The Experts below are selected from a list of 267 Experts worldwide ranked by ideXlab platform

Jason Adamson - One of the best experts on this subject based on the ideXlab platform.

  • Characterisation of a Vitrocell® VC 10 in vitro smoke exposure system using dose tools and biological analysis
    Chemistry Central Journal, 2013
    Co-Authors: David Thorne, Joanne Kilford, Rebecca Payne, Jason Adamson, Ken Scott, Annette Dalrymple, Clive Meredith, Deborah Dillon
    Abstract:

    The development of whole smoke exposure systems have been driven by the fact that traditional smoke exposure techniques are based on the Particulate phase of tobacco smoke and not the complete smoke aerosol. To overcome these challenges in this study, we used a Vitrocell® VC 10 whole smoke exposure system. For characterisation purposes, we determined smoke Deposition in relationship to airflow (L/min), regional smoke Deposition within the linear exposure module, vapour phase dilution using a known smoke marker (carbon monoxide) and finally assessed biological responses using two independent biological systems, the Ames and Neutral Red uptake (NRU) assay. Smoke dilution correlates with Particulate Deposition (R2 = 0.97) and CO concentration (R2 = 0.98). Regional Deposition analysis within the linear exposure chamber showed no statistical difference in deposited mass across the chamber at any airflows tested. Biological analysis showed consistent responses and positive correlations with deposited mass for both the Ames (R2 = 0.76) and NRU (R2 = 0.84) assays. We conclude that in our study, under the experimental conditions tested, the VC 10 can produce stable tobacco smoke dilutions, as demonstrated by Particulate Deposition, measured vapour phase smoke marker delivery and biological responses from two independent in vitro test systems.

  • Real-time assessment of cigarette smoke particle Deposition in vitro
    Chemistry Central journal, 2012
    Co-Authors: Jason Adamson, Sophie Hughes, David Azzopardi, John Mcaughey, Marianna Gaça
    Abstract:

    Recently there has been a rapid increase in approaches to assess the effects of cigarette smoke in vitro. Despite a range of gravimetric and chemical methods, there is a requirement to identify simpler and more reliable methods to quantify in vitro whole smoke dose, to support extrapolation and comparisons to human/in vivo dose. We have previously characterised an in vitro exposure system using a Borgwaldt RM20S smoking machine and a chamber exposing cellular cultures to whole smoke at the air-liquid interface. In this study we demonstrate the utility of a quartz crystal microbalance (QCM), using this exposure system, to assess real-time cigarette smoke Particulate Deposition during a 30 minute smoke exposure. Smoke was generated at various dilutions (1:5–1:400, smoke:air) using two cigarette products, 3R4F Kentucky reference and 1 mg commercially available cigarettes. The QCM, integrated into the chamber, assessed Particulate Deposition and data generated were compared to traditional chemical spectrofluorometric analysis. The QCM chamber was able to detect mass differences between the different products within the nanogram range. 3R4F reference cigarette smoke Deposition ranged from 25.75 ±2.30 μg/cm2 (1:5) to 0.22 ±0.03 μg/cm2 (1:400). 1 mg cigarette smoke Deposition was less and ranged from 1.42 ±0.26 μg/cm2 (1:5), to 0.13 ±0.02 μg/cm2 (1:100). Spectrofluorometric analysis demonstrated statistically significant correlation of Particulate Deposition with the QCM (p 

  • Real-time assessment of cigarette smoke particle Deposition in vitro
    Chemistry Central Journal, 2012
    Co-Authors: Jason Adamson, Sophie Hughes, David Azzopardi, John Mcaughey, Marianna Gaça
    Abstract:

    Background Recently there has been a rapid increase in approaches to assess the effects of cigarette smoke in vitro . Despite a range of gravimetric and chemical methods, there is a requirement to identify simpler and more reliable methods to quantify in vitro whole smoke dose, to support extrapolation and comparisons to human/ in vivo dose. We have previously characterised an in vitro exposure system using a Borgwaldt RM20S smoking machine and a chamber exposing cellular cultures to whole smoke at the air-liquid interface. In this study we demonstrate the utility of a quartz crystal microbalance (QCM), using this exposure system, to assess real-time cigarette smoke Particulate Deposition during a 30 minute smoke exposure. Smoke was generated at various dilutions (1:5–1:400, smoke:air) using two cigarette products, 3R4F Kentucky reference and 1 mg commercially available cigarettes. The QCM, integrated into the chamber, assessed Particulate Deposition and data generated were compared to traditional chemical spectrofluorometric analysis. Results The QCM chamber was able to detect mass differences between the different products within the nanogram range. 3R4F reference cigarette smoke Deposition ranged from 25.75 ±2.30 μg/cm^2 (1:5) to 0.22 ±0.03 μg/cm^2 (1:400). 1 mg cigarette smoke Deposition was less and ranged from 1.42 ±0.26 μg/cm^2 (1:5), to 0.13 ±0.02 μg/cm^2 (1:100). Spectrofluorometric analysis demonstrated statistically significant correlation of Particulate Deposition with the QCM (p 

Marianna Gaça - One of the best experts on this subject based on the ideXlab platform.

