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Kenneth E. Blick - One of the best experts on this subject based on the ideXlab platform.

  • Passive Hemagglutination InhibitionTest for Diagnosis of Brown Recluse Spider Bite
    2014
    Co-Authors: Steven M. Barrett, Maxine Romine-jenkins, Kenneth E. Blick
    Abstract:

    Our goal was to recreate a Passive Hemagglutination inhibition (PHAI) test to diagnose brown recluse spider (BRS; Loxosc&es reclusa) bite envenomation for treat-ment trials. Guinea pigs received intradermal injectionsof concentrated spider venom from the following species: Loxosceles reclusa, Argiope aurantia, Argiope trifasciata, Phidippus audax, and Lycosa frondicola. Skin lesion ex-udate was collected and tested with the BRS venom PHAI assay. From 51 separate collections of exudate, test sen-sitivity was 90 % as long as 3 days after venom injection. Specfficity was 100 % with venom from the other spider species listedabove invivo (7 test samples) and invitro (5 test samples), as well as with random bacterial exudate with and without added serial dilutions of BRS venom (10 test samples). The test was reproducible over repetitiv

  • Passive Hemagglutination inhibition test for diagnosis of brown recluse spider bite envenomation
    Clinical Chemistry, 1993
    Co-Authors: Steven M. Barrett, M Rominejenkins, Kenneth E. Blick
    Abstract:

    Our goal was to recreate a Passive Hemagglutination inhibition (PHAI) test to diagnose brown recluse spider (BRS; Loxosceles reclusa) bite envenomation for treatment trials. Guinea pigs received intradermal injections of concentrated spider venom from the following species: Loxosceles reclusa, Argiope aurantia, Argiope trifasciata, Phidippus audax, and Lycosa frondicola. Skin lesion exudate was collected and tested with the BRS venom PHAI assay. From 51 separate collections of exudate, test sensitivity was 90% as long as 3 days after venom injection. Specificity was 100% with venom from the other spider species listed above in vivo (7 test samples) and in vitro (5 test samples), as well as with random bacterial exudate with and without added serial dilutions of BRS venom (10 test samples). The test was reproducible over repetitive assays to within one 10-fold dilution. A positive PHAI test result could function as an entry criterion for BRS bite victims in human treatment trials.

Helge Boman - One of the best experts on this subject based on the ideXlab platform.

  • studies on inherited antigenic variation of human serum β lipoprotein by Passive Hemagglutination
    International Journal of Protein Research, 2009
    Co-Authors: Helge Boman, Marit Hornberg Solaas
    Abstract:

    A methodological study on Passive Hemagglutination and Passive Hemagglutination inhibition reactions with human serum β-lipoprotein coupled to human red cells by bis-diazotized benzidine has been performed. Several variable factors have been studied, and a standardized procedure for optimal, reproducible results is described. A diluent containing dextrose which was found superior to several other diluents examined is described. Genetical differences in purified β-lipoprotein were clearly demonstrated by the Passive Hemagglutination technique using human isoantisera, and routine typing can be performed by inhibition experiments. The Passive Hemagglutination and the Hemagglutination inhibition tests described are specific and highly sensitive and are expected to become important supplements to the immunoprecipitation technique in the study of inherited β-lipoprotein variants. This work was supported by grants from J. L. Tiedemanns Tobaksfabrik, Joh. H. Andresens medisinske fond, C. H. Homans legat og legatet til Henrik Homans Minde, and Dahls legat to the author, as well as by grants from The Norwegian Research Council for Science and the Humanities, and from Nordisk Insulinfond, to Professor Kare Berg.

  • Studies on inherited antigenic variati on of human serum ß‐lipoprotein by Passive Hemagglutination
    Clinical genetics, 2008
    Co-Authors: Helge Boman, Hans Klausen
    Abstract:

    Sera from 101 multiparous (3 births or more) women and from 70 unselected normal individuals of both sexes have been tested by the Passive Hemagglutination method against red blood cells coated with ^-lipoprotein. Neither antibodies to β-lipoprotein antigens, nor anti-“Ag(e)” antibodies were detected. Thus, a previous observation of isoimmunization during pregnancies with inherited β-lipoprotein antigens was not confirmed.

