The Experts below are selected from a list of 84 Experts worldwide ranked by ideXlab platform
Francois Marceau - One of the best experts on this subject based on the ideXlab platform.
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a tagged Parathyroid Hormone Derivative as a carrier of antibody cargoes transported by the g protein coupled pth1 receptor
Peptides, 2014Co-Authors: Xavier Charestmorin, Jeanphilippe Fortin, Robert Lodge, Isabelle Allaeys, Patrice E Poubelle, Francois MarceauAbstract:Abstract Based on the known fact that the Parathyroid Hormone (PTH) might be extended at its C-terminus with biotechnological protein cargoes, a vector directing the secretion of PTH 1–84 C-terminally fused with the antigenic epitope myc (PTH-myc) was exploited. The functional properties and potential of this analog for imaging PTH 1 R-expressing cells were examined. The PTH-myc construct was recombinantly produced as a conditioned medium (CM) of transfected HEK 293a cells (typical concentrations of 187 nM estimated with ELISAs for PTH). PTH-myc CM induced cyclic AMP formations (10 min), with a minor loss of potency relative to authentic PTH 1–84 , and c-Fos expression (1–3 h). Treatment of recipient HEK 293a cells transiently expressing PTH 1 R with PTH-myc CM (supplemented with a fluorescent monoclonal anti-myc tag antibody, either 4A6 or 9E10) allowed the labeling of endosomal structures positive for Rab5 and/or for β-arrestin 1 (microscopy, cytofluorometry). Authentic PTH was inactive in this respect, ruling out a non-specific form of endocytosis like pinocytosis. Using a horseradish peroxidase-conjugated secondary antibody, the endocytosis of the PTH-myc-based antibody complex by endogenous PTH 1 R was evidenced in MG-63 osteoblastoid cells. The secreted construct PTH-myc represents a bona fide agonist that supports the feasibility of transporting cargoes of considerable molecular weight inside cells using arrestin and Rab5-mediated PTH 1 R endocytosis. PTH-myc is also transported into cells that express PTH 1 R at a physiological level. Such tagged peptide Hormones may be part of a cancer chemotherapy scheme exploiting a modular cytotoxic secondary antibody and the receptor repertoire expressed in a given tumor.
Xavier Charestmorin - One of the best experts on this subject based on the ideXlab platform.
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a tagged Parathyroid Hormone Derivative as a carrier of antibody cargoes transported by the g protein coupled pth1 receptor
Peptides, 2014Co-Authors: Xavier Charestmorin, Jeanphilippe Fortin, Robert Lodge, Isabelle Allaeys, Patrice E Poubelle, Francois MarceauAbstract:Abstract Based on the known fact that the Parathyroid Hormone (PTH) might be extended at its C-terminus with biotechnological protein cargoes, a vector directing the secretion of PTH 1–84 C-terminally fused with the antigenic epitope myc (PTH-myc) was exploited. The functional properties and potential of this analog for imaging PTH 1 R-expressing cells were examined. The PTH-myc construct was recombinantly produced as a conditioned medium (CM) of transfected HEK 293a cells (typical concentrations of 187 nM estimated with ELISAs for PTH). PTH-myc CM induced cyclic AMP formations (10 min), with a minor loss of potency relative to authentic PTH 1–84 , and c-Fos expression (1–3 h). Treatment of recipient HEK 293a cells transiently expressing PTH 1 R with PTH-myc CM (supplemented with a fluorescent monoclonal anti-myc tag antibody, either 4A6 or 9E10) allowed the labeling of endosomal structures positive for Rab5 and/or for β-arrestin 1 (microscopy, cytofluorometry). Authentic PTH was inactive in this respect, ruling out a non-specific form of endocytosis like pinocytosis. Using a horseradish peroxidase-conjugated secondary antibody, the endocytosis of the PTH-myc-based antibody complex by endogenous PTH 1 R was evidenced in MG-63 osteoblastoid cells. The secreted construct PTH-myc represents a bona fide agonist that supports the feasibility of transporting cargoes of considerable molecular weight inside cells using arrestin and Rab5-mediated PTH 1 R endocytosis. PTH-myc is also transported into cells that express PTH 1 R at a physiological level. Such tagged peptide Hormones may be part of a cancer chemotherapy scheme exploiting a modular cytotoxic secondary antibody and the receptor repertoire expressed in a given tumor.
Robert Lodge - One of the best experts on this subject based on the ideXlab platform.
