract:BACKGROUND: Papular Urticaria caused by flea bite presents clinical symptoms of a hypersensitivity reaction accompanied by skin lesions. However, the pattern of recognition by different antibody isotypes during the progression of the disease is unknown. This study evaluated variations in immunoglobulin E and immunoglobulin G subclass antibody responses to flea antigens during the progression of Papular Urticaria caused by flea bite METHODS: Twenty-five patients clinically diagnosed with Papular Urticaria due to flea bite were included. Ten healthy children were included as controls. Recognition of antigens from complete flea body extract by patients and healthy controls was determined using immunoblot assays. RESULTS: The results revealed that patients with 2-5 years of Papular Urticaria evidenced more IgE bands than those with shorter or longer durations of symptoms. In contrast, healthy children showed a predominance of immunoglobulin G1 and immunoglobulin G3. The majority of the recognised antigens were low molecular weight proteins (
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Specific pattern of flea antigen recognition by IgG subclass and IgE during the progression of Papular Urticaria caused by flea bite
Allergologia et immunopathologia, 2010Co-Authors: Adriana Cuéllar, Evelyne Halpert, Alberto Gomez, Adriana Rojas, F. Rojas, Adriana Rodríguez, Elizabeth GarcíaAbstract:Abstract Background Papular Urticaria caused by flea bite presents clinical symptoms of a hypersensitivity reaction accompanied by skin lesions. However, the pattern of recognition by different antibody isotypes during the progression of the disease is unknown. This study evaluated variations in immunoglobulin E and immunoglobulin G subclass antibody responses to flea antigens during the progression of Papular Urticaria caused by flea bite Methods Twenty-five patients clinically diagnosed with Papular Urticaria due to flea bite were included. Ten healthy children were included as controls. Recognition of antigens from complete flea body extract by patients and healthy controls was determined using immunoblot assays. Results The results revealed that patients with 2–5 years of Papular Urticaria evidenced more IgE bands than those with shorter or longer durations of symptoms. In contrast, healthy children showed a predominance of immunoglobulin G1 and immunoglobulin G3. The majority of the recognised antigens were low molecular weight proteins ( Conclusion The predominant specific antibody isotypes vary according to the time elapsed since the onset of symptoms in Papular Urticaria caused by flea bite.
Adriana Rodríguez - One of the best experts on this subject based on the ideXlab platform.
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Differences in IgE mediated basophil degranulation induced by proteic fractions from whole flea body extract in patients with Papular Urticaria by flea bite and healthy controls
World Allergy Organization Journal, 2013Co-Authors: Omar Dominguez-amorocho, Adriana Cuéllar, Evelyne Halpert, Elizabeth García, Silvia Duarte, Adriana RodríguezAbstract:Background Papular Urticaria by flea bite (PUFB) is a chronic inflammatory disease in children. The aim of this study was to assess the functional activity of IgE to protein fractions from flea body extract, through basophil degranulation in PUFB patients and controls. Methods Basophil degranulation, measured by overexpression of CD63 surface molecules, was evaluated by flow cytometry in samples from patients and controls. Cell stimulation was performed with three fractions with different molecular weight from flea body extract using a Basotest® modified protocol. Mann–Whitney U -test was used for comparisons. Results Specific IgE from PUFB patients and healthy controls induced basophil degranulation to flea body extract with no significant differences between them (16.2 ± 3.1% vs 13.6 ± 2.8% p = 0.77). However, when flea extract was analyzed in fractions with proteins ranging different molecular weights, significant differences were observed on the response from patients compared with controls to
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Differences in IgE mediated basophil degranulation induced by proteic fractions from whole flea body extract in patients with Papular Urticaria by flea bite and healthy controls.
The World Allergy Organization journal, 2013Co-Authors: Omar Dominguez-amorocho, Adriana Cuéllar, Evelyne Halpert, Elizabeth García, Silvia Duarte, Adriana RodríguezAbstract:Background Papular Urticaria by flea bite (PUFB) is a chronic inflammatory disease in children. The aim of this study was to assess the functional activity of IgE to protein fractions from flea body extract, through basophil degranulation in PUFB patients and controls.
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Differences in systemic and skin migrating-specific CD4 T cells in Papular Urticaria by flea bite.
