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Luca Bargelloni - One of the best experts on this subject based on the ideXlab platform.
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Quantitative trait loci for resistance to fish Pasteurellosis in gilthead sea bream (Sparus aurata).
Animal genetics, 2010Co-Authors: C. Massault, Rafaella Franch, Chris Haley, Dj De Koning, H. Bovenhuis, Caterina Pellizzari, Tomaso Patarnello, Luca BargelloniAbstract:Fish Pasteurellosis is a bacterial disease causing important losses in farmed fish, including gilthead sea bream, a teleost fish of great relevance in marine aquaculture. We report in this study a QTL analysis for resistance to fish Pasteurellosis in this species. An experimental population of 500 offspring originating from eight sires and six dams in a single mass-spawning event was subjected to a disease challenge with Photobacterium damselae subsp. piscicida (Phdp), the causative agent of fish Pasteurellosis. A total of 151 microsatellite loci were genotyped in the experimental population, and half-sib regression QTL analysis was carried out on two continuous traits, body length at time of death and survival, and for two binary traits, survival at day 7 and survival at day 15, when the highest peaks of mortality were observed. Two significant QTLs were detected for disease resistance. The first one was located on linkage group LG3 affecting late survival (survival at day 15). The second one, for overall survival, was located on LG21, which allowed us to highlight a potential marker (Id13) linked to disease resistance. A significant QTL was also found for body length at death on LG6 explaining 5–8% of the phenotypic variation.
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estimates of heritability and genetic correlation for body length and resistance to fish Pasteurellosis in the gilthead sea bream sparus aurata l
Aquaculture, 2009Co-Authors: Jenny Antonello, C. Massault, Rafaella Franch, Chris Haley, Caterina Pellizzari, Tomaso Patarnello, Giuseppe Bovo, Dirkjan De Koning, Luca BargelloniAbstract:Abstract Fish Pasteurellosis is an infectious disease that affects several fish species living in marine temperate waters. Its causative agent is the Gram-negative bacterium Photobacterium damselae subsp. piscicida (Phdp). Fish Pasteurellosis represents a serious health problem for the majority of intensive sea bream hatcheries, with 90–100% mortality during disease outbreaks. Larvae and juveniles are the most susceptible stages. A potential strategy to prevent fish Pasteurellosis is to select for animals that are genetically resistant to it. The aim of this work was to evaluate the genetic variation of disease resistance and growth in the gilthead sea bream (Sparus aurata L.). An experimental population, approximately 3500 animals originating from mass spawning of four broodstocks, was experimentally infected with a highly virulent strain of Phdp. Mortality was monitored daily for 19 days. Upon completion of the challenge experiment, genetic profiles at nine microsatellite loci were obtained for 1753 animals and for all (256) broodstock fish, for parentage assignment. A subset of families defined by means of genetic analysis was used to calculate heritability for survival to infection. For body length (measured post-mortem), heritability was 0.38 ± 0.07. The heritability for disease resistance varied depending on how resistance was defined. Heritability was 0.12 ± 0.04 for days of survival post challenge, defined as a continuous trait, while it ranged from 0.45 ± 0.04 to 0.18 ± 0.08 for the binary trait dead/alive at a specific day. The genetic correlation between body length and survival was positive and significant (r = 0.61 ± 0.16). These results confirm the existence of genetic variation for growth and resistance against Phpd and highlight also the potential for selective breeding programs to improve these traits.
C. Massault - One of the best experts on this subject based on the ideXlab platform.
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Quantitative trait loci for resistance to fish Pasteurellosis in gilthead sea bream (Sparus aurata).
Animal genetics, 2010Co-Authors: C. Massault, Rafaella Franch, Chris Haley, Dj De Koning, H. Bovenhuis, Caterina Pellizzari, Tomaso Patarnello, Luca BargelloniAbstract:Fish Pasteurellosis is a bacterial disease causing important losses in farmed fish, including gilthead sea bream, a teleost fish of great relevance in marine aquaculture. We report in this study a QTL analysis for resistance to fish Pasteurellosis in this species. An experimental population of 500 offspring originating from eight sires and six dams in a single mass-spawning event was subjected to a disease challenge with Photobacterium damselae subsp. piscicida (Phdp), the causative agent of fish Pasteurellosis. A total of 151 microsatellite loci were genotyped in the experimental population, and half-sib regression QTL analysis was carried out on two continuous traits, body length at time of death and survival, and for two binary traits, survival at day 7 and survival at day 15, when the highest peaks of mortality were observed. Two significant QTLs were detected for disease resistance. The first one was located on linkage group LG3 affecting late survival (survival at day 15). The second one, for overall survival, was located on LG21, which allowed us to highlight a potential marker (Id13) linked to disease resistance. A significant QTL was also found for body length at death on LG6 explaining 5–8% of the phenotypic variation.