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a tagged Parathyroid Hormone Derivative as a carrier of antibody cargoes transported by the g protein coupled pth1 receptor
Peptides, 2014Co-Authors: Xavier Charestmorin, Jeanphilippe Fortin, Robert Lodge, Isabelle Allaeys, Patrice E Poubelle, Francois MarceauAbstract:Abstract Based on the known fact that the Parathyroid Hormone (PTH) might be extended at its C-terminus with biotechnological protein cargoes, a vector directing the secretion of PTH 1–84 C-terminally fused with the antigenic epitope myc (PTH-myc) was exploited. The functional properties and potential of this analog for imaging PTH 1 R-expressing cells were examined. The PTH-myc construct was recombinantly produced as a conditioned medium (CM) of transfected HEK 293a cells (typical concentrations of 187 nM estimated with ELISAs for PTH). PTH-myc CM induced cyclic AMP formations (10 min), with a minor loss of potency relative to authentic PTH 1–84 , and c-Fos expression (1–3 h). Treatment of recipient HEK 293a cells transiently expressing PTH 1 R with PTH-myc CM (supplemented with a fluorescent monoclonal anti-myc tag antibody, either 4A6 or 9E10) allowed the labeling of endosomal structures positive for Rab5 and/or for β-arrestin 1 (microscopy, cytofluorometry). Authentic PTH was inactive in this respect, ruling out a non-specific form of endocytosis like pinocytosis. Using a horseradish peroxidase-conjugated secondary antibody, the endocytosis of the PTH-myc-based antibody complex by endogenous PTH 1 R was evidenced in MG-63 osteoblastoid cells. The secreted construct PTH-myc represents a bona fide agonist that supports the feasibility of transporting cargoes of considerable molecular weight inside cells using arrestin and Rab5-mediated PTH 1 R endocytosis. PTH-myc is also transported into cells that express PTH 1 R at a physiological level. Such tagged peptide Hormones may be part of a cancer chemotherapy scheme exploiting a modular cytotoxic secondary antibody and the receptor repertoire expressed in a given tumor.
Patrice E Poubelle - One of the best experts on this subject based on the ideXlab platform.
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a tagged Parathyroid Hormone Derivative as a carrier of antibody cargoes transported by the g protein coupled pth1 receptor
Peptides, 2014Co-Authors: Xavier Charestmorin, Jeanphilippe Fortin, Robert Lodge, Isabelle Allaeys, Patrice E Poubelle, Francois MarceauAbstract:Abstract Based on the known fact that the Parathyroid Hormone (PTH) might be extended at its C-terminus with biotechnological protein cargoes, a vector directing the secretion of PTH 1–84 C-terminally fused with the antigenic epitope myc (PTH-myc) was exploited. The functional properties and potential of this analog for imaging PTH 1 R-expressing cells were examined. The PTH-myc construct was recombinantly produced as a conditioned medium (CM) of transfected HEK 293a cells (typical concentrations of 187 nM estimated with ELISAs for PTH). PTH-myc CM induced cyclic AMP formations (10 min), with a minor loss of potency relative to authentic PTH 1–84 , and c-Fos expression (1–3 h). Treatment of recipient HEK 293a cells transiently expressing PTH 1 R with PTH-myc CM (supplemented with a fluorescent monoclonal anti-myc tag antibody, either 4A6 or 9E10) allowed the labeling of endosomal structures positive for Rab5 and/or for β-arrestin 1 (microscopy, cytofluorometry). Authentic PTH was inactive in this respect, ruling out a non-specific form of endocytosis like pinocytosis. Using a horseradish peroxidase-conjugated secondary antibody, the endocytosis of the PTH-myc-based antibody complex by endogenous PTH 1 R was evidenced in MG-63 osteoblastoid cells. The secreted construct PTH-myc represents a bona fide agonist that supports the feasibility of transporting cargoes of considerable molecular weight inside cells using arrestin and Rab5-mediated PTH 1 R endocytosis. PTH-myc is also transported into cells that express PTH 1 R at a physiological level. Such tagged peptide Hormones may be part of a cancer chemotherapy scheme exploiting a modular cytotoxic secondary antibody and the receptor repertoire expressed in a given tumor.
Isabelle Allaeys - One of the best experts on this subject based on the ideXlab platform.
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a tagged Parathyroid Hormone Derivative as a carrier of antibody cargoes transported by the g protein coupled pth1 receptor
Peptides, 2014Co-Authors: Xavier Charestmorin, Jeanphilippe Fortin, Robert Lodge, Isabelle Allaeys, Patrice E Poubelle, Francois MarceauAbstract:Abstract Based on the known fact that the Parathyroid Hormone (PTH) might be extended at its C-terminus with biotechnological protein cargoes, a vector directing the secretion of PTH 1–84 C-terminally fused with the antigenic epitope myc (PTH-myc) was exploited. The functional properties and potential of this analog for imaging PTH 1 R-expressing cells were examined. The PTH-myc construct was recombinantly produced as a conditioned medium (CM) of transfected HEK 293a cells (typical concentrations of 187 nM estimated with ELISAs for PTH). PTH-myc CM induced cyclic AMP formations (10 min), with a minor loss of potency relative to authentic PTH 1–84 , and c-Fos expression (1–3 h). Treatment of recipient HEK 293a cells transiently expressing PTH 1 R with PTH-myc CM (supplemented with a fluorescent monoclonal anti-myc tag antibody, either 4A6 or 9E10) allowed the labeling of endosomal structures positive for Rab5 and/or for β-arrestin 1 (microscopy, cytofluorometry). Authentic PTH was inactive in this respect, ruling out a non-specific form of endocytosis like pinocytosis. Using a horseradish peroxidase-conjugated secondary antibody, the endocytosis of the PTH-myc-based antibody complex by endogenous PTH 1 R was evidenced in MG-63 osteoblastoid cells. The secreted construct PTH-myc represents a bona fide agonist that supports the feasibility of transporting cargoes of considerable molecular weight inside cells using arrestin and Rab5-mediated PTH 1 R endocytosis. PTH-myc is also transported into cells that express PTH 1 R at a physiological level. Such tagged peptide Hormones may be part of a cancer chemotherapy scheme exploiting a modular cytotoxic secondary antibody and the receptor repertoire expressed in a given tumor.