International archives of allergy and immunology, 2012Co-Authors: Omar Dominguez-amorocho, Evelyne Halpert, Elizabeth García, Silvia Duarte, Adriana Rodríguez, John Mario González, María Claudia Ortega, Adriana CuéllarAbstract:Background: Papular Urticaria by flea bite is a chronic allergic condition in which clinical improvement may occur at the age of 7 years, thus representing a natu
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Specific pattern of flea antigen recognition by IgG subclass and IgE during the progression of Papular Urticaria caused by flea bite
Allergologia et immunopathologia, 2010Co-Authors: Adriana Cuéllar, Evelyne Halpert, Alberto Gomez, Adriana Rojas, F. Rojas, Adriana Rodríguez, Elizabeth GarcíaAbstract:Abstract Background Papular Urticaria caused by flea bite presents clinical symptoms of a hypersensitivity reaction accompanied by skin lesions. However, the pattern of recognition by different antibody isotypes during the progression of the disease is unknown. This study evaluated variations in immunoglobulin E and immunoglobulin G subclass antibody responses to flea antigens during the progression of Papular Urticaria caused by flea bite Methods Twenty-five patients clinically diagnosed with Papular Urticaria due to flea bite were included. Ten healthy children were included as controls. Recognition of antigens from complete flea body extract by patients and healthy controls was determined using immunoblot assays. Results The results revealed that patients with 2–5 years of Papular Urticaria evidenced more IgE bands than those with shorter or longer durations of symptoms. In contrast, healthy children showed a predominance of immunoglobulin G1 and immunoglobulin G3. The majority of the recognised antigens were low molecular weight proteins ( Conclusion The predominant specific antibody isotypes vary according to the time elapsed since the onset of symptoms in Papular Urticaria caused by flea bite.
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differential th1 th2 balance in peripheral blood lymphocytes from patients suffering from flea bite induced Papular Urticaria
Allergologia Et Immunopathologia, 2009Co-Authors: Adriana Cuéllar, Evelyne Halpert, Alberto Gomez, F. Rojas, Adriana Rodríguez, Elizabeth GarcíaAbstract:Abstract Background The Th1/Th2 balance has not been characterized in patients suffering from flea bite-induced Papular Urticaria (FBPU). Our aim was to improve understanding of the immunopathogenesis of CD4+ and CD8+ T-cells in humans suffering from flea bite-induced Papular Urticaria. Methods Peripheral blood mononuclear cells were obtained from 18 pediatric patients and 10 age-matched healthy controls. Cellular phenotypes, intracellular production of interferon gamma (IFNγ) and interleukin-4 (IL-4) in T-cells stimulated with polyclonal stimuli was determined by flow cytometry following short-term in vitro stimulation. Results The results revealed lower frequencies of IFNγ-secreting (p = 0.02) and higher frequencies of IL-4-secreting (p = 0.03) CD4+ T-cells in patient lymphocyte cultures compared to healthy control cultures in the presence of polyclonal stimuli. This is the first description of differential cytokine patterns in Papular Urticaria patients. Conclusion Patients suffering from Papular Urticaria have an atopic status compared to healthy children.
Alberto Gomez - One of the best experts on this subject based on the ideXlab platform.
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Specific pattern of flea antigen recognition by IgG subclass and IgE during the progression of Papular Urticaria caused by flea bite.
Allergologia Et Immunopathologia, 2010Co-Authors: Adriana Cuéllar, Evelyne Halpert, Alberto Gomez, Adriana Rojas, F. Rojas, A Rodriguez, Elizabeth GarcíaAbstract:BACKGROUND: Papular Urticaria caused by flea bite presents clinical symptoms of a hypersensitivity reaction accompanied by skin lesions. However, the pattern of recognition by different antibody isotypes during the progression of the disease is unknown. This study evaluated variations in immunoglobulin E and immunoglobulin G subclass antibody responses to flea antigens during the progression of Papular Urticaria caused by flea bite METHODS: Twenty-five patients clinically diagnosed with Papular Urticaria due to flea bite were included. Ten healthy children were included as controls. Recognition of antigens from complete flea body extract by patients and healthy controls was determined using immunoblot assays. RESULTS: The results revealed that patients with 2-5 years of Papular Urticaria evidenced more IgE bands than those with shorter or longer durations of symptoms. In contrast, healthy children showed a predominance of immunoglobulin G1 and immunoglobulin G3. The majority of the recognised antigens were low molecular weight proteins (
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Specific pattern of flea antigen recognition by IgG subclass and IgE during the progression of Papular Urticaria caused by flea bite
Allergologia et immunopathologia, 2010Co-Authors: Adriana Cuéllar, Evelyne Halpert, Alberto Gomez, Adriana Rojas, F. Rojas, Adriana Rodríguez, Elizabeth GarcíaAbstract:Abstract Background Papular Urticaria caused by flea bite presents clinical symptoms of a hypersensitivity reaction accompanied by skin lesions. However, the pattern of recognition by different antibody isotypes during the progression of the disease is unknown. This study evaluated variations in immunoglobulin E and immunoglobulin G subclass antibody responses to flea antigens during the progression of Papular Urticaria caused by flea bite Methods Twenty-five patients clinically diagnosed with Papular Urticaria due to flea bite were included. Ten healthy children were included as controls. Recognition of antigens from complete flea body extract by patients and healthy controls was determined using immunoblot assays. Results The results revealed that patients with 2–5 years of Papular Urticaria evidenced more IgE bands than those with shorter or longer durations of symptoms. In contrast, healthy children showed a predominance of immunoglobulin G1 and immunoglobulin G3. The majority of the recognised antigens were low molecular weight proteins ( Conclusion The predominant specific antibody isotypes vary according to the time elapsed since the onset of symptoms in Papular Urticaria caused by flea bite.