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estimates of heritability and genetic correlation for body length and resistance to fish Pasteurellosis in the gilthead sea bream sparus aurata l
Aquaculture, 2009Co-Authors: Jenny Antonello, C. Massault, Rafaella Franch, Chris Haley, Caterina Pellizzari, Tomaso Patarnello, Giuseppe Bovo, Dirkjan De Koning, Luca BargelloniAbstract:Abstract Fish Pasteurellosis is an infectious disease that affects several fish species living in marine temperate waters. Its causative agent is the Gram-negative bacterium Photobacterium damselae subsp. piscicida (Phdp). Fish Pasteurellosis represents a serious health problem for the majority of intensive sea bream hatcheries, with 90–100% mortality during disease outbreaks. Larvae and juveniles are the most susceptible stages. A potential strategy to prevent fish Pasteurellosis is to select for animals that are genetically resistant to it. The aim of this work was to evaluate the genetic variation of disease resistance and growth in the gilthead sea bream (Sparus aurata L.). An experimental population, approximately 3500 animals originating from mass spawning of four broodstocks, was experimentally infected with a highly virulent strain of Phdp. Mortality was monitored daily for 19 days. Upon completion of the challenge experiment, genetic profiles at nine microsatellite loci were obtained for 1753 animals and for all (256) broodstock fish, for parentage assignment. A subset of families defined by means of genetic analysis was used to calculate heritability for survival to infection. For body length (measured post-mortem), heritability was 0.38 ± 0.07. The heritability for disease resistance varied depending on how resistance was defined. Heritability was 0.12 ± 0.04 for days of survival post challenge, defined as a continuous trait, while it ranged from 0.45 ± 0.04 to 0.18 ± 0.08 for the binary trait dead/alive at a specific day. The genetic correlation between body length and survival was positive and significant (r = 0.61 ± 0.16). These results confirm the existence of genetic variation for growth and resistance against Phpd and highlight also the potential for selective breeding programs to improve these traits.
Raphaël Robert - One of the best experts on this subject based on the ideXlab platform.
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Genetic parameters of resistance to Pasteurellosis using novel response traits in rabbits
Genetics Selection Evolution, 2020Co-Authors: Merina Shrestha, Herve Garreau, Elodie Balmisse, Ingrid David, Edouard Guitton, Emmanuelle Helloin, Guillaume Lenoir, Mickaël Maupin, Bertrand Bed’hom, Raphaël RobertAbstract:BackgroundPasteurellosis (Pasteurella infection) is one of the most common bacterial infections in rabbits on commercial farms and in laboratory facilities. Curative treatments using antibiotics are only partly efficient, with frequent relapses. Breeding rabbits for improved genetic resistance to Pasteurellosis is a sustainable alternative approach. In this study, we infected 964 crossbred rabbits from six sire lines experimentally with Pasteurella multocida. After post-mortem examination and bacteriological analyses, abscess, bacteria, and resistance scores were derived for each rabbit based on the extent of lesions and bacterial dissemination in the body. This is the first study to use such an experimental design and response traits to measure resistance to Pasteurellosis in a rabbit population. We investigated the genetic variation of these traits in order to identify potential selection criteria. We also estimated genetic correlations of resistance to Pasteurellosis in the experimental population with traits that are under selection in the breeding populations (number of kits born alive and weaning weight).ResultsHeritability estimates for the novel response traits, abscess, bacteria, and resistance scores, ranged from 0.08 (± 0.05) to 0.16 (± 0.06). The resistance score showed very strong negative genetic correlation estimates with abscess (− 0.99 ± 0.05) and bacteria scores (− 0.98 ± 0.07). A very high positive genetic correlation of 0.99 ± 0.16 was estimated between abscess and bacteria scores. Estimates of genetic correlations of the resistance score with average daily gain traits for the first and second week after inoculation were 0.98 (± 0.06) and 0.70 (± 0.14), respectively. Estimates of genetic correlations of the disease-related traits with average daily gain pre-inoculation were favorable but with high standard errors. Estimates of genetic and phenotypic correlations of the disease-related traits with commercial selection traits were not significantly different from zero.ConclusionsDisease response traits are heritable and are highly correlated with each other, but do not show any significant genetic correlations with commercial selection traits. Thus, the prevalence of Pasteurellosis could be decreased by selecting more resistant rabbits on any one of the disease response traits with a limited impact on the selection traits, which would allow implementation of a breeding program to improve resistance to Pasteurellosis in rabbits.