  • Studies on inherited antigenic variation of human serum ß-lipoprotein by Passive Hemagglutination
    Clinical genetics, 2008
    Co-Authors: Helge Boman, Kåre Berg, Per Stavem
    Abstract:

    Sera from 39 unselected children with thalassaemia who had received multiple blood transfusions were examined for the presence of isoantibodies to β-lipoprotein antigens. Precipitating antibodies were found in 8 sera. In 5 of these, additional non-precipitating antibodies were detected by the Passive Hemagglutination method. In another 7 serum samples, only non-precipitating antibodies were present. Thus, 15 out of 39 sera (38.5 %) contained isoantibodies to β-lipoprotein antigens. This frequency of antibodies to β-lipoprotein antigens in patients with thalassaemia is the highest ever reported for any group of multiply-transfused patients. A total of 30 antibodies were found in these 15 sera.

Steven M. Barrett - One of the best experts on this subject based on the ideXlab platform.

  • Passive Hemagglutination InhibitionTest for Diagnosis of Brown Recluse Spider Bite
    2014
    Co-Authors: Steven M. Barrett, Maxine Romine-jenkins, Kenneth E. Blick
    Abstract:

    Our goal was to recreate a Passive Hemagglutination inhibition (PHAI) test to diagnose brown recluse spider (BRS; Loxosc&es reclusa) bite envenomation for treat-ment trials. Guinea pigs received intradermal injectionsof concentrated spider venom from the following species: Loxosceles reclusa, Argiope aurantia, Argiope trifasciata, Phidippus audax, and Lycosa frondicola. Skin lesion ex-udate was collected and tested with the BRS venom PHAI assay. From 51 separate collections of exudate, test sen-sitivity was 90 % as long as 3 days after venom injection. Specfficity was 100 % with venom from the other spider species listedabove invivo (7 test samples) and invitro (5 test samples), as well as with random bacterial exudate with and without added serial dilutions of BRS venom (10 test samples). The test was reproducible over repetitiv

  • Passive Hemagglutination inhibition test for diagnosis of brown recluse spider bite envenomation
    Clinical Chemistry, 1993
    Co-Authors: Steven M. Barrett, M Rominejenkins, Kenneth E. Blick
    Abstract:

    Our goal was to recreate a Passive Hemagglutination inhibition (PHAI) test to diagnose brown recluse spider (BRS; Loxosceles reclusa) bite envenomation for treatment trials. Guinea pigs received intradermal injections of concentrated spider venom from the following species: Loxosceles reclusa, Argiope aurantia, Argiope trifasciata, Phidippus audax, and Lycosa frondicola. Skin lesion exudate was collected and tested with the BRS venom PHAI assay. From 51 separate collections of exudate, test sensitivity was 90% as long as 3 days after venom injection. Specificity was 100% with venom from the other spider species listed above in vivo (7 test samples) and in vitro (5 test samples), as well as with random bacterial exudate with and without added serial dilutions of BRS venom (10 test samples). The test was reproducible over repetitive assays to within one 10-fold dilution. A positive PHAI test result could function as an entry criterion for BRS bite victims in human treatment trials.

Hans Klausen - One of the best experts on this subject based on the ideXlab platform.

Per Stavem - One of the best experts on this subject based on the ideXlab platform.

  • Studies on inherited antigenic variation of human serum ß-lipoprotein by Passive Hemagglutination
    Clinical genetics, 2008
    Co-Authors: Helge Boman, Kåre Berg, Per Stavem
    Abstract:

    Sera from 39 unselected children with thalassaemia who had received multiple blood transfusions were examined for the presence of isoantibodies to β-lipoprotein antigens. Precipitating antibodies were found in 8 sera. In 5 of these, additional non-precipitating antibodies were detected by the Passive Hemagglutination method. In another 7 serum samples, only non-precipitating antibodies were present. Thus, 15 out of 39 sera (38.5 %) contained isoantibodies to β-lipoprotein antigens. This frequency of antibodies to β-lipoprotein antigens in patients with thalassaemia is the highest ever reported for any group of multiply-transfused patients. A total of 30 antibodies were found in these 15 sera.