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differential th1 th2 balance in peripheral blood lymphocytes from patients suffering from flea bite induced Papular Urticaria
Allergologia Et Immunopathologia, 2009Co-Authors: Adriana Cuéllar, Evelyne Halpert, Alberto Gomez, F. Rojas, Adriana Rodríguez, Elizabeth GarcíaAbstract:Abstract Background The Th1/Th2 balance has not been characterized in patients suffering from flea bite-induced Papular Urticaria (FBPU). Our aim was to improve understanding of the immunopathogenesis of CD4+ and CD8+ T-cells in humans suffering from flea bite-induced Papular Urticaria. Methods Peripheral blood mononuclear cells were obtained from 18 pediatric patients and 10 age-matched healthy controls. Cellular phenotypes, intracellular production of interferon gamma (IFNγ) and interleukin-4 (IL-4) in T-cells stimulated with polyclonal stimuli was determined by flow cytometry following short-term in vitro stimulation. Results The results revealed lower frequencies of IFNγ-secreting (p = 0.02) and higher frequencies of IL-4-secreting (p = 0.03) CD4+ T-cells in patient lymphocyte cultures compared to healthy control cultures in the presence of polyclonal stimuli. This is the first description of differential cytokine patterns in Papular Urticaria patients. Conclusion Patients suffering from Papular Urticaria have an atopic status compared to healthy children.
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Differential Th1/Th2 balance in peripheral blood lymphocytes from patients suffering from flea bite-induced Papular Urticaria
Allergologia et immunopathologia, 2009Co-Authors: Adriana Cuéllar, Evelyne Halpert, Alberto Gomez, F. Rojas, Adriana Rodríguez, Elizabeth GarcíaAbstract:Abstract Background The Th1/Th2 balance has not been characterized in patients suffering from flea bite-induced Papular Urticaria (FBPU). Our aim was to improve understanding of the immunopathogenesis of CD4+ and CD8+ T-cells in humans suffering from flea bite-induced Papular Urticaria. Methods Peripheral blood mononuclear cells were obtained from 18 pediatric patients and 10 age-matched healthy controls. Cellular phenotypes, intracellular production of interferon gamma (IFNγ) and interleukin-4 (IL-4) in T-cells stimulated with polyclonal stimuli was determined by flow cytometry following short-term in vitro stimulation. Results The results revealed lower frequencies of IFNγ-secreting (p = 0.02) and higher frequencies of IL-4-secreting (p = 0.03) CD4+ T-cells in patient lymphocyte cultures compared to healthy control cultures in the presence of polyclonal stimuli. This is the first description of differential cytokine patterns in Papular Urticaria patients. Conclusion Patients suffering from Papular Urticaria have an atopic status compared to healthy children.
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Functional Dysregulation of Dendritic Cells in Patients With Papular Urticaria Caused by Fleabite
Archives of dermatology, 2007Co-Authors: Adriana Cuéllar, Evelyne Halpert, Elizabeth García, Adriana Rodríguez, Alberto GomezAbstract:Background Papular Urticaria is a chronic allergic disease caused by fleabite. The presence of eosinophils, predominance of CD4-positive T cells in lesions, and IgE response suggest a Th2 immune response to flea proteins in patients with Papular Urticaria caused by fleabite (PUFB). Although PUFB is defined as an allergic reaction, the immunological mechanisms and the role of dendritic cells (DCs) have not been established. Observations Flea body extract did not induce the maturation of monocyte-derived DCs in 10 patients with PUFB and in 10 healthy children. Simultaneous exposure of DCs to flea extract and lipopolysaccharide induced increased expression of CD83 ( P P P Conclusions Results of this study indicate that the involvement of DCs in an immune response produced in the disease is mediated through the altered expression of membrane molecules. This may be related to constitutive impairment in the production of regulatory cytokines such as IL-6 and IL-10 in these patients.