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Genetic parameters of resistance to Pasteurellosis using novel response traits in rabbits.
Genetics selection evolution : GSE, 2020Co-Authors: Merina Shrestha, Herve Garreau, Elodie Balmisse, Bertrand Bed'hom, Ingrid David, Edouard Guitton, Emmanuelle Helloin, Guillaume Lenoir, Mickaël Maupin, Raphaël RobertAbstract:Pasteurellosis (Pasteurella infection) is one of the most common bacterial infections in rabbits on commercial farms and in laboratory facilities. Curative treatments using antibiotics are only partly efficient, with frequent relapses. Breeding rabbits for improved genetic resistance to Pasteurellosis is a sustainable alternative approach. In this study, we infected 964 crossbred rabbits from six sire lines experimentally with Pasteurella multocida. After post-mortem examination and bacteriological analyses, abscess, bacteria, and resistance scores were derived for each rabbit based on the extent of lesions and bacterial dissemination in the body. This is the first study to use such an experimental design and response traits to measure resistance to Pasteurellosis in a rabbit population. We investigated the genetic variation of these traits in order to identify potential selection criteria. We also estimated genetic correlations of resistance to Pasteurellosis in the experimental population with traits that are under selection in the breeding populations (number of kits born alive and weaning weight). Heritability estimates for the novel response traits, abscess, bacteria, and resistance scores, ranged from 0.08 (± 0.05) to 0.16 (± 0.06). The resistance score showed very strong negative genetic correlation estimates with abscess (− 0.99 ± 0.05) and bacteria scores (− 0.98 ± 0.07). A very high positive genetic correlation of 0.99 ± 0.16 was estimated between abscess and bacteria scores. Estimates of genetic correlations of the resistance score with average daily gain traits for the first and second week after inoculation were 0.98 (± 0.06) and 0.70 (± 0.14), respectively. Estimates of genetic correlations of the disease-related traits with average daily gain pre-inoculation were favorable but with high standard errors. Estimates of genetic and phenotypic correlations of the disease-related traits with commercial selection traits were not significantly different from zero. Disease response traits are heritable and are highly correlated with each other, but do not show any significant genetic correlations with commercial selection traits. Thus, the prevalence of Pasteurellosis could be decreased by selecting more resistant rabbits on any one of the disease response traits with a limited impact on the selection traits, which would allow implementation of a breeding program to improve resistance to Pasteurellosis in rabbits.
Tomaso Patarnello - One of the best experts on this subject based on the ideXlab platform.
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Quantitative trait loci for resistance to fish Pasteurellosis in gilthead sea bream (Sparus aurata).
Animal genetics, 2010Co-Authors: C. Massault, Rafaella Franch, Chris Haley, Dj De Koning, H. Bovenhuis, Caterina Pellizzari, Tomaso Patarnello, Luca BargelloniAbstract:Fish Pasteurellosis is a bacterial disease causing important losses in farmed fish, including gilthead sea bream, a teleost fish of great relevance in marine aquaculture. We report in this study a QTL analysis for resistance to fish Pasteurellosis in this species. An experimental population of 500 offspring originating from eight sires and six dams in a single mass-spawning event was subjected to a disease challenge with Photobacterium damselae subsp. piscicida (Phdp), the causative agent of fish Pasteurellosis. A total of 151 microsatellite loci were genotyped in the experimental population, and half-sib regression QTL analysis was carried out on two continuous traits, body length at time of death and survival, and for two binary traits, survival at day 7 and survival at day 15, when the highest peaks of mortality were observed. Two significant QTLs were detected for disease resistance. The first one was located on linkage group LG3 affecting late survival (survival at day 15). The second one, for overall survival, was located on LG21, which allowed us to highlight a potential marker (Id13) linked to disease resistance. A significant QTL was also found for body length at death on LG6 explaining 5–8% of the phenotypic variation.