  • Real-time assessment of cigarette smoke particle Deposition in vitro
    Chemistry Central journal, 2012
    Co-Authors: Jason Adamson, Sophie Hughes, David Azzopardi, John Mcaughey, Marianna Gaça
    Abstract:

    Recently there has been a rapid increase in approaches to assess the effects of cigarette smoke in vitro. Despite a range of gravimetric and chemical methods, there is a requirement to identify simpler and more reliable methods to quantify in vitro whole smoke dose, to support extrapolation and comparisons to human/in vivo dose. We have previously characterised an in vitro exposure system using a Borgwaldt RM20S smoking machine and a chamber exposing cellular cultures to whole smoke at the air-liquid interface. In this study we demonstrate the utility of a quartz crystal microbalance (QCM), using this exposure system, to assess real-time cigarette smoke Particulate Deposition during a 30 minute smoke exposure. Smoke was generated at various dilutions (1:5–1:400, smoke:air) using two cigarette products, 3R4F Kentucky reference and 1 mg commercially available cigarettes. The QCM, integrated into the chamber, assessed Particulate Deposition and data generated were compared to traditional chemical spectrofluorometric analysis. The QCM chamber was able to detect mass differences between the different products within the nanogram range. 3R4F reference cigarette smoke Deposition ranged from 25.75 ±2.30 μg/cm2 (1:5) to 0.22 ±0.03 μg/cm2 (1:400). 1 mg cigarette smoke Deposition was less and ranged from 1.42 ±0.26 μg/cm2 (1:5), to 0.13 ±0.02 μg/cm2 (1:100). Spectrofluorometric analysis demonstrated statistically significant correlation of Particulate Deposition with the QCM (p 

  • Real-time assessment of cigarette smoke particle Deposition in vitro
    Chemistry Central Journal, 2012
    Co-Authors: Jason Adamson, Sophie Hughes, David Azzopardi, John Mcaughey, Marianna Gaça
    Abstract:

    Background Recently there has been a rapid increase in approaches to assess the effects of cigarette smoke in vitro . Despite a range of gravimetric and chemical methods, there is a requirement to identify simpler and more reliable methods to quantify in vitro whole smoke dose, to support extrapolation and comparisons to human/ in vivo dose. We have previously characterised an in vitro exposure system using a Borgwaldt RM20S smoking machine and a chamber exposing cellular cultures to whole smoke at the air-liquid interface. In this study we demonstrate the utility of a quartz crystal microbalance (QCM), using this exposure system, to assess real-time cigarette smoke Particulate Deposition during a 30 minute smoke exposure. Smoke was generated at various dilutions (1:5–1:400, smoke:air) using two cigarette products, 3R4F Kentucky reference and 1 mg commercially available cigarettes. The QCM, integrated into the chamber, assessed Particulate Deposition and data generated were compared to traditional chemical spectrofluorometric analysis. Results The QCM chamber was able to detect mass differences between the different products within the nanogram range. 3R4F reference cigarette smoke Deposition ranged from 25.75 ±2.30 μg/cm^2 (1:5) to 0.22 ±0.03 μg/cm^2 (1:400). 1 mg cigarette smoke Deposition was less and ranged from 1.42 ±0.26 μg/cm^2 (1:5), to 0.13 ±0.02 μg/cm^2 (1:100). Spectrofluorometric analysis demonstrated statistically significant correlation of Particulate Deposition with the QCM (p 

John Mcaughey - One of the best experts on this subject based on the ideXlab platform.

  • Real-time assessment of cigarette smoke particle Deposition in vitro
    Chemistry Central journal, 2012
    Co-Authors: Jason Adamson, Sophie Hughes, David Azzopardi, John Mcaughey, Marianna Gaça
    Abstract:

    Recently there has been a rapid increase in approaches to assess the effects of cigarette smoke in vitro. Despite a range of gravimetric and chemical methods, there is a requirement to identify simpler and more reliable methods to quantify in vitro whole smoke dose, to support extrapolation and comparisons to human/in vivo dose. We have previously characterised an in vitro exposure system using a Borgwaldt RM20S smoking machine and a chamber exposing cellular cultures to whole smoke at the air-liquid interface. In this study we demonstrate the utility of a quartz crystal microbalance (QCM), using this exposure system, to assess real-time cigarette smoke Particulate Deposition during a 30 minute smoke exposure. Smoke was generated at various dilutions (1:5–1:400, smoke:air) using two cigarette products, 3R4F Kentucky reference and 1 mg commercially available cigarettes. The QCM, integrated into the chamber, assessed Particulate Deposition and data generated were compared to traditional chemical spectrofluorometric analysis. The QCM chamber was able to detect mass differences between the different products within the nanogram range. 3R4F reference cigarette smoke Deposition ranged from 25.75 ±2.30 μg/cm2 (1:5) to 0.22 ±0.03 μg/cm2 (1:400). 1 mg cigarette smoke Deposition was less and ranged from 1.42 ±0.26 μg/cm2 (1:5), to 0.13 ±0.02 μg/cm2 (1:100). Spectrofluorometric analysis demonstrated statistically significant correlation of Particulate Deposition with the QCM (p 

  • Real-time assessment of cigarette smoke particle Deposition in vitro
    Chemistry Central Journal, 2012
    Co-Authors: Jason Adamson, Sophie Hughes, David Azzopardi, John Mcaughey, Marianna Gaça
    Abstract:

    Background Recently there has been a rapid increase in approaches to assess the effects of cigarette smoke in vitro . Despite a range of gravimetric and chemical methods, there is a requirement to identify simpler and more reliable methods to quantify in vitro whole smoke dose, to support extrapolation and comparisons to human/ in vivo dose. We have previously characterised an in vitro exposure system using a Borgwaldt RM20S smoking machine and a chamber exposing cellular cultures to whole smoke at the air-liquid interface. In this study we demonstrate the utility of a quartz crystal microbalance (QCM), using this exposure system, to assess real-time cigarette smoke Particulate Deposition during a 30 minute smoke exposure. Smoke was generated at various dilutions (1:5–1:400, smoke:air) using two cigarette products, 3R4F Kentucky reference and 1 mg commercially available cigarettes. The QCM, integrated into the chamber, assessed Particulate Deposition and data generated were compared to traditional chemical spectrofluorometric analysis. Results The QCM chamber was able to detect mass differences between the different products within the nanogram range. 3R4F reference cigarette smoke Deposition ranged from 25.75 ±2.30 μg/cm^2 (1:5) to 0.22 ±0.03 μg/cm^2 (1:400). 1 mg cigarette smoke Deposition was less and ranged from 1.42 ±0.26 μg/cm^2 (1:5), to 0.13 ±0.02 μg/cm^2 (1:100). Spectrofluorometric analysis demonstrated statistically significant correlation of Particulate Deposition with the QCM (p 

Sophie Hughes - One of the best experts on this subject based on the ideXlab platform.

  • Real-time assessment of cigarette smoke particle Deposition in vitro
    Chemistry Central journal, 2012
    Co-Authors: Jason Adamson, Sophie Hughes, David Azzopardi, John Mcaughey, Marianna Gaça
    Abstract:

    Recently there has been a rapid increase in approaches to assess the effects of cigarette smoke in vitro. Despite a range of gravimetric and chemical methods, there is a requirement to identify simpler and more reliable methods to quantify in vitro whole smoke dose, to support extrapolation and comparisons to human/in vivo dose. We have previously characterised an in vitro exposure system using a Borgwaldt RM20S smoking machine and a chamber exposing cellular cultures to whole smoke at the air-liquid interface. In this study we demonstrate the utility of a quartz crystal microbalance (QCM), using this exposure system, to assess real-time cigarette smoke Particulate Deposition during a 30 minute smoke exposure. Smoke was generated at various dilutions (1:5–1:400, smoke:air) using two cigarette products, 3R4F Kentucky reference and 1 mg commercially available cigarettes. The QCM, integrated into the chamber, assessed Particulate Deposition and data generated were compared to traditional chemical spectrofluorometric analysis. The QCM chamber was able to detect mass differences between the different products within the nanogram range. 3R4F reference cigarette smoke Deposition ranged from 25.75 ±2.30 μg/cm2 (1:5) to 0.22 ±0.03 μg/cm2 (1:400). 1 mg cigarette smoke Deposition was less and ranged from 1.42 ±0.26 μg/cm2 (1:5), to 0.13 ±0.02 μg/cm2 (1:100). Spectrofluorometric analysis demonstrated statistically significant correlation of Particulate Deposition with the QCM (p 

  • Real-time assessment of cigarette smoke particle Deposition in vitro
    Chemistry Central Journal, 2012
    Co-Authors: Jason Adamson, Sophie Hughes, David Azzopardi, John Mcaughey, Marianna Gaça
    Abstract:

    Background Recently there has been a rapid increase in approaches to assess the effects of cigarette smoke in vitro . Despite a range of gravimetric and chemical methods, there is a requirement to identify simpler and more reliable methods to quantify in vitro whole smoke dose, to support extrapolation and comparisons to human/ in vivo dose. We have previously characterised an in vitro exposure system using a Borgwaldt RM20S smoking machine and a chamber exposing cellular cultures to whole smoke at the air-liquid interface. In this study we demonstrate the utility of a quartz crystal microbalance (QCM), using this exposure system, to assess real-time cigarette smoke Particulate Deposition during a 30 minute smoke exposure. Smoke was generated at various dilutions (1:5–1:400, smoke:air) using two cigarette products, 3R4F Kentucky reference and 1 mg commercially available cigarettes. The QCM, integrated into the chamber, assessed Particulate Deposition and data generated were compared to traditional chemical spectrofluorometric analysis. Results The QCM chamber was able to detect mass differences between the different products within the nanogram range. 3R4F reference cigarette smoke Deposition ranged from 25.75 ±2.30 μg/cm^2 (1:5) to 0.22 ±0.03 μg/cm^2 (1:400). 1 mg cigarette smoke Deposition was less and ranged from 1.42 ±0.26 μg/cm^2 (1:5), to 0.13 ±0.02 μg/cm^2 (1:100). Spectrofluorometric analysis demonstrated statistically significant correlation of Particulate Deposition with the QCM (p 

David Azzopardi - One of the best experts on this subject based on the ideXlab platform.

  • Real-time assessment of cigarette smoke particle Deposition in vitro
    Chemistry Central journal, 2012
    Co-Authors: Jason Adamson, Sophie Hughes, David Azzopardi, John Mcaughey, Marianna Gaça
    Abstract:

    Recently there has been a rapid increase in approaches to assess the effects of cigarette smoke in vitro. Despite a range of gravimetric and chemical methods, there is a requirement to identify simpler and more reliable methods to quantify in vitro whole smoke dose, to support extrapolation and comparisons to human/in vivo dose. We have previously characterised an in vitro exposure system using a Borgwaldt RM20S smoking machine and a chamber exposing cellular cultures to whole smoke at the air-liquid interface. In this study we demonstrate the utility of a quartz crystal microbalance (QCM), using this exposure system, to assess real-time cigarette smoke Particulate Deposition during a 30 minute smoke exposure. Smoke was generated at various dilutions (1:5–1:400, smoke:air) using two cigarette products, 3R4F Kentucky reference and 1 mg commercially available cigarettes. The QCM, integrated into the chamber, assessed Particulate Deposition and data generated were compared to traditional chemical spectrofluorometric analysis. The QCM chamber was able to detect mass differences between the different products within the nanogram range. 3R4F reference cigarette smoke Deposition ranged from 25.75 ±2.30 μg/cm2 (1:5) to 0.22 ±0.03 μg/cm2 (1:400). 1 mg cigarette smoke Deposition was less and ranged from 1.42 ±0.26 μg/cm2 (1:5), to 0.13 ±0.02 μg/cm2 (1:100). Spectrofluorometric analysis demonstrated statistically significant correlation of Particulate Deposition with the QCM (p 

  • Real-time assessment of cigarette smoke particle Deposition in vitro
    Chemistry Central Journal, 2012
    Co-Authors: Jason Adamson, Sophie Hughes, David Azzopardi, John Mcaughey, Marianna Gaça
    Abstract:

    Background Recently there has been a rapid increase in approaches to assess the effects of cigarette smoke in vitro . Despite a range of gravimetric and chemical methods, there is a requirement to identify simpler and more reliable methods to quantify in vitro whole smoke dose, to support extrapolation and comparisons to human/ in vivo dose. We have previously characterised an in vitro exposure system using a Borgwaldt RM20S smoking machine and a chamber exposing cellular cultures to whole smoke at the air-liquid interface. In this study we demonstrate the utility of a quartz crystal microbalance (QCM), using this exposure system, to assess real-time cigarette smoke Particulate Deposition during a 30 minute smoke exposure. Smoke was generated at various dilutions (1:5–1:400, smoke:air) using two cigarette products, 3R4F Kentucky reference and 1 mg commercially available cigarettes. The QCM, integrated into the chamber, assessed Particulate Deposition and data generated were compared to traditional chemical spectrofluorometric analysis. Results The QCM chamber was able to detect mass differences between the different products within the nanogram range. 3R4F reference cigarette smoke Deposition ranged from 25.75 ±2.30 μg/cm^2 (1:5) to 0.22 ±0.03 μg/cm^2 (1:400). 1 mg cigarette smoke Deposition was less and ranged from 1.42 ±0.26 μg/cm^2 (1:5), to 0.13 ±0.02 μg/cm^2 (1:100). Spectrofluorometric analysis demonstrated statistically significant correlation of Particulate Deposition with the QCM (p