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estimates of heritability and genetic correlation for body length and resistance to fish Pasteurellosis in the gilthead sea bream sparus aurata l
Aquaculture, 2009Co-Authors: Jenny Antonello, C. Massault, Rafaella Franch, Chris Haley, Caterina Pellizzari, Tomaso Patarnello, Giuseppe Bovo, Dirkjan De Koning, Luca BargelloniAbstract:Abstract Fish Pasteurellosis is an infectious disease that affects several fish species living in marine temperate waters. Its causative agent is the Gram-negative bacterium Photobacterium damselae subsp. piscicida (Phdp). Fish Pasteurellosis represents a serious health problem for the majority of intensive sea bream hatcheries, with 90–100% mortality during disease outbreaks. Larvae and juveniles are the most susceptible stages. A potential strategy to prevent fish Pasteurellosis is to select for animals that are genetically resistant to it. The aim of this work was to evaluate the genetic variation of disease resistance and growth in the gilthead sea bream (Sparus aurata L.). An experimental population, approximately 3500 animals originating from mass spawning of four broodstocks, was experimentally infected with a highly virulent strain of Phdp. Mortality was monitored daily for 19 days. Upon completion of the challenge experiment, genetic profiles at nine microsatellite loci were obtained for 1753 animals and for all (256) broodstock fish, for parentage assignment. A subset of families defined by means of genetic analysis was used to calculate heritability for survival to infection. For body length (measured post-mortem), heritability was 0.38 ± 0.07. The heritability for disease resistance varied depending on how resistance was defined. Heritability was 0.12 ± 0.04 for days of survival post challenge, defined as a continuous trait, while it ranged from 0.45 ± 0.04 to 0.18 ± 0.08 for the binary trait dead/alive at a specific day. The genetic correlation between body length and survival was positive and significant (r = 0.61 ± 0.16). These results confirm the existence of genetic variation for growth and resistance against Phpd and highlight also the potential for selective breeding programs to improve these traits.
Caterina Pellizzari - One of the best experts on this subject based on the ideXlab platform.
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Quantitative trait loci for resistance to fish Pasteurellosis in gilthead sea bream (Sparus aurata).
Animal genetics, 2010Co-Authors: C. Massault, Rafaella Franch, Chris Haley, Dj De Koning, H. Bovenhuis, Caterina Pellizzari, Tomaso Patarnello, Luca BargelloniAbstract:Fish Pasteurellosis is a bacterial disease causing important losses in farmed fish, including gilthead sea bream, a teleost fish of great relevance in marine aquaculture. We report in this study a QTL analysis for resistance to fish Pasteurellosis in this species. An experimental population of 500 offspring originating from eight sires and six dams in a single mass-spawning event was subjected to a disease challenge with Photobacterium damselae subsp. piscicida (Phdp), the causative agent of fish Pasteurellosis. A total of 151 microsatellite loci were genotyped in the experimental population, and half-sib regression QTL analysis was carried out on two continuous traits, body length at time of death and survival, and for two binary traits, survival at day 7 and survival at day 15, when the highest peaks of mortality were observed. Two significant QTLs were detected for disease resistance. The first one was located on linkage group LG3 affecting late survival (survival at day 15). The second one, for overall survival, was located on LG21, which allowed us to highlight a potential marker (Id13) linked to disease resistance. A significant QTL was also found for body length at death on LG6 explaining 5–8% of the phenotypic variation.
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estimates of heritability and genetic correlation for body length and resistance to fish Pasteurellosis in the gilthead sea bream sparus aurata l
Aquaculture, 2009Co-Authors: Jenny Antonello, C. Massault, Rafaella Franch, Chris Haley, Caterina Pellizzari, Tomaso Patarnello, Giuseppe Bovo, Dirkjan De Koning, Luca BargelloniAbstract:Abstract Fish Pasteurellosis is an infectious disease that affects several fish species living in marine temperate waters. Its causative agent is the Gram-negative bacterium Photobacterium damselae subsp. piscicida (Phdp). Fish Pasteurellosis represents a serious health problem for the majority of intensive sea bream hatcheries, with 90–100% mortality during disease outbreaks. Larvae and juveniles are the most susceptible stages. A potential strategy to prevent fish Pasteurellosis is to select for animals that are genetically resistant to it. The aim of this work was to evaluate the genetic variation of disease resistance and growth in the gilthead sea bream (Sparus aurata L.). An experimental population, approximately 3500 animals originating from mass spawning of four broodstocks, was experimentally infected with a highly virulent strain of Phdp. Mortality was monitored daily for 19 days. Upon completion of the challenge experiment, genetic profiles at nine microsatellite loci were obtained for 1753 animals and for all (256) broodstock fish, for parentage assignment. A subset of families defined by means of genetic analysis was used to calculate heritability for survival to infection. For body length (measured post-mortem), heritability was 0.38 ± 0.07. The heritability for disease resistance varied depending on how resistance was defined. Heritability was 0.12 ± 0.04 for days of survival post challenge, defined as a continuous trait, while it ranged from 0.45 ± 0.04 to 0.18 ± 0.08 for the binary trait dead/alive at a specific day. The genetic correlation between body length and survival was positive and significant (r = 0.61 ± 0.16). These results confirm the existence of genetic variation for growth and resistance against Phpd and highlight also the potential for selective breeding programs to improve these traits.